Proteus phage pPM_01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4SK66|A0A0B4SK66_9CAUD Uncharacterized protein OS=Proteus phage pPM_01 OX=1567485 GN=pPM01_0016 PE=4 SV=1
MM1 pKa = 7.87AEE3 pKa = 3.8LAYY6 pKa = 9.35TKK8 pKa = 10.86ASIFQSIIDD17 pKa = 4.32DD18 pKa = 4.09VNTGLPKK25 pKa = 10.07GTSLDD30 pKa = 3.52TRR32 pKa = 11.84GDD34 pKa = 3.49FEE36 pKa = 4.21NQLRR40 pKa = 11.84EE41 pKa = 4.14LFEE44 pKa = 4.86EE45 pKa = 4.3NNLMNAPEE53 pKa = 5.06CIGVDD58 pKa = 3.55YY59 pKa = 10.8FGEE62 pKa = 3.92AWEE65 pKa = 4.39IVQSNEE71 pKa = 3.39FDD73 pKa = 3.89SVEE76 pKa = 4.27LEE78 pKa = 4.19SPLDD82 pKa = 3.71FSGCEE87 pKa = 3.76SSLNCLMMEE96 pKa = 4.59ANALIQASWRR106 pKa = 11.84EE107 pKa = 3.85QVEE110 pKa = 4.23AYY112 pKa = 9.77IEE114 pKa = 4.01EE115 pKa = 4.16LAEE118 pKa = 4.01ALEE121 pKa = 4.41EE122 pKa = 4.12VVEE125 pKa = 4.53GCEE128 pKa = 3.82EE129 pKa = 4.15FGTIDD134 pKa = 3.57EE135 pKa = 4.42LVITGGCRR143 pKa = 11.84FGHH146 pKa = 6.31ISHH149 pKa = 6.8LRR151 pKa = 11.84EE152 pKa = 3.37FDD154 pKa = 3.58AGDD157 pKa = 3.68FSVCMWAPNEE167 pKa = 4.29DD168 pKa = 3.83GEE170 pKa = 5.08AEE172 pKa = 4.02VYY174 pKa = 10.85YY175 pKa = 10.65NFRR178 pKa = 11.84GLEE181 pKa = 3.89LRR183 pKa = 11.84GVVYY187 pKa = 10.63HH188 pKa = 6.78GG189 pKa = 3.79

Molecular weight: 21.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4SK12|A0A0B4SK12_9CAUD Putative prohead protease ClpP OS=Proteus phage pPM_01 OX=1567485 GN=pPM01_0057 PE=4 SV=1
MM1 pKa = 7.73AISVEE6 pKa = 4.48TVGVNALKK14 pKa = 10.61LYY16 pKa = 10.24FDD18 pKa = 5.19GMPKK22 pKa = 7.97MTARR26 pKa = 11.84SMRR29 pKa = 11.84LAINTVVRR37 pKa = 11.84RR38 pKa = 11.84SGMKK42 pKa = 10.15ALRR45 pKa = 11.84DD46 pKa = 3.44GMLAEE51 pKa = 4.25ISFPRR56 pKa = 11.84GYY58 pKa = 11.04LKK60 pKa = 10.74GDD62 pKa = 3.49RR63 pKa = 11.84LKK65 pKa = 10.44VARR68 pKa = 11.84YY69 pKa = 9.59ASDD72 pKa = 3.32NKK74 pKa = 11.02LEE76 pKa = 3.98ATILARR82 pKa = 11.84KK83 pKa = 9.16RR84 pKa = 11.84ATSLARR90 pKa = 11.84FATSGTMGKK99 pKa = 9.84SGVTVKK105 pKa = 9.95VRR107 pKa = 11.84RR108 pKa = 11.84GRR110 pKa = 11.84TTFLKK115 pKa = 10.67KK116 pKa = 10.69AFLVKK121 pKa = 10.5LKK123 pKa = 10.55AGASMSEE130 pKa = 4.11DD131 pKa = 3.38NYY133 pKa = 10.3NTGLAVRR140 pKa = 11.84LSPGEE145 pKa = 3.89KK146 pKa = 10.32LNNKK150 pKa = 9.41ASAHH154 pKa = 5.95KK155 pKa = 10.07AWLVPGRR162 pKa = 11.84VALLYY167 pKa = 10.59APSVNQVMEE176 pKa = 4.35TTAGKK181 pKa = 10.34VSPQIAKK188 pKa = 10.1LVEE191 pKa = 4.18GEE193 pKa = 4.16FFRR196 pKa = 11.84QFEE199 pKa = 4.15RR200 pKa = 11.84LSNGKK205 pKa = 9.24

Molecular weight: 22.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

17539

37

1445

250.6

28.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.555 ± 0.513

0.878 ± 0.102

6.631 ± 0.255

7.013 ± 0.22

4.156 ± 0.216

7.212 ± 0.263

1.545 ± 0.123

6.044 ± 0.159

6.266 ± 0.35

7.674 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.731 ± 0.119

4.744 ± 0.159

4.362 ± 0.375

3.227 ± 0.227

5.553 ± 0.197

6.506 ± 0.288

5.918 ± 0.26

7.212 ± 0.335

1.397 ± 0.134

3.375 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski