Prevotellaceae bacterium HUN156

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; unclassified Prevotellaceae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1K1M804|A0A1K1M804_9BACT BadF-type ATPase OS=Prevotellaceae bacterium HUN156 OX=1520830 GN=SAMN02910409_0807 PE=4 SV=1
MM1 pKa = 6.97NTTIVIYY8 pKa = 10.39GSSTGTCEE16 pKa = 5.07AIAEE20 pKa = 4.43KK21 pKa = 10.18IAQKK25 pKa = 10.52LGCEE29 pKa = 4.17VMNVQGLSADD39 pKa = 3.6VVNNHH44 pKa = 5.71QNLILGTSTWGAGEE58 pKa = 4.8LQDD61 pKa = 5.13DD62 pKa = 4.56WYY64 pKa = 10.8DD65 pKa = 3.61GLKK68 pKa = 10.23VLQGAEE74 pKa = 4.11LSGKK78 pKa = 7.8TFALFGCGDD87 pKa = 3.92CEE89 pKa = 4.87SYY91 pKa = 11.74GDD93 pKa = 3.93TFVGGLGEE101 pKa = 4.89LYY103 pKa = 10.77NGIKK107 pKa = 10.49GCGAKK112 pKa = 9.77IVGAVEE118 pKa = 3.84TDD120 pKa = 3.42GYY122 pKa = 9.9TFDD125 pKa = 5.06DD126 pKa = 4.05SEE128 pKa = 4.57AVVDD132 pKa = 4.58GKK134 pKa = 11.06FVGLPLDD141 pKa = 5.93DD142 pKa = 4.75INEE145 pKa = 4.26DD146 pKa = 3.77DD147 pKa = 4.02KK148 pKa = 11.64TDD150 pKa = 3.34TRR152 pKa = 11.84IEE154 pKa = 3.67AWIADD159 pKa = 4.06ILPDD163 pKa = 4.12LL164 pKa = 4.59

Molecular weight: 17.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1K1P363|A0A1K1P363_9BACT Uncharacterized protein OS=Prevotellaceae bacterium HUN156 OX=1520830 GN=SAMN02910409_2199 PE=4 SV=1
MM1 pKa = 7.85PNGKK5 pKa = 9.19KK6 pKa = 10.25KK7 pKa = 10.12KK8 pKa = 7.0GHH10 pKa = 6.14KK11 pKa = 9.06MATHH15 pKa = 6.13KK16 pKa = 10.39RR17 pKa = 11.84KK18 pKa = 9.84KK19 pKa = 9.28RR20 pKa = 11.84LRR22 pKa = 11.84KK23 pKa = 9.25NRR25 pKa = 11.84HH26 pKa = 4.69KK27 pKa = 11.1SKK29 pKa = 11.1

Molecular weight: 3.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2311

0

2311

842443

29

2448

364.5

41.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.407 ± 0.052

1.333 ± 0.017

5.819 ± 0.035

6.511 ± 0.054

4.217 ± 0.036

6.846 ± 0.051

2.091 ± 0.023

6.648 ± 0.035

6.336 ± 0.043

8.733 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.996 ± 0.025

4.947 ± 0.044

3.679 ± 0.027

3.946 ± 0.035

4.562 ± 0.041

5.827 ± 0.043

5.871 ± 0.056

6.569 ± 0.036

1.306 ± 0.021

4.358 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski