Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales; Methanocaldococcaceae; Methanocaldococcus; Methanocaldococcus jannaschii

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1787 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q58573|RELE4_METJA Putative toxin RelE4 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=relE4 PE=3 SV=1
MM1 pKa = 8.33ILMITSTTDD10 pKa = 2.87NLEE13 pKa = 3.55GFKK16 pKa = 10.11IVKK19 pKa = 9.65YY20 pKa = 9.27LGVVIGYY27 pKa = 10.7GDD29 pKa = 4.5DD30 pKa = 3.91PDD32 pKa = 5.48DD33 pKa = 4.72ALEE36 pKa = 4.34DD37 pKa = 4.78LIDD40 pKa = 3.53VAEE43 pKa = 4.57DD44 pKa = 3.05MGANAVIGIRR54 pKa = 11.84ISNEE58 pKa = 3.32LTTEE62 pKa = 4.45IISDD66 pKa = 3.45EE67 pKa = 4.25NYY69 pKa = 10.64AVPEE73 pKa = 4.09LTYY76 pKa = 10.25YY77 pKa = 11.01AYY79 pKa = 8.38GTAVIVEE86 pKa = 4.66KK87 pKa = 10.45IDD89 pKa = 3.21

Molecular weight: 9.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P81227|YE7B_METJA Uncharacterized protein MJ1479.1 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1479.1 PE=4 SV=1
MM1 pKa = 7.68GSNKK5 pKa = 9.71PLGKK9 pKa = 9.82KK10 pKa = 9.59VRR12 pKa = 11.84LAKK15 pKa = 10.33ALKK18 pKa = 8.96QNRR21 pKa = 11.84RR22 pKa = 11.84VPLFVIVKK30 pKa = 7.39TRR32 pKa = 11.84GRR34 pKa = 11.84VRR36 pKa = 11.84FHH38 pKa = 6.65PKK40 pKa = 7.54MRR42 pKa = 11.84YY43 pKa = 5.98WRR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 8.16KK48 pKa = 10.72LKK50 pKa = 10.15AA51 pKa = 3.5

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1787

0

1787

505162

38

2894

282.7

32.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.466 ± 0.066

1.292 ± 0.032

5.521 ± 0.042

8.654 ± 0.071

4.251 ± 0.045

6.332 ± 0.052

1.434 ± 0.023

10.502 ± 0.06

10.39 ± 0.079

9.441 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.294 ± 0.027

5.291 ± 0.065

3.364 ± 0.036

1.454 ± 0.026

3.842 ± 0.043

4.504 ± 0.046

4.055 ± 0.043

6.804 ± 0.053

0.731 ± 0.019

4.377 ± 0.047
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski