Baekduia soli
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4785 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5B8TZL5|A0A5B8TZL5_9ACTN Hydantoinase/oxoprolinase family protein OS=Baekduia soli OX=496014 GN=FSW04_00370 PE=4 SV=1
MM1 pKa = 7.16 QKK3 pKa = 9.86 WICEE7 pKa = 4.32 SCGFIYY13 pKa = 10.57 DD14 pKa = 4.34 PADD17 pKa = 3.82 GDD19 pKa = 4.11 PDD21 pKa = 4.0 GGIPPGTAFADD32 pKa = 4.06 IPDD35 pKa = 3.46 TWFCPVCGARR45 pKa = 11.84 KK46 pKa = 9.16 RR47 pKa = 11.84 DD48 pKa = 3.65 FVAYY52 pKa = 9.92 EE53 pKa = 3.93 DD54 pKa = 3.72
Molecular weight: 5.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.897
IPC_protein 3.808
Toseland 3.592
ProMoST 3.91
Dawson 3.834
Bjellqvist 4.088
Wikipedia 3.834
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.757
Nozaki 3.973
DTASelect 4.24
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.935
Patrickios 0.693
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.925
Protein with the highest isoelectric point:
>tr|A0A5B8U589|A0A5B8U589_9ACTN Glutamine synthetase OS=Baekduia soli OX=496014 GN=FSW04_11005 PE=3 SV=1
MM1 pKa = 7.58 TSSQMPTSTTMPRR14 pKa = 11.84 GARR17 pKa = 11.84 RR18 pKa = 11.84 SRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 RR24 pKa = 11.84 SRR26 pKa = 11.84 AARR29 pKa = 11.84 RR30 pKa = 11.84 SPRR33 pKa = 11.84 RR34 pKa = 11.84 CRR36 pKa = 11.84 TAGPSRR42 pKa = 11.84 ARR44 pKa = 11.84 RR45 pKa = 11.84 RR46 pKa = 11.84 GRR48 pKa = 11.84 RR49 pKa = 11.84 PPGPGARR56 pKa = 11.84 RR57 pKa = 11.84 GRR59 pKa = 11.84 GARR62 pKa = 11.84 APARR66 pKa = 11.84 ARR68 pKa = 11.84 ARR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 PRR74 pKa = 11.84 AVARR78 pKa = 11.84 PAPRR82 pKa = 11.84 RR83 pKa = 11.84 GRR85 pKa = 3.51
Molecular weight: 9.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.433
IPC_protein 13.042
Toseland 13.203
ProMoST 13.7
Dawson 13.203
Bjellqvist 13.203
Wikipedia 13.685
Rodwell 12.705
Grimsley 13.247
Solomon 13.7
Lehninger 13.598
Nozaki 13.203
DTASelect 13.203
Thurlkill 13.203
EMBOSS 13.7
Sillero 13.203
Patrickios 12.413
IPC_peptide 13.7
IPC2_peptide 12.691
IPC2.peptide.svr19 9.339
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4785
0
4785
1506352
29
2064
314.8
33.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.912 ± 0.067
0.858 ± 0.01
6.042 ± 0.028
5.461 ± 0.04
2.741 ± 0.021
9.729 ± 0.031
2.362 ± 0.017
3.56 ± 0.024
1.572 ± 0.024
10.303 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.765 ± 0.015
1.48 ± 0.017
6.043 ± 0.032
2.57 ± 0.019
8.882 ± 0.043
4.515 ± 0.03
5.423 ± 0.034
8.674 ± 0.033
1.337 ± 0.015
1.773 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here