Neodiprion abietis NPV
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q0ZNZ4|Q0ZNZ4_9CBAC Uncharacterized protein OS=Neodiprion abietis NPV OX=204507 PE=4 SV=1
MM1 pKa = 7.15 FLIVFVFLCILLIFFTHH18 pKa = 6.78 NIFVEE23 pKa = 4.5 TTDD26 pKa = 5.28 DD27 pKa = 3.41 VTKK30 pKa = 10.85 YY31 pKa = 10.56 KK32 pKa = 10.47 QIHH35 pKa = 5.9 NLLLEE40 pKa = 5.03 AYY42 pKa = 8.66 TSDD45 pKa = 3.44 LVSFDD50 pKa = 3.38 TVSFISRR57 pKa = 11.84 VRR59 pKa = 11.84 WPVYY63 pKa = 9.95 EE64 pKa = 3.67 IVTFEE69 pKa = 4.26 TEE71 pKa = 4.36 TYY73 pKa = 10.93 TMTNNMWYY81 pKa = 10.22 SGLEE85 pKa = 3.72 KK86 pKa = 10.51 RR87 pKa = 11.84 FNFYY91 pKa = 10.83 NQTFEE96 pKa = 5.61 DD97 pKa = 3.56 VDD99 pKa = 4.26 DD100 pKa = 4.07 NAEE103 pKa = 3.96 RR104 pKa = 11.84 VTTVDD109 pKa = 3.08 STSFSLHH116 pKa = 6.72 VDD118 pKa = 3.78 DD119 pKa = 5.42 GWVNVDD125 pKa = 4.36 CPANMIFDD133 pKa = 5.21 GYY135 pKa = 10.73 QCVRR139 pKa = 11.84 QNICEE144 pKa = 4.03 NQTAGTILPVNEE156 pKa = 4.32 ALIDD160 pKa = 3.4 VAFYY164 pKa = 10.92 QKK166 pKa = 10.9 YY167 pKa = 9.77 NRR169 pKa = 11.84 STTTDD174 pKa = 3.78 TIYY177 pKa = 10.88 HH178 pKa = 5.49 PTLYY182 pKa = 9.83 IVCNGDD188 pKa = 2.98 DD189 pKa = 3.36 TYY191 pKa = 11.36 EE192 pKa = 4.78 IKK194 pKa = 10.8 EE195 pKa = 4.22 CDD197 pKa = 3.35 NNQLFDD203 pKa = 4.76 SEE205 pKa = 4.53 MLQCVDD211 pKa = 4.57 KK212 pKa = 11.03 DD213 pKa = 3.45 ACADD217 pKa = 3.49 VADD220 pKa = 4.28 YY221 pKa = 10.35 FVLSDD226 pKa = 4.96 RR227 pKa = 11.84 PDD229 pKa = 3.29 TLADD233 pKa = 4.4 DD234 pKa = 4.76 EE235 pKa = 5.75 YY236 pKa = 11.89 LMCLNNEE243 pKa = 4.34 TTVQTCEE250 pKa = 3.53 EE251 pKa = 3.97 NMIFDD256 pKa = 4.43 TTTLSCVEE264 pKa = 4.05 VDD266 pKa = 3.67 LCQANGVGYY275 pKa = 8.32 TYY277 pKa = 9.62 ITDD280 pKa = 4.1 DD281 pKa = 3.74 LAANQYY287 pKa = 8.4 YY288 pKa = 9.75 RR289 pKa = 11.84 CTSVFDD295 pKa = 4.25 KK296 pKa = 10.98 EE297 pKa = 4.8 IVSCPIRR304 pKa = 11.84 VEE306 pKa = 3.72 TDD308 pKa = 3.01 GQYY311 pKa = 11.11 SCAGDD316 pKa = 3.92 TQCMSLEE323 pKa = 4.27 NGSGSIVTMTEE334 pKa = 3.6 NTHH337 pKa = 6.25 FKK339 pKa = 9.89 YY340 pKa = 10.75 ASEE343 pKa = 4.14 AVICTEE349 pKa = 4.3 YY350 pKa = 10.72 EE351 pKa = 3.76 ITDD354 pKa = 4.14 SIVCDD359 pKa = 3.72 