Mycobacterium phage FlagStaff

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6WE06|A0A0F6WE06_9CAUD Capsid maturation protease OS=Mycobacterium phage FlagStaff OX=1647304 GN=4 PE=4 SV=1
MM1 pKa = 7.25AHH3 pKa = 6.14SPAMRR8 pKa = 11.84AALDD12 pKa = 3.9ALDD15 pKa = 3.75VAIVDD20 pKa = 3.5VFEE23 pKa = 5.92AIRR26 pKa = 11.84SDD28 pKa = 5.08DD29 pKa = 4.31PDD31 pKa = 4.17DD32 pKa = 4.29TPPDD36 pKa = 4.73AIAVDD41 pKa = 4.07AVLLIGVQHH50 pKa = 7.04IDD52 pKa = 4.56DD53 pKa = 4.82DD54 pKa = 4.36DD55 pKa = 4.95ARR57 pKa = 11.84IGYY60 pKa = 7.39TEE62 pKa = 3.84VVPRR66 pKa = 11.84YY67 pKa = 8.29GAQPAYY73 pKa = 8.64VTRR76 pKa = 11.84GLIGDD81 pKa = 4.4ALHH84 pKa = 7.31LIDD87 pKa = 4.99QVADD91 pKa = 4.73AGDD94 pKa = 3.57QDD96 pKa = 3.74QGGG99 pKa = 3.45

Molecular weight: 10.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6SJM7|A0A0F6SJM7_9CAUD Uncharacterized protein OS=Mycobacterium phage FlagStaff OX=1647304 GN=50 PE=4 SV=1
MM1 pKa = 7.35MKK3 pKa = 9.96TGGNGIGIGTVLFLVFLVLKK23 pKa = 8.21LTHH26 pKa = 6.29VIAWSWLWVTAPLWIPLAVLLVALLVALLLGGRR59 pKa = 11.84PRR61 pKa = 11.84SKK63 pKa = 10.62RR64 pKa = 3.41

Molecular weight: 6.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

14634

43

1344

225.1

24.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.551 ± 0.694

1.032 ± 0.202

7.524 ± 0.402

5.337 ± 0.407

2.911 ± 0.21

9.273 ± 0.727

1.961 ± 0.217

4.175 ± 0.204

3.403 ± 0.269

7.394 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.057 ± 0.115

3.232 ± 0.172

6.164 ± 0.422

3.622 ± 0.17

6.765 ± 0.528

4.524 ± 0.265

6.287 ± 0.283

6.717 ± 0.207

2.057 ± 0.147

2.016 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski