Arthrobacter oryzae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3770 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N0C4G7|A0A3N0C4G7_9MICC DivIVA domain-containing protein OS=Arthrobacter oryzae OX=409290 GN=D7003_05885 PE=4 SV=1
MM1 pKa = 7.59AGVAALSASVLLGTVLSGCEE21 pKa = 3.84YY22 pKa = 10.42EE23 pKa = 5.01YY24 pKa = 11.37DD25 pKa = 4.46DD26 pKa = 6.21GPWDD30 pKa = 4.47DD31 pKa = 5.58DD32 pKa = 4.26FPAAAAPAATGAAIPPVLPQQAGLNQPLSGDD63 pKa = 4.09EE64 pKa = 3.98LDD66 pKa = 4.84DD67 pKa = 3.83WVDD70 pKa = 4.2DD71 pKa = 4.04VLPDD75 pKa = 3.46TDD77 pKa = 4.69HH78 pKa = 7.54DD79 pKa = 4.29VLHH82 pKa = 6.57TSSGSVDD89 pKa = 3.18ASDD92 pKa = 4.13PRR94 pKa = 11.84SEE96 pKa = 4.28TTTALPGGSYY106 pKa = 10.64SVTLACRR113 pKa = 11.84SADD116 pKa = 2.91RR117 pKa = 11.84VFFTVRR123 pKa = 11.84DD124 pKa = 3.53ADD126 pKa = 3.91MVLLDD131 pKa = 4.08LSLRR135 pKa = 11.84CGTSAVNIVFLPTEE149 pKa = 4.24EE150 pKa = 4.3VLTIQIAGQAPANYY164 pKa = 10.25AYY166 pKa = 9.99MVDD169 pKa = 3.69RR170 pKa = 11.84AA171 pKa = 3.88

Molecular weight: 17.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N0C4F2|A0A3N0C4F2_9MICC CinA family protein OS=Arthrobacter oryzae OX=409290 GN=D7003_05485 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3770

0

3770

1198144

18

1834

317.8

34.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.262 ± 0.055

0.633 ± 0.01

5.678 ± 0.035

5.517 ± 0.043

3.188 ± 0.026

9.125 ± 0.042

2.11 ± 0.02

4.448 ± 0.03

2.73 ± 0.028

10.223 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.018 ± 0.016

2.434 ± 0.022

5.456 ± 0.028

3.079 ± 0.022

6.738 ± 0.046

5.841 ± 0.029

5.922 ± 0.028

8.165 ± 0.04

1.404 ± 0.016

2.031 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski