Gordonia phage OhMyWard

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Deejayvirinae; Kenoshavirus; Gordonia virus OhMyWard

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8DA32|A0A5P8DA32_9CAUD Uncharacterized protein OS=Gordonia phage OhMyWard OX=2652414 GN=78 PE=4 SV=1
MM1 pKa = 7.54NCPKK5 pKa = 9.58CTAGPFEE12 pKa = 4.57NEE14 pKa = 4.16SLLIAHH20 pKa = 7.13WNDD23 pKa = 2.76VCTGSAEE30 pKa = 4.14GLQKK34 pKa = 10.78RR35 pKa = 11.84IDD37 pKa = 4.13DD38 pKa = 3.94FEE40 pKa = 5.14AAYY43 pKa = 11.01VEE45 pKa = 5.63FRR47 pKa = 11.84DD48 pKa = 3.76QQLDD52 pKa = 3.42AGEE55 pKa = 4.85HH56 pKa = 4.93YY57 pKa = 10.93VEE59 pKa = 4.1CTLYY63 pKa = 11.0NEE65 pKa = 4.79PEE67 pKa = 4.21SAARR71 pKa = 11.84PCSCGAIKK79 pKa = 10.24VVKK82 pKa = 10.12DD83 pKa = 3.14IPTTKK88 pKa = 10.22EE89 pKa = 3.42

Molecular weight: 9.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8D7B0|A0A5P8D7B0_9CAUD Holin OS=Gordonia phage OhMyWard OX=2652414 GN=14 PE=4 SV=1
MM1 pKa = 7.79PSIQLTHH8 pKa = 6.83KK9 pKa = 10.66GSFSNAQSRR18 pKa = 11.84LGRR21 pKa = 11.84MSKK24 pKa = 9.14GTIQDD29 pKa = 3.54ALSKK33 pKa = 10.5YY34 pKa = 9.34GALGVHH40 pKa = 6.0VLSNATPMDD49 pKa = 3.72SGEE52 pKa = 4.43SRR54 pKa = 11.84NAWTSKK60 pKa = 6.83VTKK63 pKa = 9.31TGKK66 pKa = 8.79GWKK69 pKa = 9.32LAWHH73 pKa = 6.07NQNKK77 pKa = 8.97TIDD80 pKa = 3.92GQPVVILLQFGHH92 pKa = 6.04GTGTGGYY99 pKa = 8.74VAGRR103 pKa = 11.84DD104 pKa = 4.24FINPAIKK111 pKa = 10.0PVFDD115 pKa = 5.28LIIAEE120 pKa = 4.28VRR122 pKa = 11.84RR123 pKa = 11.84KK124 pKa = 8.71VARR127 pKa = 3.73

Molecular weight: 13.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

19052

36

1700

221.5

24.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.797 ± 0.488

0.577 ± 0.1

6.257 ± 0.261

6.881 ± 0.29

3.532 ± 0.227

7.8 ± 0.442

1.989 ± 0.192

5.931 ± 0.237

6.02 ± 0.381

8.052 ± 0.346

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.892 ± 0.153

4.356 ± 0.168

4.267 ± 0.258

3.511 ± 0.139

5.013 ± 0.301

6.204 ± 0.226

6.362 ± 0.271

7.033 ± 0.228

1.286 ± 0.117

3.239 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski