Vibrio phage vB_ValS_PJ32

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G8LPF3|A0A7G8LPF3_9CAUD Uncharacterized protein OS=Vibrio phage vB_ValS_PJ32 OX=2759211 PE=4 SV=1
MM1 pKa = 7.84FEE3 pKa = 4.9RR4 pKa = 11.84NDD6 pKa = 3.51DD7 pKa = 3.98LFFVKK12 pKa = 10.25PVNYY16 pKa = 9.77TDD18 pKa = 5.34DD19 pKa = 3.78NGEE22 pKa = 4.28TIYY25 pKa = 10.25TALTAEE31 pKa = 4.82FGDD34 pKa = 3.68GLNRR38 pKa = 11.84INTTIFPDD46 pKa = 3.22KK47 pKa = 10.49VGIMVFRR54 pKa = 11.84NTDD57 pKa = 3.44LDD59 pKa = 4.24PGLKK63 pKa = 9.96LDD65 pKa = 3.91IANGDD70 pKa = 3.85EE71 pKa = 4.46VPNPGPDD78 pKa = 3.3QEE80 pKa = 5.11VIALNFHH87 pKa = 6.07TLQSIDD93 pKa = 3.48AVIGRR98 pKa = 11.84LQYY101 pKa = 10.55IRR103 pKa = 11.84DD104 pKa = 3.66VMVDD108 pKa = 3.25RR109 pKa = 11.84TKK111 pKa = 11.05APHH114 pKa = 7.07ADD116 pKa = 3.5QTPEE120 pKa = 3.79QDD122 pKa = 3.3PDD124 pKa = 3.24QDD126 pKa = 3.56LTAVEE131 pKa = 4.41TKK133 pKa = 9.36LTYY136 pKa = 10.04EE137 pKa = 4.83AGDD140 pKa = 3.56NCPDD144 pKa = 3.58CEE146 pKa = 4.6GTLEE150 pKa = 4.61HH151 pKa = 6.95YY152 pKa = 10.39PDD154 pKa = 3.67EE155 pKa = 4.82CACAVSPPPCSACTDD170 pKa = 3.69APLSCCVCGWNEE182 pKa = 3.67KK183 pKa = 10.5DD184 pKa = 3.26QQ185 pKa = 3.85

Molecular weight: 20.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G8LPJ0|A0A7G8LPJ0_9CAUD Uncharacterized protein OS=Vibrio phage vB_ValS_PJ32 OX=2759211 PE=4 SV=1
MM1 pKa = 7.41VRR3 pKa = 11.84RR4 pKa = 11.84TWSPRR9 pKa = 11.84EE10 pKa = 3.76KK11 pKa = 10.76DD12 pKa = 3.2IVSALAGKK20 pKa = 10.2VPIIVIASLTGRR32 pKa = 11.84TVEE35 pKa = 4.31SVRR38 pKa = 11.84GYY40 pKa = 10.09CLRR43 pKa = 11.84RR44 pKa = 11.84QIAYY48 pKa = 9.06HH49 pKa = 4.44YY50 pKa = 9.81HH51 pKa = 6.03GKK53 pKa = 7.57TSRR56 pKa = 11.84RR57 pKa = 11.84AFSEE61 pKa = 4.01RR62 pKa = 11.84EE63 pKa = 3.79TRR65 pKa = 11.84IVGALAGKK73 pKa = 10.18VPIQIIEE80 pKa = 4.06ILTGRR85 pKa = 11.84GTTSIRR91 pKa = 11.84NKK93 pKa = 9.96AAALGKK99 pKa = 9.69SLEE102 pKa = 4.11YY103 pKa = 10.24FGKK106 pKa = 10.93GGDD109 pKa = 3.37ARR111 pKa = 11.84LGRR114 pKa = 11.84RR115 pKa = 11.84RR116 pKa = 11.84NPIEE120 pKa = 4.8KK121 pKa = 9.31IRR123 pKa = 11.84KK124 pKa = 8.21AKK126 pKa = 9.9TMLNEE131 pKa = 3.38GRR133 pKa = 11.84TRR135 pKa = 11.84AEE137 pKa = 3.57IMEE140 pKa = 4.28ATGLASSVISNILMGMTHH158 pKa = 6.21TEE160 pKa = 4.03VEE162 pKa = 4.12PQLTIKK168 pKa = 10.5KK169 pKa = 8.17EE170 pKa = 4.08CEE172 pKa = 3.87NIHH175 pKa = 6.95LIGEE179 pKa = 4.36VFRR182 pKa = 5.93

Molecular weight: 20.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

111

0

111

23608

47

1377

212.7

23.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.641 ± 0.345

1.199 ± 0.113

6.125 ± 0.185

6.955 ± 0.218

3.452 ± 0.103

6.294 ± 0.181

1.876 ± 0.126

6.307 ± 0.194

6.731 ± 0.281

9.399 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.108

5.18 ± 0.156

3.994 ± 0.214

3.689 ± 0.179

5.286 ± 0.195

5.778 ± 0.235

6.574 ± 0.216

6.197 ± 0.182

0.762 ± 0.077

3.38 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski