Amino acid dipepetide frequency for Microbacterium lacticum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.684AlaAla: 22.684 ± 0.275	0.735AlaCys: 0.735 ± 0.032	8.853AlaAsp: 8.853 ± 0.111	8.383AlaGlu: 8.383 ± 0.121	4.024AlaPhe: 4.024 ± 0.074	12.848AlaGly: 12.848 ± 0.153	2.804AlaHis: 2.804 ± 0.058	5.691AlaIle: 5.691 ± 0.085	2.718AlaLys: 2.718 ± 0.068	14.463AlaLeu: 14.463 ± 0.169	2.598AlaMet: 2.598 ± 0.055	2.175AlaAsn: 2.175 ± 0.059	7.059AlaPro: 7.059 ± 0.113	4.079AlaGln: 4.079 ± 0.081	10.448AlaArg: 10.448 ± 0.143	7.863AlaSer: 7.863 ± 0.106	7.917AlaThr: 7.917 ± 0.11	11.676AlaVal: 11.676 ± 0.16	2.05AlaTrp: 2.05 ± 0.053	2.681AlaTyr: 2.681 ± 0.06	0.0AlaXaa: 0.0 ± 0.0
Cys	0.745CysAla: 0.745 ± 0.031	0.05CysCys: 0.05 ± 0.009	0.289CysAsp: 0.289 ± 0.021	0.232CysGlu: 0.232 ± 0.018	0.145CysPhe: 0.145 ± 0.011	0.559CysGly: 0.559 ± 0.025	0.123CysHis: 0.123 ± 0.012	0.185CysIle: 0.185 ± 0.015	0.067CysLys: 0.067 ± 0.01	0.415CysLeu: 0.415 ± 0.021	0.081CysMet: 0.081 ± 0.011	0.101CysAsn: 0.101 ± 0.012	0.341CysPro: 0.341 ± 0.024	0.143CysGln: 0.143 ± 0.014	0.376CysArg: 0.376 ± 0.027	0.289CysSer: 0.289 ± 0.019	0.321CysThr: 0.321 ± 0.02	0.392CysVal: 0.392 ± 0.02	0.059CysTrp: 0.059 ± 0.008	0.091CysTyr: 0.091 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	10.515AspAla: 10.515 ± 0.125	0.238AspCys: 0.238 ± 0.018	4.712AspAsp: 4.712 ± 0.086	4.57AspGlu: 4.57 ± 0.074	1.591AspPhe: 1.591 ± 0.043	6.027AspGly: 6.027 ± 0.088	1.176AspHis: 1.176 ± 0.044	2.288AspIle: 2.288 ± 0.054	1.115AspLys: 1.115 ± 0.039	6.502AspLeu: 6.502 ± 0.086	0.81AspMet: 0.81 ± 0.027	0.912AspAsn: 0.912 ± 0.037	4.265AspPro: 4.265 ± 0.079	1.601AspGln: 1.601 ± 0.045	4.72AspArg: 4.72 ± 0.077	2.298AspSer: 2.298 ± 0.057	3.012AspThr: 3.012 ± 0.062	5.402AspVal: 5.402 ± 0.086	0.939AspTrp: 0.939 ± 0.032	1.282AspTyr: 1.282 ± 0.043	0.0AspXaa: 0.0 ± 0.0
Glu	7.181GluAla: 7.181 ± 0.111	0.234GluCys: 0.234 ± 0.015	2.574GluAsp: 2.574 ± 0.057	2.715GluGlu: 2.715 ± 0.065	1.599GluPhe: 1.599 ± 0.042	4.042GluGly: 4.042 ± 0.07	1.496GluHis: 1.496 ± 0.048	2.857GluIle: 2.857 ± 0.065	1.438GluLys: 1.438 ± 0.045	6.17GluLeu: 6.17 ± 0.099	0.885GluMet: 0.885 ± 0.032	1.292GluAsn: 1.292 ± 0.038	2.945GluPro: 2.945 ± 0.061	2.215GluGln: 2.215 ± 0.052	5.436GluArg: 5.436 ± 0.092	2.558GluSer: 2.558 ± 0.055	3.089GluThr: 3.089 ± 0.068	4.801GluVal: 4.801 ± 0.076	0.873GluTrp: 0.873 ± 0.031	1.142GluTyr: 1.142 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	4.343PheAla: 4.343 ± 0.077	0.149PheCys: 0.149 ± 0.014	2.296PheAsp: 2.296 ± 0.048	1.616PheGlu: 1.616 ± 0.049	0.984PhePhe: 0.984 ± 0.041	3.162PheGly: 3.162 ± 0.067	0.566PheHis: 0.566 ± 0.026	1.083PheIle: 1.083 ± 0.035	0.457PheLys: 0.457 ± 0.02	2.618PheLeu: 2.618 ± 0.066	0.406PheMet: 0.406 ± 0.023	0.598PheAsn: 0.598 ± 0.024	1.302PhePro: 1.302 ± 0.045	0.687PheGln: 0.687 ± 0.027	1.851PheArg: 1.851 ± 0.045	1.598PheSer: 1.598 ± 0.051	1.976PheThr: 1.976 ± 0.05	2.735PheVal: 2.735 ± 0.059	0.44PheTrp: 0.44 ± 0.023	0.673PheTyr: 0.673 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	11.604GlyAla: 11.604 ± 0.172	0.527GlyCys: 0.527 ± 0.026	5.432GlyAsp: 5.432 ± 0.091	5.077GlyGlu: 5.077 ± 0.076	3.106GlyPhe: 3.106 ± 0.056	7.919GlyGly: 7.919 ± 0.117	1.751GlyHis: 1.751 ± 0.049	4.61GlyIle: 4.61 ± 0.074	2.225GlyLys: 2.225 ± 0.066	8.292GlyLeu: 8.292 ± 0.107	1.87GlyMet: 1.87 ± 0.047	1.511GlyAsn: 1.511 ± 0.046	3.659GlyPro: 3.659 ± 0.072	2.577GlyGln: 2.577 ± 0.05	6.597GlyArg: 6.597 ± 0.098	5.057GlySer: 5.057 ± 0.086	5.476GlyThr: 5.476 ± 0.087	7.885GlyVal: 7.885 ± 0.095	1.611GlyTrp: 1.611 ± 0.05	2.212GlyTyr: 2.212 ± 0.043	0.0GlyXaa: 0.0 ± 0.0
His	2.682HisAla: 2.682 ± 0.053	0.137HisCys: 0.137 ± 0.013	1.399HisAsp: 1.399 ± 0.049	1.14HisGlu: 1.14 ± 0.042	0.495HisPhe: 0.495 ± 0.023	2.01HisGly: 2.01 ± 0.044	0.673HisHis: 0.673 ± 0.028	0.71HisIle: 0.71 ± 0.03	0.328HisLys: 0.328 ± 0.019	2.108HisLeu: 2.108 ± 0.053	0.288HisMet: 0.288 ± 0.017	0.331HisAsn: 0.331 ± 0.018	1.574HisPro: 1.574 ± 0.043	0.482HisGln: 0.482 ± 0.025	1.648HisArg: 1.648 ± 0.046	0.971HisSer: 0.971 ± 0.035	1.105HisThr: 1.105 ± 0.036	1.84HisVal: 1.84 ± 0.053	0.295HisTrp: 0.295 ± 0.019	0.449HisTyr: 0.449 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.887IleAla: 6.887 ± 0.102	0.223IleCys: 0.223 ± 0.015	3.684IleAsp: 3.684 ± 0.066	2.718IleGlu: 2.718 ± 0.066	1.065IlePhe: 1.065 ± 0.036	4.426IleGly: 4.426 ± 0.075	0.827IleHis: 0.827 ± 0.034	1.716IleIle: 1.716 ± 0.047	0.783IleLys: 0.783 ± 0.034	3.514IleLeu: 3.514 ± 0.081	0.662IleMet: 0.662 ± 0.032	0.801IleAsn: 0.801 ± 0.032	2.308IlePro: 2.308 ± 0.054	0.948IleGln: 0.948 ± 0.034	2.7IleArg: 2.7 ± 0.05	2.21IleSer: 2.21 ± 0.054	2.736IleThr: 2.736 ± 0.062	4.704IleVal: 4.704 ± 0.078	0.47IleTrp: 0.47 ± 0.026	0.683IleTyr: 0.683 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	2.585LysAla: 2.585 ± 0.066	0.061LysCys: 0.061 ± 0.009	1.153LysAsp: 1.153 ± 0.04	0.956LysGlu: 0.956 ± 0.035	0.443LysPhe: 0.443 ± 0.022	1.564LysGly: 1.564 ± 0.047	0.418LysHis: 0.418 ± 0.024	0.95LysIle: 0.95 ± 0.033	0.872LysLys: 0.872 ± 0.033	1.927LysLeu: 1.927 ± 0.057	0.377LysMet: 0.377 ± 0.02	0.518LysAsn: 0.518 ± 0.025	1.188LysPro: 1.188 ± 0.047	0.688LysGln: 0.688 ± 0.029	1.609LysArg: 1.609 ± 0.049	1.034LysSer: 1.034 ± 0.038	1.433LysThr: 1.433 ± 0.045	1.76LysVal: 1.76 ± 0.055	0.247LysTrp: 0.247 ± 0.017	0.47LysTyr: 0.47 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	14.43LeuAla: 14.43 ± 0.166	0.494LeuCys: 0.494 ± 0.024	6.849LeuAsp: 6.849 ± 0.103	4.873LeuGlu: 4.873 ± 0.088	2.767LeuPhe: 2.767 ± 0.068	8.77LeuGly: 8.77 ± 0.105	1.911LeuHis: 1.911 ± 0.053	4.368LeuIle: 4.368 ± 0.081	1.716LeuLys: 1.716 ± 0.044	9.611LeuLeu: 9.611 ± 0.143	1.588LeuMet: 1.588 ± 0.049	1.599LeuAsn: 1.599 ± 0.045	5.323LeuPro: 5.323 ± 0.086	2.455LeuGln: 2.455 ± 0.052	7.853LeuArg: 7.853 ± 0.112	5.416LeuSer: 5.416 ± 0.084	6.376LeuThr: 6.376 ± 0.094	9.043LeuVal: 9.043 ± 0.113	1.321LeuTrp: 1.321 ± 0.045	1.713LeuTyr: 1.713 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	2.022MetAla: 2.022 ± 0.049	0.097MetCys: 0.097 ± 0.011	0.839MetAsp: 0.839 ± 0.027	0.53MetGlu: 0.53 ± 0.028	0.507MetPhe: 0.507 ± 0.023	1.242MetGly: 1.242 ± 0.046	0.32MetHis: 0.32 ± 0.019	0.816MetIle: 0.816 ± 0.035	0.393MetLys: 0.393 ± 0.019	1.803MetLeu: 1.803 ± 0.044	0.315MetMet: 0.315 ± 0.02	0.454MetAsn: 0.454 ± 0.022	1.14MetPro: 1.14 ± 0.033	0.481MetGln: 0.481 ± 0.021	1.392MetArg: 1.392 ± 0.042	1.455MetSer: 1.455 ± 0.042	1.626MetThr: 1.626 ± 0.045	1.284MetVal: 1.284 ± 0.037	0.2MetTrp: 0.2 ± 0.015	0.223MetTyr: 0.223 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.418AsnAla: 2.418 ± 0.054	0.102AsnCys: 0.102 ± 0.012	1.105AsnAsp: 1.105 ± 0.036	0.946AsnGlu: 0.946 ± 0.032	0.502AsnPhe: 0.502 ± 0.026	1.7AsnGly: 1.7 ± 0.046	0.353AsnHis: 0.353 ± 0.019	0.754AsnIle: 0.754 ± 0.032	0.383AsnLys: 0.383 ± 0.019	1.87AsnLeu: 1.87 ± 0.043	0.301AsnMet: 0.301 ± 0.019	0.441AsnAsn: 0.441 ± 0.022	1.482AsnPro: 1.482 ± 0.044	0.513AsnGln: 0.513 ± 0.026	1.296AsnArg: 1.296 ± 0.036	0.795AsnSer: 0.795 ± 0.035	1.079AsnThr: 1.079 ± 0.038	1.52AsnVal: 1.52 ± 0.043	0.261AsnTrp: 0.261 ± 0.016	0.421AsnTyr: 0.421 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	7.882ProAla: 7.882 ± 0.106	0.187ProCys: 0.187 ± 0.014	3.964ProAsp: 3.964 ± 0.069	3.711ProGlu: 3.711 ± 0.068	1.586ProPhe: 1.586 ± 0.045	5.125ProGly: 5.125 ± 0.099	1.168ProHis: 1.168 ± 0.043	2.072ProIle: 2.072 ± 0.055	1.009ProLys: 1.009 ± 0.041	4.809ProLeu: 4.809 ± 0.076	0.785ProMet: 0.785 ± 0.03	0.947ProAsn: 0.947 ± 0.033	2.345ProPro: 2.345 ± 0.072	1.514ProGln: 1.514 ± 0.041	3.736ProArg: 3.736 ± 0.072	2.937ProSer: 2.937 ± 0.059	3.373ProThr: 3.373 ± 0.086	4.878ProVal: 4.878 ± 0.081	0.887ProTrp: 0.887 ± 0.036	1.076ProTyr: 1.076 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	3.61GlnAla: 3.61 ± 0.074	0.113GlnCys: 0.113 ± 0.013	1.244GlnAsp: 1.244 ± 0.04	1.239GlnGlu: 1.239 ± 0.036	0.818GlnPhe: 0.818 ± 0.029	2.026GlnGly: 2.026 ± 0.053	0.625GlnHis: 0.625 ± 0.027	1.433GlnIle: 1.433 ± 0.044	0.722GlnLys: 0.722 ± 0.029	3.13GlnLeu: 3.13 ± 0.07	0.489GlnMet: 0.489 ± 0.027	0.608GlnAsn: 0.608 ± 0.029	1.468GlnPro: 1.468 ± 0.042	1.148GlnGln: 1.148 ± 0.045	2.675GlnArg: 2.675 ± 0.067	1.447GlnSer: 1.447 ± 0.047	1.742GlnThr: 1.742 ± 0.042	2.35GlnVal: 2.35 ± 0.048	0.44GlnTrp: 0.44 ± 0.021	0.584GlnTyr: 0.584 ± 0.027	0.0GlnXaa: 0.0 ± 0.0
Arg	10.044ArgAla: 10.044 ± 0.138	0.357ArgCys: 0.357 ± 0.02	4.437ArgAsp: 4.437 ± 0.075	4.65ArgGlu: 4.65 ± 0.086	2.433ArgPhe: 2.433 ± 0.053	6.018ArgGly: 6.018 ± 0.082	1.761ArgHis: 1.761 ± 0.04	3.573ArgIle: 3.573 ± 0.069	1.54ArgLys: 1.54 ± 0.051	7.739ArgLeu: 7.739 ± 0.11	1.695ArgMet: 1.695 ± 0.047	1.338ArgAsn: 1.338 ± 0.042	3.916ArgPro: 3.916 ± 0.08	2.146ArgGln: 2.146 ± 0.056	7.292ArgArg: 7.292 ± 0.159	4.159ArgSer: 4.159 ± 0.065	4.691ArgThr: 4.691 ± 0.076	6.473ArgVal: 6.473 ± 0.095	1.196ArgTrp: 1.196 ± 0.042	1.676ArgTyr: 1.676 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	7.261SerAla: 7.261 ± 0.099	0.287SerCys: 0.287 ± 0.019	3.269SerAsp: 3.269 ± 0.059	2.518SerGlu: 2.518 ± 0.056	1.719SerPhe: 1.719 ± 0.047	5.542SerGly: 5.542 ± 0.09	1.031SerHis: 1.031 ± 0.038	2.374SerIle: 2.374 ± 0.05	1.028SerLys: 1.028 ± 0.04	4.878SerLeu: 4.878 ± 0.078	1.13SerMet: 1.13 ± 0.038	1.03SerAsn: 1.03 ± 0.036	3.013SerPro: 3.013 ± 0.065	1.305SerGln: 1.305 ± 0.039	3.69SerArg: 3.69 ± 0.062	3.133SerSer: 3.133 ± 0.076	3.451SerThr: 3.451 ± 0.062	4.343SerVal: 4.343 ± 0.085	0.84SerTrp: 0.84 ± 0.033	1.095SerTyr: 1.095 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	8.218ThrAla: 8.218 ± 0.119	0.306ThrCys: 0.306 ± 0.021	3.737ThrAsp: 3.737 ± 0.073	3.01ThrGlu: 3.01 ± 0.064	1.784ThrPhe: 1.784 ± 0.048	6.045ThrGly: 6.045 ± 0.089	1.13ThrHis: 1.13 ± 0.035	2.805ThrIle: 2.805 ± 0.064	1.216ThrLys: 1.216 ± 0.04	5.966ThrLeu: 5.966 ± 0.088	0.909ThrMet: 0.909 ± 0.035	1.142ThrAsn: 1.142 ± 0.038	4.253ThrPro: 4.253 ± 0.076	1.516ThrGln: 1.516 ± 0.046	4.06ThrArg: 4.06 ± 0.07	3.25ThrSer: 3.25 ± 0.062	3.949ThrThr: 3.949 ± 0.065	5.836ThrVal: 5.836 ± 0.095	0.848ThrTrp: 0.848 ± 0.03	1.206ThrTyr: 1.206 ± 0.044	0.0ThrXaa: 0.0 ± 0.0
Val	12.356ValAla: 12.356 ± 0.131	0.499ValCys: 0.499 ± 0.023	5.964ValAsp: 5.964 ± 0.081	4.882ValGlu: 4.882 ± 0.086	2.767ValPhe: 2.767 ± 0.06	7.026ValGly: 7.026 ± 0.097	1.814ValHis: 1.814 ± 0.046	4.391ValIle: 4.391 ± 0.077	1.6ValLys: 1.6 ± 0.047	8.821ValLeu: 8.821 ± 0.115	1.382ValMet: 1.382 ± 0.043	1.582ValAsn: 1.582 ± 0.043	4.73ValPro: 4.73 ± 0.082	2.271ValGln: 2.271 ± 0.05	6.435ValArg: 6.435 ± 0.09	4.606ValSer: 4.606 ± 0.082	5.788ValThr: 5.788 ± 0.098	8.747ValVal: 8.747 ± 0.137	1.182ValTrp: 1.182 ± 0.038	1.551ValTyr: 1.551 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	1.649TrpAla: 1.649 ± 0.045	0.107TrpCys: 0.107 ± 0.012	0.752TrpAsp: 0.752 ± 0.036	0.637TrpGlu: 0.637 ± 0.029	0.526TrpPhe: 0.526 ± 0.024	1.016TrpGly: 1.016 ± 0.036	0.374TrpHis: 0.374 ± 0.024	0.702TrpIle: 0.702 ± 0.032	0.272TrpLys: 0.272 ± 0.017	1.722TrpLeu: 1.722 ± 0.05	0.349TrpMet: 0.349 ± 0.019	0.377TrpAsn: 0.377 ± 0.021	0.701TrpPro: 0.701 ± 0.032	0.576TrpGln: 0.576 ± 0.025	1.49TrpArg: 1.49 ± 0.043	0.87TrpSer: 0.87 ± 0.033	0.918TrpThr: 0.918 ± 0.032	1.154TrpVal: 1.154 ± 0.04	0.39TrpTrp: 0.39 ± 0.024	0.286TrpTyr: 0.286 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.634TyrAla: 2.634 ± 0.067	0.103TyrCys: 0.103 ± 0.011	1.423TyrAsp: 1.423 ± 0.043	1.137TyrGlu: 1.137 ± 0.038	0.678TyrPhe: 0.678 ± 0.03	1.929TyrGly: 1.929 ± 0.043	0.345TyrHis: 0.345 ± 0.02	0.689TyrIle: 0.689 ± 0.028	0.37TyrLys: 0.37 ± 0.019	2.115TyrLeu: 2.115 ± 0.047	0.222TyrMet: 0.222 ± 0.016	0.486TyrAsn: 0.486 ± 0.023	0.979TyrPro: 0.979 ± 0.036	0.567TyrGln: 0.567 ± 0.023	1.83TyrArg: 1.83 ± 0.048	0.992TyrSer: 0.992 ± 0.033	1.129TyrThr: 1.129 ± 0.041	1.57TyrVal: 1.57 ± 0.049	0.317TyrTrp: 0.317 ± 0.019	0.425TyrTyr: 0.425 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2765 proteins (875069 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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