Beihai sobemo-like virus 6
Average proteome isoelectric point is 7.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KEB9|A0A1L3KEB9_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 6 OX=1922703 PE=4 SV=1
MM1 pKa = 7.88 AICDD5 pKa = 4.14 SVSACAFTAFIWGPFAAVTFFLGLLFKK32 pKa = 10.61 IAGSFVSFLDD42 pKa = 4.8 AITEE46 pKa = 4.43 GPLKK50 pKa = 10.8 VYY52 pKa = 10.41 AALLLAVVTSGGAAYY67 pKa = 10.12 LYY69 pKa = 9.87 YY70 pKa = 10.79 RR71 pKa = 11.84 MRR73 pKa = 11.84 ILSRR77 pKa = 11.84 VVLLDD82 pKa = 3.4 VAEE85 pKa = 4.61 EE86 pKa = 3.99 PVGPYY91 pKa = 9.67 RR92 pKa = 11.84 GSFGRR97 pKa = 11.84 LFFRR101 pKa = 11.84 QSPGDD106 pKa = 3.63 KK107 pKa = 10.4 VFEE110 pKa = 4.31 AVPEE114 pKa = 3.97 AMVMSSSGEE123 pKa = 3.9 LGTAQVTYY131 pKa = 10.84 KK132 pKa = 10.42 LQPLGGDD139 pKa = 3.55 GDD141 pKa = 4.44 EE142 pKa = 4.37 STLAYY147 pKa = 10.38 SSPPRR152 pKa = 11.84 VALKK156 pKa = 10.42 LPPFMFEE163 pKa = 3.68 IGLMTGDD170 pKa = 3.64 GMMTILGIGFRR181 pKa = 11.84 AGDD184 pKa = 3.64 YY185 pKa = 10.78 LVTARR190 pKa = 11.84 HH191 pKa = 6.19 LFHH194 pKa = 7.01 SRR196 pKa = 11.84 NKK198 pKa = 9.5 VGFGARR204 pKa = 11.84 SCFIRR209 pKa = 11.84 RR210 pKa = 11.84 GPISVEE216 pKa = 3.94 VQLTNPIFTNGKK228 pKa = 8.31 SNEE231 pKa = 4.02 FEE233 pKa = 4.3 SMSWMDD239 pKa = 3.02 ICAYY243 pKa = 9.89 LVPPGEE249 pKa = 4.04 WAALGARR256 pKa = 11.84 SLRR259 pKa = 11.84 TTEE262 pKa = 4.52 LSSEE266 pKa = 4.08 GDD268 pKa = 3.59 GTVEE272 pKa = 4.01 VYY274 pKa = 10.52 GRR276 pKa = 11.84 PGAQLMVSSGQLVNDD291 pKa = 3.19 VKK293 pKa = 10.84 MEE295 pKa = 3.93 VEE297 pKa = 4.21 RR298 pKa = 11.84 GVLSHH303 pKa = 6.65 TASTVGTFSGSPLVMMQGGAYY324 pKa = 9.27 KK325 pKa = 10.48 LVGMHH330 pKa = 6.58 IAGGVDD336 pKa = 3.09 VNYY339 pKa = 10.35 AVSAGGVRR347 pKa = 11.84 RR348 pKa = 11.84 MLKK351 pKa = 10.52 ALDD354 pKa = 3.59 FGPRR358 pKa = 11.84 SRR360 pKa = 11.84 AGVVWDD366 pKa = 3.7 ALRR369 pKa = 11.84 PQFHH373 pKa = 6.69 NEE375 pKa = 3.7 SKK377 pKa = 10.59 EE378 pKa = 3.89 EE379 pKa = 3.85 RR380 pKa = 11.84 RR381 pKa = 11.84 KK382 pKa = 10.42 QMEE385 pKa = 4.34 PKK387 pKa = 10.09 YY388 pKa = 10.25 SLWDD392 pKa = 3.64 EE393 pKa = 4.25 EE394 pKa = 4.16 EE395 pKa = 3.62 MRR397 pKa = 11.84 ARR399 pKa = 11.84 QDD401 pKa = 3.32 QEE403 pKa = 3.89 DD404 pKa = 4.23 CEE406 pKa = 4.7 RR407 pKa = 11.84 EE408 pKa = 3.93 LMAEE412 pKa = 4.15 VKK414 pKa = 10.13 DD415 pKa = 3.51 YY416 pKa = 11.17 HH417 pKa = 6.22 AAKK420 pKa = 10.5 LHH422 pKa = 5.5 GQGHH426 pKa = 7.27 LDD428 pKa = 3.1 WKK430 pKa = 10.12 IGKK433 pKa = 8.59 RR434 pKa = 11.84 QQRR437 pKa = 11.84 TEE439 pKa = 4.09 KK440 pKa = 10.2 KK441 pKa = 10.16 DD442 pKa = 3.18 AAASSKK448 pKa = 10.07 EE449 pKa = 3.6 RR450 pKa = 11.84 WADD453 pKa = 3.33 MMDD456 pKa = 3.73 DD457 pKa = 4.49 SIEE460 pKa = 4.08 EE461 pKa = 4.32 SPSRR465 pKa = 11.84 TFTDD469 pKa = 3.42 EE470 pKa = 3.9 EE471 pKa = 4.6 LEE473 pKa = 4.13 LAEE476 pKa = 4.43 RR477 pKa = 11.84 LYY479 pKa = 11.27 EE480 pKa = 4.32 RR481 pKa = 11.84 LSQMKK486 pKa = 9.84 RR487 pKa = 11.84 EE488 pKa = 4.1 KK489 pKa = 11.04 AKK491 pKa = 10.52 GVYY494 pKa = 9.82 NGWIDD499 pKa = 3.26 KK500 pKa = 10.84 SYY502 pKa = 9.35 VQGYY506 pKa = 9.45 CFLSKK511 pKa = 9.89 TKK513 pKa = 9.46 TYY515 pKa = 10.52 KK516 pKa = 10.9 ALFVLSRR523 pKa = 11.84 INRR526 pKa = 11.84 IQLWSWKK533 pKa = 8.98 DD534 pKa = 3.51 RR535 pKa = 11.84 SWIAMEE541 pKa = 4.51 EE542 pKa = 4.23 LLSHH546 pKa = 6.61 LPSVQEE552 pKa = 3.83 EE553 pKa = 4.37 DD554 pKa = 3.06 QGQEE558 pKa = 3.8 DD559 pKa = 4.03 RR560 pKa = 11.84 EE561 pKa = 4.31 EE562 pKa = 4.11 EE563 pKa = 4.38 TEE565 pKa = 4.1 GAEE568 pKa = 4.04 QQEE571 pKa = 4.46 ADD573 pKa = 4.06 AQEE576 pKa = 4.26 QPPSDD581 pKa = 4.11 SAQHH585 pKa = 5.96 EE586 pKa = 4.78 SPPGLKK592 pKa = 10.16 RR593 pKa = 11.84 SDD595 pKa = 4.05 SIAAAVWHH603 pKa = 6.28 NSAPLRR609 pKa = 11.84 YY610 pKa = 8.98 EE611 pKa = 3.77 EE612 pKa = 5.09 HH613 pKa = 6.72 SLSKK617 pKa = 10.56 PGLIGNLGAPRR628 pKa = 11.84 AAPRR632 pKa = 11.84 SDD634 pKa = 3.92 HH635 pKa = 6.93 PPHH638 pKa = 6.74 SFPFATGGEE647 pKa = 4.37 KK648 pKa = 10.17 FWADD652 pKa = 3.04 RR653 pKa = 11.84 RR654 pKa = 11.84 SQCRR658 pKa = 11.84 RR659 pKa = 11.84 PLEE662 pKa = 4.19 PRR664 pKa = 11.84 DD665 pKa = 3.96 LNPQIEE671 pKa = 4.66 GDD673 pKa = 3.7 MEE675 pKa = 4.36 DD676 pKa = 3.82 WMTKK680 pKa = 10.39 GDD682 pKa = 3.58 WNSWRR687 pKa = 11.84 RR688 pKa = 11.84 LKK690 pKa = 11.04 NVGAKK695 pKa = 8.91 TLLQMPQMQEE705 pKa = 3.38 YY706 pKa = 8.47 RR707 pKa = 11.84 QIMRR711 pKa = 11.84 AGNVRR716 pKa = 11.84 IWKK719 pKa = 9.61 DD720 pKa = 2.9 KK721 pKa = 9.3 KK722 pKa = 8.73 TRR724 pKa = 11.84 SSNVAYY730 pKa = 10.12 NANGAAHH737 pKa = 8.23 AYY739 pKa = 10.36 RR740 pKa = 11.84 IGTCEE745 pKa = 3.8 ASRR748 pKa = 11.84 GRR750 pKa = 11.84 PRR752 pKa = 11.84 EE753 pKa = 4.11 AKK755 pKa = 9.76 HH756 pKa = 5.75 VSEE759 pKa = 5.35 DD760 pKa = 4.31 FVDD763 pKa = 3.87 ALQDD767 pKa = 2.98 LWYY770 pKa = 10.24 EE771 pKa = 4.18 GFEE774 pKa = 4.46 EE775 pKa = 4.86 PGSLAKK781 pKa = 10.26 KK782 pKa = 8.93 VSGYY786 pKa = 10.88 ALPPSGPDD794 pKa = 2.96 AVKK797 pKa = 10.67 ASFIGQCGRR806 pKa = 11.84 QKK808 pKa = 10.5 PGNWDD813 pKa = 3.43 KK814 pKa = 11.34 LRR816 pKa = 11.84 EE817 pKa = 4.18 TPNFHH822 pKa = 5.52 TQVNRR827 pKa = 11.84 FYY829 pKa = 11.26 KK830 pKa = 10.28 EE831 pKa = 3.86 YY832 pKa = 10.2 TPSDD836 pKa = 3.49 PCTRR840 pKa = 11.84 SSIQDD845 pKa = 3.85 SIDD848 pKa = 3.61 HH849 pKa = 6.38 YY850 pKa = 10.92 LDD852 pKa = 3.62 NMDD855 pKa = 3.95 GAKK858 pKa = 10.01 SAGWSSRR865 pKa = 11.84 VRR867 pKa = 11.84 AGTKK871 pKa = 9.82 GVWSRR876 pKa = 11.84 NEE878 pKa = 3.71 DD879 pKa = 3.29 ARR881 pKa = 11.84 CVLTYY886 pKa = 10.93 CVMARR891 pKa = 11.84 LALRR895 pKa = 11.84 IAEE898 pKa = 4.32 GDD900 pKa = 3.61 NIHH903 pKa = 6.41 WLKK906 pKa = 11.19 AEE908 pKa = 4.27 DD909 pKa = 4.0 MVSLGLKK916 pKa = 10.43 DD917 pKa = 3.4 PVEE920 pKa = 4.33 IFTKK924 pKa = 10.55 TEE926 pKa = 3.39 AHH928 pKa = 6.84 DD929 pKa = 3.77 EE930 pKa = 4.31 KK931 pKa = 10.92 KK932 pKa = 11.14 VKK934 pKa = 10.3 DD935 pKa = 3.85 EE936 pKa = 3.74 RR937 pKa = 11.84 WRR939 pKa = 11.84 LIWVVSMIDD948 pKa = 4.16 SVCQDD953 pKa = 2.67 IAHH956 pKa = 6.94 RR957 pKa = 11.84 HH958 pKa = 4.22 QNKK961 pKa = 8.44 TDD963 pKa = 3.02 IYY965 pKa = 10.13 AYY967 pKa = 10.4 AAGKK971 pKa = 9.76 LNCQAVGLGHH981 pKa = 7.2 HH982 pKa = 7.25 DD983 pKa = 3.57 NGVKK987 pKa = 10.45 RR988 pKa = 11.84 IGEE991 pKa = 4.09 MLEE994 pKa = 4.43 EE995 pKa = 4.15 ISKK998 pKa = 10.54 KK999 pKa = 8.39 YY1000 pKa = 9.31 PRR1002 pKa = 11.84 WGTAGQVAYY1011 pKa = 10.57 DD1012 pKa = 4.48 DD1013 pKa = 4.65 FQSSDD1018 pKa = 2.94 ASGWDD1023 pKa = 3.37 LSVSRR1028 pKa = 11.84 DD1029 pKa = 3.52 AIYY1032 pKa = 10.63 FDD1034 pKa = 3.47 AEE1036 pKa = 3.75 RR1037 pKa = 11.84 RR1038 pKa = 11.84 IALSTRR1044 pKa = 11.84 ACPAYY1049 pKa = 10.68 NDD1051 pKa = 4.4 LLWAEE1056 pKa = 4.35 AACNSAHH1063 pKa = 5.7 VVVIGTAMWTFEE1075 pKa = 4.14 WFGITASGIPSTSAQNSSIRR1095 pKa = 11.84 SFTAQACGATKK1106 pKa = 8.71 QTAAGDD1112 pKa = 4.19 DD1113 pKa = 3.98 LVHH1116 pKa = 6.5 TGSLDD1121 pKa = 3.4 IEE1123 pKa = 4.47 LLASTGCLTKK1133 pKa = 10.53 EE1134 pKa = 3.75 GSEE1137 pKa = 3.94 RR1138 pKa = 11.84 SSGPEE1143 pKa = 3.77 GPVEE1147 pKa = 4.17 FTSHH1151 pKa = 5.02 VFKK1154 pKa = 10.99 KK1155 pKa = 10.77 VDD1157 pKa = 3.97 GVWTATFNNLVKK1169 pKa = 10.17 MLAHH1173 pKa = 6.9 LDD1175 pKa = 3.37 LRR1177 pKa = 11.84 RR1178 pKa = 11.84 PDD1180 pKa = 3.81 GEE1182 pKa = 4.42 APSSDD1187 pKa = 3.53 MVSGMRR1193 pKa = 11.84 FALRR1197 pKa = 11.84 HH1198 pKa = 5.44 TPLADD1203 pKa = 3.19 RR1204 pKa = 11.84 VFCQVCDD1211 pKa = 3.47 RR1212 pKa = 11.84 MGWSVSQPVDD1222 pKa = 3.72 VAWEE1226 pKa = 3.84
Molecular weight: 136.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.82
IPC2_protein 5.779
IPC_protein 5.83
Toseland 6.173
ProMoST 6.122
Dawson 6.071
Bjellqvist 6.097
Wikipedia 6.084
Rodwell 6.059
Grimsley 6.313
Solomon 6.071
Lehninger 6.071
Nozaki 6.338
DTASelect 6.532
Thurlkill 6.532
EMBOSS 6.491
Sillero 6.44
Patrickios 4.317
IPC_peptide 6.097
IPC2_peptide 6.415
IPC2.peptide.svr19 6.421
Protein with the highest isoelectric point:
>tr|A0A1L3KEB9|A0A1L3KEB9_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 6 OX=1922703 PE=4 SV=1
MM1 pKa = 7.13 RR2 pKa = 11.84 TGRR5 pKa = 11.84 RR6 pKa = 11.84 QLRR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AALPRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 RR21 pKa = 11.84 ARR23 pKa = 11.84 AATALRR29 pKa = 11.84 PTNRR33 pKa = 11.84 THH35 pKa = 6.1 ATNVLATGVGAAPKK49 pKa = 10.01 RR50 pKa = 11.84 AFGCTTGHH58 pKa = 7.1 TLACWDD64 pKa = 4.01 AKK66 pKa = 10.63 HH67 pKa = 6.08 PHH69 pKa = 6.69 HH70 pKa = 7.02 LPLPRR75 pKa = 11.84 AVGPYY80 pKa = 8.84 TVIRR84 pKa = 11.84 ATKK87 pKa = 8.43 RR88 pKa = 11.84 VQVSTVANLIGTYY101 pKa = 9.5 QRR103 pKa = 11.84 VAVTSPHH110 pKa = 4.99 QGAWSEE116 pKa = 4.35 VIMVSDD122 pKa = 3.95 VNNLGLINSTNNAHH136 pKa = 6.61 SISVDD141 pKa = 3.4 LDD143 pKa = 3.5 GMGDD147 pKa = 3.51 SATLVPSALSVQIMCPTALQTASGIIYY174 pKa = 9.82 AGVMNTQAQLGGRR187 pKa = 11.84 SEE189 pKa = 4.24 TWDD192 pKa = 3.04 SYY194 pKa = 9.96 MNKK197 pKa = 9.1 FVQFQSPRR205 pKa = 11.84 LLAASKK211 pKa = 10.22 LALRR215 pKa = 11.84 GTQINSYY222 pKa = 8.56 PLNMAEE228 pKa = 3.96 VSKK231 pKa = 10.89 FSSLDD236 pKa = 3.11 RR237 pKa = 11.84 AGDD240 pKa = 3.76 VDD242 pKa = 4.56 FTWSNDD248 pKa = 3.07 KK249 pKa = 11.35 YY250 pKa = 11.05 EE251 pKa = 4.38 STGWAPIVIYY261 pKa = 10.43 NPQGATLEE269 pKa = 4.01 LLITVEE275 pKa = 3.69 YY276 pKa = 10.11 RR277 pKa = 11.84 VRR279 pKa = 11.84 FDD281 pKa = 4.28 LDD283 pKa = 3.4 HH284 pKa = 7.03 PASASHH290 pKa = 6.07 KK291 pKa = 8.3 HH292 pKa = 5.85 HH293 pKa = 7.45 PIASDD298 pKa = 3.7 GMWDD302 pKa = 3.26 KK303 pKa = 10.5 MVRR306 pKa = 11.84 QASSLGHH313 pKa = 5.61 GVMDD317 pKa = 4.11 IADD320 pKa = 3.69 VVANAGMAVGRR331 pKa = 11.84 AMTVGNKK338 pKa = 10.02 LMGAGRR344 pKa = 11.84 SMLALAAA351 pKa = 4.51
Molecular weight: 37.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.633
IPC_protein 10.452
Toseland 10.467
ProMoST 10.218
Dawson 10.628
Bjellqvist 10.35
Wikipedia 10.847
Rodwell 10.789
Grimsley 10.687
Solomon 10.716
Lehninger 10.672
Nozaki 10.467
DTASelect 10.35
Thurlkill 10.496
EMBOSS 10.877
Sillero 10.555
Patrickios 10.452
IPC_peptide 10.716
IPC2_peptide 9.385
IPC2.peptide.svr19 8.566
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1577
351
1226
788.5
87.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.146 ± 1.317
1.458 ± 0.297
5.77 ± 0.597
5.834 ± 2.03
3.234 ± 0.75
8.053 ± 0.178
2.6 ± 0.403
3.678 ± 0.153
4.566 ± 0.845
7.673 ± 0.29
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.297 ± 0.34
3.044 ± 0.746
4.566 ± 0.004
3.741 ± 0.018
7.166 ± 0.54
8.18 ± 0.24
5.073 ± 1.149
6.785 ± 0.587
2.41 ± 0.345
2.727 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here