Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae; Neurospora; Neurospora crassa

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10267 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A7UW65|A7UW65_NEUCR MFS transporter Fmp42 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=NCU10987 PE=4 SV=2
MM1 pKa = 7.53GWQSSPGRR9 pKa = 11.84LVVAAAGLFGFVNGQAYY26 pKa = 9.94FGTYY30 pKa = 9.23QDD32 pKa = 4.83TITKK36 pKa = 9.99CGSDD40 pKa = 2.91NFIYY44 pKa = 10.34LGCYY48 pKa = 10.55GNFLANAGDD57 pKa = 4.17FFRR60 pKa = 11.84FGPEE64 pKa = 3.79GYY66 pKa = 9.91NAADD70 pKa = 4.12LSLSFPGWDD79 pKa = 4.34PGSDD83 pKa = 3.05WDD85 pKa = 4.33STQTPLDD92 pKa = 3.54CARR95 pKa = 11.84TCRR98 pKa = 11.84AYY100 pKa = 10.25GYY102 pKa = 9.51KK103 pKa = 9.96YY104 pKa = 10.93SAMRR108 pKa = 11.84DD109 pKa = 3.67NNCNCGLQLPAAAVPNSDD127 pKa = 4.48SSACNAQCSGDD138 pKa = 3.69STQTCGGGLNAQVYY152 pKa = 10.02LDD154 pKa = 4.34PSFAAPDD161 pKa = 3.57QVPITPRR168 pKa = 11.84NADD171 pKa = 3.25LGSTYY176 pKa = 10.85KK177 pKa = 10.76HH178 pKa = 6.41LGCYY182 pKa = 9.04SSRR185 pKa = 11.84GFATQDD191 pKa = 3.25DD192 pKa = 4.27VRR194 pKa = 11.84ARR196 pKa = 11.84PLVDD200 pKa = 5.93DD201 pKa = 4.79IEE203 pKa = 4.95TCFEE207 pKa = 3.96TCAGLGYY214 pKa = 9.69PLVFGARR221 pKa = 11.84EE222 pKa = 4.05GAQIRR227 pKa = 11.84CQCGTNFHH235 pKa = 6.6SGDD238 pKa = 3.43YY239 pKa = 10.14RR240 pKa = 11.84IPPSSLPNPGDD251 pKa = 3.91CNALCTADD259 pKa = 4.72DD260 pKa = 4.25TNGDD264 pKa = 4.6CDD266 pKa = 4.64PLTQKK271 pKa = 10.64CCGRR275 pKa = 11.84NEE277 pKa = 4.21HH278 pKa = 6.18YY279 pKa = 9.9PVYY282 pKa = 10.54VSSEE286 pKa = 3.93LQGCYY291 pKa = 8.49VPRR294 pKa = 11.84TPGLKK299 pKa = 9.93QSADD303 pKa = 3.39QAGFACYY310 pKa = 10.02NPPSSLLGPPKK321 pKa = 10.72VLTTSLSSVNQDD333 pKa = 3.13NIIASRR339 pKa = 11.84PALVRR344 pKa = 11.84PLTVTTGTQTWYY356 pKa = 11.39LNGCYY361 pKa = 10.29GNVPAQVLDD370 pKa = 4.22LSAPSGQIISDD381 pKa = 4.38LATGTLEE388 pKa = 3.77EE389 pKa = 4.35CARR392 pKa = 11.84RR393 pKa = 11.84CSSDD397 pKa = 2.92TTFNYY402 pKa = 10.15FGMVNGRR409 pKa = 11.84DD410 pKa = 4.17CYY412 pKa = 10.98CSNTLLTPGQVDD424 pKa = 3.49DD425 pKa = 4.6MGTCRR430 pKa = 11.84VPCQDD435 pKa = 4.47DD436 pKa = 3.53ATRR439 pKa = 11.84SCGGATTPVVYY450 pKa = 10.42SLSQSGVYY458 pKa = 7.61TEE460 pKa = 4.35RR461 pKa = 11.84LANIGAGFPTYY472 pKa = 9.99TCTPSRR478 pKa = 11.84RR479 pKa = 11.84VSNDD483 pKa = 2.14AAAPITCPDD492 pKa = 4.12DD493 pKa = 3.77NGISITTAQDD503 pKa = 2.58KK504 pKa = 9.91MYY506 pKa = 10.59HH507 pKa = 5.4VDD509 pKa = 4.87CGIEE513 pKa = 4.14YY514 pKa = 9.95PDD516 pKa = 4.48GDD518 pKa = 4.59LGTVIVDD525 pKa = 4.0SYY527 pKa = 11.53AEE529 pKa = 4.08CAAACDD535 pKa = 4.26ANIDD539 pKa = 4.05CVGFIYY545 pKa = 10.17QHH547 pKa = 6.48GPANSNGLDD556 pKa = 3.5VPCYY560 pKa = 9.99LKK562 pKa = 10.94RR563 pKa = 11.84EE564 pKa = 4.32GAVTAAVSGDD574 pKa = 3.13TGNWGGRR581 pKa = 11.84FTGFADD587 pKa = 4.56GASSGSDD594 pKa = 2.79SGTGSGSDD602 pKa = 3.55SGSGTDD608 pKa = 3.86PDD610 pKa = 4.18SGSGSGTGTDD620 pKa = 3.88PDD622 pKa = 4.08SGSGSGTGTDD632 pKa = 3.88PDD634 pKa = 4.08SGSGSGTGTDD644 pKa = 3.88PDD646 pKa = 4.08SGSGSGTGTDD656 pKa = 3.88PDD658 pKa = 4.19SGSGTGTGTDD668 pKa = 3.73PDD670 pKa = 4.19SGSGSGTGTDD680 pKa = 3.88PDD682 pKa = 4.19SGSGTGTGTDD692 pKa = 3.73PDD694 pKa = 4.16SGSGSGSGTDD704 pKa = 3.64PDD706 pKa = 4.23SGSGSGDD713 pKa = 3.04GSDD716 pKa = 4.05PGTGSGSGSGNGSGSGTGNGSVVNAIFDD744 pKa = 4.09KK745 pKa = 11.21VFGPAFQGVFSSFGKK760 pKa = 10.46IFDD763 pKa = 4.03NNPFFKK769 pKa = 10.0PKK771 pKa = 10.09KK772 pKa = 8.17PAGSTRR778 pKa = 11.84AVSTGNDD785 pKa = 3.29DD786 pKa = 3.98VQDD789 pKa = 4.51LEE791 pKa = 4.72TLASDD796 pKa = 4.43PSDD799 pKa = 3.57STATRR804 pKa = 11.84SDD806 pKa = 3.26AFIGDD811 pKa = 3.66VLSNLNLGSLMSAVPGLEE829 pKa = 4.01DD830 pKa = 3.49VLGGQDD836 pKa = 4.37LGSLIPGNIRR846 pKa = 11.84APDD849 pKa = 4.0PATPPMSANLDD860 pKa = 3.73GLASLASTVLAALNTLPSDD879 pKa = 3.55IAAPGPVDD887 pKa = 3.66TDD889 pKa = 3.72SLITLASAITDD900 pKa = 3.98ALTTAADD907 pKa = 3.98GSGPGVINGKK917 pKa = 9.9DD918 pKa = 3.21FAGILNSVFDD928 pKa = 4.77SINGIGSIFPKK939 pKa = 10.44KK940 pKa = 9.12STSSSAQPSMTVAPTQSLRR959 pKa = 11.84ARR961 pKa = 11.84DD962 pKa = 3.62VAHH965 pKa = 7.28IGPHH969 pKa = 5.17PTGVLPPGNGPAEE982 pKa = 4.28VCDD985 pKa = 3.64IATLALPANIFAPTPPPDD1003 pKa = 3.9AGRR1006 pKa = 11.84CIPIIGYY1013 pKa = 8.81WYY1015 pKa = 10.55VGEE1018 pKa = 4.35TTLLPCSEE1026 pKa = 4.89GYY1028 pKa = 10.26GAGGPQEE1035 pKa = 4.42TGSPQAPGFSGGPGGHH1051 pKa = 7.3PPPPPPAGPPPAVVIPVAGSIVLTQEE1077 pKa = 4.09QLDD1080 pKa = 3.94HH1081 pKa = 7.38LLEE1084 pKa = 4.32STNIEE1089 pKa = 4.25LEE1091 pKa = 4.13WTSEE1095 pKa = 3.99GEE1097 pKa = 4.05RR1098 pKa = 11.84VLNIPGILDD1107 pKa = 3.47MSDD1110 pKa = 2.91SHH1112 pKa = 8.75SDD1114 pKa = 3.41TFRR1117 pKa = 11.84LLLDD1121 pKa = 3.48QSSDD1125 pKa = 3.76SLSHH1129 pKa = 5.76STNQFEE1135 pKa = 4.41IPISGEE1141 pKa = 3.8VGLTQSQLDD1150 pKa = 3.94SLLQDD1155 pKa = 3.26STLDD1159 pKa = 3.6IFWNSQGAKK1168 pKa = 8.95EE1169 pKa = 3.93LNIPGVINYY1178 pKa = 9.53SSSRR1182 pKa = 11.84NDD1184 pKa = 3.27TFSLRR1189 pKa = 11.84ISSSNHH1195 pKa = 4.95HH1196 pKa = 5.55TTSTASIPGPTEE1208 pKa = 3.71SSTPPAAGGEE1218 pKa = 4.41TPDD1221 pKa = 4.66GSGADD1226 pKa = 3.66EE1227 pKa = 5.0DD1228 pKa = 4.36GGYY1231 pKa = 10.93DD1232 pKa = 4.09DD1233 pKa = 6.27VEE1235 pKa = 5.98AEE1237 pKa = 4.26LAALDD1242 pKa = 4.06ALIASFGDD1250 pKa = 2.93IDD1252 pKa = 5.12FDD1254 pKa = 5.27VGDD1257 pKa = 4.94LDD1259 pKa = 4.65IPDD1262 pKa = 4.04IDD1264 pKa = 4.6VEE1266 pKa = 4.36GLDD1269 pKa = 4.13FPTVPSIDD1277 pKa = 3.79TDD1279 pKa = 3.96DD1280 pKa = 5.53LDD1282 pKa = 4.69VDD1284 pKa = 3.74VDD1286 pKa = 4.02DD1287 pKa = 4.94VDD1289 pKa = 3.8VSRR1292 pKa = 11.84VEE1294 pKa = 4.51APEE1297 pKa = 4.17SPEE1300 pKa = 4.03VPDD1303 pKa = 3.57VDD1305 pKa = 5.97AIPAPTIDD1313 pKa = 3.13VDD1315 pKa = 4.38GEE1317 pKa = 4.57VQVGLDD1323 pKa = 4.14DD1324 pKa = 4.12EE1325 pKa = 5.38SGVPVVDD1332 pKa = 4.39PVDD1335 pKa = 3.44EE1336 pKa = 4.28ATT1338 pKa = 4.18

Molecular weight: 136.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5IKN2|V5IKN2_NEUCR Uncharacterized protein OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=NCU17087 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.09WRR6 pKa = 11.84KK7 pKa = 8.87KK8 pKa = 8.73RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.94RR15 pKa = 11.84KK16 pKa = 7.8RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.6MRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 11.11

Molecular weight: 3.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9759

508

10267

5305852

24

10739

516.8

56.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.676 ± 0.023

1.112 ± 0.009

5.642 ± 0.018

6.486 ± 0.027

3.389 ± 0.014

7.144 ± 0.024

2.457 ± 0.01

4.43 ± 0.013

5.076 ± 0.025

8.345 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.009

3.711 ± 0.016

6.577 ± 0.034

4.279 ± 0.023

6.148 ± 0.02

8.36 ± 0.031

6.138 ± 0.017

5.969 ± 0.018

1.344 ± 0.009

2.564 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski