Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Buchnera; Buchnera aphidicola; Buchnera aphidicola (Acyrthosiphon pisum)

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 572 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P57122|ATPA_BUCAI ATP synthase subunit alpha OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=atpA PE=3 SV=1
MM1 pKa = 7.65EE2 pKa = 5.25NLNVDD7 pKa = 3.63MLYY10 pKa = 10.06IAVAIMVGLASIGAAIGIGILGGKK34 pKa = 8.69FLEE37 pKa = 5.36GAARR41 pKa = 11.84QPDD44 pKa = 5.02LVPLLRR50 pKa = 11.84TQFFVVMGLVDD61 pKa = 6.56AIPMIAVGLGLYY73 pKa = 9.31MLFAISS79 pKa = 3.34

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P57132|MNME_BUCAI tRNA modification GTPase MnmE OS=Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) OX=107806 GN=mnmE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.53RR3 pKa = 11.84TFQPSILKK11 pKa = 10.25RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.1GFRR19 pKa = 11.84IRR21 pKa = 11.84MATKK25 pKa = 8.42NGRR28 pKa = 11.84YY29 pKa = 8.68ILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.5LRR39 pKa = 11.84TRR41 pKa = 11.84LTVSSKK47 pKa = 11.13

Molecular weight: 5.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

572

0

572

188274

38

1407

329.2

37.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.471 ± 0.083

1.215 ± 0.035

4.325 ± 0.061

5.512 ± 0.093

5.047 ± 0.096

5.423 ± 0.094

2.114 ± 0.033

11.544 ± 0.112

9.881 ± 0.138

9.907 ± 0.1

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.17 ± 0.042

7.174 ± 0.105

3.007 ± 0.046

3.202 ± 0.044

3.798 ± 0.079

7.271 ± 0.066

4.569 ± 0.057

4.852 ± 0.074

0.907 ± 0.032

3.611 ± 0.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski