Anaerocolumna aminovalerica
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4029 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I5GSW3|A0A1I5GSW3_9FIRM Lysophospholipase L1 OS=Anaerocolumna aminovalerica OX=1527 GN=SAMN04489757_12227 PE=4 SV=1
MM1 pKa = 7.95 DD2 pKa = 5.47 PSGTPTPAMPPLYY15 pKa = 9.86 PGFLINYY22 pKa = 7.44 FAYY25 pKa = 10.56 SQDD28 pKa = 3.15 GNDD31 pKa = 3.86 YY32 pKa = 10.75 FDD34 pKa = 5.64 SLDD37 pKa = 4.05 SDD39 pKa = 3.74 TKK41 pKa = 11.16 DD42 pKa = 3.4 YY43 pKa = 10.62 VAKK46 pKa = 10.57 HH47 pKa = 5.8 VDD49 pKa = 3.89 DD50 pKa = 5.08 LSSINDD56 pKa = 2.89 IKK58 pKa = 11.47 ACINEE63 pKa = 4.14 LNGDD67 pKa = 3.83 KK68 pKa = 11.01
Molecular weight: 7.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.808
IPC_protein 3.783
Toseland 3.541
ProMoST 3.999
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.821
Rodwell 3.605
Grimsley 3.465
Solomon 3.783
Lehninger 3.745
Nozaki 3.948
DTASelect 4.266
Thurlkill 3.643
EMBOSS 3.821
Sillero 3.91
Patrickios 0.782
IPC_peptide 3.783
IPC2_peptide 3.872
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A1I5HWN4|A0A1I5HWN4_9FIRM Uncharacterized protein YigZ family OS=Anaerocolumna aminovalerica OX=1527 GN=SAMN04489757_13524 PE=3 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 8.69 KK9 pKa = 7.75 RR10 pKa = 11.84 SRR12 pKa = 11.84 SRR14 pKa = 11.84 VHH16 pKa = 6.58 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTSNGRR28 pKa = 11.84 KK29 pKa = 8.69 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.26 GRR39 pKa = 11.84 HH40 pKa = 5.5 KK41 pKa = 10.9 LSAA44 pKa = 3.8
Molecular weight: 5.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.508
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.91
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.647
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.369
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.132
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4029
0
4029
1286805
30
2040
319.4
35.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.306 ± 0.041
1.179 ± 0.013
5.443 ± 0.027
7.407 ± 0.041
4.28 ± 0.023
6.682 ± 0.036
1.634 ± 0.014
8.978 ± 0.04
7.746 ± 0.035
9.097 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.828 ± 0.018
5.477 ± 0.031
3.146 ± 0.022
2.964 ± 0.019
3.625 ± 0.024
6.162 ± 0.029
5.451 ± 0.029
6.451 ± 0.029
0.81 ± 0.01
4.335 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here