Amino acid dipepetide frequency for Bacillus sp. V3-13

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.359AlaAla: 7.359 ± 0.108	0.66AlaCys: 0.66 ± 0.024	3.882AlaAsp: 3.882 ± 0.066	5.467AlaGlu: 5.467 ± 0.077	3.451AlaPhe: 3.451 ± 0.053	6.364AlaGly: 6.364 ± 0.084	1.346AlaHis: 1.346 ± 0.04	6.035AlaIle: 6.035 ± 0.074	4.783AlaLys: 4.783 ± 0.071	7.458AlaLeu: 7.458 ± 0.081	1.987AlaMet: 1.987 ± 0.046	2.976AlaAsn: 2.976 ± 0.052	2.192AlaPro: 2.192 ± 0.041	2.114AlaGln: 2.114 ± 0.04	3.09AlaArg: 3.09 ± 0.049	4.229AlaSer: 4.229 ± 0.062	3.269AlaThr: 3.269 ± 0.054	6.007AlaVal: 6.007 ± 0.078	0.63AlaTrp: 0.63 ± 0.024	2.323AlaTyr: 2.323 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.478CysAla: 0.478 ± 0.019	0.101CysCys: 0.101 ± 0.01	0.388CysAsp: 0.388 ± 0.018	0.474CysGlu: 0.474 ± 0.022	0.318CysPhe: 0.318 ± 0.013	0.678CysGly: 0.678 ± 0.022	0.232CysHis: 0.232 ± 0.014	0.518CysIle: 0.518 ± 0.022	0.378CysLys: 0.378 ± 0.018	0.727CysLeu: 0.727 ± 0.027	0.181CysMet: 0.181 ± 0.012	0.332CysAsn: 0.332 ± 0.019	0.395CysPro: 0.395 ± 0.023	0.229CysGln: 0.229 ± 0.013	0.333CysArg: 0.333 ± 0.016	0.539CysSer: 0.539 ± 0.023	0.398CysThr: 0.398 ± 0.018	0.406CysVal: 0.406 ± 0.019	0.072CysTrp: 0.072 ± 0.007	0.268CysTyr: 0.268 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	3.271AspAla: 3.271 ± 0.064	0.383AspCys: 0.383 ± 0.019	2.47AspAsp: 2.47 ± 0.051	4.297AspGlu: 4.297 ± 0.064	2.415AspPhe: 2.415 ± 0.045	3.483AspGly: 3.483 ± 0.057	1.202AspHis: 1.202 ± 0.031	4.138AspIle: 4.138 ± 0.059	3.003AspLys: 3.003 ± 0.054	5.027AspLeu: 5.027 ± 0.067	1.226AspMet: 1.226 ± 0.034	1.78AspAsn: 1.78 ± 0.037	2.246AspPro: 2.246 ± 0.047	1.985AspGln: 1.985 ± 0.038	2.473AspArg: 2.473 ± 0.044	2.59AspSer: 2.59 ± 0.046	2.199AspThr: 2.199 ± 0.041	3.414AspVal: 3.414 ± 0.061	0.656AspTrp: 0.656 ± 0.022	2.086AspTyr: 2.086 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	5.87GluAla: 5.87 ± 0.082	0.387GluCys: 0.387 ± 0.018	3.686GluAsp: 3.686 ± 0.062	6.569GluGlu: 6.569 ± 0.096	2.651GluPhe: 2.651 ± 0.047	4.272GluGly: 4.272 ± 0.069	1.495GluHis: 1.495 ± 0.034	5.872GluIle: 5.872 ± 0.08	6.612GluLys: 6.612 ± 0.079	7.336GluLeu: 7.336 ± 0.096	2.268GluMet: 2.268 ± 0.045	3.706GluAsn: 3.706 ± 0.055	2.13GluPro: 2.13 ± 0.041	3.464GluGln: 3.464 ± 0.067	3.527GluArg: 3.527 ± 0.064	3.353GluSer: 3.353 ± 0.049	3.837GluThr: 3.837 ± 0.059	4.817GluVal: 4.817 ± 0.067	0.815GluTrp: 0.815 ± 0.026	2.26GluTyr: 2.26 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	3.305PheAla: 3.305 ± 0.054	0.375PheCys: 0.375 ± 0.018	2.291PheAsp: 2.291 ± 0.047	2.925PheGlu: 2.925 ± 0.051	2.435PhePhe: 2.435 ± 0.059	3.376PheGly: 3.376 ± 0.062	0.974PheHis: 0.974 ± 0.028	3.828PheIle: 3.828 ± 0.069	2.355PheLys: 2.355 ± 0.045	4.699PheLeu: 4.699 ± 0.079	1.176PheMet: 1.176 ± 0.034	1.88PheAsn: 1.88 ± 0.04	1.834PhePro: 1.834 ± 0.042	1.598PheGln: 1.598 ± 0.036	1.666PheArg: 1.666 ± 0.036	3.255PheSer: 3.255 ± 0.057	2.484PheThr: 2.484 ± 0.044	2.998PheVal: 2.998 ± 0.05	0.511PheTrp: 0.511 ± 0.023	1.736PheTyr: 1.736 ± 0.041	0.0PheXaa: 0.0 ± 0.0
Gly	5.299GlyAla: 5.299 ± 0.091	0.649GlyCys: 0.649 ± 0.024	3.254GlyAsp: 3.254 ± 0.049	4.721GlyGlu: 4.721 ± 0.061	3.584GlyPhe: 3.584 ± 0.063	5.03GlyGly: 5.03 ± 0.082	1.444GlyHis: 1.444 ± 0.035	6.177GlyIle: 6.177 ± 0.073	5.272GlyLys: 5.272 ± 0.066	6.901GlyLeu: 6.901 ± 0.082	2.108GlyMet: 2.108 ± 0.046	2.931GlyAsn: 2.931 ± 0.065	1.932GlyPro: 1.932 ± 0.043	2.352GlyGln: 2.352 ± 0.046	2.867GlyArg: 2.867 ± 0.054	4.204GlySer: 4.204 ± 0.065	4.114GlyThr: 4.114 ± 0.064	5.011GlyVal: 5.011 ± 0.065	0.833GlyTrp: 0.833 ± 0.032	2.801GlyTyr: 2.801 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	1.38HisAla: 1.38 ± 0.032	0.223HisCys: 0.223 ± 0.014	1.038HisAsp: 1.038 ± 0.029	1.456HisGlu: 1.456 ± 0.036	1.038HisPhe: 1.038 ± 0.029	1.453HisGly: 1.453 ± 0.041	0.645HisHis: 0.645 ± 0.025	1.44HisIle: 1.44 ± 0.036	1.034HisLys: 1.034 ± 0.029	2.084HisLeu: 2.084 ± 0.049	0.5HisMet: 0.5 ± 0.02	0.821HisAsn: 0.821 ± 0.028	1.102HisPro: 1.102 ± 0.034	0.79HisGln: 0.79 ± 0.022	0.936HisArg: 0.936 ± 0.025	1.243HisSer: 1.243 ± 0.031	0.947HisThr: 0.947 ± 0.025	1.368HisVal: 1.368 ± 0.032	0.227HisTrp: 0.227 ± 0.015	0.885HisTyr: 0.885 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.224IleAla: 6.224 ± 0.079	0.665IleCys: 0.665 ± 0.023	4.385IleAsp: 4.385 ± 0.055	5.787IleGlu: 5.787 ± 0.078	3.373IlePhe: 3.373 ± 0.059	6.031IleGly: 6.031 ± 0.081	1.672IleHis: 1.672 ± 0.037	5.821IleIle: 5.821 ± 0.083	4.713IleLys: 4.713 ± 0.071	7.167IleLeu: 7.167 ± 0.097	1.75IleMet: 1.75 ± 0.042	3.422IleAsn: 3.422 ± 0.053	3.451IlePro: 3.451 ± 0.048	2.686IleGln: 2.686 ± 0.049	3.274IleArg: 3.274 ± 0.053	5.129IleSer: 5.129 ± 0.063	4.214IleThr: 4.214 ± 0.058	5.531IleVal: 5.531 ± 0.068	0.622IleTrp: 0.622 ± 0.021	2.409IleTyr: 2.409 ± 0.05	0.0IleXaa: 0.0 ± 0.0
Lys	4.945LysAla: 4.945 ± 0.076	0.345LysCys: 0.345 ± 0.018	3.559LysAsp: 3.559 ± 0.058	6.377LysGlu: 6.377 ± 0.082	1.976LysPhe: 1.976 ± 0.041	4.5LysGly: 4.5 ± 0.053	1.269LysHis: 1.269 ± 0.032	4.91LysIle: 4.91 ± 0.062	5.495LysLys: 5.495 ± 0.082	5.996LysLeu: 5.996 ± 0.078	2.04LysMet: 2.04 ± 0.036	3.323LysAsn: 3.323 ± 0.055	2.267LysPro: 2.267 ± 0.048	2.998LysGln: 2.998 ± 0.048	3.163LysArg: 3.163 ± 0.051	3.323LysSer: 3.323 ± 0.051	3.56LysThr: 3.56 ± 0.056	4.435LysVal: 4.435 ± 0.063	0.768LysTrp: 0.768 ± 0.028	2.195LysTyr: 2.195 ± 0.041	0.0LysXaa: 0.0 ± 0.0
Leu	8.02LeuAla: 8.02 ± 0.098	0.654LeuCys: 0.654 ± 0.021	4.829LeuAsp: 4.829 ± 0.066	6.753LeuGlu: 6.753 ± 0.087	4.817LeuPhe: 4.817 ± 0.085	6.615LeuGly: 6.615 ± 0.082	1.925LeuHis: 1.925 ± 0.04	7.359LeuIle: 7.359 ± 0.101	6.683LeuLys: 6.683 ± 0.083	10.232LeuLeu: 10.232 ± 0.12	2.446LeuMet: 2.446 ± 0.048	4.382LeuAsn: 4.382 ± 0.067	4.008LeuPro: 4.008 ± 0.056	3.541LeuGln: 3.541 ± 0.055	3.794LeuArg: 3.794 ± 0.06	6.594LeuSer: 6.594 ± 0.08	5.627LeuThr: 5.627 ± 0.063	6.249LeuVal: 6.249 ± 0.078	0.832LeuTrp: 0.832 ± 0.027	3.119LeuTyr: 3.119 ± 0.056	0.0LeuXaa: 0.0 ± 0.0
Met	2.134MetAla: 2.134 ± 0.043	0.138MetCys: 0.138 ± 0.01	1.315MetAsp: 1.315 ± 0.035	1.872MetGlu: 1.872 ± 0.039	1.011MetPhe: 1.011 ± 0.033	1.725MetGly: 1.725 ± 0.038	0.417MetHis: 0.417 ± 0.022	2.134MetIle: 2.134 ± 0.044	2.337MetLys: 2.337 ± 0.047	2.492MetLeu: 2.492 ± 0.043	0.777MetMet: 0.777 ± 0.024	1.455MetAsn: 1.455 ± 0.039	1.044MetPro: 1.044 ± 0.033	0.809MetGln: 0.809 ± 0.025	1.121MetArg: 1.121 ± 0.03	1.521MetSer: 1.521 ± 0.04	1.463MetThr: 1.463 ± 0.04	1.698MetVal: 1.698 ± 0.035	0.187MetTrp: 0.187 ± 0.013	0.693MetTyr: 0.693 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	2.789AsnAla: 2.789 ± 0.054	0.335AsnCys: 0.335 ± 0.018	2.186AsnAsp: 2.186 ± 0.04	3.524AsnGlu: 3.524 ± 0.056	1.643AsnPhe: 1.643 ± 0.037	3.467AsnGly: 3.467 ± 0.072	0.987AsnHis: 0.987 ± 0.028	3.495AsnIle: 3.495 ± 0.061	2.812AsnLys: 2.812 ± 0.056	3.896AsnLeu: 3.896 ± 0.063	1.118AsnMet: 1.118 ± 0.029	1.953AsnAsn: 1.953 ± 0.054	2.24AsnPro: 2.24 ± 0.043	1.751AsnGln: 1.751 ± 0.043	2.134AsnArg: 2.134 ± 0.038	2.389AsnSer: 2.389 ± 0.041	2.081AsnThr: 2.081 ± 0.04	2.899AsnVal: 2.899 ± 0.043	0.546AsnTrp: 0.546 ± 0.02	1.463AsnTyr: 1.463 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	2.943ProAla: 2.943 ± 0.05	0.234ProCys: 0.234 ± 0.014	2.291ProAsp: 2.291 ± 0.042	3.288ProGlu: 3.288 ± 0.049	2.082ProPhe: 2.082 ± 0.042	2.873ProGly: 2.873 ± 0.058	0.873ProHis: 0.873 ± 0.027	2.696ProIle: 2.696 ± 0.049	2.089ProLys: 2.089 ± 0.041	3.71ProLeu: 3.71 ± 0.056	0.769ProMet: 0.769 ± 0.026	1.618ProAsn: 1.618 ± 0.04	1.143ProPro: 1.143 ± 0.033	1.109ProGln: 1.109 ± 0.031	1.227ProArg: 1.227 ± 0.03	2.111ProSer: 2.111 ± 0.042	1.716ProThr: 1.716 ± 0.036	3.124ProVal: 3.124 ± 0.048	0.363ProTrp: 0.363 ± 0.017	1.405ProTyr: 1.405 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	2.791GlnAla: 2.791 ± 0.052	0.216GlnCys: 0.216 ± 0.014	1.507GlnAsp: 1.507 ± 0.04	2.763GlnGlu: 2.763 ± 0.051	1.685GlnPhe: 1.685 ± 0.04	2.099GlnGly: 2.099 ± 0.048	0.738GlnHis: 0.738 ± 0.026	2.628GlnIle: 2.628 ± 0.045	2.591GlnLys: 2.591 ± 0.045	3.976GlnLeu: 3.976 ± 0.059	1.073GlnMet: 1.073 ± 0.034	1.637GlnAsn: 1.637 ± 0.04	1.255GlnPro: 1.255 ± 0.033	1.79GlnGln: 1.79 ± 0.046	1.5GlnArg: 1.5 ± 0.037	2.015GlnSer: 2.015 ± 0.046	1.946GlnThr: 1.946 ± 0.04	2.219GlnVal: 2.219 ± 0.044	0.362GlnTrp: 0.362 ± 0.019	1.324GlnTyr: 1.324 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.749ArgAla: 2.749 ± 0.052	0.276ArgCys: 0.276 ± 0.016	2.125ArgAsp: 2.125 ± 0.043	3.448ArgGlu: 3.448 ± 0.063	2.068ArgPhe: 2.068 ± 0.04	2.475ArgGly: 2.475 ± 0.047	0.835ArgHis: 0.835 ± 0.029	3.28ArgIle: 3.28 ± 0.048	3.361ArgLys: 3.361 ± 0.056	4.278ArgLeu: 4.278 ± 0.066	1.222ArgMet: 1.222 ± 0.029	2.025ArgAsn: 2.025 ± 0.042	1.461ArgPro: 1.461 ± 0.036	1.687ArgGln: 1.687 ± 0.035	1.993ArgArg: 1.993 ± 0.047	2.305ArgSer: 2.305 ± 0.04	2.127ArgThr: 2.127 ± 0.041	2.607ArgVal: 2.607 ± 0.049	0.423ArgTrp: 0.423 ± 0.02	1.543ArgTyr: 1.543 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	4.069SerAla: 4.069 ± 0.059	0.464SerCys: 0.464 ± 0.016	2.649SerAsp: 2.649 ± 0.044	3.862SerGlu: 3.862 ± 0.059	3.27SerPhe: 3.27 ± 0.055	4.735SerGly: 4.735 ± 0.072	1.192SerHis: 1.192 ± 0.03	4.794SerIle: 4.794 ± 0.07	3.464SerLys: 3.464 ± 0.06	6.25SerLeu: 6.25 ± 0.079	1.636SerMet: 1.636 ± 0.031	2.276SerAsn: 2.276 ± 0.043	2.261SerPro: 2.261 ± 0.043	1.854SerGln: 1.854 ± 0.041	2.536SerArg: 2.536 ± 0.039	3.639SerSer: 3.639 ± 0.057	2.932SerThr: 2.932 ± 0.05	4.036SerVal: 4.036 ± 0.061	0.664SerTrp: 0.664 ± 0.025	2.108SerTyr: 2.108 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	4.259ThrAla: 4.259 ± 0.062	0.344ThrCys: 0.344 ± 0.018	2.617ThrAsp: 2.617 ± 0.052	3.575ThrGlu: 3.575 ± 0.054	2.407ThrPhe: 2.407 ± 0.051	4.475ThrGly: 4.475 ± 0.061	0.973ThrHis: 0.973 ± 0.028	4.342ThrIle: 4.342 ± 0.063	3.095ThrLys: 3.095 ± 0.051	4.834ThrLeu: 4.834 ± 0.061	1.247ThrMet: 1.247 ± 0.031	2.176ThrAsn: 2.176 ± 0.041	2.153ThrPro: 2.153 ± 0.045	1.213ThrGln: 1.213 ± 0.032	1.912ThrArg: 1.912 ± 0.044	2.976ThrSer: 2.976 ± 0.052	2.641ThrThr: 2.641 ± 0.056	4.262ThrVal: 4.262 ± 0.062	0.493ThrTrp: 0.493 ± 0.02	1.733ThrTyr: 1.733 ± 0.046	0.0ThrXaa: 0.0 ± 0.0
Val	5.006ValAla: 5.006 ± 0.066	0.612ValCys: 0.612 ± 0.025	3.467ValAsp: 3.467 ± 0.05	4.571ValGlu: 4.571 ± 0.067	3.243ValPhe: 3.243 ± 0.054	4.53ValGly: 4.53 ± 0.061	1.365ValHis: 1.365 ± 0.034	5.657ValIle: 5.657 ± 0.07	4.591ValLys: 4.591 ± 0.068	6.768ValLeu: 6.768 ± 0.088	1.719ValMet: 1.719 ± 0.039	3.058ValAsn: 3.058 ± 0.052	2.815ValPro: 2.815 ± 0.056	2.265ValGln: 2.265 ± 0.042	2.632ValArg: 2.632 ± 0.043	4.584ValSer: 4.584 ± 0.067	3.93ValThr: 3.93 ± 0.068	4.728ValVal: 4.728 ± 0.072	0.657ValTrp: 0.657 ± 0.022	2.3ValTyr: 2.3 ± 0.044	0.0ValXaa: 0.0 ± 0.0
Trp	0.64TrpAla: 0.64 ± 0.022	0.081TrpCys: 0.081 ± 0.009	0.524TrpAsp: 0.524 ± 0.022	0.671TrpGlu: 0.671 ± 0.023	0.486TrpPhe: 0.486 ± 0.021	0.711TrpGly: 0.711 ± 0.026	0.196TrpHis: 0.196 ± 0.013	0.796TrpIle: 0.796 ± 0.025	0.72TrpLys: 0.72 ± 0.023	1.183TrpLeu: 1.183 ± 0.034	0.31TrpMet: 0.31 ± 0.015	0.556TrpAsn: 0.556 ± 0.021	0.288TrpPro: 0.288 ± 0.015	0.367TrpGln: 0.367 ± 0.017	0.438TrpArg: 0.438 ± 0.017	0.585TrpSer: 0.585 ± 0.024	0.518TrpThr: 0.518 ± 0.02	0.63TrpVal: 0.63 ± 0.023	0.148TrpTrp: 0.148 ± 0.012	0.342TrpTyr: 0.342 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.089TyrAla: 2.089 ± 0.043	0.303TyrCys: 0.303 ± 0.016	1.882TyrAsp: 1.882 ± 0.063	2.525TyrGlu: 2.525 ± 0.045	1.749TyrPhe: 1.749 ± 0.043	2.592TyrGly: 2.592 ± 0.05	0.832TyrHis: 0.832 ± 0.026	2.405TyrIle: 2.405 ± 0.049	2.058TyrLys: 2.058 ± 0.042	3.467TyrLeu: 3.467 ± 0.058	0.789TyrMet: 0.789 ± 0.022	1.4TyrAsn: 1.4 ± 0.032	1.446TyrPro: 1.446 ± 0.037	1.515TyrGln: 1.515 ± 0.034	1.668TyrArg: 1.668 ± 0.037	2.11TyrSer: 2.11 ± 0.046	1.722TyrThr: 1.722 ± 0.038	2.06TyrVal: 2.06 ± 0.045	0.38TyrTrp: 0.38 ± 0.018	1.396TyrTyr: 1.396 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4299 proteins (1262598 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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