Proteome-pI 2.0 - Proteome Isoelectric Point Database

Streptomyces pristinaespiralis (strain ATCC 25486 / DSM 40338 / CBS 914.69 / JCM 4507 / NBRC 13074 / NRRL 2958 / 5647)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces pristinaespiralis

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 6866 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|B5H8Q9|B5H8Q9_STRE2 Uncharacterized protein OS=Streptomyces pristinaespiralis (strain ATCC 25486 / DSM 40338 / CBS 914.69 / JCM 4507 / NBRC 13074 / NRRL 2958 / 5647) OX=457429 GN=SSDG_01631 PE=4 SV=1

MM1 pKa = 7.17TVQHH5 pKa = 6.14EE6 pKa = 4.66VPTDD10 pKa = 3.42GATDD14 pKa = 3.67ALEE17 pKa = 3.87VWIDD21 pKa = 3.4QDD23 pKa = 3.99LCTGDD28 pKa = 5.01GICAQYY34 pKa = 10.9APEE37 pKa = 4.23VFEE40 pKa = 5.9LDD42 pKa = 3.05IDD44 pKa = 3.63GLAYY48 pKa = 10.42VKK50 pKa = 10.8SADD53 pKa = 4.84DD54 pKa = 4.41EE55 pKa = 4.67LLQAPGATTPVPLPLLNDD73 pKa = 3.56VVDD76 pKa = 4.2SAKK79 pKa = 10.28EE80 pKa = 3.92CPGDD84 pKa = 4.18CIHH87 pKa = 6.58VRR89 pKa = 11.84RR90 pKa = 11.84VSDD93 pKa = 3.44NVEE96 pKa = 4.07VYY98 pKa = 10.91GPDD101 pKa = 3.4AEE103 pKa = 4.31

Molecular weight: 11.03 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|B5H550|B5H550_STRE2 ECF sigma factor OS=Streptomyces pristinaespiralis (strain ATCC 25486 / DSM 40338 / CBS 914.69 / JCM 4507 / NBRC 13074 / NRRL 2958 / 5647) OX=457429 GN=SSDG_00276 PE=3 SV=1

MM1 pKa = 7.46TFPHH5 pKa = 7.14PGPHH9 pKa = 6.08VGLAGVRR16 pKa = 11.84GSGRR20 pKa = 11.84PGPHH24 pKa = 6.75PNATTRR30 pKa = 11.84FSTLRR35 pKa = 11.84IRR37 pKa = 11.84RR38 pKa = 11.84PRR40 pKa = 11.84TTLRR44 pKa = 11.84TALLRR49 pKa = 11.84GSGTPPGSPSTVRR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84LKK67 pKa = 10.57

Molecular weight: 7.26 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
6866	0	6866	2152991	25	3624	313.6	33.63

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
13.265 ± 0.041	0.827 ± 0.008	6.016 ± 0.022	5.787 ± 0.028	2.787 ± 0.018	9.616 ± 0.027	2.318 ± 0.015	3.202 ± 0.019	2.248 ± 0.029	10.028 ± 0.035

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
1.858 ± 0.012	1.815 ± 0.016	6.09 ± 0.029	2.734 ± 0.015	8.167 ± 0.037	5.118 ± 0.021	6.106 ± 0.028	8.409 ± 0.029	1.519 ± 0.011	2.087 ± 0.017

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski