Thiafora orthonairovirus
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M5KMK1|A0A0M5KMK1_9VIRU Glycoprotein G2 OS=Thiafora orthonairovirus OX=1980529 PE=4 SV=1
MM1 pKa = 5.87 EE2 pKa = 4.79 TKK4 pKa = 9.65 WVVAVALVMQLYY16 pKa = 9.93 QLLSLTVSSTSTTTPTVEE34 pKa = 4.29 STTSPNTSSATNATTGASGPTAQGISSSTSNQSVKK69 pKa = 10.8 SVDD72 pKa = 3.66 QIVSYY77 pKa = 10.42 AANIWDD83 pKa = 3.93 STLEE87 pKa = 3.86 ALFTKK92 pKa = 9.61 DD93 pKa = 3.06 TSCNKK98 pKa = 9.48 NLSWCRR104 pKa = 11.84 LEE106 pKa = 4.32 VTDD109 pKa = 3.83 THH111 pKa = 6.91 GLTPYY116 pKa = 10.33 VKK118 pKa = 10.15 HH119 pKa = 6.68 LYY121 pKa = 9.83 NLSNSKK127 pKa = 10.96 YY128 pKa = 9.18 NTFCQSKK135 pKa = 9.55 KK136 pKa = 10.79 GNFGFIKK143 pKa = 10.32 KK144 pKa = 9.77 EE145 pKa = 3.7 KK146 pKa = 9.22 FTFDD150 pKa = 3.1 VTTGPEE156 pKa = 3.65 RR157 pKa = 11.84 MLLKK161 pKa = 10.47 DD162 pKa = 3.43 IQCANVIYY170 pKa = 10.78 DD171 pKa = 3.83 GVTKK175 pKa = 10.43 DD176 pKa = 3.28 GYY178 pKa = 10.81 LLHH181 pKa = 7.22 VLFGGRR187 pKa = 11.84 RR188 pKa = 11.84 VFFYY192 pKa = 10.35 SCRR195 pKa = 11.84 YY196 pKa = 8.13 TIITKK201 pKa = 9.43 NCRR204 pKa = 11.84 IRR206 pKa = 11.84 ASHH209 pKa = 7.11 DD210 pKa = 3.39 APVPLPGYY218 pKa = 9.73 GNWTTAMYY226 pKa = 10.7 SVFLEE231 pKa = 4.14 HH232 pKa = 7.19 RR233 pKa = 11.84 SAAEE237 pKa = 3.58 TCKK240 pKa = 10.36 IYY242 pKa = 10.41 FPCLNKK248 pKa = 10.44 GKK250 pKa = 9.32 PLGNGGFKK258 pKa = 10.4 IKK260 pKa = 10.69 GFFVTGLTKK269 pKa = 10.56 PEE271 pKa = 3.81 GTGRR275 pKa = 11.84 KK276 pKa = 8.98 LLTAADD282 pKa = 4.26 PEE284 pKa = 4.29 PDD286 pKa = 3.96 EE287 pKa = 5.36 DD288 pKa = 4.5 CGSASHH294 pKa = 7.09 LKK296 pKa = 10.49 QITNHH301 pKa = 6.18 HH302 pKa = 6.63 LVTDD306 pKa = 4.96 FKK308 pKa = 11.23 DD309 pKa = 3.99 GPGDD313 pKa = 3.78 VISICNGSTFFHH325 pKa = 6.23 GRR327 pKa = 11.84 MPDD330 pKa = 3.05 GLGCHH335 pKa = 7.0 SIRR338 pKa = 11.84 SIKK341 pKa = 9.93 VSHH344 pKa = 6.69 HH345 pKa = 6.7 CGHH348 pKa = 7.13 HH349 pKa = 4.55 STKK352 pKa = 10.05 CTVEE356 pKa = 4.31 PDD358 pKa = 4.03 LKK360 pKa = 10.84 ACSHH364 pKa = 6.45 GKK366 pKa = 10.23 CILIRR371 pKa = 11.84 MSNRR375 pKa = 11.84 GMVKK379 pKa = 9.12 LTRR382 pKa = 11.84 GTTVEE387 pKa = 4.41 TIRR390 pKa = 11.84 CGTEE394 pKa = 3.96 CMLPPLDD401 pKa = 4.28 GEE403 pKa = 4.31 GDD405 pKa = 3.64 ILIDD409 pKa = 3.94 CPGGTQHH416 pKa = 7.24 FLQKK420 pKa = 10.9 NIIDD424 pKa = 4.67 LDD426 pKa = 4.15 CPNYY430 pKa = 10.19 PYY432 pKa = 10.61 FKK434 pKa = 10.91 EE435 pKa = 3.93 FMLYY439 pKa = 9.41 VCRR442 pKa = 11.84 ASHH445 pKa = 6.75 RR446 pKa = 11.84 PKK448 pKa = 8.43 TTIAFFAWLSVGYY461 pKa = 9.79 ILLSTVCSISLWSLKK476 pKa = 10.14 IMCKK480 pKa = 10.35 GIEE483 pKa = 4.09 LCKK486 pKa = 10.73 SKK488 pKa = 10.76 LPHH491 pKa = 6.65 NSGEE495 pKa = 4.35 CKK497 pKa = 9.97 VCRR500 pKa = 11.84 QHH502 pKa = 6.98 YY503 pKa = 10.0 SSEE506 pKa = 4.19 LGQQLHH512 pKa = 6.16 EE513 pKa = 4.65 ANCKK517 pKa = 10.4 NGLCPYY523 pKa = 9.85 CSNRR527 pKa = 11.84 LPEE530 pKa = 4.07 NSLYY534 pKa = 10.78 KK535 pKa = 10.27 HH536 pKa = 6.71 AEE538 pKa = 3.6 VCPRR542 pKa = 11.84 RR543 pKa = 11.84 KK544 pKa = 8.97 VTEE547 pKa = 3.82 DD548 pKa = 3.46 TIRR551 pKa = 11.84 EE552 pKa = 4.0 HH553 pKa = 7.89 DD554 pKa = 4.83 DD555 pKa = 3.67 FNSTPWFFVFIFGISEE571 pKa = 4.2 YY572 pKa = 10.68 KK573 pKa = 9.47 GTTIKK578 pKa = 10.69 RR579 pKa = 11.84 ATWLMILLALLLVSLSPVYY598 pKa = 10.87 GNEE601 pKa = 4.01 LFFDD605 pKa = 5.28 GIGDD609 pKa = 3.87 GQLEE613 pKa = 4.1 KK614 pKa = 11.05 GLWDD618 pKa = 3.62 EE619 pKa = 5.02 EE620 pKa = 4.03 IGLVEE625 pKa = 4.4 NCHH628 pKa = 5.22 QEE630 pKa = 4.14 CFITEE635 pKa = 4.28 TEE637 pKa = 4.24 CLCPSFEE644 pKa = 4.33 TGRR647 pKa = 11.84 KK648 pKa = 9.12 LLFFHH653 pKa = 7.4 LLNKK657 pKa = 9.92 QIRR660 pKa = 11.84 SGRR663 pKa = 11.84 KK664 pKa = 7.83 MKK666 pKa = 10.49 LLSSISLDD674 pKa = 3.92 TPWGVVKK681 pKa = 10.32 IEE683 pKa = 4.22 KK684 pKa = 10.05 SFKK687 pKa = 10.05 PVLSMSNLQLSWTSEE702 pKa = 3.98 EE703 pKa = 4.2 EE704 pKa = 4.05 VGGKK708 pKa = 9.46 IVVSGKK714 pKa = 8.56 STAVLKK720 pKa = 10.94 LKK722 pKa = 10.45 EE723 pKa = 4.02 KK724 pKa = 10.19 TGMVWEE730 pKa = 4.9 LSSSKK735 pKa = 9.85 ATEE738 pKa = 3.89 KK739 pKa = 9.78 KK740 pKa = 8.31 TLIVSVMDD748 pKa = 3.04 YY749 pKa = 8.34 TQEE752 pKa = 4.07 YY753 pKa = 7.67 KK754 pKa = 9.59 TQLQYY759 pKa = 10.78 LTGDD763 pKa = 3.65 RR764 pKa = 11.84 LVSEE768 pKa = 5.04 WPRR771 pKa = 11.84 ATCTGPCPDD780 pKa = 3.96 RR781 pKa = 11.84 CACHH785 pKa = 6.43 TSTCTWKK792 pKa = 10.39 AWPNSRR798 pKa = 11.84 KK799 pKa = 6.4 WTCNPTWCWGVGTGCTCCGMDD820 pKa = 4.97 IEE822 pKa = 5.67 KK823 pKa = 10.29 PYY825 pKa = 11.1 QNYY828 pKa = 9.41 LAAKK832 pKa = 9.26 WSTEE836 pKa = 4.0 YY837 pKa = 10.61 IKK839 pKa = 10.14 TDD841 pKa = 3.03 VVVCVEE847 pKa = 4.06 TSDD850 pKa = 4.32 EE851 pKa = 4.21 EE852 pKa = 4.28 RR853 pKa = 11.84 HH854 pKa = 6.14 CDD856 pKa = 3.29 IIQAGSRR863 pKa = 11.84 FHH865 pKa = 7.14 LGPITVLISDD875 pKa = 4.18 PQNVVKK881 pKa = 10.78 KK882 pKa = 10.35 LPSEE886 pKa = 3.97 VATIHH891 pKa = 6.32 KK892 pKa = 8.83 VQSSEE897 pKa = 3.89 VDD899 pKa = 3.32 VMHH902 pKa = 7.2 VDD904 pKa = 5.21 RR905 pKa = 11.84 ILTANGLCKK914 pKa = 10.32 LQSCTHH920 pKa = 6.82 GSPGDD925 pKa = 3.62 IQIFKK930 pKa = 10.16 PDD932 pKa = 3.8 YY933 pKa = 9.87 LVKK936 pKa = 10.71 HH937 pKa = 6.62 SISKK941 pKa = 10.26 RR942 pKa = 11.84 INAIEE947 pKa = 4.03 DD948 pKa = 3.34 HH949 pKa = 5.95 WWANDD954 pKa = 2.88 TWMSWQGTDD963 pKa = 3.71 LDD965 pKa = 4.85 YY966 pKa = 11.75 YY967 pKa = 9.43 CTTGSWPTCTYY978 pKa = 10.78 SGVVRR983 pKa = 11.84 QNTDD987 pKa = 2.28 SFKK990 pKa = 11.07 NLEE993 pKa = 4.13 TTEE996 pKa = 4.16 FDD998 pKa = 3.62 LLEE1001 pKa = 4.42 EE1002 pKa = 4.49 YY1003 pKa = 10.32 FFHH1006 pKa = 7.15 SSRR1009 pKa = 11.84 VEE1011 pKa = 3.66 VHH1013 pKa = 6.19 GRR1015 pKa = 11.84 TLNFPVKK1022 pKa = 10.22 SRR1024 pKa = 11.84 PKK1026 pKa = 9.85 EE1027 pKa = 3.87 GGGEE1031 pKa = 4.0 LTVLVEE1037 pKa = 4.22 VNGLEE1042 pKa = 4.12 LHH1044 pKa = 6.71 SKK1046 pKa = 10.63 LIVPVGLMFKK1056 pKa = 9.03 ITSCKK1061 pKa = 10.02 GCYY1064 pKa = 9.29 SCSSGFICDD1073 pKa = 3.26 VVLGVDD1079 pKa = 4.25 SPPEE1083 pKa = 3.97 LTVHH1087 pKa = 7.2 AEE1089 pKa = 4.06 CTNPNIVLTEE1099 pKa = 3.97 GSLIARR1105 pKa = 11.84 QGQTSTAKK1113 pKa = 10.07 IKK1115 pKa = 10.69 GFSVLKK1121 pKa = 6.87 TTRR1124 pKa = 11.84 LCIVLQEE1131 pKa = 4.38 SKK1133 pKa = 11.04 VLEE1136 pKa = 4.28 KK1137 pKa = 10.81 LIEE1140 pKa = 4.59 DD1141 pKa = 3.9 CTEE1144 pKa = 4.27 LKK1146 pKa = 10.74 LDD1148 pKa = 4.25 DD1149 pKa = 4.89 PKK1151 pKa = 11.55 DD1152 pKa = 3.86 VIIEE1156 pKa = 4.18 RR1157 pKa = 11.84 GSTLLSHH1164 pKa = 6.6 QNDD1167 pKa = 3.63 SCSYY1171 pKa = 11.55 GLGCWLANVGTFGSGLSMIFQNYY1194 pKa = 8.09 FGSVILGFLFFVLPVVLLLVFFCLGDD1220 pKa = 3.53 KK1221 pKa = 10.63 IFFCRR1226 pKa = 11.84 KK1227 pKa = 9.3 FKK1229 pKa = 9.97 WCFKK1233 pKa = 10.62 SNKK1236 pKa = 7.92 EE1237 pKa = 4.01 DD1238 pKa = 3.5 KK1239 pKa = 10.74 EE1240 pKa = 4.22 KK1241 pKa = 10.48 FKK1243 pKa = 11.47 QMVTEE1248 pKa = 4.64 LKK1250 pKa = 8.84 QTNLIKK1256 pKa = 10.69 KK1257 pKa = 8.22 MKK1259 pKa = 10.14 EE1260 pKa = 3.56 EE1261 pKa = 4.81 AKK1263 pKa = 10.12 TSWRR1267 pKa = 11.84 GLANKK1272 pKa = 10.16 ALGKK1276 pKa = 8.02 STKK1279 pKa = 10.38 EE1280 pKa = 3.79 EE1281 pKa = 3.96
Molecular weight: 143.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.931
IPC2_protein 7.19
IPC_protein 6.956
Toseland 6.664
ProMoST 7.585
Dawson 7.658
Bjellqvist 8.2
Wikipedia 7.556
Rodwell 7.688
Grimsley 6.707
Solomon 7.673
Lehninger 7.688
Nozaki 8.551
DTASelect 7.849
Thurlkill 7.907
EMBOSS 7.892
Sillero 8.273
Patrickios 3.948
IPC_peptide 7.673
IPC2_peptide 7.366
IPC2.peptide.svr19 7.135
Protein with the highest isoelectric point:
>tr|A0A0M5KMK1|A0A0M5KMK1_9VIRU Glycoprotein G2 OS=Thiafora orthonairovirus OX=1980529 PE=4 SV=1
MM1 pKa = 8.01 DD2 pKa = 5.13 ALEE5 pKa = 4.47 RR6 pKa = 11.84 LDD8 pKa = 4.15 SIVWEE13 pKa = 3.96 ILGEE17 pKa = 4.26 SFSRR21 pKa = 11.84 AFLSLDD27 pKa = 3.12 LHH29 pKa = 6.78 AFFRR33 pKa = 11.84 VNRR36 pKa = 11.84 EE37 pKa = 3.98 VGDD40 pKa = 3.87 GNCFYY45 pKa = 10.88 RR46 pKa = 11.84 ALSRR50 pKa = 11.84 LHH52 pKa = 6.94 SKK54 pKa = 10.9 DD55 pKa = 3.16 KK56 pKa = 11.09 TSDD59 pKa = 2.68 EE60 pKa = 4.0 HH61 pKa = 8.47 LYY63 pKa = 10.71 YY64 pKa = 10.81 RR65 pKa = 11.84 LLIPDD70 pKa = 4.29 AAQRR74 pKa = 11.84 YY75 pKa = 8.57 FDD77 pKa = 4.09 TEE79 pKa = 3.99 QEE81 pKa = 4.2 AVGLGLTKK89 pKa = 10.59 EE90 pKa = 4.65 EE91 pKa = 4.29 YY92 pKa = 10.33 ASRR95 pKa = 11.84 AILDD99 pKa = 4.13 GEE101 pKa = 4.31 WAGSLEE107 pKa = 3.94 ASMLSKK113 pKa = 10.58 FLDD116 pKa = 3.06 ITIIIWIIDD125 pKa = 3.55 GSGTITSAQRR135 pKa = 11.84 YY136 pKa = 8.17 GNNKK140 pKa = 8.69 PSKK143 pKa = 10.35 AYY145 pKa = 9.92 NLCLIGNTHH154 pKa = 6.84 FDD156 pKa = 3.22 SLTIQTSNIQHH167 pKa = 6.92 RR168 pKa = 11.84 SQLSLMGRR176 pKa = 11.84 LEE178 pKa = 4.46 SIEE181 pKa = 4.39 EE182 pKa = 4.07 LTSLDD187 pKa = 3.96 EE188 pKa = 4.33 EE189 pKa = 5.08 SLFSTHH195 pKa = 6.74 HH196 pKa = 7.05 RR197 pKa = 11.84 DD198 pKa = 2.86 LSTTVEE204 pKa = 4.17 VKK206 pKa = 9.08 TLKK209 pKa = 10.03 TKK211 pKa = 10.4 LFPQKK216 pKa = 9.35 TVLKK220 pKa = 9.47 MIEE223 pKa = 4.11 KK224 pKa = 10.19 SQGIPLRR231 pKa = 11.84 IGRR234 pKa = 11.84 IVEE237 pKa = 4.07 LLFSCRR243 pKa = 11.84 LGFSISPGLLKK254 pKa = 10.26 IAILTEE260 pKa = 4.09 EE261 pKa = 4.29 TDD263 pKa = 3.66 SIFDD267 pKa = 3.45 IRR269 pKa = 11.84 KK270 pKa = 9.5 LGHH273 pKa = 6.74 HH274 pKa = 7.22 LLTNEE279 pKa = 3.71 KK280 pKa = 9.94 RR281 pKa = 11.84 IKK283 pKa = 10.62 KK284 pKa = 9.2 EE285 pKa = 3.76 FSNCHH290 pKa = 6.0 LKK292 pKa = 10.01 IDD294 pKa = 4.33 DD295 pKa = 4.81 DD296 pKa = 4.23 VWPHH300 pKa = 6.77 LDD302 pKa = 3.08 EE303 pKa = 6.0 SYY305 pKa = 11.41 LLRR308 pKa = 11.84 FAFPGYY314 pKa = 9.57 GLHH317 pKa = 6.86 RR318 pKa = 11.84 FIPMLLPSIIEE329 pKa = 4.21 DD330 pKa = 3.74 TLKK333 pKa = 10.75 VCLAILLSSFLYY345 pKa = 10.0 KK346 pKa = 10.62 SKK348 pKa = 10.49 VRR350 pKa = 11.84 YY351 pKa = 9.31 KK352 pKa = 10.75 KK353 pKa = 10.48 EE354 pKa = 4.18 FIVNCCKK361 pKa = 10.07 STIVSGKK368 pKa = 9.87 RR369 pKa = 11.84 VGRR372 pKa = 11.84 SIKK375 pKa = 9.82 RR376 pKa = 11.84 TTTADD381 pKa = 3.6 LYY383 pKa = 10.88 NSPRR387 pKa = 11.84 TILKK391 pKa = 8.9 NCCEE395 pKa = 4.07 AVFGKK400 pKa = 10.59 LVVKK404 pKa = 10.27 IMKK407 pKa = 9.46 YY408 pKa = 8.64 IKK410 pKa = 10.65 AMNGVNHH417 pKa = 7.17 LLLRR421 pKa = 11.84 NLDD424 pKa = 4.41 FSALSLPDD432 pKa = 3.55 YY433 pKa = 10.54 LRR435 pKa = 11.84 LMKK438 pKa = 10.04 TLAEE442 pKa = 4.43 EE443 pKa = 4.09 DD444 pKa = 4.42 LKK446 pKa = 11.45 DD447 pKa = 3.31 HH448 pKa = 6.78 NYY450 pKa = 9.68 ISKK453 pKa = 10.34 EE454 pKa = 3.76 LASLNKK460 pKa = 10.17 LNADD464 pKa = 3.69 LKK466 pKa = 10.51 VLKK469 pKa = 10.36 EE470 pKa = 4.25 DD471 pKa = 4.22 GDD473 pKa = 3.75 WEE475 pKa = 4.44 GRR477 pKa = 11.84 AKK479 pKa = 10.52 EE480 pKa = 3.93 DD481 pKa = 3.69 LIARR485 pKa = 11.84 FFEE488 pKa = 4.49 EE489 pKa = 4.26 KK490 pKa = 10.7 NLLKK494 pKa = 10.64 FVGKK498 pKa = 8.47 TGKK501 pKa = 10.44 ASGSFQIGNVLAYY514 pKa = 10.25 AYY516 pKa = 10.79 NLYY519 pKa = 10.66 LNKK522 pKa = 9.69 DD523 pKa = 3.76 TLNLSDD529 pKa = 4.72 EE530 pKa = 4.43 DD531 pKa = 4.05 VEE533 pKa = 4.43 QISIEE538 pKa = 3.94 IRR540 pKa = 11.84 KK541 pKa = 8.79 LQLLQEE547 pKa = 4.75 GEE549 pKa = 4.32 TFEE552 pKa = 4.48 PVAIICSKK560 pKa = 11.19 LEE562 pKa = 3.8 AFFEE566 pKa = 4.69 KK567 pKa = 10.65 AFSRR571 pKa = 11.84 LPEE574 pKa = 4.17 HH575 pKa = 6.88 CKK577 pKa = 10.39 SEE579 pKa = 4.15 CRR581 pKa = 11.84 VLFNDD586 pKa = 2.85 VRR588 pKa = 11.84 NAVSHH593 pKa = 5.3 SVAWKK598 pKa = 9.28 HH599 pKa = 5.66 ALRR602 pKa = 11.84 LKK604 pKa = 8.93 GTLYY608 pKa = 10.85 EE609 pKa = 4.7 GFFSLQYY616 pKa = 8.4 GWTYY620 pKa = 10.49 IPEE623 pKa = 4.41 DD624 pKa = 3.91 LKK626 pKa = 10.21 PTLMMTIQTLFPEE639 pKa = 4.57 KK640 pKa = 10.05 FVQFLEE646 pKa = 4.29 KK647 pKa = 9.78 TQLHH651 pKa = 6.66 PEE653 pKa = 4.11 FRR655 pKa = 11.84 DD656 pKa = 3.47 LTPDD660 pKa = 3.41 YY661 pKa = 11.39 IMTQRR666 pKa = 11.84 LMMEE670 pKa = 4.55 GDD672 pKa = 3.53 NPKK675 pKa = 10.85 LNTNSQLKK683 pKa = 9.85 ILEE686 pKa = 4.32 GLQEE690 pKa = 4.37 SVEE693 pKa = 4.32 TIPVGKK699 pKa = 10.09 NIFPLPEE706 pKa = 3.77 VAVDD710 pKa = 4.48 EE711 pKa = 4.41 IRR713 pKa = 11.84 SVDD716 pKa = 3.71 SVIEE720 pKa = 4.22 KK721 pKa = 10.05 IRR723 pKa = 11.84 NLAQRR728 pKa = 11.84 SNSRR732 pKa = 11.84 QNRR735 pKa = 11.84 FHH737 pKa = 8.56 IEE739 pKa = 3.89 EE740 pKa = 4.24 NNVANSQEE748 pKa = 4.07 RR749 pKa = 11.84 SFSVHH754 pKa = 5.38 QLLLVEE760 pKa = 4.83 VGYY763 pKa = 8.11 QTDD766 pKa = 3.59 VEE768 pKa = 4.83 GKK770 pKa = 10.08 VLTDD774 pKa = 3.21 TVKK777 pKa = 9.93 WKK779 pKa = 10.6 EE780 pKa = 4.0 VLKK783 pKa = 10.89 LLAFMGIKK791 pKa = 9.02 ATLIVCVDD799 pKa = 3.31 NTKK802 pKa = 8.34 THH804 pKa = 6.29 VNDD807 pKa = 2.65 WWIDD811 pKa = 3.21 EE812 pKa = 4.56 DD813 pKa = 4.31 YY814 pKa = 11.68 VRR816 pKa = 11.84 LIKK819 pKa = 10.79 NSISHH824 pKa = 7.11 LFSKK828 pKa = 10.49 LSKK831 pKa = 8.35 NTPVEE836 pKa = 4.1 VTDD839 pKa = 3.69 VVVGSISTQKK849 pKa = 9.75 IRR851 pKa = 11.84 SFLKK855 pKa = 10.35 SGSSTKK861 pKa = 10.27 TPLSTKK867 pKa = 10.2 DD868 pKa = 3.42 VQEE871 pKa = 3.78 TWKK874 pKa = 10.91 AMKK877 pKa = 10.55 NFILEE882 pKa = 4.03 RR883 pKa = 11.84 DD884 pKa = 3.4 TGVILGNEE892 pKa = 4.44 YY893 pKa = 10.56 SSPMYY898 pKa = 9.37 MGLVEE903 pKa = 4.72 GVTISEE909 pKa = 3.95 EE910 pKa = 4.1 GARR913 pKa = 11.84 TVIDD917 pKa = 3.86 LLKK920 pKa = 10.53 EE921 pKa = 4.02 NMVSITDD928 pKa = 3.38 EE929 pKa = 4.35 FEE931 pKa = 3.86 RR932 pKa = 11.84 TRR934 pKa = 11.84 YY935 pKa = 8.88 AHH937 pKa = 6.42 EE938 pKa = 4.22 VNKK941 pKa = 10.61 KK942 pKa = 10.11 IITSEE947 pKa = 4.0 KK948 pKa = 10.75 LLLAWLKK955 pKa = 10.88 EE956 pKa = 4.35 DD957 pKa = 3.95 IKK959 pKa = 10.81 GTRR962 pKa = 11.84 CEE964 pKa = 4.23 DD965 pKa = 3.67 CVHH968 pKa = 6.89 EE969 pKa = 5.62 IITSSNGAITNKK981 pKa = 10.44 SKK983 pKa = 11.36 LNLLARR989 pKa = 11.84 ACTFASHH996 pKa = 7.3 PACCHH1001 pKa = 6.58 PITVKK1006 pKa = 10.35 INNRR1010 pKa = 11.84 SNFYY1014 pKa = 10.82 KK1015 pKa = 10.6 RR1016 pKa = 11.84 IPDD1019 pKa = 3.21 IDD1021 pKa = 5.2 LISHH1025 pKa = 7.15 LNLKK1029 pKa = 10.34 SLDD1032 pKa = 3.93 DD1033 pKa = 4.09 EE1034 pKa = 4.53 EE1035 pKa = 6.26 DD1036 pKa = 4.1 RR1037 pKa = 11.84 ITDD1040 pKa = 3.76 LDD1042 pKa = 3.72 RR1043 pKa = 11.84 LVRR1046 pKa = 11.84 LTIPGKK1052 pKa = 7.33 TEE1054 pKa = 3.57 KK1055 pKa = 10.15 EE1056 pKa = 4.06 KK1057 pKa = 10.77 KK1058 pKa = 9.18 IKK1060 pKa = 10.47 RR1061 pKa = 11.84 SVDD1064 pKa = 3.38 CLIKK1068 pKa = 11.04 LMMLKK1073 pKa = 10.45 SSIEE1077 pKa = 4.25 CIKK1080 pKa = 10.82 LPTGQIVVLDD1090 pKa = 4.1 QITKK1094 pKa = 10.32 NNITRR1099 pKa = 11.84 STEE1102 pKa = 3.38 SDD1104 pKa = 2.92 RR1105 pKa = 11.84 RR1106 pKa = 11.84 KK1107 pKa = 6.78 TTNNRR1112 pKa = 11.84 EE1113 pKa = 3.82 DD1114 pKa = 4.0 TIVKK1118 pKa = 10.0 NLSQVKK1124 pKa = 9.31 LAGYY1128 pKa = 9.72 SDD1130 pKa = 4.12 YY1131 pKa = 11.07 VKK1133 pKa = 10.58 QVIGSSIKK1141 pKa = 11.02 NMDD1144 pKa = 3.26 KK1145 pKa = 10.51 QQTSSCRR1152 pKa = 11.84 LDD1154 pKa = 3.61 KK1155 pKa = 11.12 AWLEE1159 pKa = 4.1 KK1160 pKa = 9.95 LTADD1164 pKa = 5.09 LNVPMHH1170 pKa = 6.52 NEE1172 pKa = 3.93 DD1173 pKa = 4.01 IIAKK1177 pKa = 8.48 VKK1179 pKa = 9.68 RR1180 pKa = 11.84 TIEE1183 pKa = 3.7 ARR1185 pKa = 11.84 KK1186 pKa = 9.79 EE1187 pKa = 3.75 YY1188 pKa = 10.23 IRR1190 pKa = 11.84 NNDD1193 pKa = 3.28 KK1194 pKa = 11.07 LLVRR1198 pKa = 11.84 SPHH1201 pKa = 6.49 EE1202 pKa = 3.38 IAGYY1206 pKa = 10.82 LNGLKK1211 pKa = 10.63 GNLCGEE1217 pKa = 4.84 PKK1219 pKa = 10.39 RR1220 pKa = 11.84 KK1221 pKa = 9.54 IFTVDD1226 pKa = 2.94 CVLFKK1231 pKa = 10.95 EE1232 pKa = 4.93 VVAEE1236 pKa = 4.17 AMLRR1240 pKa = 11.84 YY1241 pKa = 9.77 QSTAYY1246 pKa = 9.7 QGCVDD1251 pKa = 4.13 HH1252 pKa = 6.84 MVMLLEE1258 pKa = 4.77 LLLEE1262 pKa = 4.29 FTWFQEE1268 pKa = 3.69 VLLYY1272 pKa = 11.05 SKK1274 pKa = 10.25 ICEE1277 pKa = 4.12 TFLRR1281 pKa = 11.84 ICTEE1285 pKa = 4.01 FNRR1288 pKa = 11.84 AGLKK1292 pKa = 10.03 LLRR1295 pKa = 11.84 IRR1297 pKa = 11.84 HH1298 pKa = 5.44 LNINIGVKK1306 pKa = 10.5 LPANKK1311 pKa = 9.09 KK1312 pKa = 9.85 QNMQCRR1318 pKa = 11.84 IYY1320 pKa = 11.09 DD1321 pKa = 3.98 SVSKK1325 pKa = 10.45 PLTEE1329 pKa = 3.95 VFFLNRR1335 pKa = 11.84 RR1336 pKa = 11.84 QAIIGAAYY1344 pKa = 8.88 PYY1346 pKa = 10.12 ILLVLYY1352 pKa = 7.78 MQVLQQQRR1360 pKa = 11.84 CIEE1363 pKa = 4.02 EE1364 pKa = 3.86 LGNRR1368 pKa = 11.84 GSHH1371 pKa = 4.97 VQGIRR1376 pKa = 11.84 NKK1378 pKa = 10.13 SSKK1381 pKa = 10.75 LLDD1384 pKa = 3.38 VFANEE1389 pKa = 5.53 AISILNGQFEE1399 pKa = 4.67 DD1400 pKa = 3.6 AFKK1403 pKa = 11.05 EE1404 pKa = 3.96 RR1405 pKa = 11.84 LHH1407 pKa = 6.36 LCRR1410 pKa = 11.84 SAGNFFSKK1418 pKa = 10.34 PSYY1421 pKa = 10.59 EE1422 pKa = 3.83 NFINVISGLNLVYY1435 pKa = 10.4 GVIMKK1440 pKa = 10.58 DD1441 pKa = 2.95 SFLANSQPQNKK1452 pKa = 7.85 QLQMLRR1458 pKa = 11.84 YY1459 pKa = 9.81 GMLNGLSRR1467 pKa = 11.84 LSCPSEE1473 pKa = 3.9 LGKK1476 pKa = 10.72 KK1477 pKa = 10.35 FSSSCRR1483 pKa = 11.84 RR1484 pKa = 11.84 MEE1486 pKa = 4.87 DD1487 pKa = 3.13 NLSRR1491 pKa = 11.84 IYY1493 pKa = 10.65 LQAAVYY1499 pKa = 9.73 YY1500 pKa = 9.13 SSRR1503 pKa = 11.84 DD1504 pKa = 3.18 IEE1506 pKa = 4.58 CNIKK1510 pKa = 9.78 AWKK1513 pKa = 8.43 EE1514 pKa = 3.56 TDD1516 pKa = 3.43 LCPQTTIPCFTVYY1529 pKa = 11.02 GLFVNSDD1536 pKa = 3.31 RR1537 pKa = 11.84 QLVFDD1542 pKa = 4.83 IYY1544 pKa = 11.3 NVHH1547 pKa = 6.96 IYY1549 pKa = 9.8 NKK1551 pKa = 10.3 EE1552 pKa = 3.56 MDD1554 pKa = 5.31 DD1555 pKa = 3.85 FDD1557 pKa = 4.31 EE1558 pKa = 5.13 GCISVLEE1565 pKa = 4.36 EE1566 pKa = 3.38 TAEE1569 pKa = 3.74 RR1570 pKa = 11.84 HH1571 pKa = 5.55 MNWEE1575 pKa = 3.96 MDD1577 pKa = 3.75 LEE1579 pKa = 4.35 EE1580 pKa = 4.07 SWRR1583 pKa = 11.84 VKK1585 pKa = 9.48 DD1586 pKa = 3.95 QRR1588 pKa = 11.84 SCRR1591 pKa = 11.84 LLLGIPNIHH1600 pKa = 6.45 RR1601 pKa = 11.84 SSRR1604 pKa = 11.84 KK1605 pKa = 9.52 KK1606 pKa = 9.12 EE1607 pKa = 3.87 AKK1609 pKa = 10.34 FSDD1612 pKa = 4.33 DD1613 pKa = 4.02 GSDD1616 pKa = 3.15 TNTPKK1621 pKa = 10.82 DD1622 pKa = 3.11 SCLTRR1627 pKa = 11.84 RR1628 pKa = 11.84 SSSSKK1633 pKa = 9.97 RR1634 pKa = 11.84 SSASSLINRR1643 pKa = 11.84 YY1644 pKa = 5.41 TTIIKK1649 pKa = 9.88 PIEE1652 pKa = 4.11 IDD1654 pKa = 3.02 SGIFIEE1660 pKa = 5.31 SDD1662 pKa = 3.25 SLRR1665 pKa = 11.84 QGRR1668 pKa = 11.84 AAATGGPKK1676 pKa = 10.14 YY1677 pKa = 11.01 YY1678 pKa = 10.66 SYY1680 pKa = 10.7 TPNKK1684 pKa = 10.36 ASVLKK1689 pKa = 10.49 DD1690 pKa = 3.16 CMSIIRR1696 pKa = 11.84 KK1697 pKa = 8.52 NPNYY1701 pKa = 10.43 SYY1703 pKa = 11.75 GSFEE1707 pKa = 5.41 LIQAITEE1714 pKa = 4.44 FARR1717 pKa = 11.84 KK1718 pKa = 9.29 KK1719 pKa = 10.19 YY1720 pKa = 9.48 PEE1722 pKa = 4.04 EE1723 pKa = 5.47 SIMRR1727 pKa = 11.84 AKK1729 pKa = 10.28 RR1730 pKa = 11.84 DD1731 pKa = 3.49 PKK1733 pKa = 9.77 NWVSVSEE1740 pKa = 4.31 VTEE1743 pKa = 4.14 TTSIVSTPRR1752 pKa = 11.84 TQFYY1756 pKa = 11.25 VKK1758 pKa = 10.52 DD1759 pKa = 3.73 CFKK1762 pKa = 11.08 INLSNQNKK1770 pKa = 9.81 KK1771 pKa = 9.72 ISKK1774 pKa = 10.14 LIRR1777 pKa = 11.84 NKK1779 pKa = 10.23 LKK1781 pKa = 10.82 KK1782 pKa = 10.27 LGSLFSDD1789 pKa = 3.13 NDD1791 pKa = 3.38 VSRR1794 pKa = 11.84 KK1795 pKa = 9.7 DD1796 pKa = 3.61 CSILLSSVDD1805 pKa = 4.51 GLTEE1809 pKa = 3.88 QQKK1812 pKa = 11.02 KK1813 pKa = 10.57 DD1814 pKa = 3.2 VTNAVFEE1821 pKa = 4.17 PSKK1824 pKa = 10.73 LSIYY1828 pKa = 9.61 NWSHH1832 pKa = 6.66 ILTKK1836 pKa = 10.39 SIYY1839 pKa = 9.67 EE1840 pKa = 3.97 VLLTHH1845 pKa = 7.42 DD1846 pKa = 4.63 GNIIYY1851 pKa = 9.94 CWIKK1855 pKa = 10.74 SLSLMVKK1862 pKa = 9.8 SRR1864 pKa = 11.84 LRR1866 pKa = 11.84 KK1867 pKa = 8.42 HH1868 pKa = 6.01 LSFMGVEE1875 pKa = 4.31 RR1876 pKa = 11.84 EE1877 pKa = 4.49 DD1878 pKa = 3.73 IPDD1881 pKa = 3.36 EE1882 pKa = 4.3 GFFAKK1887 pKa = 10.67 DD1888 pKa = 3.65 EE1889 pKa = 4.25 IDD1891 pKa = 3.47 SLIEE1895 pKa = 3.79 VRR1897 pKa = 11.84 KK1898 pKa = 9.98 LLVCKK1903 pKa = 10.36 EE1904 pKa = 3.92 SDD1906 pKa = 3.87 EE1907 pKa = 4.24 ISSVHH1912 pKa = 5.75 IPGIIKK1918 pKa = 10.25 AWIKK1922 pKa = 10.7 CIFIKK1927 pKa = 10.49 PIEE1930 pKa = 4.4 NIYY1933 pKa = 10.81 NYY1935 pKa = 11.1 SLLQKK1940 pKa = 9.59 MLVAAEE1946 pKa = 4.03 DD1947 pKa = 4.37 LYY1949 pKa = 11.3 AIRR1952 pKa = 11.84 LEE1954 pKa = 4.18 HH1955 pKa = 6.6 MLFLRR1960 pKa = 11.84 DD1961 pKa = 3.98 KK1962 pKa = 10.4 RR1963 pKa = 11.84 DD1964 pKa = 3.14 NSYY1967 pKa = 11.51 SSFIKK1972 pKa = 10.73 EE1973 pKa = 3.88 EE1974 pKa = 3.93 LTLKK1978 pKa = 10.63 GKK1980 pKa = 9.92 EE1981 pKa = 3.53 RR1982 pKa = 11.84 DD1983 pKa = 3.56 FLKK1986 pKa = 10.42 KK1987 pKa = 10.33 YY1988 pKa = 10.71 SEE1990 pKa = 4.7 TIVKK1994 pKa = 9.84 SVNFLFFVAVSAPWCMHH2011 pKa = 4.99 YY2012 pKa = 10.43 KK2013 pKa = 10.42 ALEE2016 pKa = 4.44 AYY2018 pKa = 9.44 LVKK2021 pKa = 10.52 HH2022 pKa = 5.92 PEE2024 pKa = 3.79 ILDD2027 pKa = 3.72 VNDD2030 pKa = 4.78 SEE2032 pKa = 4.73 TSTNGILSMTVGNIFFEE2049 pKa = 4.83 LYY2051 pKa = 10.53 KK2052 pKa = 10.55 DD2053 pKa = 3.86 FCVKK2057 pKa = 10.55 EE2058 pKa = 4.21 SVNVDD2063 pKa = 2.9 KK2064 pKa = 11.21 RR2065 pKa = 11.84 KK2066 pKa = 9.54 MITLRR2071 pKa = 11.84 FYY2073 pKa = 11.25 VRR2075 pKa = 11.84 YY2076 pKa = 7.79 LTTMFSSNTEE2086 pKa = 3.86 PFSASLNDD2094 pKa = 4.7 DD2095 pKa = 4.58 EE2096 pKa = 6.79 IDD2098 pKa = 3.59 MTGSTDD2104 pKa = 3.55 VEE2106 pKa = 4.38 EE2107 pKa = 4.83 KK2108 pKa = 10.87 LLSQTKK2114 pKa = 9.76 RR2115 pKa = 11.84 VFAKK2119 pKa = 9.87 MGLSDD2124 pKa = 4.28 KK2125 pKa = 10.97 SYY2127 pKa = 11.04 DD2128 pKa = 4.32 FIWTVQMIANSNFNVCKK2145 pKa = 10.16 KK2146 pKa = 10.57 LSGRR2150 pKa = 11.84 SEE2152 pKa = 4.17 GEE2154 pKa = 3.56 RR2155 pKa = 11.84 LPRR2158 pKa = 11.84 SIRR2161 pKa = 11.84 SKK2163 pKa = 10.67 VVYY2166 pKa = 10.47 EE2167 pKa = 3.82 MVKK2170 pKa = 10.54 LVGEE2174 pKa = 4.18 SGMAILQQLAFAKK2187 pKa = 10.25 SLNYY2191 pKa = 9.35 NHH2193 pKa = 7.33 RR2194 pKa = 11.84 FFSVLAPKK2202 pKa = 10.15 AQLGGSRR2209 pKa = 11.84 DD2210 pKa = 3.76 LLVQEE2215 pKa = 4.6 TGTKK2219 pKa = 9.41 IIHH2222 pKa = 6.66 AATEE2226 pKa = 4.38 SFSRR2230 pKa = 11.84 TLLRR2234 pKa = 11.84 TTNDD2238 pKa = 3.43 DD2239 pKa = 3.62 GLTNQYY2245 pKa = 10.97 LKK2247 pKa = 10.07 EE2248 pKa = 4.22 TVLNYY2253 pKa = 10.99 ALDD2256 pKa = 3.99 ALATMKK2262 pKa = 10.94 SLDD2265 pKa = 3.6 GEE2267 pKa = 4.61 SIKK2270 pKa = 11.16 NSTCLVNFYY2279 pKa = 10.72 RR2280 pKa = 11.84 VVCISGDD2287 pKa = 3.45 NTKK2290 pKa = 9.68 WGPIHH2295 pKa = 6.69 CCSFFSGMMQQLLRR2309 pKa = 11.84 EE2310 pKa = 4.4 HH2311 pKa = 7.39 PDD2313 pKa = 2.34 WSAFYY2318 pKa = 10.88 RR2319 pKa = 11.84 LTFIKK2324 pKa = 10.31 NLCRR2328 pKa = 11.84 QVEE2331 pKa = 4.31 IPAASIKK2338 pKa = 10.6 KK2339 pKa = 9.41 IINVAKK2345 pKa = 10.8 LEE2347 pKa = 4.52 LDD2349 pKa = 3.44 PKK2351 pKa = 10.86 QDD2353 pKa = 3.44 INSLSEE2359 pKa = 3.88 KK2360 pKa = 9.95 QAQEE2364 pKa = 4.11 LLSDD2368 pKa = 4.77 SAEE2371 pKa = 3.91 KK2372 pKa = 10.23 WNALPYY2378 pKa = 10.76 VKK2380 pKa = 10.24 FLILTYY2386 pKa = 10.46 LKK2388 pKa = 10.48 HH2389 pKa = 6.62 GKK2391 pKa = 9.55 LAMNSYY2397 pKa = 9.89 NHH2399 pKa = 6.35 MGQGIHH2405 pKa = 6.63 HH2406 pKa = 6.82 ATSSILTSLMAEE2418 pKa = 4.22 VFEE2421 pKa = 5.02 DD2422 pKa = 3.87 LCVHH2426 pKa = 5.69 YY2427 pKa = 10.26 FKK2429 pKa = 10.46 TAFPNLTVNVNHH2441 pKa = 7.07 AGSSDD2446 pKa = 4.27 DD2447 pKa = 3.63 YY2448 pKa = 10.8 AKK2450 pKa = 10.76 CIILTGTVDD2459 pKa = 3.36 RR2460 pKa = 11.84 EE2461 pKa = 4.0 QYY2463 pKa = 10.14 EE2464 pKa = 4.46 LYY2466 pKa = 10.8 DD2467 pKa = 3.52 AVFWDD2472 pKa = 4.14 HH2473 pKa = 5.75 VCRR2476 pKa = 11.84 FKK2478 pKa = 11.43 NYY2480 pKa = 7.47 TAAVNRR2486 pKa = 11.84 CCQMKK2491 pKa = 10.58 DD2492 pKa = 3.05 SAKK2495 pKa = 9.95 TLVGDD2500 pKa = 4.69 CFLEE2504 pKa = 5.16 FYY2506 pKa = 11.04 SEE2508 pKa = 4.11 FMMGNRR2514 pKa = 11.84 ITPAVIKK2521 pKa = 9.71 FIFTGLINSSVTSPSSLTQACHH2543 pKa = 5.96 VSSQQAMYY2551 pKa = 10.85 NSVPMLTNIAFTLCRR2566 pKa = 11.84 QQMFFNHH2573 pKa = 5.19 VEE2575 pKa = 3.64 AFIRR2579 pKa = 11.84 KK2580 pKa = 8.95 FGPITLGSVSQFGRR2594 pKa = 11.84 LYY2596 pKa = 10.25 CPKK2599 pKa = 10.31 YY2600 pKa = 10.34 SNLVNTSVTIEE2611 pKa = 4.04 DD2612 pKa = 4.1 CEE2614 pKa = 5.21 SIVSACEE2621 pKa = 4.8 AINKK2625 pKa = 8.95 WDD2627 pKa = 3.77 MLFDD2631 pKa = 3.85 TAAKK2635 pKa = 10.22 SRR2637 pKa = 11.84 VEE2639 pKa = 3.89 EE2640 pKa = 3.98 EE2641 pKa = 3.9 LQKK2644 pKa = 10.91 KK2645 pKa = 9.8 HH2646 pKa = 6.11 EE2647 pKa = 4.35 EE2648 pKa = 4.08 EE2649 pKa = 4.26 TTLSSEE2655 pKa = 4.17 TSSLKK2660 pKa = 10.8 SGEE2663 pKa = 4.24 QKK2665 pKa = 10.63 SEE2667 pKa = 3.59 LTFIRR2672 pKa = 11.84 RR2673 pKa = 11.84 RR2674 pKa = 11.84 ALTEE2678 pKa = 3.85 DD2679 pKa = 3.32 EE2680 pKa = 4.37 VRR2682 pKa = 11.84 FLKK2685 pKa = 10.84 VSSEE2689 pKa = 3.56 EE2690 pKa = 3.73 RR2691 pKa = 11.84 RR2692 pKa = 11.84 YY2693 pKa = 8.83 TDD2695 pKa = 2.99 AQAVEE2700 pKa = 5.02 DD2701 pKa = 4.03 KK2702 pKa = 11.22 LKK2704 pKa = 10.87 VYY2706 pKa = 10.67 YY2707 pKa = 10.1 RR2708 pKa = 11.84 DD2709 pKa = 3.97 SQDD2712 pKa = 3.45 QNPKK2716 pKa = 7.99 NTEE2719 pKa = 4.23 FILDD2723 pKa = 3.72 SVLCNSCTWIQKK2735 pKa = 9.56 GKK2737 pKa = 10.59 EE2738 pKa = 3.76 KK2739 pKa = 10.93 GFLEE2743 pKa = 4.33 TAGRR2747 pKa = 11.84 IQMLLRR2753 pKa = 11.84 ILCFGHH2759 pKa = 6.04 YY2760 pKa = 9.86 RR2761 pKa = 11.84 SFCGQGIEE2769 pKa = 4.21 RR2770 pKa = 11.84 QVKK2773 pKa = 8.48 TSLNRR2778 pKa = 11.84 DD2779 pKa = 3.41 EE2780 pKa = 4.67 NQVIEE2785 pKa = 4.91 DD2786 pKa = 4.29 PMIQLIPEE2794 pKa = 3.98 KK2795 pKa = 10.35 LRR2797 pKa = 11.84 RR2798 pKa = 11.84 EE2799 pKa = 4.21 LEE2801 pKa = 4.02 RR2802 pKa = 11.84 LGLSRR2807 pKa = 11.84 MSIEE2811 pKa = 3.92 EE2812 pKa = 4.06 LLPRR2816 pKa = 11.84 GLNCATICQAVAHH2829 pKa = 6.46 KK2830 pKa = 10.39 LISLNVSTEE2839 pKa = 3.95 SYY2841 pKa = 8.04 TAEE2844 pKa = 3.92 VSRR2847 pKa = 11.84 LKK2849 pKa = 9.53 QTLTARR2855 pKa = 11.84 NVLYY2859 pKa = 10.87 GLAGGIKK2866 pKa = 9.42 EE2867 pKa = 4.23 LSIPIYY2873 pKa = 9.64 TIFMKK2878 pKa = 10.45 SYY2880 pKa = 9.27 FFKK2883 pKa = 11.26 DD2884 pKa = 3.34 NVFLDD2889 pKa = 3.78 LTDD2892 pKa = 3.2 RR2893 pKa = 11.84 WLTKK2897 pKa = 10.18 HH2898 pKa = 5.73 SSNYY2902 pKa = 9.5 RR2903 pKa = 11.84 DD2904 pKa = 3.53 STGKK2908 pKa = 10.71 KK2909 pKa = 9.58 LDD2911 pKa = 3.62 GKK2913 pKa = 10.48 IVTKK2917 pKa = 10.41 YY2918 pKa = 9.07 PHH2920 pKa = 6.54 WLSVFMNCLLAMDD2933 pKa = 4.6 RR2934 pKa = 11.84 TTVLSDD2940 pKa = 2.99 KK2941 pKa = 11.33 SLFNDD2946 pKa = 3.39 SLKK2949 pKa = 10.85 CISVTRR2955 pKa = 11.84 NLNEE2959 pKa = 3.86 QRR2961 pKa = 11.84 SLTLIQSHH2969 pKa = 6.3 LAVVSCEE2976 pKa = 3.87 LKK2978 pKa = 10.88 HH2979 pKa = 7.32 FILQFSNLNRR2989 pKa = 11.84 RR2990 pKa = 11.84 KK2991 pKa = 8.91 MKK2993 pKa = 10.02 IVEE2996 pKa = 4.24 SRR2998 pKa = 11.84 PPEE3001 pKa = 4.04 YY3002 pKa = 10.7 EE3003 pKa = 3.57 MEE3005 pKa = 4.29 ANKK3008 pKa = 9.94 VVITKK3013 pKa = 10.5 SSLFTAGDD3021 pKa = 3.95 GVKK3024 pKa = 10.61 LNNNPAVVVGFLLDD3038 pKa = 3.47 EE3039 pKa = 4.47 SSISEE3044 pKa = 4.11 VKK3046 pKa = 7.98 PTKK3049 pKa = 10.45 VDD3051 pKa = 3.91 FANLMKK3057 pKa = 10.76 DD3058 pKa = 2.89 KK3059 pKa = 10.92 FKK3061 pKa = 10.64 ISQYY3065 pKa = 9.72 FPSIEE3070 pKa = 4.04 AVLDD3074 pKa = 3.85 SLKK3077 pKa = 10.82 KK3078 pKa = 10.78 EE3079 pKa = 4.24 SDD3081 pKa = 3.14 LHH3083 pKa = 8.07 LSISSTPDD3091 pKa = 2.9 LQIATKK3097 pKa = 9.51 YY3098 pKa = 10.64 VNYY3101 pKa = 8.94 LTLLCRR3107 pKa = 11.84 MIIQTNSTLTVFYY3120 pKa = 9.6 MIKK3123 pKa = 10.48 GNKK3126 pKa = 7.72 LTNEE3130 pKa = 4.14 PTVSDD3135 pKa = 4.82 LICYY3139 pKa = 8.32 GIKK3142 pKa = 10.07 EE3143 pKa = 4.18 GRR3145 pKa = 11.84 HH3146 pKa = 5.43 LKK3148 pKa = 10.85 LPDD3151 pKa = 3.61 AEE3153 pKa = 5.06 IDD3155 pKa = 3.28 TSTYY3159 pKa = 9.09 SVKK3162 pKa = 9.92 YY3163 pKa = 8.46 WKK3165 pKa = 9.88 IVQCISCIGQLPINDD3180 pKa = 3.39 FDD3182 pKa = 4.87 KK3183 pKa = 11.2 RR3184 pKa = 11.84 DD3185 pKa = 3.54 ILFGFMNWKK3194 pKa = 9.45 VNNAGDD3200 pKa = 4.05 VNCPISKK3207 pKa = 10.19 EE3208 pKa = 4.17 EE3209 pKa = 3.87 NSVLSEE3215 pKa = 4.4 FEE3217 pKa = 4.03 GQTILHH3223 pKa = 5.63 VLASEE3228 pKa = 4.05 LHH3230 pKa = 6.23 LIKK3233 pKa = 10.36 DD3234 pKa = 3.52 TSEE3237 pKa = 4.23 RR3238 pKa = 11.84 EE3239 pKa = 4.02 ALVNLVDD3246 pKa = 4.49 YY3247 pKa = 10.75 INSPSEE3253 pKa = 3.91 LVKK3256 pKa = 10.66 KK3257 pKa = 10.48 KK3258 pKa = 10.3 PYY3260 pKa = 10.56 LGTTASFRR3268 pKa = 11.84 TWGSGNRR3275 pKa = 11.84 DD3276 pKa = 2.72 GRR3278 pKa = 11.84 FTYY3281 pKa = 10.32 SSRR3284 pKa = 11.84 SGEE3287 pKa = 3.78 ATGIFVGTKK3296 pKa = 8.63 LHH3298 pKa = 6.94 IYY3300 pKa = 9.98 LSNDD3304 pKa = 2.88 ALSLLDD3310 pKa = 3.55 EE3311 pKa = 4.58 VEE3313 pKa = 4.89 RR3314 pKa = 11.84 NVQGWLSQRR3323 pKa = 11.84 RR3324 pKa = 11.84 TEE3326 pKa = 3.91 IFTVEE3331 pKa = 3.61 QHH3333 pKa = 5.62 EE3334 pKa = 4.27 AFINLLPSLSEE3345 pKa = 4.12 FGSKK3349 pKa = 10.47 GSEE3352 pKa = 3.79 GKK3354 pKa = 9.89 ILSVVVDD3361 pKa = 4.3 RR3362 pKa = 11.84 KK3363 pKa = 10.53 NPRR3366 pKa = 11.84 FLKK3369 pKa = 9.83 YY3370 pKa = 9.26 TDD3372 pKa = 3.41 PKK3374 pKa = 10.95 KK3375 pKa = 10.18 NAKK3378 pKa = 8.78 SHH3380 pKa = 4.83 IVKK3383 pKa = 9.88 VKK3385 pKa = 10.35 KK3386 pKa = 10.09 HH3387 pKa = 5.48 ILTVKK3392 pKa = 10.2 KK3393 pKa = 9.67 INHH3396 pKa = 5.69 VEE3398 pKa = 4.12 FEE3400 pKa = 4.42 SDD3402 pKa = 5.09 PKK3404 pKa = 11.21 LLWSKK3409 pKa = 10.88 SGLSIVFDD3417 pKa = 4.11 EE3418 pKa = 4.53 VATDD3422 pKa = 3.21 ITYY3425 pKa = 10.31 HH3426 pKa = 5.92 EE3427 pKa = 4.68 RR3428 pKa = 11.84 LSIIRR3433 pKa = 11.84 NMLSGVVGEE3442 pKa = 5.17 RR3443 pKa = 11.84 PLQSFFQDD3451 pKa = 3.44 TQIVLSKK3458 pKa = 10.95 LKK3460 pKa = 10.77 FSDD3463 pKa = 4.13 TILMNSIALLHH3474 pKa = 6.69 SYY3476 pKa = 10.23 LLHH3479 pKa = 7.19 APLDD3483 pKa = 4.03 AFNKK3487 pKa = 10.22 LGTKK3491 pKa = 7.86 EE3492 pKa = 4.29 TVLRR3496 pKa = 11.84 TYY3498 pKa = 11.07 LKK3500 pKa = 10.36 DD3501 pKa = 3.37 KK3502 pKa = 11.02 QVIQLNCSIQEE3513 pKa = 4.41 KK3514 pKa = 10.05 ILSSEE3519 pKa = 3.75 KK3520 pKa = 11.04 LMFTKK3525 pKa = 7.73 TTPYY3529 pKa = 10.64 EE3530 pKa = 4.04 SDD3532 pKa = 3.45 STVLSSVSKK3541 pKa = 10.58 VLSEE3545 pKa = 3.99 NMLPIDD3551 pKa = 3.53 SWPEE3555 pKa = 3.56 VQEE3558 pKa = 3.85 QLEE3561 pKa = 4.25 AQGLSNFLLNFKK3573 pKa = 10.22 PEE3575 pKa = 3.99 PTKK3578 pKa = 10.89 GYY3580 pKa = 10.8 LMWNLQPNITVNKK3593 pKa = 10.07 LVISDD3598 pKa = 3.48 IKK3600 pKa = 11.2 DD3601 pKa = 3.41 VVSSVDD3607 pKa = 3.33 SGVLVPAFLAFLFNPSLLKK3626 pKa = 10.63 EE3627 pKa = 4.14 LTSVSSKK3634 pKa = 10.94 ALSALSSLKK3643 pKa = 10.48 INSEE3647 pKa = 3.75 QVDD3650 pKa = 3.68 RR3651 pKa = 11.84 VIIATLYY3658 pKa = 10.39 CFQTEE3663 pKa = 4.14 KK3664 pKa = 10.65 PVRR3667 pKa = 11.84 KK3668 pKa = 9.69 GLLFKK3673 pKa = 10.88 SGSLLSLCRR3682 pKa = 11.84 KK3683 pKa = 7.94 TSFKK3687 pKa = 10.0 IGSRR3691 pKa = 11.84 IEE3693 pKa = 3.91 VTPVSNLDD3701 pKa = 3.64 EE3702 pKa = 4.31 VDD3704 pKa = 3.55 LTFKK3708 pKa = 10.38 IRR3710 pKa = 11.84 CADD3713 pKa = 3.7 PQDD3716 pKa = 3.78 SLLSKK3721 pKa = 10.09 EE3722 pKa = 4.12 EE3723 pKa = 3.89 KK3724 pKa = 9.32 KK3725 pKa = 11.09 AKK3727 pKa = 9.43 IVRR3730 pKa = 11.84 NFEE3733 pKa = 3.6 NSLRR3737 pKa = 11.84 GILFDD3742 pKa = 4.17 YY3743 pKa = 10.9 ISMKK3747 pKa = 9.03 EE3748 pKa = 3.97 ARR3750 pKa = 11.84 NVFPDD3755 pKa = 3.94 FRR3757 pKa = 11.84 LEE3759 pKa = 4.06 LDD3761 pKa = 3.37 PTGTKK3766 pKa = 10.11 LAFTARR3772 pKa = 11.84 PWDD3775 pKa = 3.64 NKK3777 pKa = 10.49 KK3778 pKa = 10.37 FDD3780 pKa = 3.94 YY3781 pKa = 10.53 LGLMFEE3787 pKa = 4.72 GKK3789 pKa = 9.8 DD3790 pKa = 3.8 RR3791 pKa = 11.84 NDD3793 pKa = 3.42 EE3794 pKa = 4.11 CDD3796 pKa = 4.24 LIANLVLFLLGCKK3809 pKa = 9.8 YY3810 pKa = 10.15 EE3811 pKa = 4.26 EE3812 pKa = 4.09 KK3813 pKa = 9.91 TAVVNTNADD3822 pKa = 3.22 NDD3824 pKa = 3.45 ISLDD3828 pKa = 3.7 FLTDD3832 pKa = 3.57 DD3833 pKa = 4.93 FGLQEE3838 pKa = 4.44 SDD3840 pKa = 3.96 LEE3842 pKa = 4.07 ASRR3845 pKa = 11.84 RR3846 pKa = 11.84 DD3847 pKa = 3.3 SHH3849 pKa = 6.79 PNSTGFFSDD3858 pKa = 4.73 DD3859 pKa = 3.29 EE3860 pKa = 4.58 YY3861 pKa = 11.98
Molecular weight: 440.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.99
IPC2_protein 7.468
IPC_protein 7.22
Toseland 6.927
ProMoST 7.892
Dawson 7.98
Bjellqvist 8.404
Wikipedia 7.892
Rodwell 8.024
Grimsley 7.0
Solomon 7.995
Lehninger 8.009
Nozaki 8.639
DTASelect 8.112
Thurlkill 8.185
EMBOSS 8.185
Sillero 8.507
Patrickios 4.444
IPC_peptide 7.98
IPC2_peptide 7.278
IPC2.peptide.svr19 7.458
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
5815
673
3861
1938.3
219.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.712 ± 1.027
2.562 ± 0.876
5.211 ± 0.318
6.466 ± 0.458
4.557 ± 0.095
5.262 ± 1.044
2.184 ± 0.261
6.346 ± 0.658
7.842 ± 0.325
10.61 ± 1.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.098 ± 0.239
4.764 ± 0.533
3.371 ± 0.703
3.457 ± 1.024
4.557 ± 0.496
8.753 ± 0.715
6.449 ± 0.775
6.552 ± 0.52
1.359 ± 0.367
2.889 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here