SEE361 pKa = 4.64 EE362 pKa = 4.28 VEE364 pKa = 4.75 LSVEE368 pKa = 4.22 PFTLDD373 pKa = 3.43 LKK375 pKa = 10.97 IPTTILDD382 pKa = 3.59 RR383 pKa = 11.84 TNNVCKK389 pKa = 10.06 TFDD392 pKa = 3.49 INDD395 pKa = 3.32 VEE397 pKa = 5.22 IITDD401 pKa = 3.37 YY402 pKa = 11.59 AGIVNSEE409 pKa = 3.3 NDD411 pKa = 3.5 YY412 pKa = 11.57 NVDD415 pKa = 3.34 FTTAVRR421 pKa = 11.84 VNVQNLLSEE430 pKa = 4.39 NLPVSTSMLFSSLDD444 pKa = 3.32 YY445 pKa = 10.88 AKK447 pKa = 10.67 NYY449 pKa = 10.01 GFIGFSPLTGNEE461 pKa = 3.57 IDD463 pKa = 4.34 CYY465 pKa = 11.34 GDD467 pKa = 3.13 ILFDD471 pKa = 3.61 IFAGDD476 pKa = 3.85 KK477 pKa = 11.29 YY478 pKa = 11.08 NICSDD483 pKa = 3.91 NEE485 pKa = 4.04 ISSSNAIDD493 pKa = 4.02 DD494 pKa = 4.24 NEE496 pKa = 4.13 FFSTYY501 pKa = 8.04 TKK503 pKa = 11.03 NVGTTTLTSPCRR515 pKa = 11.84 VKK517 pKa = 10.98 SDD519 pKa = 3.15 IVLEE523 pKa = 4.04 DD524 pKa = 4.88 DD525 pKa = 4.88 IYY527 pKa = 10.99 IQEE530 pKa = 4.14 SDD532 pKa = 3.74 NVSVSCLYY540 pKa = 11.18 AMPLQEE546 pKa = 5.9 IIVTDD551 pKa = 4.04 LEE553 pKa = 4.13 EE554 pKa = 4.18 AAGVLIDD561 pKa = 5.45 DD562 pKa = 4.45 KK563 pKa = 11.29 CSEE566 pKa = 4.45 NGIDD570 pKa = 3.87 LNNVAGAIVILNDD583 pKa = 3.2 EE584 pKa = 4.31 LACRR588 pKa = 11.84 SVYY591 pKa = 10.89 LEE593 pKa = 4.92 DD594 pKa = 3.72 YY595 pKa = 10.71 NVIITASFTPTMKK608 pKa = 10.76 FEE610 pKa = 4.59 DD611 pKa = 3.96 RR612 pKa = 11.84 TWNVDD617 pKa = 3.19 TQSYY621 pKa = 10.09 DD622 pKa = 2.77 GVMYY626 pKa = 10.53 DD627 pKa = 3.92 SVVHH631 pKa = 6.89 KK632 pKa = 10.23 ITDD635 pKa = 2.99 GWMACPPSAYY645 pKa = 10.33 DD646 pKa = 4.93 KK647 pKa = 8.26 TTTDD651 pKa = 3.98 CNIDD655 pKa = 3.36 NTKK658 pKa = 10.77 LYY660 pKa = 9.11 TVKK663 pKa = 10.61 DD664 pKa = 3.16 LHH666 pKa = 6.65 FVQNYY671 pKa = 9.27 PDD673 pKa = 3.61 IDD675 pKa = 3.91 YY676 pKa = 11.07 EE677 pKa = 4.21 PEE679 pKa = 3.81 NEE681 pKa = 4.26 VNVDD685 pKa = 3.61 TEE687 pKa = 4.8 DD688 pKa = 3.75 VDD690 pKa = 3.98 VSNPIADD697 pKa = 3.88 DD698 pKa = 3.82 VEE700 pKa = 5.29 AINPIAEE707 pKa = 4.22 DD708 pKa = 3.52 VLRR711 pKa = 11.84 QNDD714 pKa = 3.46 NVTNNVQPALEE725 pKa = 4.23 NEE727 pKa = 4.6 LVNTATQMEE736 pKa = 4.66 VDD738 pKa = 3.87 QKK740 pKa = 11.24 NPSINVQDD748 pKa = 3.92 IDD750 pKa = 4.18 SSSTLSDD757 pKa = 3.06 EE758 pKa = 4.54 SVKK761 pKa = 10.78 NDD763 pKa = 3.41 YY764 pKa = 9.11 STLDD768 pKa = 3.28 TNRR771 pKa = 11.84 ILDD774 pKa = 4.06 YY775 pKa = 10.77 INRR778 pKa = 11.84 HH779 pKa = 5.78 HH780 pKa = 6.86 EE781 pKa = 4.28 SEE783 pKa = 4.36 TSEE786 pKa = 4.28 EE787 pKa = 4.46 EE788 pKa = 4.14 YY789 pKa = 10.48 MDD791 pKa = 5.57 DD792 pKa = 4.73 GDD794 pKa = 4.04 ILASLFEE801 pKa = 4.25
Molecular weight: 90.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.809
IPC2_protein 3.694
IPC_protein 3.745
Toseland 3.516
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.414
Solomon 3.732
Lehninger 3.681
Nozaki 3.834
DTASelect 4.088
Thurlkill 3.554
EMBOSS 3.668
Sillero 3.859
Patrickios 0.248
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.771
Protein with the highest isoelectric point:
>tr|Q0ZNZ6|Q0ZNZ6_9CBAC Uncharacterized protein OS=Neodiprion abietis NPV OX=204507 PE=4 SV=1
MM1 pKa = 7.53 LSNRR5 pKa = 11.84 ITAEE9 pKa = 3.78 TIHH12 pKa = 7.34 RR13 pKa = 11.84 YY14 pKa = 9.26 LCYY17 pKa = 10.45 SNNYY21 pKa = 8.69 RR22 pKa = 11.84 LIVSAVKK29 pKa = 10.12 RR30 pKa = 11.84 SSEE33 pKa = 3.75 ISVSSVTRR41 pKa = 11.84 IIYY44 pKa = 8.82 AQKK47 pKa = 10.9 KK48 pKa = 9.37 LVLFALL54 pKa = 4.49
Molecular weight: 6.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.277
IPC2_protein 9.853
IPC_protein 10.379
Toseland 10.101
ProMoST 9.955
Dawson 10.379
Bjellqvist 10.131
Wikipedia 10.599
Rodwell 10.613
Grimsley 10.482
Solomon 10.423
Lehninger 10.379
Nozaki 10.131
DTASelect 10.116
Thurlkill 10.204
EMBOSS 10.526
Sillero 10.292
Patrickios 10.292
IPC_peptide 10.423
IPC2_peptide 9.151
IPC2.peptide.svr19 8.444
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
93
0
93
24792
51
1136
266.6
30.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.574 ± 0.194
2.686 ± 0.171
6.599 ± 0.212
5.203 ± 0.226
4.852 ± 0.177
2.836 ± 0.173
2.339 ± 0.126
8.704 ± 0.184
7.176 ± 0.301
9.076 ± 0.208
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.424 ± 0.1
8.132 ± 0.208
2.775 ± 0.158
3.562 ± 0.141
4.013 ± 0.194
6.877 ± 0.23
6.954 ± 0.247
6.381 ± 0.203
0.811 ± 0.075
5.026 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here