Streptococcus satellite phage Javan356
Average proteome isoelectric point is 7.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLM3|A0A4D5ZLM3_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan356 OX=2558656 GN=JavanS356_0009 PE=4 SV=1
MM1 pKa = 6.83 FTIEE5 pKa = 4.22 EE6 pKa = 5.06 LINADD11 pKa = 4.55 KK12 pKa = 11.19 EE13 pKa = 4.48 NNTNFADD20 pKa = 3.41 ILINYY25 pKa = 9.02 ISLNDD30 pKa = 3.75 YY31 pKa = 10.3 DD32 pKa = 4.95 KK33 pKa = 11.34 KK34 pKa = 10.82 IAFDD38 pKa = 4.15 LVQKK42 pKa = 10.76 LSEE45 pKa = 3.95 RR46 pKa = 11.84 DD47 pKa = 3.47 KK48 pKa = 11.41 EE49 pKa = 4.26 KK50 pKa = 10.88 EE51 pKa = 3.81
Molecular weight: 6.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.595
IPC2_protein 4.469
IPC_protein 4.304
Toseland 4.139
ProMoST 4.342
Dawson 4.266
Bjellqvist 4.507
Wikipedia 4.164
Rodwell 4.139
Grimsley 4.05
Solomon 4.253
Lehninger 4.215
Nozaki 4.38
DTASelect 4.546
Thurlkill 4.164
EMBOSS 4.177
Sillero 4.418
Patrickios 4.139
IPC_peptide 4.253
IPC2_peptide 4.406
IPC2.peptide.svr19 4.347
Protein with the highest isoelectric point:
>tr|A0A4D5ZQT1|A0A4D5ZQT1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan356 OX=2558656 GN=JavanS356_0011 PE=4 SV=1
MM1 pKa = 7.86 LITSTQAKK9 pKa = 9.74 AIRR12 pKa = 11.84 RR13 pKa = 11.84 KK14 pKa = 9.64 QADD17 pKa = 3.46 KK18 pKa = 11.4 NLTAKK23 pKa = 10.33 RR24 pKa = 11.84 ASEE27 pKa = 4.32 EE28 pKa = 3.83 IGVNPITYY36 pKa = 10.11 RR37 pKa = 11.84 KK38 pKa = 9.32 IRR40 pKa = 11.84 DD41 pKa = 3.51 GGEE44 pKa = 4.09 VKK46 pKa = 10.52 PSIYY50 pKa = 9.91 QKK52 pKa = 11.14 AMQWLAEE59 pKa = 4.48 DD60 pKa = 4.07 YY61 pKa = 11.23
Molecular weight: 6.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.04
IPC2_protein 9.253
IPC_protein 9.253
Toseland 10.014
ProMoST 9.692
Dawson 10.189
Bjellqvist 9.823
Wikipedia 10.335
Rodwell 10.687
Grimsley 10.248
Solomon 10.233
Lehninger 10.204
Nozaki 9.955
DTASelect 9.823
Thurlkill 10.043
EMBOSS 10.394
Sillero 10.087
Patrickios 10.452
IPC_peptide 10.233
IPC2_peptide 8.273
IPC2.peptide.svr19 8.242
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15
0
15
2507
51
388
167.1
19.44
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.388 ± 0.352
0.758 ± 0.145
6.382 ± 0.435
7.938 ± 0.806
4.587 ± 0.392
4.507 ± 0.686
1.715 ± 0.295
6.901 ± 0.503
11.767 ± 0.601
9.533 ± 0.47
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.314 ± 0.353
5.824 ± 0.426
1.795 ± 0.229
4.108 ± 0.273
4.348 ± 0.279
6.103 ± 0.354
6.462 ± 0.672
5.305 ± 0.445
0.758 ± 0.109
4.507 ± 0.413
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here