Streptococcus satellite phage Javan96
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZXV3|A0A4D5ZXV3_9VIRU ATP-dependent DNA helicase OS=Streptococcus satellite phage Javan96 OX=2558868 GN=JavanS96_0002 PE=3 SV=1
MM1 pKa = 7.55 RR2 pKa = 11.84 TFSDD6 pKa = 3.67 TPKK9 pKa = 10.21 TFTFHH14 pKa = 5.7 YY15 pKa = 8.55 TFKK18 pKa = 11.1 DD19 pKa = 3.09 FDD21 pKa = 4.09 TAQVACHH28 pKa = 6.88 AILGYY33 pKa = 7.77 MTGTYY38 pKa = 7.11 EE39 pKa = 4.76 QPVIDD44 pKa = 3.97 ATYY47 pKa = 10.82 HH48 pKa = 6.24 NDD50 pKa = 3.59 DD51 pKa = 3.72 QGGHH55 pKa = 6.59 ANQLVLEE62 pKa = 4.2 YY63 pKa = 11.27 AEE65 pKa = 4.49 DD66 pKa = 3.69 RR67 pKa = 11.84 KK68 pKa = 10.66 LSKK71 pKa = 10.32 VFKK74 pKa = 10.15 RR75 pKa = 11.84 ICDD78 pKa = 3.69 SFKK81 pKa = 10.93 DD82 pKa = 4.21 YY83 pKa = 11.52 YY84 pKa = 10.51 NQPEE88 pKa = 4.58 DD89 pKa = 3.61 MTDD92 pKa = 3.66 DD93 pKa = 4.18 EE94 pKa = 5.61 LDD96 pKa = 3.47 NMAQEE101 pKa = 4.21 IEE103 pKa = 4.08 LLKK106 pKa = 10.67 EE107 pKa = 4.01 VEE109 pKa = 4.31 EE110 pKa = 5.35 LDD112 pKa = 3.77 DD113 pKa = 3.69 QRR115 pKa = 11.84 VVSLSKK121 pKa = 9.84 STQEE125 pKa = 4.12 KK126 pKa = 10.59 VNDD129 pKa = 4.26 RR130 pKa = 11.84 DD131 pKa = 3.57 TLMTFISDD139 pKa = 3.59 HH140 pKa = 5.84 NQLAEE145 pKa = 4.25 HH146 pKa = 6.91 LSMNYY151 pKa = 10.19 KK152 pKa = 10.52 EE153 pKa = 4.5 MTPEE157 pKa = 3.92 DD158 pKa = 3.6 LGAILEE164 pKa = 4.71 SISQAFNQLYY174 pKa = 10.85 DD175 pKa = 3.68 MVVEE179 pKa = 4.31 GQLLVKK185 pKa = 10.69
Molecular weight: 21.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.341
IPC2_protein 4.355
IPC_protein 4.317
Toseland 4.139
ProMoST 4.444
Dawson 4.279
Bjellqvist 4.431
Wikipedia 4.177
Rodwell 4.151
Grimsley 4.05
Solomon 4.279
Lehninger 4.228
Nozaki 4.393
DTASelect 4.596
Thurlkill 4.151
EMBOSS 4.202
Sillero 4.431
Patrickios 3.49
IPC_peptide 4.279
IPC2_peptide 4.418
IPC2.peptide.svr19 4.353
Protein with the highest isoelectric point:
>tr|A0A4D6A270|A0A4D6A270_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan96 OX=2558868 GN=JavanS96_0011 PE=4 SV=1
MM1 pKa = 7.67 AEE3 pKa = 4.36 LWLKK7 pKa = 10.69 SYY9 pKa = 11.01 QLTVKK14 pKa = 9.5 PQTYY18 pKa = 10.31 EE19 pKa = 3.93 STKK22 pKa = 10.89 LLLKK26 pKa = 10.55 NHH28 pKa = 7.01 ILPVFGNMKK37 pKa = 10.26 LEE39 pKa = 4.9 RR40 pKa = 11.84 ITPSFVQQFANKK52 pKa = 9.47 LAHH55 pKa = 5.94 TLVNFKK61 pKa = 9.7 VACSINRR68 pKa = 11.84 RR69 pKa = 11.84 ILQYY73 pKa = 11.24 AVLLQLIPYY82 pKa = 8.96 NPARR86 pKa = 11.84 EE87 pKa = 3.87 IIIPKK92 pKa = 8.76 KK93 pKa = 9.28 QKK95 pKa = 10.4 KK96 pKa = 9.27 SADD99 pKa = 3.3 RR100 pKa = 11.84 VKK102 pKa = 10.84 FINPQNLKK110 pKa = 10.78 ALLDD114 pKa = 3.87 YY115 pKa = 10.8 MEE117 pKa = 4.77 TLAPTKK123 pKa = 9.68 YY124 pKa = 9.8 QYY126 pKa = 11.54 YY127 pKa = 8.91 YY128 pKa = 11.44 DD129 pKa = 3.68 NVLYY133 pKa = 10.56 RR134 pKa = 11.84 FLLATGCRR142 pKa = 11.84 FGEE145 pKa = 4.29 AVALEE150 pKa = 4.2 WSDD153 pKa = 3.19 IDD155 pKa = 5.0 LEE157 pKa = 5.16 AGTVSISKK165 pKa = 9.17 TYY167 pKa = 10.53 NRR169 pKa = 11.84 QINQISTPKK178 pKa = 8.61 TKK180 pKa = 10.09 SGKK183 pKa = 10.17 RR184 pKa = 11.84 IISIDD189 pKa = 3.49 NKK191 pKa = 10.74 LVLLLKK197 pKa = 10.05 QYY199 pKa = 11.05 RR200 pKa = 11.84 NRR202 pKa = 11.84 QRR204 pKa = 11.84 LIFIEE209 pKa = 4.41 IGAHH213 pKa = 4.94 APKK216 pKa = 10.57 VIFASPTLTYY226 pKa = 10.59 ASSDD230 pKa = 3.6 VRR232 pKa = 11.84 SKK234 pKa = 11.51 ALAHH238 pKa = 6.06 RR239 pKa = 11.84 CKK241 pKa = 10.54 EE242 pKa = 3.5 IGIPRR247 pKa = 11.84 FTFHH251 pKa = 7.74 AFRR254 pKa = 11.84 HH255 pKa = 4.69 THH257 pKa = 6.92 ASLLLNAGISYY268 pKa = 10.65 KK269 pKa = 10.3 EE270 pKa = 3.81 LQHH273 pKa = 7.18 RR274 pKa = 11.84 LGHH277 pKa = 5.95 SNIKK281 pKa = 7.93 MTLDD285 pKa = 3.46 TYY287 pKa = 11.1 GHH289 pKa = 6.42 ISKK292 pKa = 10.22 EE293 pKa = 4.12 KK294 pKa = 8.59 EE295 pKa = 3.91 KK296 pKa = 10.84 EE297 pKa = 3.89 AVSYY301 pKa = 10.0 FEE303 pKa = 5.0 KK304 pKa = 10.72 AINNLL309 pKa = 3.44
Molecular weight: 35.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.133
IPC2_protein 9.385
IPC_protein 9.326
Toseland 9.999
ProMoST 9.677
Dawson 10.204
Bjellqvist 9.867
Wikipedia 10.365
Rodwell 10.687
Grimsley 10.277
Solomon 10.218
Lehninger 10.189
Nozaki 9.999
DTASelect 9.867
Thurlkill 10.058
EMBOSS 10.394
Sillero 10.116
Patrickios 10.175
IPC_peptide 10.218
IPC2_peptide 8.463
IPC2.peptide.svr19 8.366
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22
0
22
3716
57
504
168.9
19.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.705 ± 0.415
0.78 ± 0.189
5.813 ± 0.465
8.073 ± 0.397
4.36 ± 0.248
4.413 ± 0.284
2.045 ± 0.251
7.481 ± 0.437
8.665 ± 0.4
10.495 ± 0.493
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.153 ± 0.266
5.84 ± 0.532
3.498 ± 0.472
4.171 ± 0.403
5.328 ± 0.276
5.274 ± 0.399
5.705 ± 0.285
4.736 ± 0.359
0.942 ± 0.153
4.521 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here