Amino acid dipepetide frequency for Lysobacter sp. Root96

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.354AlaAla: 20.354 ± 0.21	1.229AlaCys: 1.229 ± 0.032	7.485AlaAsp: 7.485 ± 0.089	7.038AlaGlu: 7.038 ± 0.119	4.158AlaPhe: 4.158 ± 0.063	11.278AlaGly: 11.278 ± 0.108	2.635AlaHis: 2.635 ± 0.05	5.722AlaIle: 5.722 ± 0.063	3.617AlaLys: 3.617 ± 0.081	15.887AlaLeu: 15.887 ± 0.168	3.314AlaMet: 3.314 ± 0.056	2.883AlaAsn: 2.883 ± 0.066	7.226AlaPro: 7.226 ± 0.099	5.788AlaGln: 5.788 ± 0.08	10.055AlaArg: 10.055 ± 0.119	6.64AlaSer: 6.64 ± 0.087	6.343AlaThr: 6.343 ± 0.107	8.998AlaVal: 8.998 ± 0.089	2.001AlaTrp: 2.001 ± 0.051	2.848AlaTyr: 2.848 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	1.13CysAla: 1.13 ± 0.034	0.101CysCys: 0.101 ± 0.01	0.488CysAsp: 0.488 ± 0.021	0.484CysGlu: 0.484 ± 0.022	0.245CysPhe: 0.245 ± 0.014	0.941CysGly: 0.941 ± 0.036	0.193CysHis: 0.193 ± 0.012	0.328CysIle: 0.328 ± 0.016	0.208CysLys: 0.208 ± 0.016	0.766CysLeu: 0.766 ± 0.026	0.133CysMet: 0.133 ± 0.009	0.208CysAsn: 0.208 ± 0.013	0.422CysPro: 0.422 ± 0.022	0.232CysGln: 0.232 ± 0.014	0.563CysArg: 0.563 ± 0.026	0.472CysSer: 0.472 ± 0.02	0.418CysThr: 0.418 ± 0.023	0.667CysVal: 0.667 ± 0.029	0.128CysTrp: 0.128 ± 0.01	0.214CysTyr: 0.214 ± 0.013	0.001CysXaa: 0.001 ± 0.001
Asp	8.695AspAla: 8.695 ± 0.099	0.471AspCys: 0.471 ± 0.021	3.479AspAsp: 3.479 ± 0.062	3.528AspGlu: 3.528 ± 0.061	2.034AspPhe: 2.034 ± 0.038	6.113AspGly: 6.113 ± 0.073	1.161AspHis: 1.161 ± 0.034	2.216AspIle: 2.216 ± 0.041	1.522AspLys: 1.522 ± 0.04	5.741AspLeu: 5.741 ± 0.079	1.007AspMet: 1.007 ± 0.033	1.336AspAsn: 1.336 ± 0.035	3.647AspPro: 3.647 ± 0.055	1.679AspGln: 1.679 ± 0.038	4.267AspArg: 4.267 ± 0.066	2.743AspSer: 2.743 ± 0.047	2.724AspThr: 2.724 ± 0.054	3.904AspVal: 3.904 ± 0.063	1.177AspTrp: 1.177 ± 0.03	1.861AspTyr: 1.861 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	6.956GluAla: 6.956 ± 0.093	0.352GluCys: 0.352 ± 0.017	2.529GluAsp: 2.529 ± 0.05	2.26GluGlu: 2.26 ± 0.051	1.842GluPhe: 1.842 ± 0.041	3.848GluGly: 3.848 ± 0.063	1.439GluHis: 1.439 ± 0.03	2.46GluIle: 2.46 ± 0.047	1.361GluLys: 1.361 ± 0.037	6.292GluLeu: 6.292 ± 0.082	1.032GluMet: 1.032 ± 0.029	1.172GluAsn: 1.172 ± 0.035	2.655GluPro: 2.655 ± 0.048	2.414GluGln: 2.414 ± 0.055	5.805GluArg: 5.805 ± 0.099	2.578GluSer: 2.578 ± 0.044	2.635GluThr: 2.635 ± 0.044	3.68GluVal: 3.68 ± 0.058	0.712GluTrp: 0.712 ± 0.026	1.148GluTyr: 1.148 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	4.45PheAla: 4.45 ± 0.068	0.297PheCys: 0.297 ± 0.016	2.675PheAsp: 2.675 ± 0.049	2.019PheGlu: 2.019 ± 0.042	1.109PhePhe: 1.109 ± 0.033	3.369PheGly: 3.369 ± 0.053	0.683PheHis: 0.683 ± 0.025	1.051PheIle: 1.051 ± 0.027	0.892PheLys: 0.892 ± 0.027	2.902PheLeu: 2.902 ± 0.064	0.601PheMet: 0.601 ± 0.024	1.043PheAsn: 1.043 ± 0.033	1.36PhePro: 1.36 ± 0.031	0.937PheGln: 0.937 ± 0.028	2.151PheArg: 2.151 ± 0.038	1.865PheSer: 1.865 ± 0.043	1.492PheThr: 1.492 ± 0.043	2.714PheVal: 2.714 ± 0.047	0.473PheTrp: 0.473 ± 0.02	0.824PheTyr: 0.824 ± 0.029	0.001PheXaa: 0.001 ± 0.001
Gly	10.051GlyAla: 10.051 ± 0.084	0.798GlyCys: 0.798 ± 0.024	5.036GlyAsp: 5.036 ± 0.058	4.941GlyGlu: 4.941 ± 0.06	3.282GlyPhe: 3.282 ± 0.055	7.588GlyGly: 7.588 ± 0.104	1.938GlyHis: 1.938 ± 0.045	3.646GlyIle: 3.646 ± 0.059	2.904GlyLys: 2.904 ± 0.06	8.921GlyLeu: 8.921 ± 0.104	1.981GlyMet: 1.981 ± 0.043	2.447GlyAsn: 2.447 ± 0.059	3.198GlyPro: 3.198 ± 0.059	3.079GlyGln: 3.079 ± 0.056	6.645GlyArg: 6.645 ± 0.09	4.801GlySer: 4.801 ± 0.088	4.209GlyThr: 4.209 ± 0.089	6.4GlyVal: 6.4 ± 0.077	1.629GlyTrp: 1.629 ± 0.039	2.795GlyTyr: 2.795 ± 0.054	0.002GlyXaa: 0.002 ± 0.001
His	2.858HisAla: 2.858 ± 0.054	0.251HisCys: 0.251 ± 0.014	1.303HisAsp: 1.303 ± 0.031	1.148HisGlu: 1.148 ± 0.036	0.733HisPhe: 0.733 ± 0.028	2.248HisGly: 2.248 ± 0.047	0.556HisHis: 0.556 ± 0.023	0.724HisIle: 0.724 ± 0.026	0.445HisLys: 0.445 ± 0.017	2.032HisLeu: 2.032 ± 0.047	0.383HisMet: 0.383 ± 0.016	0.466HisAsn: 0.466 ± 0.02	1.404HisPro: 1.404 ± 0.035	0.58HisGln: 0.58 ± 0.021	1.651HisArg: 1.651 ± 0.039	0.976HisSer: 0.976 ± 0.031	0.877HisThr: 0.877 ± 0.029	1.381HisVal: 1.381 ± 0.037	0.404HisTrp: 0.404 ± 0.019	0.681HisTyr: 0.681 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	6.917IleAla: 6.917 ± 0.076	0.37IleCys: 0.37 ± 0.019	3.372IleAsp: 3.372 ± 0.051	3.194IleGlu: 3.194 ± 0.051	1.029IlePhe: 1.029 ± 0.033	4.416IleGly: 4.416 ± 0.056	0.73IleHis: 0.73 ± 0.026	1.038IleIle: 1.038 ± 0.032	1.117IleLys: 1.117 ± 0.033	2.801IleLeu: 2.801 ± 0.056	0.418IleMet: 0.418 ± 0.017	1.195IleAsn: 1.195 ± 0.03	1.943IlePro: 1.943 ± 0.042	1.1IleGln: 1.1 ± 0.032	2.552IleArg: 2.552 ± 0.046	1.972IleSer: 1.972 ± 0.047	1.922IleThr: 1.922 ± 0.052	3.472IleVal: 3.472 ± 0.05	0.457IleTrp: 0.457 ± 0.021	0.859IleTyr: 0.859 ± 0.028	0.0IleXaa: 0.0 ± 0.0
Lys	3.232LysAla: 3.232 ± 0.063	0.136LysCys: 0.136 ± 0.013	1.462LysAsp: 1.462 ± 0.037	1.086LysGlu: 1.086 ± 0.034	0.808LysPhe: 0.808 ± 0.026	1.913LysGly: 1.913 ± 0.048	0.601LysHis: 0.601 ± 0.024	1.247LysIle: 1.247 ± 0.037	0.964LysLys: 0.964 ± 0.042	3.164LysLeu: 3.164 ± 0.052	0.586LysMet: 0.586 ± 0.022	0.683LysAsn: 0.683 ± 0.023	2.04LysPro: 2.04 ± 0.052	1.203LysGln: 1.203 ± 0.037	2.215LysArg: 2.215 ± 0.044	1.419LysSer: 1.419 ± 0.034	1.549LysThr: 1.549 ± 0.041	1.944LysVal: 1.944 ± 0.037	0.307LysTrp: 0.307 ± 0.016	0.642LysTyr: 0.642 ± 0.025	0.001LysXaa: 0.001 ± 0.001
Leu	15.198LeuAla: 15.198 ± 0.154	0.926LeuCys: 0.926 ± 0.028	6.778LeuAsp: 6.778 ± 0.087	5.014LeuGlu: 5.014 ± 0.069	3.283LeuPhe: 3.283 ± 0.055	8.696LeuGly: 8.696 ± 0.092	2.309LeuHis: 2.309 ± 0.047	4.443LeuIle: 4.443 ± 0.074	2.967LeuLys: 2.967 ± 0.05	11.467LeuLeu: 11.467 ± 0.158	2.03LeuMet: 2.03 ± 0.044	2.456LeuAsn: 2.456 ± 0.049	6.402LeuPro: 6.402 ± 0.084	3.794LeuGln: 3.794 ± 0.067	9.253LeuArg: 9.253 ± 0.119	6.427LeuSer: 6.427 ± 0.079	4.815LeuThr: 4.815 ± 0.064	7.101LeuVal: 7.101 ± 0.086	1.469LeuTrp: 1.469 ± 0.043	2.259LeuTyr: 2.259 ± 0.048	0.0LeuXaa: 0.0 ± 0.0
Met	2.485MetAla: 2.485 ± 0.048	0.138MetCys: 0.138 ± 0.01	0.965MetAsp: 0.965 ± 0.031	0.802MetGlu: 0.802 ± 0.022	0.584MetPhe: 0.584 ± 0.021	1.367MetGly: 1.367 ± 0.038	0.426MetHis: 0.426 ± 0.019	0.877MetIle: 0.877 ± 0.027	0.762MetLys: 0.762 ± 0.029	2.272MetLeu: 2.272 ± 0.046	0.418MetMet: 0.418 ± 0.018	0.756MetAsn: 0.756 ± 0.019	1.332MetPro: 1.332 ± 0.035	0.851MetGln: 0.851 ± 0.024	1.724MetArg: 1.724 ± 0.036	1.486MetSer: 1.486 ± 0.037	1.271MetThr: 1.271 ± 0.031	1.295MetVal: 1.295 ± 0.039	0.186MetTrp: 0.186 ± 0.014	0.335MetTyr: 0.335 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.432AsnAla: 3.432 ± 0.06	0.221AsnCys: 0.221 ± 0.013	1.488AsnAsp: 1.488 ± 0.041	1.198AsnGlu: 1.198 ± 0.034	0.903AsnPhe: 0.903 ± 0.03	2.569AsnGly: 2.569 ± 0.064	0.47AsnHis: 0.47 ± 0.019	0.961AsnIle: 0.961 ± 0.028	0.59AsnLys: 0.59 ± 0.026	2.472AsnLeu: 2.472 ± 0.045	0.382AsnMet: 0.382 ± 0.02	0.713AsnAsn: 0.713 ± 0.035	1.757AsnPro: 1.757 ± 0.043	0.806AsnGln: 0.806 ± 0.03	1.763AsnArg: 1.763 ± 0.039	1.166AsnSer: 1.166 ± 0.041	1.315AsnThr: 1.315 ± 0.039	1.834AsnVal: 1.834 ± 0.052	0.4AsnTrp: 0.4 ± 0.018	0.697AsnTyr: 0.697 ± 0.028	0.001AsnXaa: 0.001 ± 0.001
Pro	7.677ProAla: 7.677 ± 0.115	0.333ProCys: 0.333 ± 0.017	3.596ProAsp: 3.596 ± 0.057	3.31ProGlu: 3.31 ± 0.055	1.581ProPhe: 1.581 ± 0.038	4.852ProGly: 4.852 ± 0.065	1.033ProHis: 1.033 ± 0.03	2.057ProIle: 2.057 ± 0.039	1.498ProLys: 1.498 ± 0.04	5.255ProLeu: 5.255 ± 0.075	1.28ProMet: 1.28 ± 0.032	1.371ProAsn: 1.371 ± 0.038	2.989ProPro: 2.989 ± 0.071	2.173ProGln: 2.173 ± 0.05	3.499ProArg: 3.499 ± 0.068	2.875ProSer: 2.875 ± 0.053	2.608ProThr: 2.608 ± 0.056	4.02ProVal: 4.02 ± 0.061	0.814ProTrp: 0.814 ± 0.025	1.268ProTyr: 1.268 ± 0.039	0.001ProXaa: 0.001 ± 0.001
Gln	5.308GlnAla: 5.308 ± 0.068	0.246GlnCys: 0.246 ± 0.013	1.638GlnAsp: 1.638 ± 0.04	1.316GlnGlu: 1.316 ± 0.038	1.124GlnPhe: 1.124 ± 0.032	2.777GlnGly: 2.777 ± 0.047	0.767GlnHis: 0.767 ± 0.027	1.594GlnIle: 1.594 ± 0.037	0.819GlnLys: 0.819 ± 0.025	4.011GlnLeu: 4.011 ± 0.065	0.702GlnMet: 0.702 ± 0.027	0.79GlnAsn: 0.79 ± 0.027	2.18GlnPro: 2.18 ± 0.044	1.596GlnGln: 1.596 ± 0.042	3.841GlnArg: 3.841 ± 0.062	1.809GlnSer: 1.809 ± 0.042	1.733GlnThr: 1.733 ± 0.039	2.74GlnVal: 2.74 ± 0.047	0.623GlnTrp: 0.623 ± 0.024	0.792GlnTyr: 0.792 ± 0.027	0.003GlnXaa: 0.003 ± 0.002
Arg	9.318ArgAla: 9.318 ± 0.113	0.617ArgCys: 0.617 ± 0.024	4.857ArgAsp: 4.857 ± 0.063	4.979ArgGlu: 4.979 ± 0.074	2.784ArgPhe: 2.784 ± 0.051	5.628ArgGly: 5.628 ± 0.073	1.791ArgHis: 1.791 ± 0.045	3.883ArgIle: 3.883 ± 0.065	2.133ArgLys: 2.133 ± 0.047	8.754ArgLeu: 8.754 ± 0.115	1.931ArgMet: 1.931 ± 0.038	2.042ArgAsn: 2.042 ± 0.042	3.654ArgPro: 3.654 ± 0.054	2.75ArgGln: 2.75 ± 0.053	6.405ArgArg: 6.405 ± 0.089	4.085ArgSer: 4.085 ± 0.057	3.17ArgThr: 3.17 ± 0.046	5.629ArgVal: 5.629 ± 0.07	1.489ArgTrp: 1.489 ± 0.04	2.68ArgTyr: 2.68 ± 0.051	0.001ArgXaa: 0.001 ± 0.001
Ser	6.753SerAla: 6.753 ± 0.086	0.425SerCys: 0.425 ± 0.025	3.031SerAsp: 3.031 ± 0.047	2.731SerGlu: 2.731 ± 0.049	1.931SerPhe: 1.931 ± 0.046	5.35SerGly: 5.35 ± 0.084	1.125SerHis: 1.125 ± 0.035	2.065SerIle: 2.065 ± 0.043	1.473SerLys: 1.473 ± 0.038	5.604SerLeu: 5.604 ± 0.08	1.066SerMet: 1.066 ± 0.029	1.47SerAsn: 1.47 ± 0.044	2.711SerPro: 2.711 ± 0.048	1.87SerGln: 1.87 ± 0.042	3.606SerArg: 3.606 ± 0.051	2.77SerSer: 2.77 ± 0.063	2.689SerThr: 2.689 ± 0.058	3.81SerVal: 3.81 ± 0.061	0.795SerTrp: 0.795 ± 0.024	1.418SerTyr: 1.418 ± 0.043	0.0SerXaa: 0.0 ± 0.0
Thr	6.118ThrAla: 6.118 ± 0.097	0.387ThrCys: 0.387 ± 0.021	2.555ThrAsp: 2.555 ± 0.049	2.049ThrGlu: 2.049 ± 0.04	1.428ThrPhe: 1.428 ± 0.042	4.349ThrGly: 4.349 ± 0.069	1.008ThrHis: 1.008 ± 0.032	1.994ThrIle: 1.994 ± 0.053	0.985ThrLys: 0.985 ± 0.031	5.818ThrLeu: 5.818 ± 0.07	0.875ThrMet: 0.875 ± 0.026	1.003ThrAsn: 1.003 ± 0.039	3.426ThrPro: 3.426 ± 0.058	1.668ThrGln: 1.668 ± 0.041	3.42ThrArg: 3.42 ± 0.057	2.277ThrSer: 2.277 ± 0.051	2.476ThrThr: 2.476 ± 0.054	4.076ThrVal: 4.076 ± 0.073	0.71ThrTrp: 0.71 ± 0.024	1.255ThrTyr: 1.255 ± 0.048	0.001ThrXaa: 0.001 ± 0.001
Val	9.891ValAla: 9.891 ± 0.081	0.679ValCys: 0.679 ± 0.026	4.253ValAsp: 4.253 ± 0.062	4.262ValGlu: 4.262 ± 0.06	2.52ValPhe: 2.52 ± 0.046	5.649ValGly: 5.649 ± 0.085	1.44ValHis: 1.44 ± 0.036	3.112ValIle: 3.112 ± 0.049	1.881ValLys: 1.881 ± 0.042	7.939ValLeu: 7.939 ± 0.084	1.396ValMet: 1.396 ± 0.036	1.887ValAsn: 1.887 ± 0.048	3.741ValPro: 3.741 ± 0.052	2.295ValGln: 2.295 ± 0.047	5.357ValArg: 5.357 ± 0.066	4.04ValSer: 4.04 ± 0.067	3.484ValThr: 3.484 ± 0.065	5.721ValVal: 5.721 ± 0.088	0.923ValTrp: 0.923 ± 0.025	1.716ValTyr: 1.716 ± 0.041	0.002ValXaa: 0.002 ± 0.001
Trp	1.378TrpAla: 1.378 ± 0.041	0.13TrpCys: 0.13 ± 0.01	0.69TrpAsp: 0.69 ± 0.027	0.539TrpGlu: 0.539 ± 0.021	0.592TrpPhe: 0.592 ± 0.026	0.961TrpGly: 0.961 ± 0.029	0.334TrpHis: 0.334 ± 0.018	0.714TrpIle: 0.714 ± 0.023	0.453TrpLys: 0.453 ± 0.021	2.346TrpLeu: 2.346 ± 0.05	0.4TrpMet: 0.4 ± 0.019	0.521TrpAsn: 0.521 ± 0.021	0.773TrpPro: 0.773 ± 0.025	0.722TrpGln: 0.722 ± 0.023	1.526TrpArg: 1.526 ± 0.039	0.915TrpSer: 0.915 ± 0.026	0.824TrpThr: 0.824 ± 0.033	0.96TrpVal: 0.96 ± 0.03	0.34TrpTrp: 0.34 ± 0.018	0.365TrpTyr: 0.365 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.299TyrAla: 3.299 ± 0.058	0.231TyrCys: 0.231 ± 0.013	1.626TyrAsp: 1.626 ± 0.055	1.273TyrGlu: 1.273 ± 0.037	0.939TyrPhe: 0.939 ± 0.029	2.375TyrGly: 2.375 ± 0.052	0.467TyrHis: 0.467 ± 0.019	0.742TyrIle: 0.742 ± 0.026	0.61TyrLys: 0.61 ± 0.023	2.645TyrLeu: 2.645 ± 0.051	0.396TyrMet: 0.396 ± 0.02	0.696TyrAsn: 0.696 ± 0.032	1.229TyrPro: 1.229 ± 0.033	0.869TyrGln: 0.869 ± 0.03	2.28TyrArg: 2.28 ± 0.043	1.374TyrSer: 1.374 ± 0.037	1.32TyrThr: 1.32 ± 0.047	1.842TyrVal: 1.842 ± 0.039	0.448TyrTrp: 0.448 ± 0.02	0.754TyrTyr: 0.754 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.001XaaCys: 0.001 ± 0.001	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.002XaaGly: 0.002 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.003XaaArg: 0.003 ± 0.002	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.001XaaTyr: 0.001 ± 0.001	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3827 proteins (1228680 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---