Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma genitalium

Average proteome isoelectric point is 7.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 483 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q49416|Y315_MYCGE Uncharacterized protein MG315 OS=Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) OX=243273 GN=MG315 PE=4 SV=1
MM1 pKa = 7.8AKK3 pKa = 10.05NKK5 pKa = 10.1QSVFEE10 pKa = 4.33EE11 pKa = 4.06KK12 pKa = 10.62NYY14 pKa = 9.16TQTEE18 pKa = 4.21PEE20 pKa = 4.46NIFGDD25 pKa = 4.13LYY27 pKa = 10.96DD28 pKa = 4.14GKK30 pKa = 9.92STVEE34 pKa = 3.81EE35 pKa = 4.29DD36 pKa = 3.62PNIKK40 pKa = 9.73VAYY43 pKa = 9.7DD44 pKa = 3.48ADD46 pKa = 3.92GNGYY50 pKa = 10.23YY51 pKa = 9.77IAFNKK56 pKa = 7.51EE57 pKa = 3.54TGVYY61 pKa = 8.99YY62 pKa = 10.46DD63 pKa = 4.21PYY65 pKa = 11.39GDD67 pKa = 3.73TEE69 pKa = 4.27YY70 pKa = 10.98DD71 pKa = 2.89ISQLFDD77 pKa = 3.55EE78 pKa = 5.14NGNPFVFDD86 pKa = 3.98EE87 pKa = 4.32KK88 pKa = 11.11QEE90 pKa = 4.01EE91 pKa = 4.06NDD93 pKa = 3.24YY94 pKa = 11.22LKK96 pKa = 11.13YY97 pKa = 10.76VGNPDD102 pKa = 3.29YY103 pKa = 11.33GSYY106 pKa = 10.8DD107 pKa = 3.73EE108 pKa = 4.59NGEE111 pKa = 4.44WVWSGYY117 pKa = 9.4FEE119 pKa = 4.58NDD121 pKa = 2.39QWISTKK127 pKa = 10.17EE128 pKa = 3.94SQPTDD133 pKa = 3.12EE134 pKa = 5.56NYY136 pKa = 10.95GFDD139 pKa = 3.5SDD141 pKa = 5.25LPPEE145 pKa = 4.45VKK147 pKa = 10.3QPEE150 pKa = 4.54SVEE153 pKa = 4.07DD154 pKa = 3.97NYY156 pKa = 11.61GFDD159 pKa = 3.62NDD161 pKa = 4.66LPPEE165 pKa = 4.32VKK167 pKa = 10.29QPEE170 pKa = 4.54SVEE173 pKa = 4.07DD174 pKa = 3.97NYY176 pKa = 11.61GFDD179 pKa = 3.62NDD181 pKa = 4.66LPPEE185 pKa = 4.32VKK187 pKa = 10.33QPEE190 pKa = 4.56SVVDD194 pKa = 3.83QPSSDD199 pKa = 4.5DD200 pKa = 3.74YY201 pKa = 11.12FAKK204 pKa = 10.55QPTDD208 pKa = 3.07EE209 pKa = 5.49NYY211 pKa = 10.95GFDD214 pKa = 3.6NDD216 pKa = 4.58LPPEE220 pKa = 4.32VKK222 pKa = 10.33QPEE225 pKa = 4.56SVVDD229 pKa = 3.81QPSSDD234 pKa = 3.55DD235 pKa = 3.91HH236 pKa = 6.48FAKK239 pKa = 10.46QPEE242 pKa = 4.67STTDD246 pKa = 3.06SYY248 pKa = 12.08SFDD251 pKa = 3.63SDD253 pKa = 4.32LPQPTLDD260 pKa = 3.76QPSLDD265 pKa = 4.04DD266 pKa = 3.59HH267 pKa = 6.0VQYY270 pKa = 11.32NFDD273 pKa = 3.2HH274 pKa = 7.15HH275 pKa = 7.07EE276 pKa = 3.93EE277 pKa = 4.08LKK279 pKa = 10.82PVAEE283 pKa = 4.32EE284 pKa = 3.74QNNYY288 pKa = 7.85QVGFDD293 pKa = 3.48QVQANLDD300 pKa = 3.89NNEE303 pKa = 4.37EE304 pKa = 4.04IQPTAEE310 pKa = 4.33KK311 pKa = 10.63KK312 pKa = 8.8VTTDD316 pKa = 4.07FEE318 pKa = 4.83SKK320 pKa = 9.39QAQVVDD326 pKa = 4.67SYY328 pKa = 11.47QLPIDD333 pKa = 4.43TDD335 pKa = 3.66QQDD338 pKa = 3.22QTTFSSSFEE347 pKa = 4.09TQPTVEE353 pKa = 5.2QFDD356 pKa = 3.93QVNSEE361 pKa = 4.27VNDD364 pKa = 3.4QFKK367 pKa = 11.16PEE369 pKa = 3.92ITKK372 pKa = 10.68EE373 pKa = 3.99PVLEE377 pKa = 4.19SSFNKK382 pKa = 9.67QDD384 pKa = 3.46VVEE387 pKa = 4.26TSDD390 pKa = 5.04LNSEE394 pKa = 4.35SNLYY398 pKa = 10.44SEE400 pKa = 4.7NNKK403 pKa = 10.25DD404 pKa = 3.22ATNNDD409 pKa = 3.48SLNSEE414 pKa = 5.14FIQLNSNSEE423 pKa = 4.05TASDD427 pKa = 3.72DD428 pKa = 3.2VHH430 pKa = 7.83YY431 pKa = 9.98EE432 pKa = 4.18SKK434 pKa = 10.7SEE436 pKa = 4.51PIHH439 pKa = 6.89DD440 pKa = 3.96YY441 pKa = 11.35KK442 pKa = 11.08FGSDD446 pKa = 3.38LSQSNSNNSLEE457 pKa = 4.13SEE459 pKa = 4.01PVKK462 pKa = 10.75FNSEE466 pKa = 4.23TAPDD470 pKa = 3.34AHH472 pKa = 6.94FEE474 pKa = 4.4SQSEE478 pKa = 4.18PVDD481 pKa = 3.38QVQYY485 pKa = 10.96DD486 pKa = 3.8IYY488 pKa = 11.12QNEE491 pKa = 4.23EE492 pKa = 4.14LKK494 pKa = 9.5PTLDD498 pKa = 3.66QPSSDD503 pKa = 4.97DD504 pKa = 3.73YY505 pKa = 11.12FAKK508 pKa = 10.55QPTDD512 pKa = 3.07EE513 pKa = 5.49NYY515 pKa = 10.95GFDD518 pKa = 3.6NDD520 pKa = 4.58LPPEE524 pKa = 4.32VKK526 pKa = 10.33QPEE529 pKa = 4.56SVVDD533 pKa = 3.81QPSSDD538 pKa = 3.55DD539 pKa = 3.91HH540 pKa = 6.48FAKK543 pKa = 10.46QPEE546 pKa = 4.67STTDD550 pKa = 3.06SYY552 pKa = 12.08SFDD555 pKa = 3.63SDD557 pKa = 4.32LPQPTLDD564 pKa = 3.76QPSLDD569 pKa = 4.04DD570 pKa = 3.59HH571 pKa = 6.0VQYY574 pKa = 11.32NFDD577 pKa = 3.2HH578 pKa = 7.15HH579 pKa = 7.07EE580 pKa = 3.93EE581 pKa = 4.08LKK583 pKa = 10.82PVAEE587 pKa = 4.32EE588 pKa = 3.74QNNYY592 pKa = 7.85QVGFDD597 pKa = 3.48QVQANLDD604 pKa = 3.89NNEE607 pKa = 4.37EE608 pKa = 4.04IQPTAEE614 pKa = 4.42KK615 pKa = 10.55EE616 pKa = 4.16VTTDD620 pKa = 4.16FEE622 pKa = 4.82SKK624 pKa = 9.39QAQVVDD630 pKa = 4.67SYY632 pKa = 11.47QLPIDD637 pKa = 4.43TDD639 pKa = 3.66QQDD642 pKa = 3.22QTTFSSSFEE651 pKa = 4.09TQPTVEE657 pKa = 5.2QFDD660 pKa = 3.93QVNSEE665 pKa = 4.27VNDD668 pKa = 3.4QFKK671 pKa = 11.16PEE673 pKa = 3.92ITKK676 pKa = 10.68EE677 pKa = 3.99PVLEE681 pKa = 4.19SSFNKK686 pKa = 9.67QDD688 pKa = 3.4VVEE691 pKa = 4.29TSNYY695 pKa = 8.6TNNLQKK701 pKa = 10.58FDD703 pKa = 3.65IQSDD707 pKa = 3.67NKK709 pKa = 9.66ITITTKK715 pKa = 10.32KK716 pKa = 9.98SSPQIPTTLPISFVSNRR733 pKa = 11.84IEE735 pKa = 4.09YY736 pKa = 10.5KK737 pKa = 10.26PVEE740 pKa = 4.31TLALDD745 pKa = 3.74NKK747 pKa = 10.22EE748 pKa = 4.17SQQEE752 pKa = 4.04QITINSITEE761 pKa = 3.93DD762 pKa = 3.6SKK764 pKa = 10.29TLAKK768 pKa = 9.54TLSVQLQQINSLNNQSIVTSEE789 pKa = 4.16SVRR792 pKa = 11.84LDD794 pKa = 3.46KK795 pKa = 11.16KK796 pKa = 10.91DD797 pKa = 3.64DD798 pKa = 3.5QLTINTVNSEE808 pKa = 4.0DD809 pKa = 3.73QQPKK813 pKa = 9.92IEE815 pKa = 4.33VFVKK819 pKa = 10.42AKK821 pKa = 10.5EE822 pKa = 3.97PVEE825 pKa = 4.03EE826 pKa = 5.14HH827 pKa = 7.21SITQNKK833 pKa = 8.69QSVEE837 pKa = 4.21DD838 pKa = 4.46KK839 pKa = 11.42SEE841 pKa = 4.0LDD843 pKa = 3.55NFNKK847 pKa = 10.34KK848 pKa = 10.01SDD850 pKa = 3.47LYY852 pKa = 11.22KK853 pKa = 10.38IISEE857 pKa = 4.37LKK859 pKa = 10.06RR860 pKa = 11.84GEE862 pKa = 4.32LNPTINFDD870 pKa = 6.18AIFQMNDD877 pKa = 2.64YY878 pKa = 10.68QMSVKK883 pKa = 10.34QSFIHH888 pKa = 7.09LNDD891 pKa = 3.29FVTNYY896 pKa = 10.1KK897 pKa = 9.89NQISEE902 pKa = 4.25RR903 pKa = 11.84YY904 pKa = 9.6LIIKK908 pKa = 10.18KK909 pKa = 9.39EE910 pKa = 4.04LQSEE914 pKa = 4.63LSRR917 pKa = 11.84LIDD920 pKa = 3.37QNEE923 pKa = 4.01NLNVQFNNAKK933 pKa = 10.3NLTTLQKK940 pKa = 11.17EE941 pKa = 4.13EE942 pKa = 4.52MIRR945 pKa = 11.84SLASDD950 pKa = 3.51FAIAYY955 pKa = 7.96KK956 pKa = 10.34PSNSYY961 pKa = 10.23EE962 pKa = 3.94QLQKK966 pKa = 10.5SGEE969 pKa = 4.2IMRR972 pKa = 11.84HH973 pKa = 3.74VQRR976 pKa = 11.84AITEE980 pKa = 4.08NEE982 pKa = 3.92KK983 pKa = 10.72KK984 pKa = 10.08IEE986 pKa = 4.45SIQGSLKK993 pKa = 9.69QLKK996 pKa = 7.76TVYY999 pKa = 10.18NSCCEE1004 pKa = 4.09TIMNNINKK1012 pKa = 9.56LDD1014 pKa = 3.6NTLRR1018 pKa = 11.84FAKK1021 pKa = 10.23KK1022 pKa = 10.24EE1023 pKa = 3.85KK1024 pKa = 10.76DD1025 pKa = 3.33PLLLSNFDD1033 pKa = 3.85SVTDD1037 pKa = 3.71NGLVEE1042 pKa = 4.81PNQLMDD1048 pKa = 5.06DD1049 pKa = 5.21LIDD1052 pKa = 4.19FSNTFDD1058 pKa = 4.49NISNEE1063 pKa = 3.85QLDD1066 pKa = 3.87DD1067 pKa = 4.77FIYY1070 pKa = 11.06EE1071 pKa = 4.07NMDD1074 pKa = 3.5RR1075 pKa = 11.84NIDD1078 pKa = 3.74FEE1080 pKa = 5.18FEE1082 pKa = 3.97GFNNDD1087 pKa = 4.72FVDD1090 pKa = 3.62IDD1092 pKa = 4.19AKK1094 pKa = 11.61VMDD1097 pKa = 4.32SMSAFSVNDD1106 pKa = 3.61LDD1108 pKa = 5.67IEE1110 pKa = 4.44TLVPDD1115 pKa = 3.65RR1116 pKa = 11.84TSNFSSLLDD1125 pKa = 3.59EE1126 pKa = 5.74DD1127 pKa = 5.16LFEE1130 pKa = 5.41SSGDD1134 pKa = 3.6FSLDD1138 pKa = 3.42YY1139 pKa = 11.31

Molecular weight: 130.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P47414|RS5_MYCGE 30S ribosomal protein S5 OS=Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) OX=243273 GN=rpsE PE=3 SV=1
MM1 pKa = 7.61AKK3 pKa = 10.14KK4 pKa = 10.1SLKK7 pKa = 10.15VKK9 pKa = 9.97QSRR12 pKa = 11.84PNKK15 pKa = 9.88FSVRR19 pKa = 11.84DD20 pKa = 3.43YY21 pKa = 10.38TRR23 pKa = 11.84CLRR26 pKa = 11.84CGRR29 pKa = 11.84ARR31 pKa = 11.84AVLSHH36 pKa = 6.81FGVCRR41 pKa = 11.84LCFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 10.57AGAIPGVKK57 pKa = 9.35KK58 pKa = 10.94ASWW61 pKa = 3.03

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

483

0

483

177065

37

1805

366.6

41.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.566 ± 0.083

0.825 ± 0.036

4.915 ± 0.081

5.64 ± 0.138

6.158 ± 0.107

4.626 ± 0.095

1.576 ± 0.049

8.261 ± 0.125

9.502 ± 0.139

10.684 ± 0.137

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.528 ± 0.041

7.523 ± 0.116

2.989 ± 0.087

4.736 ± 0.106

3.092 ± 0.075

6.658 ± 0.116

5.397 ± 0.079

6.11 ± 0.098

0.972 ± 0.038

3.241 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski