Mycobacterium phage Bobby

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Omegavirus; unclassified Omegavirus

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 220 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481VYU7|A0A481VYU7_9CAUD Uncharacterized protein OS=Mycobacterium phage Bobby OX=2530120 GN=220 PE=4 SV=1
MM1 pKa = 7.99DD2 pKa = 3.4VTLPPLTRR10 pKa = 11.84EE11 pKa = 4.14DD12 pKa = 5.34FEE14 pKa = 4.24FQYY17 pKa = 11.16DD18 pKa = 3.47AGHH21 pKa = 6.97TYY23 pKa = 10.9PFFEE27 pKa = 5.94DD28 pKa = 3.19EE29 pKa = 4.53NAWGLYY35 pKa = 10.22AYY37 pKa = 10.58GDD39 pKa = 3.46IDD41 pKa = 3.54PAEE44 pKa = 4.41FARR47 pKa = 11.84LANEE51 pKa = 3.57YY52 pKa = 10.38DD53 pKa = 3.49IEE55 pKa = 5.42IGGLNPGDD63 pKa = 3.67ATYY66 pKa = 9.18TADD69 pKa = 4.61DD70 pKa = 3.63VHH72 pKa = 6.31TRR74 pKa = 11.84WAARR78 pKa = 11.84VDD80 pKa = 3.85EE81 pKa = 6.02DD82 pKa = 4.21CLQWAPSGTPNAFPVAVLDD101 pKa = 3.94RR102 pKa = 4.56

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481VXR3|A0A481VXR3_9CAUD Uncharacterized protein OS=Mycobacterium phage Bobby OX=2530120 GN=172 PE=4 SV=1
MM1 pKa = 6.49THH3 pKa = 7.07RR4 pKa = 11.84GLEE7 pKa = 3.97RR8 pKa = 11.84AEE10 pKa = 3.92EE11 pKa = 3.95RR12 pKa = 11.84RR13 pKa = 11.84VQIAKK18 pKa = 10.48LIRR21 pKa = 11.84QGLTNKK27 pKa = 9.43EE28 pKa = 3.79VAQIVGVTDD37 pKa = 3.29RR38 pKa = 11.84TVNRR42 pKa = 11.84ARR44 pKa = 11.84EE45 pKa = 4.02RR46 pKa = 11.84KK47 pKa = 9.19GLKK50 pKa = 9.77KK51 pKa = 9.73PQPPRR56 pKa = 11.84ISEE59 pKa = 4.07EE60 pKa = 4.17TLSAGYY66 pKa = 10.86QMLLDD71 pKa = 4.0GCSYY75 pKa = 11.69AEE77 pKa = 3.99VARR80 pKa = 11.84TLGHH84 pKa = 6.86SRR86 pKa = 11.84DD87 pKa = 2.93TWRR90 pKa = 11.84RR91 pKa = 11.84KK92 pKa = 10.15LPGFAWSHH100 pKa = 4.87VEE102 pKa = 3.63CGRR105 pKa = 11.84FRR107 pKa = 11.84KK108 pKa = 9.42LQEE111 pKa = 4.09RR112 pKa = 11.84YY113 pKa = 9.93EE114 pKa = 4.26GLLL117 pKa = 3.55

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

220

0

220

34533

39

1631

157.0

17.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.002 ± 0.291

1.494 ± 0.146

6.692 ± 0.182

6.973 ± 0.226

3.159 ± 0.096

8.444 ± 0.298

2.325 ± 0.112

4.798 ± 0.125

4.002 ± 0.183

8.14 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.317 ± 0.09

3.342 ± 0.109

5.288 ± 0.164

3.177 ± 0.152

6.854 ± 0.221

5.357 ± 0.144

5.531 ± 0.218

6.791 ± 0.186

2.308 ± 0.121

3.006 ± 0.11
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski