Gossypium punctatum mild leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; unclassified Begomovirus

Average proteome isoelectric point is 7.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|B8Q6T1|B8Q6T1_9GEMI AC4 OS=Gossypium punctatum mild leaf curl virus OX=509532 GN=AC4 PE=3 SV=1
MM1 pKa = 7.53PRR3 pKa = 11.84NSSFCVNAKK12 pKa = 10.45NIFLTYY18 pKa = 8.78PKK20 pKa = 10.44CPIPKK25 pKa = 9.74EE26 pKa = 3.91QMLDD30 pKa = 3.12NLRR33 pKa = 11.84SINCPSDD40 pKa = 3.17KK41 pKa = 10.94LFIRR45 pKa = 11.84VAQEE49 pKa = 3.46KK50 pKa = 9.49HH51 pKa = 5.19QDD53 pKa = 3.38GSLHH57 pKa = 5.16IHH59 pKa = 6.91ALIQFKK65 pKa = 10.83GKK67 pKa = 10.55AKK69 pKa = 9.97FRR71 pKa = 11.84NPRR74 pKa = 11.84HH75 pKa = 6.16FDD77 pKa = 3.03VTHH80 pKa = 7.16PNNSTQFHH88 pKa = 7.21PNFQGAKK95 pKa = 9.08SSSDD99 pKa = 3.06VKK101 pKa = 11.23SYY103 pKa = 10.87IEE105 pKa = 5.17KK106 pKa = 10.69DD107 pKa = 2.95DD108 pKa = 6.01DD109 pKa = 4.79YY110 pKa = 11.51IDD112 pKa = 3.45WGQFQVDD119 pKa = 3.43GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.91AAAEE136 pKa = 3.92ALNAGSAEE144 pKa = 3.87AALAIIRR151 pKa = 11.84EE152 pKa = 4.43KK153 pKa = 10.96LPKK156 pKa = 10.19DD157 pKa = 3.96YY158 pKa = 10.79IFQYY162 pKa = 11.21HH163 pKa = 5.76NLKK166 pKa = 10.79CNLDD170 pKa = 4.1RR171 pKa = 11.84IFTPPVEE178 pKa = 4.62VYY180 pKa = 10.26VSPFSPSSFDD190 pKa = 3.4QVPEE194 pKa = 3.95EE195 pKa = 4.36LEE197 pKa = 3.76VWAIDD202 pKa = 4.05NVVDD206 pKa = 4.08PAARR210 pKa = 11.84PLRR213 pKa = 11.84PRR215 pKa = 11.84SIVIEE220 pKa = 4.26GDD222 pKa = 3.21SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 4.79DD262 pKa = 4.33VDD264 pKa = 3.73PHH266 pKa = 6.08FLKK269 pKa = 10.67HH270 pKa = 5.9FKK272 pKa = 10.69EE273 pKa = 4.43FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.6YY287 pKa = 10.57GKK289 pKa = 9.12PVQIKK294 pKa = 10.41GGIPTIFLCNPGPTGSYY311 pKa = 10.52KK312 pKa = 10.65EE313 pKa = 4.13FLDD316 pKa = 3.96EE317 pKa = 4.83EE318 pKa = 4.8KK319 pKa = 10.25NTALKK324 pKa = 10.38NWAVKK329 pKa = 10.17NAIFITLEE337 pKa = 4.07GPLYY341 pKa = 10.88SGTNQSTAQGSEE353 pKa = 3.87EE354 pKa = 3.99THH356 pKa = 5.98QEE358 pKa = 3.91EE359 pKa = 4.9EE360 pKa = 4.36SRR362 pKa = 11.84SS363 pKa = 3.67

Molecular weight:
40.98 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|B8Q6S8|B8Q6S8_9GEMI Replication enhancer OS=Gossypium punctatum mild leaf curl virus OX=509532 GN=AC3 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 10.21RR4 pKa = 11.84AADD7 pKa = 3.55IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFGSPYY27 pKa = 8.67TSRR30 pKa = 11.84AAAPIVRR37 pKa = 11.84VTKK40 pKa = 9.91QQAWTNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.8PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.57RR60 pKa = 11.84SPDD63 pKa = 3.29VPKK66 pKa = 10.81GCEE69 pKa = 4.39GPCKK73 pKa = 10.05VQSFEE78 pKa = 4.02SRR80 pKa = 11.84HH81 pKa = 5.54DD82 pKa = 3.51VVHH85 pKa = 6.75IGKK88 pKa = 9.41VMCISDD94 pKa = 3.53VTRR97 pKa = 11.84GVGLTHH103 pKa = 7.8RR104 pKa = 11.84IGKK107 pKa = 8.54RR108 pKa = 11.84FCVKK112 pKa = 9.96SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.57NSHH172 pKa = 6.59RR173 pKa = 11.84DD174 pKa = 3.15RR175 pKa = 11.84YY176 pKa = 9.17QVLRR180 pKa = 11.84KK181 pKa = 8.43WHH183 pKa = 5.58ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.68KK201 pKa = 9.39FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.48FYY251 pKa = 11.32DD252 pKa = 3.71SVTHH256 pKa = 6.75

Molecular weight:
29.6 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1642

85

363

205.3

23.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.568 ± 0.753

2.375 ± 0.456

5.359 ± 0.423

4.324 ± 0.549

4.141 ± 0.395

5.116 ± 0.469

3.167 ± 0.554

5.725 ± 0.574

5.725 ± 0.553

6.638 ± 0.615

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.314 ± 0.467

5.725 ± 0.45

5.664 ± 0.451

4.75 ± 0.515

7.004 ± 0.758

9.318 ± 1.103

5.664 ± 0.62

6.638 ± 0.748

1.157 ± 0.13

4.629 ± 0.601

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski