Roseburia phage Shimadzu

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A509ENL9|A0A509ENL9_9CAUD Portal OS=Roseburia phage Shimadzu OX=2570913 GN=orf04 PE=4 SV=1
MM1 pKa = 7.22YY2 pKa = 10.35NDD4 pKa = 3.89EE5 pKa = 4.74RR6 pKa = 11.84LAHH9 pKa = 6.44YY10 pKa = 9.89IIEE13 pKa = 4.31YY14 pKa = 9.95NPDD17 pKa = 3.4YY18 pKa = 11.33ADD20 pKa = 3.51VIVFEE25 pKa = 5.22GGVQLKK31 pKa = 10.69LPVVEE36 pKa = 4.36EE37 pKa = 4.64AEE39 pKa = 4.32TSEE42 pKa = 4.53TVPPWRR48 pKa = 11.84ATAA51 pKa = 3.78

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A509ER20|A0A509ER20_9CAUD Uncharacterized protein OS=Roseburia phage Shimadzu OX=2570913 GN=orf25 PE=4 SV=1
MM1 pKa = 7.45LTRR4 pKa = 11.84ALNDD8 pKa = 3.52LKK10 pKa = 11.15NPKK13 pKa = 9.92SKK15 pKa = 9.44TGSLQIIATFTGTTGSMGFVTGQRR39 pKa = 11.84YY40 pKa = 8.3EE41 pKa = 4.19LIVRR45 pKa = 11.84YY46 pKa = 9.35IRR48 pKa = 11.84SRR50 pKa = 11.84GRR52 pKa = 11.84FEE54 pKa = 4.41VKK56 pKa = 9.57TRR58 pKa = 11.84DD59 pKa = 3.53GQLFCPYY66 pKa = 10.42QSTEE70 pKa = 3.73AFAKK74 pKa = 9.8NWSASAIQKK83 pKa = 9.46GAA85 pKa = 3.22

Molecular weight: 9.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12942

51

756

219.4

24.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.736 ± 0.431

1.19 ± 0.125

5.795 ± 0.252

7.781 ± 0.365

3.199 ± 0.148

7.016 ± 0.286

1.429 ± 0.123

6.351 ± 0.269

6.63 ± 0.316

7.34 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.148

4.142 ± 0.214

4.319 ± 0.207

4.01 ± 0.26

4.968 ± 0.386

5.764 ± 0.344

6.9 ± 0.273

5.803 ± 0.341

1.375 ± 0.137

3.763 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski