Prevotellaceae bacterium HUN156
Average proteome isoelectric point is 6.2
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2311 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1K1M804|A0A1K1M804_9BACT BadF-type ATPase OS=Prevotellaceae bacterium HUN156 OX=1520830 GN=SAMN02910409_0807 PE=4 SV=1
MM1 pKa = 6.97 NTTIVIYY8 pKa = 10.39 GSSTGTCEE16 pKa = 5.07 AIAEE20 pKa = 4.43 KK21 pKa = 10.18 IAQKK25 pKa = 10.52 LGCEE29 pKa = 4.17 VMNVQGLSADD39 pKa = 3.6 VVNNHH44 pKa = 5.71 QNLILGTSTWGAGEE58 pKa = 4.8 LQDD61 pKa = 5.13 DD62 pKa = 4.56 WYY64 pKa = 10.8 DD65 pKa = 3.61 GLKK68 pKa = 10.23 VLQGAEE74 pKa = 4.11 LSGKK78 pKa = 7.8 TFALFGCGDD87 pKa = 3.92 CEE89 pKa = 4.87 SYY91 pKa = 11.74 GDD93 pKa = 3.93 TFVGGLGEE101 pKa = 4.89 LYY103 pKa = 10.77 NGIKK107 pKa = 10.49 GCGAKK112 pKa = 9.77 IVGAVEE118 pKa = 3.84 TDD120 pKa = 3.42 GYY122 pKa = 9.9 TFDD125 pKa = 5.06 DD126 pKa = 4.05 SEE128 pKa = 4.57 AVVDD132 pKa = 4.58 GKK134 pKa = 11.06 FVGLPLDD141 pKa = 5.93 DD142 pKa = 4.75 INEE145 pKa = 4.26 DD146 pKa = 3.77 DD147 pKa = 4.02 KK148 pKa = 11.64 TDD150 pKa = 3.34 TRR152 pKa = 11.84 IEE154 pKa = 3.67 AWIADD159 pKa = 4.06 ILPDD163 pKa = 4.12 LL164 pKa = 4.59
Molecular weight: 17.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.821
IPC_protein 3.808
Toseland 3.592
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.503
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.151
Thurlkill 3.656
EMBOSS 3.745
Sillero 3.935
Patrickios 1.888
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|A0A1K1P363|A0A1K1P363_9BACT Uncharacterized protein OS=Prevotellaceae bacterium HUN156 OX=1520830 GN=SAMN02910409_2199 PE=4 SV=1
MM1 pKa = 7.85 PNGKK5 pKa = 9.19 KK6 pKa = 10.25 KK7 pKa = 10.12 KK8 pKa = 7.0 GHH10 pKa = 6.14 KK11 pKa = 9.06 MATHH15 pKa = 6.13 KK16 pKa = 10.39 RR17 pKa = 11.84 KK18 pKa = 9.84 KK19 pKa = 9.28 RR20 pKa = 11.84 LRR22 pKa = 11.84 KK23 pKa = 9.25 NRR25 pKa = 11.84 HH26 pKa = 4.69 KK27 pKa = 11.1 SKK29 pKa = 11.1
Molecular weight: 3.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.361
IPC2_protein 10.862
IPC_protein 12.325
Toseland 12.53
ProMoST 12.983
Dawson 12.544
Bjellqvist 12.501
Wikipedia 12.983
Rodwell 12.647
Grimsley 12.574
Solomon 12.998
Lehninger 12.91
Nozaki 12.53
DTASelect 12.501
Thurlkill 12.53
EMBOSS 13.013
Sillero 12.53
Patrickios 12.369
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.904
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2311
0
2311
842443
29
2448
364.5
41.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.407 ± 0.052
1.333 ± 0.017
5.819 ± 0.035
6.511 ± 0.054
4.217 ± 0.036
6.846 ± 0.051
2.091 ± 0.023
6.648 ± 0.035
6.336 ± 0.043
8.733 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.996 ± 0.025
4.947 ± 0.044
3.679 ± 0.027
3.946 ± 0.035
4.562 ± 0.041
5.827 ± 0.043
5.871 ± 0.056
6.569 ± 0.036
1.306 ± 0.021
4.358 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here