Microbacterium phage Cinna

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mementomorivirus; unclassified Mementomorivirus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 93 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DDH8|A0A514DDH8_9CAUD Uncharacterized protein OS=Microbacterium phage Cinna OX=2591215 GN=74 PE=4 SV=1
MM1 pKa = 7.67SDD3 pKa = 4.21YY4 pKa = 10.81IRR6 pKa = 11.84LAEE9 pKa = 4.28PPQWSYY15 pKa = 12.1SSPTCSACTVDD26 pKa = 3.96LTHH29 pKa = 7.79DD30 pKa = 3.98GDD32 pKa = 3.52EE33 pKa = 4.42WMCPVCGTTWPSDD46 pKa = 3.72ANDD49 pKa = 3.7GDD51 pKa = 3.83TGNLYY56 pKa = 8.47EE57 pKa = 4.14QWSGEE62 pKa = 4.3SLGDD66 pKa = 3.94APTLDD71 pKa = 3.75EE72 pKa = 4.89SAASDD77 pKa = 2.91AGYY80 pKa = 10.42AYY82 pKa = 10.07EE83 pKa = 3.87RR84 pKa = 11.84AEE86 pKa = 4.15RR87 pKa = 11.84KK88 pKa = 9.4RR89 pKa = 11.84AFLSFGWCEE98 pKa = 3.42HH99 pKa = 5.42GMPGEE104 pKa = 4.29CARR107 pKa = 11.84RR108 pKa = 11.84EE109 pKa = 4.29CPGGTKK115 pKa = 10.47GDD117 pKa = 3.61THH119 pKa = 6.99

Molecular weight: 12.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DDC6|A0A514DDC6_9CAUD Uncharacterized protein OS=Microbacterium phage Cinna OX=2591215 GN=37 PE=4 SV=1
MM1 pKa = 7.63SSGNKK6 pKa = 8.66PVPRR10 pKa = 11.84TMEE13 pKa = 4.37EE14 pKa = 3.5ILRR17 pKa = 11.84DD18 pKa = 3.52TRR20 pKa = 11.84SRR22 pKa = 11.84LLRR25 pKa = 11.84VEE27 pKa = 4.02RR28 pKa = 11.84RR29 pKa = 11.84LSVRR33 pKa = 11.84GGLPARR39 pKa = 11.84LGPNGAQVTDD49 pKa = 3.47WNLATEE55 pKa = 4.47AGFYY59 pKa = 9.64WSEE62 pKa = 3.6AAALNNPVGNVATGIVTVKK81 pKa = 10.59PNGANPRR88 pKa = 11.84VLQEE92 pKa = 3.98VFFPTTTDD100 pKa = 3.13SARR103 pKa = 11.84GRR105 pKa = 11.84SWRR108 pKa = 11.84RR109 pKa = 11.84VLNISTGVWSPWVRR123 pKa = 11.84VGNGPWNGTAAQRR136 pKa = 11.84AASPSQYY143 pKa = 9.96WEE145 pKa = 4.11FWQDD149 pKa = 2.68TDD151 pKa = 4.28GDD153 pKa = 3.57QGLYY157 pKa = 10.29VGNKK161 pKa = 6.81TGGWRR166 pKa = 11.84RR167 pKa = 11.84FSGVDD172 pKa = 3.44SVAAGAWAMNSTSGTVTTVGRR193 pKa = 11.84TVVFTIPTVLEE204 pKa = 4.11TTEE207 pKa = 4.23TLLVTDD213 pKa = 4.57TNTGTGYY220 pKa = 10.96GISAVTAVVRR230 pKa = 11.84NPTNTTLTMRR240 pKa = 11.84HH241 pKa = 5.1MQLGSTVQQSLSIAWQIVQHH261 pKa = 5.94

Molecular weight: 28.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

93

0

93

17753

45

948

190.9

20.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.849 ± 0.44

0.867 ± 0.112

6.382 ± 0.289

6.917 ± 0.365

2.14 ± 0.146

8.759 ± 0.307

2.129 ± 0.201

4.613 ± 0.202

2.405 ± 0.201

7.272 ± 0.409

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.36 ± 0.119

2.557 ± 0.159

6.275 ± 0.247

2.861 ± 0.138

7.638 ± 0.434

4.991 ± 0.276

7.638 ± 0.233

7.041 ± 0.236

1.955 ± 0.157

2.349 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski