Poaceae-associated gemycircularvirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus poass1; Poaceae associated gemycircularvirus 1

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M4G0X9|A0A0M4G0X9_9VIRU Capsid protein OS=Poaceae-associated gemycircularvirus 1 OX=1708497 PE=4 SV=1
MM1 pKa = 6.68THH3 pKa = 7.33FIFSARR9 pKa = 11.84YY10 pKa = 9.01VLLTYY15 pKa = 10.06AQSGDD20 pKa = 3.41LSEE23 pKa = 4.47WDD25 pKa = 3.46ILDD28 pKa = 4.84HH29 pKa = 6.69ISSLGAEE36 pKa = 4.45CIIGRR41 pKa = 11.84EE42 pKa = 4.05DD43 pKa = 3.35HH44 pKa = 7.18ADD46 pKa = 3.64GGTHH50 pKa = 6.1LHH52 pKa = 5.87VFVDD56 pKa = 4.7FGRR59 pKa = 11.84KK60 pKa = 7.88KK61 pKa = 9.87QSRR64 pKa = 11.84RR65 pKa = 11.84SDD67 pKa = 3.46FFDD70 pKa = 3.73VGGHH74 pKa = 6.38HH75 pKa = 7.21PNITPSRR82 pKa = 11.84GRR84 pKa = 11.84PEE86 pKa = 4.18CGYY89 pKa = 11.01DD90 pKa = 3.43YY91 pKa = 10.75AIKK94 pKa = 10.91DD95 pKa = 3.59GDD97 pKa = 4.02VVAGGLARR105 pKa = 11.84PGGGGLPSLADD116 pKa = 3.18KK117 pKa = 10.37WRR119 pKa = 11.84TIVSAEE125 pKa = 3.84SRR127 pKa = 11.84EE128 pKa = 4.04EE129 pKa = 4.15FFDD132 pKa = 4.66LLRR135 pKa = 11.84EE136 pKa = 4.4LDD138 pKa = 3.72PKK140 pKa = 10.54TLVTRR145 pKa = 11.84WSEE148 pKa = 3.74LCRR151 pKa = 11.84YY152 pKa = 9.36ADD154 pKa = 3.21HH155 pKa = 7.31AYY157 pKa = 9.89EE158 pKa = 4.74SKK160 pKa = 10.38PEE162 pKa = 4.2PYY164 pKa = 9.93VGPSGVEE171 pKa = 3.94FEE173 pKa = 4.87LGMVPEE179 pKa = 4.41LVRR182 pKa = 11.84WRR184 pKa = 11.84GDD186 pKa = 3.18TLADD190 pKa = 4.5DD191 pKa = 4.8SEE193 pKa = 5.17LEE195 pKa = 4.18AWAHH199 pKa = 5.65TCIQWEE205 pKa = 4.32SCRR208 pKa = 11.84DD209 pKa = 3.56MFCCGTCRR217 pKa = 11.84EE218 pKa = 3.94VNYY221 pKa = 10.94AVFDD225 pKa = 4.14DD226 pKa = 3.83MRR228 pKa = 11.84GGIGMFPSFKK238 pKa = 10.13EE239 pKa = 3.77WLGAQAVVSVKK250 pKa = 10.32KK251 pKa = 10.67LYY253 pKa = 10.08RR254 pKa = 11.84DD255 pKa = 3.58PVQVPWGRR263 pKa = 11.84PCIWLSNADD272 pKa = 3.65PRR274 pKa = 11.84DD275 pKa = 3.68QIKK278 pKa = 10.81SGLSDD283 pKa = 3.26RR284 pKa = 11.84SSRR287 pKa = 11.84GQIEE291 pKa = 4.58LVEE294 pKa = 4.79NDD296 pKa = 3.65IAWLEE301 pKa = 4.24DD302 pKa = 2.93NCIFVEE308 pKa = 4.05LLEE311 pKa = 4.97PIFRR315 pKa = 11.84ASIGG319 pKa = 3.48

Molecular weight: 35.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4G0X9|A0A0M4G0X9_9VIRU Capsid protein OS=Poaceae-associated gemycircularvirus 1 OX=1708497 PE=4 SV=1
MM1 pKa = 7.78AYY3 pKa = 10.13ARR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 7.28STSYY11 pKa = 10.51RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.29PIRR18 pKa = 11.84RR19 pKa = 11.84SVRR22 pKa = 11.84RR23 pKa = 11.84SRR25 pKa = 11.84STVKK29 pKa = 8.65TRR31 pKa = 11.84RR32 pKa = 11.84YY33 pKa = 8.66KK34 pKa = 10.39RR35 pKa = 11.84IYY37 pKa = 8.97RR38 pKa = 11.84RR39 pKa = 11.84PMTKK43 pKa = 9.71RR44 pKa = 11.84RR45 pKa = 11.84ILNATSRR52 pKa = 11.84KK53 pKa = 9.73KK54 pKa = 10.46RR55 pKa = 11.84DD56 pKa = 3.23TMLAASNTNSAGSPLPNTGTRR77 pKa = 11.84AIVYY81 pKa = 9.61GGTSPPTGSSYY92 pKa = 11.61NGGFFLWCATARR104 pKa = 11.84DD105 pKa = 3.93LTINSGPAGAVAQEE119 pKa = 4.18ATRR122 pKa = 11.84TATTCYY128 pKa = 9.73MRR130 pKa = 11.84GLSEE134 pKa = 4.24KK135 pKa = 10.68LRR137 pKa = 11.84VQTNSPAPWFWRR149 pKa = 11.84RR150 pKa = 11.84ICFTIQAAFGVAAPGDD166 pKa = 4.49SPTNTYY172 pKa = 10.9SDD174 pKa = 3.76YY175 pKa = 11.57LEE177 pKa = 4.44NSNGFTRR184 pKa = 11.84LWLNQNINSQGNTTAGITNIVFKK207 pKa = 10.88GAVGRR212 pKa = 11.84DD213 pKa = 2.98WRR215 pKa = 11.84DD216 pKa = 3.43LISAPVDD223 pKa = 3.34TRR225 pKa = 11.84RR226 pKa = 11.84VNLKK230 pKa = 9.49YY231 pKa = 10.82DD232 pKa = 3.19KK233 pKa = 10.87TRR235 pKa = 11.84VFRR238 pKa = 11.84SGNQSGIVRR247 pKa = 11.84EE248 pKa = 4.14LKK250 pKa = 9.94LWHH253 pKa = 6.5PMNRR257 pKa = 11.84NLVYY261 pKa = 10.84DD262 pKa = 4.47DD263 pKa = 5.83DD264 pKa = 4.32EE265 pKa = 4.74VADD268 pKa = 3.87VEE270 pKa = 4.49NTSVYY275 pKa = 10.9SVVDD279 pKa = 3.24KK280 pKa = 11.19RR281 pKa = 11.84GMGNYY286 pKa = 9.16YY287 pKa = 10.43VLDD290 pKa = 4.74IIQPGEE296 pKa = 3.81ASSASDD302 pKa = 4.07LLLIDD307 pKa = 4.31STSSLYY313 pKa = 9.29WHH315 pKa = 7.07EE316 pKa = 4.24KK317 pKa = 9.23

Molecular weight: 35.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

833

197

319

277.7

31.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.963 ± 0.539

1.921 ± 0.606

7.323 ± 0.981

5.642 ± 1.37

3.842 ± 0.588

8.884 ± 0.854

2.281 ± 0.715

4.802 ± 0.277

3.601 ± 0.355

7.683 ± 0.594

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.681 ± 0.092

3.241 ± 1.457

4.802 ± 0.167

1.921 ± 0.253

8.884 ± 0.955

8.403 ± 0.511

5.642 ± 1.601

6.242 ± 0.167

2.521 ± 0.297

3.721 ± 0.503
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski