Lake Sarah-associated circular molecule 2

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A126G9F6|A0A126G9F6_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 2 OX=1685727 PE=3 SV=1
MM1 pKa = 6.27STRR4 pKa = 11.84RR5 pKa = 11.84IVFTKK10 pKa = 10.85NNWTEE15 pKa = 3.35EE16 pKa = 4.2DD17 pKa = 3.54YY18 pKa = 10.92RR19 pKa = 11.84ALMEE23 pKa = 4.7EE24 pKa = 4.39PLFSYY29 pKa = 10.11LIIGKK34 pKa = 9.6EE35 pKa = 3.76IGEE38 pKa = 4.53KK39 pKa = 9.31GTPHH43 pKa = 5.85LQGYY47 pKa = 10.53AEE49 pKa = 4.08FTKK52 pKa = 10.26KK53 pKa = 10.53AKK55 pKa = 10.55YY56 pKa = 9.33GALAKK61 pKa = 9.83KK62 pKa = 10.36YY63 pKa = 10.45KK64 pKa = 8.33MHH66 pKa = 6.98CEE68 pKa = 4.11VSRR71 pKa = 11.84GTQDD75 pKa = 3.26EE76 pKa = 4.52AIKK79 pKa = 11.06YY80 pKa = 9.22CMKK83 pKa = 10.91DD84 pKa = 2.34GDD86 pKa = 4.12YY87 pKa = 11.42AEE89 pKa = 5.36KK90 pKa = 9.67GTKK93 pKa = 9.99KK94 pKa = 10.56LDD96 pKa = 3.52GASAEE101 pKa = 4.23KK102 pKa = 10.65NRR104 pKa = 11.84WEE106 pKa = 4.05QARR109 pKa = 11.84VSAKK113 pKa = 10.02EE114 pKa = 3.74GRR116 pKa = 11.84LDD118 pKa = 4.74DD119 pKa = 4.18VPADD123 pKa = 3.34IYY125 pKa = 10.5IRR127 pKa = 11.84CYY129 pKa = 9.69RR130 pKa = 11.84TLKK133 pKa = 10.65EE134 pKa = 3.75IAKK137 pKa = 10.64DD138 pKa = 3.61NMPKK142 pKa = 10.24PEE144 pKa = 4.58SLTEE148 pKa = 4.49LKK150 pKa = 10.76NLWIYY155 pKa = 10.85GPAGSGKK162 pKa = 8.01TRR164 pKa = 11.84LADD167 pKa = 4.44AIVPISYY174 pKa = 10.61SKK176 pKa = 11.09NCNKK180 pKa = 9.25WWDD183 pKa = 4.07GYY185 pKa = 9.2QNEE188 pKa = 4.76PGVIINDD195 pKa = 3.35VGKK198 pKa = 8.2EE199 pKa = 3.83HH200 pKa = 6.96SVLGHH205 pKa = 6.24HH206 pKa = 6.58FKK208 pKa = 10.96LWGEE212 pKa = 3.96HH213 pKa = 5.72RR214 pKa = 11.84PFIAEE219 pKa = 4.08TKK221 pKa = 10.27GGAIHH226 pKa = 7.08IRR228 pKa = 11.84PQRR231 pKa = 11.84VIITSQYY238 pKa = 10.87SLTQIWEE245 pKa = 4.2DD246 pKa = 3.73EE247 pKa = 4.24EE248 pKa = 4.42TRR250 pKa = 11.84DD251 pKa = 4.2ALARR255 pKa = 11.84RR256 pKa = 11.84YY257 pKa = 9.61KK258 pKa = 10.44VLHH261 pKa = 6.17LLGNLDD267 pKa = 4.57LEE269 pKa = 5.2DD270 pKa = 3.87IDD272 pKa = 6.16RR273 pKa = 11.84ISKK276 pKa = 10.54DD277 pKa = 3.43YY278 pKa = 11.45AA279 pKa = 4.18

Molecular weight: 32.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A126G9F6|A0A126G9F6_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 2 OX=1685727 PE=3 SV=1
MM1 pKa = 6.27STRR4 pKa = 11.84RR5 pKa = 11.84IVFTKK10 pKa = 10.85NNWTEE15 pKa = 3.35EE16 pKa = 4.2DD17 pKa = 3.54YY18 pKa = 10.92RR19 pKa = 11.84ALMEE23 pKa = 4.7EE24 pKa = 4.39PLFSYY29 pKa = 10.11LIIGKK34 pKa = 9.6EE35 pKa = 3.76IGEE38 pKa = 4.53KK39 pKa = 9.31GTPHH43 pKa = 5.85LQGYY47 pKa = 10.53AEE49 pKa = 4.08FTKK52 pKa = 10.26KK53 pKa = 10.53AKK55 pKa = 10.55YY56 pKa = 9.33GALAKK61 pKa = 9.83KK62 pKa = 10.36YY63 pKa = 10.45KK64 pKa = 8.33MHH66 pKa = 6.98CEE68 pKa = 4.11VSRR71 pKa = 11.84GTQDD75 pKa = 3.26EE76 pKa = 4.52AIKK79 pKa = 11.06YY80 pKa = 9.22CMKK83 pKa = 10.91DD84 pKa = 2.34GDD86 pKa = 4.12YY87 pKa = 11.42AEE89 pKa = 5.36KK90 pKa = 9.67GTKK93 pKa = 9.99KK94 pKa = 10.56LDD96 pKa = 3.52GASAEE101 pKa = 4.23KK102 pKa = 10.65NRR104 pKa = 11.84WEE106 pKa = 4.05QARR109 pKa = 11.84VSAKK113 pKa = 10.02EE114 pKa = 3.74GRR116 pKa = 11.84LDD118 pKa = 4.74DD119 pKa = 4.18VPADD123 pKa = 3.34IYY125 pKa = 10.5IRR127 pKa = 11.84CYY129 pKa = 9.69RR130 pKa = 11.84TLKK133 pKa = 10.65EE134 pKa = 3.75IAKK137 pKa = 10.64DD138 pKa = 3.61NMPKK142 pKa = 10.24PEE144 pKa = 4.58SLTEE148 pKa = 4.49LKK150 pKa = 10.76NLWIYY155 pKa = 10.85GPAGSGKK162 pKa = 8.01TRR164 pKa = 11.84LADD167 pKa = 4.44AIVPISYY174 pKa = 10.61SKK176 pKa = 11.09NCNKK180 pKa = 9.25WWDD183 pKa = 4.07GYY185 pKa = 9.2QNEE188 pKa = 4.76PGVIINDD195 pKa = 3.35VGKK198 pKa = 8.2EE199 pKa = 3.83HH200 pKa = 6.96SVLGHH205 pKa = 6.24HH206 pKa = 6.58FKK208 pKa = 10.96LWGEE212 pKa = 3.96HH213 pKa = 5.72RR214 pKa = 11.84PFIAEE219 pKa = 4.08TKK221 pKa = 10.27GGAIHH226 pKa = 7.08IRR228 pKa = 11.84PQRR231 pKa = 11.84VIITSQYY238 pKa = 10.87SLTQIWEE245 pKa = 4.2DD246 pKa = 3.73EE247 pKa = 4.24EE248 pKa = 4.42TRR250 pKa = 11.84DD251 pKa = 4.2ALARR255 pKa = 11.84RR256 pKa = 11.84YY257 pKa = 9.61KK258 pKa = 10.44VLHH261 pKa = 6.17LLGNLDD267 pKa = 4.57LEE269 pKa = 5.2DD270 pKa = 3.87IDD272 pKa = 6.16RR273 pKa = 11.84ISKK276 pKa = 10.54DD277 pKa = 3.43YY278 pKa = 11.45AA279 pKa = 4.18

Molecular weight: 32.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

279

279

279

279.0

32.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.527 ± 0.0

1.434 ± 0.0

6.452 ± 0.0

8.602 ± 0.0

1.792 ± 0.0

7.527 ± 0.0

2.867 ± 0.0

7.527 ± 0.0

10.036 ± 0.0

7.527 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.792 ± 0.0

3.584 ± 0.0

3.584 ± 0.0

2.509 ± 0.0

6.093 ± 0.0

4.659 ± 0.0

5.018 ± 0.0

3.584 ± 0.0

2.509 ± 0.0

5.376 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski