Synechococcus phage S-MbCM100
Average proteome isoelectric point is 5.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 210 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|V5UTV0|V5UTV0_9CAUD Precursor of head vertex protein OS=Synechococcus phage S-MbCM100 OX=1340812 GN=S-MbCM100_112 PE=4 SV=1
MM1 pKa = 7.63 LIALAVLVTVGAFILGIVVSWLAKK25 pKa = 10.42 GYY27 pKa = 10.55 VEE29 pKa = 6.23 DD30 pKa = 4.57 YY31 pKa = 10.73 IEE33 pKa = 3.99 NAAYY37 pKa = 10.08 SKK39 pKa = 10.91 SVTHH43 pKa = 6.95 PEE45 pKa = 3.96 MFDD48 pKa = 3.29 EE49 pKa = 4.86 EE50 pKa = 5.12 GNMIRR55 pKa = 11.84 DD56 pKa = 3.59 EE57 pKa = 4.88 LIYY60 pKa = 10.22 IRR62 pKa = 11.84 PDD64 pKa = 2.74 IQYY67 pKa = 10.35 WDD69 pKa = 5.0 DD70 pKa = 4.62 LPDD73 pKa = 5.36 DD74 pKa = 4.69 EE75 pKa = 7.73 DD76 pKa = 4.15 DD77 pKa = 3.69
Molecular weight: 8.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.745
IPC_protein 3.694
Toseland 3.49
ProMoST 3.808
Dawson 3.694
Bjellqvist 3.91
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.401
Solomon 3.668
Lehninger 3.63
Nozaki 3.821
DTASelect 4.037
Thurlkill 3.567
EMBOSS 3.656
Sillero 3.821
Patrickios 1.863
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.772
Protein with the highest isoelectric point:
>tr|V5UU11|V5UU11_9CAUD Photosystem II OS=Synechococcus phage S-MbCM100 OX=1340812 GN=S-MbCM100_192 PE=3 SV=1
MM1 pKa = 7.13 NTLQMVKK8 pKa = 10.33 KK9 pKa = 10.15 QINKK13 pKa = 9.94 ASALHH18 pKa = 5.9 NAQISHH24 pKa = 5.52 TSYY27 pKa = 10.84 RR28 pKa = 11.84 GVEE31 pKa = 4.01 YY32 pKa = 8.83 NTRR35 pKa = 11.84 CVEE38 pKa = 4.18 SKK40 pKa = 8.33 EE41 pKa = 4.27 THH43 pKa = 5.12 GTFCYY48 pKa = 9.92 RR49 pKa = 11.84 GRR51 pKa = 11.84 TYY53 pKa = 11.35 SKK55 pKa = 11.12
Molecular weight: 6.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.207
IPC2_protein 9.297
IPC_protein 9.326
Toseland 9.736
ProMoST 9.677
Dawson 10.014
Bjellqvist 9.765
Wikipedia 10.204
Rodwell 10.292
Grimsley 10.101
Solomon 10.072
Lehninger 10.028
Nozaki 9.838
DTASelect 9.721
Thurlkill 9.838
EMBOSS 10.16
Sillero 9.955
Patrickios 7.497
IPC_peptide 10.058
IPC2_peptide 8.682
IPC2.peptide.svr19 8.161
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
210
0
210
53288
35
6064
253.8
28.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.908 ± 0.212
0.878 ± 0.088
6.748 ± 0.125
5.893 ± 0.27
4.335 ± 0.106
7.936 ± 0.325
1.315 ± 0.107
6.45 ± 0.197
5.536 ± 0.365
7.264 ± 0.152
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.044 ± 0.207
5.802 ± 0.131
3.894 ± 0.13
3.639 ± 0.099
3.86 ± 0.167
7.677 ± 0.209
7.809 ± 0.37
6.746 ± 0.195
1.145 ± 0.088
4.121 ± 0.126
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here