Amino acid dipepetide frequency for Bacillus phage BCP12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.904AlaAla: 4.904 ± 0.466
0.294AlaCys: 0.294 ± 0.092
3.776AlaAsp: 3.776 ± 0.311
5.051AlaGlu: 5.051 ± 0.488
2.378AlaPhe: 2.378 ± 0.234
3.629AlaGly: 3.629 ± 0.386
1.177AlaHis: 1.177 ± 0.174
4.561AlaIle: 4.561 ± 0.401
5.272AlaLys: 5.272 ± 0.402
5.37AlaLeu: 5.37 ± 0.363
1.912AlaMet: 1.912 ± 0.2
3.089AlaAsn: 3.089 ± 0.422
2.207AlaPro: 2.207 ± 0.273
2.844AlaGln: 2.844 ± 0.345
3.089AlaArg: 3.089 ± 0.333
3.335AlaSer: 3.335 ± 0.311
4.438AlaThr: 4.438 ± 0.4
4.07AlaVal: 4.07 ± 0.285
1.128AlaTrp: 1.128 ± 0.146
2.746AlaTyr: 2.746 ± 0.223
0.0AlaXaa: 0.0 ± 0.0
Cys
0.294CysAla: 0.294 ± 0.071
0.098CysCys: 0.098 ± 0.054
0.343CysAsp: 0.343 ± 0.094
0.245CysGlu: 0.245 ± 0.075
0.27CysPhe: 0.27 ± 0.081
0.662CysGly: 0.662 ± 0.135
0.245CysHis: 0.245 ± 0.08
0.466CysIle: 0.466 ± 0.116
0.711CysLys: 0.711 ± 0.163
0.515CysLeu: 0.515 ± 0.114
0.319CysMet: 0.319 ± 0.073
0.662CysAsn: 0.662 ± 0.135
0.466CysPro: 0.466 ± 0.096
0.074CysGln: 0.074 ± 0.044
0.27CysArg: 0.27 ± 0.087
0.564CysSer: 0.564 ± 0.121
0.49CysThr: 0.49 ± 0.11
0.466CysVal: 0.466 ± 0.106
0.025CysTrp: 0.025 ± 0.023
0.368CysTyr: 0.368 ± 0.104
0.0CysXaa: 0.0 ± 0.0
Asp
3.849AspAla: 3.849 ± 0.26
0.417AspCys: 0.417 ± 0.103
2.771AspAsp: 2.771 ± 0.294
4.536AspGlu: 4.536 ± 0.353
2.746AspPhe: 2.746 ± 0.258
3.825AspGly: 3.825 ± 0.318
1.03AspHis: 1.03 ± 0.176
4.659AspIle: 4.659 ± 0.342
4.953AspLys: 4.953 ± 0.358
4.683AspLeu: 4.683 ± 0.352
1.667AspMet: 1.667 ± 0.225
3.016AspAsn: 3.016 ± 0.37
1.594AspPro: 1.594 ± 0.187
1.152AspGln: 1.152 ± 0.159
2.697AspArg: 2.697 ± 0.246
3.04AspSer: 3.04 ± 0.285
3.016AspThr: 3.016 ± 0.272
4.34AspVal: 4.34 ± 0.303
0.711AspTrp: 0.711 ± 0.137
2.991AspTyr: 2.991 ± 0.289
0.0AspXaa: 0.0 ± 0.0
Glu
4.413GluAla: 4.413 ± 0.396
0.687GluCys: 0.687 ± 0.13
4.634GluAsp: 4.634 ± 0.405
8.484GluGlu: 8.484 ± 0.87
3.114GluPhe: 3.114 ± 0.312
4.757GluGly: 4.757 ± 0.425
1.594GluHis: 1.594 ± 0.214
4.83GluIle: 4.83 ± 0.373
6.694GluLys: 6.694 ± 0.468
7.429GluLeu: 7.429 ± 0.413
2.158GluMet: 2.158 ± 0.256
3.433GluAsn: 3.433 ± 0.338
2.354GluPro: 2.354 ± 0.514
3.016GluGln: 3.016 ± 0.272
3.653GluArg: 3.653 ± 0.379
3.212GluSer: 3.212 ± 0.248
3.898GluThr: 3.898 ± 0.289
5.615GluVal: 5.615 ± 0.517
1.03GluTrp: 1.03 ± 0.15
3.359GluTyr: 3.359 ± 0.346
0.0GluXaa: 0.0 ± 0.0
Phe
2.55PheAla: 2.55 ± 0.246
0.466PheCys: 0.466 ± 0.102
2.844PheAsp: 2.844 ± 0.266
2.378PheGlu: 2.378 ± 0.285
1.398PhePhe: 1.398 ± 0.227
2.403PheGly: 2.403 ± 0.278
0.932PheHis: 0.932 ± 0.175
2.207PheIle: 2.207 ± 0.252
3.163PheLys: 3.163 ± 0.278
3.408PheLeu: 3.408 ± 0.296
1.25PheMet: 1.25 ± 0.187
2.109PheAsn: 2.109 ± 0.24
1.103PhePro: 1.103 ± 0.15
1.398PheGln: 1.398 ± 0.2
1.79PheArg: 1.79 ± 0.194
2.918PheSer: 2.918 ± 0.269
2.869PheThr: 2.869 ± 0.284
2.893PheVal: 2.893 ± 0.273
0.27PheTrp: 0.27 ± 0.089
1.765PheTyr: 1.765 ± 0.206
0.0PheXaa: 0.0 ± 0.0
Gly
3.923GlyAla: 3.923 ± 0.337
0.466GlyCys: 0.466 ± 0.126
3.286GlyAsp: 3.286 ± 0.313
4.315GlyGlu: 4.315 ± 0.344
2.746GlyPhe: 2.746 ± 0.281
3.849GlyGly: 3.849 ± 0.434
1.324GlyHis: 1.324 ± 0.195
3.751GlyIle: 3.751 ± 0.308
5.051GlyLys: 5.051 ± 0.361
4.806GlyLeu: 4.806 ± 0.4
1.839GlyMet: 1.839 ± 0.203
3.482GlyAsn: 3.482 ± 0.455
0.0GlyPro: 0.0 ± 0.0
2.256GlyGln: 2.256 ± 0.227
2.599GlyArg: 2.599 ± 0.246
4.511GlySer: 4.511 ± 0.554
5.149GlyThr: 5.149 ± 0.436
4.585GlyVal: 4.585 ± 0.363
0.809GlyTrp: 0.809 ± 0.157
2.918GlyTyr: 2.918 ± 0.271
0.0GlyXaa: 0.0 ± 0.0
His
0.981HisAla: 0.981 ± 0.176
0.172HisCys: 0.172 ± 0.06
1.201HisAsp: 1.201 ± 0.172
1.201HisGlu: 1.201 ± 0.192
0.76HisPhe: 0.76 ± 0.139
1.103HisGly: 1.103 ± 0.199
0.392HisHis: 0.392 ± 0.096
1.103HisIle: 1.103 ± 0.178
1.324HisLys: 1.324 ± 0.186
1.79HisLeu: 1.79 ± 0.248
0.441HisMet: 0.441 ± 0.089
1.054HisAsn: 1.054 ± 0.153
0.956HisPro: 0.956 ± 0.163
0.392HisGln: 0.392 ± 0.081
1.152HisArg: 1.152 ± 0.205
1.079HisSer: 1.079 ± 0.167
1.152HisThr: 1.152 ± 0.144
1.569HisVal: 1.569 ± 0.178
0.27HisTrp: 0.27 ± 0.081
1.005HisTyr: 1.005 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
3.972IleAla: 3.972 ± 0.312
0.564IleCys: 0.564 ± 0.113
4.193IleAsp: 4.193 ± 0.361
5.223IleGlu: 5.223 ± 0.384
2.305IlePhe: 2.305 ± 0.235
4.095IleGly: 4.095 ± 0.349
1.103IleHis: 1.103 ± 0.166
4.07IleIle: 4.07 ± 0.354
4.953IleLys: 4.953 ± 0.325
4.977IleLeu: 4.977 ± 0.415
1.667IleMet: 1.667 ± 0.235
3.678IleAsn: 3.678 ± 0.294
2.991IlePro: 2.991 ± 0.267
2.501IleGln: 2.501 ± 0.205
2.771IleArg: 2.771 ± 0.267
4.438IleSer: 4.438 ± 0.293
4.536IleThr: 4.536 ± 0.333
4.389IleVal: 4.389 ± 0.337
0.515IleTrp: 0.515 ± 0.116
2.476IleTyr: 2.476 ± 0.217
0.0IleXaa: 0.0 ± 0.0
Lys
5.173LysAla: 5.173 ± 0.307
0.637LysCys: 0.637 ± 0.125
4.291LysAsp: 4.291 ± 0.373
8.067LysGlu: 8.067 ± 0.52
2.967LysPhe: 2.967 ± 0.252
4.953LysGly: 4.953 ± 0.429
1.692LysHis: 1.692 ± 0.232
4.193LysIle: 4.193 ± 0.28
7.356LysLys: 7.356 ± 0.53
6.645LysLeu: 6.645 ± 0.384
2.795LysMet: 2.795 ± 0.242
3.604LysAsn: 3.604 ± 0.319
2.991LysPro: 2.991 ± 0.298
3.849LysGln: 3.849 ± 0.302
3.849LysArg: 3.849 ± 0.354
3.555LysSer: 3.555 ± 0.394
4.046LysThr: 4.046 ± 0.287
5.934LysVal: 5.934 ± 0.404
0.883LysTrp: 0.883 ± 0.143
3.31LysTyr: 3.31 ± 0.264
0.0LysXaa: 0.0 ± 0.0
Leu
5.983LeuAla: 5.983 ± 0.367
0.515LeuCys: 0.515 ± 0.109
5.247LeuAsp: 5.247 ± 0.356
6.89LeuGlu: 6.89 ± 0.486
3.236LeuPhe: 3.236 ± 0.268
4.708LeuGly: 4.708 ± 0.324
1.471LeuHis: 1.471 ± 0.196
5.002LeuIle: 5.002 ± 0.305
6.816LeuLys: 6.816 ± 0.46
5.541LeuLeu: 5.541 ± 0.45
2.084LeuMet: 2.084 ± 0.29
4.046LeuAsn: 4.046 ± 0.341
2.918LeuPro: 2.918 ± 0.279
2.918LeuGln: 2.918 ± 0.263
3.923LeuArg: 3.923 ± 0.282
4.561LeuSer: 4.561 ± 0.319
5.321LeuThr: 5.321 ± 0.362
5.517LeuVal: 5.517 ± 0.393
0.809LeuTrp: 0.809 ± 0.145
3.089LeuTyr: 3.089 ± 0.29
0.0LeuXaa: 0.0 ± 0.0
Met
2.256MetAla: 2.256 ± 0.229
0.172MetCys: 0.172 ± 0.062
1.52MetAsp: 1.52 ± 0.25
2.329MetGlu: 2.329 ± 0.258
1.128MetPhe: 1.128 ± 0.149
1.03MetGly: 1.03 ± 0.159
0.539MetHis: 0.539 ± 0.124
2.011MetIle: 2.011 ± 0.242
2.771MetLys: 2.771 ± 0.252
2.231MetLeu: 2.231 ± 0.307
0.588MetMet: 0.588 ± 0.107
1.741MetAsn: 1.741 ± 0.207
0.687MetPro: 0.687 ± 0.108
1.079MetGln: 1.079 ± 0.169
1.52MetArg: 1.52 ± 0.163
1.545MetSer: 1.545 ± 0.206
1.765MetThr: 1.765 ± 0.188
1.618MetVal: 1.618 ± 0.177
0.221MetTrp: 0.221 ± 0.075
1.25MetTyr: 1.25 ± 0.171
0.0MetXaa: 0.0 ± 0.0
Asn
3.335AsnAla: 3.335 ± 0.29
0.515AsnCys: 0.515 ± 0.131
2.084AsnAsp: 2.084 ± 0.195
3.874AsnGlu: 3.874 ± 0.314
1.618AsnPhe: 1.618 ± 0.226
4.511AsnGly: 4.511 ± 0.443
1.177AsnHis: 1.177 ± 0.164
3.825AsnIle: 3.825 ± 0.318
4.119AsnLys: 4.119 ± 0.305
3.776AsnLeu: 3.776 ± 0.305
1.986AsnMet: 1.986 ± 0.217
3.212AsnAsn: 3.212 ± 0.335
2.746AsnPro: 2.746 ± 0.329
1.986AsnGln: 1.986 ± 0.204
2.55AsnArg: 2.55 ± 0.258
2.501AsnSer: 2.501 ± 0.297
3.187AsnThr: 3.187 ± 0.338
3.335AsnVal: 3.335 ± 0.324
0.662AsnTrp: 0.662 ± 0.122
2.256AsnTyr: 2.256 ± 0.275
0.0AsnXaa: 0.0 ± 0.0
Pro
2.133ProAla: 2.133 ± 0.244
0.245ProCys: 0.245 ± 0.101
1.692ProAsp: 1.692 ± 0.19
2.354ProGlu: 2.354 ± 0.281
1.201ProPhe: 1.201 ± 0.158
1.741ProGly: 1.741 ± 0.258
0.883ProHis: 0.883 ± 0.151
2.305ProIle: 2.305 ± 0.243
2.697ProLys: 2.697 ± 0.228
2.427ProLeu: 2.427 ± 0.231
0.834ProMet: 0.834 ± 0.158
2.28ProAsn: 2.28 ± 0.288
1.128ProPro: 1.128 ± 0.171
1.447ProGln: 1.447 ± 0.217
1.299ProArg: 1.299 ± 0.131
1.692ProSer: 1.692 ± 0.235
2.722ProThr: 2.722 ± 0.307
3.016ProVal: 3.016 ± 0.361
0.27ProTrp: 0.27 ± 0.086
1.594ProTyr: 1.594 ± 0.208
0.0ProXaa: 0.0 ± 0.0
Gln
3.04GlnAla: 3.04 ± 0.274
0.245GlnCys: 0.245 ± 0.083
1.863GlnAsp: 1.863 ± 0.23
3.678GlnGlu: 3.678 ± 0.327
1.324GlnPhe: 1.324 ± 0.159
2.28GlnGly: 2.28 ± 0.211
0.588GlnHis: 0.588 ± 0.117
2.354GlnIle: 2.354 ± 0.223
2.624GlnLys: 2.624 ± 0.214
3.138GlnLeu: 3.138 ± 0.264
1.373GlnMet: 1.373 ± 0.204
1.545GlnAsn: 1.545 ± 0.169
1.324GlnPro: 1.324 ± 0.26
2.207GlnGln: 2.207 ± 0.268
1.398GlnArg: 1.398 ± 0.188
2.035GlnSer: 2.035 ± 0.196
2.109GlnThr: 2.109 ± 0.21
2.746GlnVal: 2.746 ± 0.252
0.466GlnTrp: 0.466 ± 0.103
1.25GlnTyr: 1.25 ± 0.161
0.0GlnXaa: 0.0 ± 0.0
Arg
2.55ArgAla: 2.55 ± 0.354
0.294ArgCys: 0.294 ± 0.099
2.378ArgAsp: 2.378 ± 0.208
3.089ArgGlu: 3.089 ± 0.309
2.06ArgPhe: 2.06 ± 0.239
2.648ArgGly: 2.648 ± 0.275
0.588ArgHis: 0.588 ± 0.138
2.869ArgIle: 2.869 ± 0.245
4.07ArgLys: 4.07 ± 0.327
4.046ArgLeu: 4.046 ± 0.304
1.814ArgMet: 1.814 ± 0.218
2.746ArgAsn: 2.746 ± 0.274
1.324ArgPro: 1.324 ± 0.178
1.643ArgGln: 1.643 ± 0.207
2.182ArgArg: 2.182 ± 0.256
2.476ArgSer: 2.476 ± 0.259
2.403ArgThr: 2.403 ± 0.24
3.089ArgVal: 3.089 ± 0.303
0.515ArgTrp: 0.515 ± 0.113
2.256ArgTyr: 2.256 ± 0.238
0.0ArgXaa: 0.0 ± 0.0
Ser
3.433SerAla: 3.433 ± 0.301
0.368SerCys: 0.368 ± 0.096
3.727SerAsp: 3.727 ± 0.269
3.163SerGlu: 3.163 ± 0.332
2.697SerPhe: 2.697 ± 0.288
4.266SerGly: 4.266 ± 0.485
0.932SerHis: 0.932 ± 0.139
3.948SerIle: 3.948 ± 0.276
4.389SerLys: 4.389 ± 0.352
4.683SerLeu: 4.683 ± 0.334
1.275SerMet: 1.275 ± 0.167
2.722SerAsn: 2.722 ± 0.332
1.79SerPro: 1.79 ± 0.21
1.741SerGln: 1.741 ± 0.231
2.231SerArg: 2.231 ± 0.239
3.653SerSer: 3.653 ± 0.759
3.948SerThr: 3.948 ± 0.41
3.849SerVal: 3.849 ± 0.322
0.858SerTrp: 0.858 ± 0.166
2.378SerTyr: 2.378 ± 0.232
0.0SerXaa: 0.0 ± 0.0
Thr
3.825ThrAla: 3.825 ± 0.547
0.49ThrCys: 0.49 ± 0.101
3.555ThrAsp: 3.555 ± 0.286
4.708ThrGlu: 4.708 ± 0.4
3.065ThrPhe: 3.065 ± 0.283
4.61ThrGly: 4.61 ± 0.416
1.054ThrHis: 1.054 ± 0.153
4.708ThrIle: 4.708 ± 0.365
4.413ThrLys: 4.413 ± 0.312
5.124ThrLeu: 5.124 ± 0.329
1.054ThrMet: 1.054 ± 0.149
3.089ThrAsn: 3.089 ± 0.315
2.942ThrPro: 2.942 ± 0.3
1.79ThrGln: 1.79 ± 0.162
2.476ThrArg: 2.476 ± 0.293
3.482ThrSer: 3.482 ± 0.272
3.678ThrThr: 3.678 ± 0.375
5.419ThrVal: 5.419 ± 0.449
0.662ThrTrp: 0.662 ± 0.133
3.089ThrTyr: 3.089 ± 0.336
0.0ThrXaa: 0.0 ± 0.0
Val
5.002ValAla: 5.002 ± 0.325
0.417ValCys: 0.417 ± 0.105
4.34ValAsp: 4.34 ± 0.323
5.345ValGlu: 5.345 ± 0.451
3.089ValPhe: 3.089 ± 0.333
3.653ValGly: 3.653 ± 0.3
1.226ValHis: 1.226 ± 0.217
4.904ValIle: 4.904 ± 0.321
5.615ValLys: 5.615 ± 0.426
5.075ValLeu: 5.075 ± 0.457
1.765ValMet: 1.765 ± 0.226
3.972ValAsn: 3.972 ± 0.339
3.089ValPro: 3.089 ± 0.318
3.065ValGln: 3.065 ± 0.261
3.261ValArg: 3.261 ± 0.275
4.168ValSer: 4.168 ± 0.344
4.659ValThr: 4.659 ± 0.425
4.904ValVal: 4.904 ± 0.393
0.662ValTrp: 0.662 ± 0.132
3.114ValTyr: 3.114 ± 0.275
0.0ValXaa: 0.0 ± 0.0
Trp
0.834TrpAla: 0.834 ± 0.165
0.221TrpCys: 0.221 ± 0.064
0.932TrpAsp: 0.932 ± 0.148
0.858TrpGlu: 0.858 ± 0.134
0.515TrpPhe: 0.515 ± 0.129
0.441TrpGly: 0.441 ± 0.109
0.196TrpHis: 0.196 ± 0.067
0.662TrpIle: 0.662 ± 0.118
0.809TrpLys: 0.809 ± 0.146
1.005TrpLeu: 1.005 ± 0.162
0.123TrpMet: 0.123 ± 0.053
0.834TrpAsn: 0.834 ± 0.157
0.0TrpPro: 0.0 ± 0.0
0.466TrpGln: 0.466 ± 0.119
0.27TrpArg: 0.27 ± 0.099
0.809TrpSer: 0.809 ± 0.145
0.834TrpThr: 0.834 ± 0.153
0.809TrpVal: 0.809 ± 0.151
0.147TrpTrp: 0.147 ± 0.054
0.662TrpTyr: 0.662 ± 0.13
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.82TyrAla: 2.82 ± 0.25
0.27TyrCys: 0.27 ± 0.086
3.04TyrAsp: 3.04 ± 0.269
2.574TyrGlu: 2.574 ± 0.211
1.496TyrPhe: 1.496 ± 0.197
2.501TyrGly: 2.501 ± 0.303
0.956TyrHis: 0.956 ± 0.182
2.991TyrIle: 2.991 ± 0.269
2.991TyrLys: 2.991 ± 0.239
3.923TyrLeu: 3.923 ± 0.314
1.03TyrMet: 1.03 ± 0.141
2.942TyrAsn: 2.942 ± 0.253
1.349TyrPro: 1.349 ± 0.185
1.765TyrGln: 1.765 ± 0.184
2.06TyrArg: 2.06 ± 0.242
2.476TyrSer: 2.476 ± 0.27
3.016TyrThr: 3.016 ± 0.26
3.163TyrVal: 3.163 ± 0.261
0.515TyrTrp: 0.515 ± 0.098
1.692TyrTyr: 1.692 ± 0.232
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 229 proteins (40786 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski