Gossypium punctatum mild leaf curl virus
Average proteome isoelectric point is 7.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B8Q6T1|B8Q6T1_9GEMI AC4 OS=Gossypium punctatum mild leaf curl virus OX=509532 GN=AC4 PE=3 SV=1
MM1 pKa = 7.53 PRR3 pKa = 11.84 NSSFCVNAKK12 pKa = 10.45 NIFLTYY18 pKa = 8.78 PKK20 pKa = 10.44 CPIPKK25 pKa = 9.74 EE26 pKa = 3.91 QMLDD30 pKa = 3.12 NLRR33 pKa = 11.84 SINCPSDD40 pKa = 3.17 KK41 pKa = 10.94 LFIRR45 pKa = 11.84 VAQEE49 pKa = 3.46 KK50 pKa = 9.49 HH51 pKa = 5.19 QDD53 pKa = 3.38 GSLHH57 pKa = 5.16 IHH59 pKa = 6.91 ALIQFKK65 pKa = 10.83 GKK67 pKa = 10.55 AKK69 pKa = 9.97 FRR71 pKa = 11.84 NPRR74 pKa = 11.84 HH75 pKa = 6.16 FDD77 pKa = 3.03 VTHH80 pKa = 7.16 PNNSTQFHH88 pKa = 7.21 PNFQGAKK95 pKa = 9.08 SSSDD99 pKa = 3.06 VKK101 pKa = 11.23 SYY103 pKa = 10.87 IEE105 pKa = 5.17 KK106 pKa = 10.69 DD107 pKa = 2.95 DD108 pKa = 6.01 DD109 pKa = 4.79 YY110 pKa = 11.51 IDD112 pKa = 3.45 WGQFQVDD119 pKa = 3.43 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQTANDD132 pKa = 3.91 AAAEE136 pKa = 3.92 ALNAGSAEE144 pKa = 3.87 AALAIIRR151 pKa = 11.84 EE152 pKa = 4.43 KK153 pKa = 10.96 LPKK156 pKa = 10.19 DD157 pKa = 3.96 YY158 pKa = 10.79 IFQYY162 pKa = 11.21 HH163 pKa = 5.76 NLKK166 pKa = 10.79 CNLDD170 pKa = 4.1 RR171 pKa = 11.84 IFTPPVEE178 pKa = 4.62 VYY180 pKa = 10.26 VSPFSPSSFDD190 pKa = 3.4 QVPEE194 pKa = 3.95 EE195 pKa = 4.36 LEE197 pKa = 3.76 VWAIDD202 pKa = 4.05 NVVDD206 pKa = 4.08 PAARR210 pKa = 11.84 PLRR213 pKa = 11.84 PRR215 pKa = 11.84 SIVIEE220 pKa = 4.26 GDD222 pKa = 3.21 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.15 VYY251 pKa = 11.07 SNDD254 pKa = 2.25 AWYY257 pKa = 10.92 NVIDD261 pKa = 4.79 DD262 pKa = 4.33 VDD264 pKa = 3.73 PHH266 pKa = 6.08 FLKK269 pKa = 10.67 HH270 pKa = 5.9 FKK272 pKa = 10.69 EE273 pKa = 4.43 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.6 YY287 pKa = 10.57 GKK289 pKa = 9.12 PVQIKK294 pKa = 10.41 GGIPTIFLCNPGPTGSYY311 pKa = 10.52 KK312 pKa = 10.65 EE313 pKa = 4.13 FLDD316 pKa = 3.96 EE317 pKa = 4.83 EE318 pKa = 4.8 KK319 pKa = 10.25 NTALKK324 pKa = 10.38 NWAVKK329 pKa = 10.17 NAIFITLEE337 pKa = 4.07 GPLYY341 pKa = 10.88 SGTNQSTAQGSEE353 pKa = 3.87 EE354 pKa = 3.99 THH356 pKa = 5.98 QEE358 pKa = 3.91 EE359 pKa = 4.9 EE360 pKa = 4.36 SRR362 pKa = 11.84 SS363 pKa = 3.67
Molecular weight: 40.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.005
IPC2_protein 5.931
IPC_protein 6.02
Toseland 6.415
ProMoST 6.389
Dawson 6.338
Bjellqvist 6.313
Wikipedia 6.351
Rodwell 6.326
Grimsley 6.576
Solomon 6.338
Lehninger 6.338
Nozaki 6.605
DTASelect 6.795
Thurlkill 6.825
EMBOSS 6.795
Sillero 6.722
Patrickios 4.24
IPC_peptide 6.351
IPC2_peptide 6.649
IPC2.peptide.svr19 6.631
Protein with the highest isoelectric point:
>tr|B8Q6S8|B8Q6S8_9GEMI Replication enhancer OS=Gossypium punctatum mild leaf curl virus OX=509532 GN=AC3 PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 10.21 RR4 pKa = 11.84 AADD7 pKa = 3.55 IVISTPASKK16 pKa = 10.29 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFGSPYY27 pKa = 8.67 TSRR30 pKa = 11.84 AAAPIVRR37 pKa = 11.84 VTKK40 pKa = 9.91 QQAWTNRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.8 PRR54 pKa = 11.84 MYY56 pKa = 10.68 RR57 pKa = 11.84 MYY59 pKa = 10.57 RR60 pKa = 11.84 SPDD63 pKa = 3.29 VPKK66 pKa = 10.81 GCEE69 pKa = 4.39 GPCKK73 pKa = 10.05 VQSFEE78 pKa = 4.02 SRR80 pKa = 11.84 HH81 pKa = 5.54 DD82 pKa = 3.51 VVHH85 pKa = 6.75 IGKK88 pKa = 9.41 VMCISDD94 pKa = 3.53 VTRR97 pKa = 11.84 GVGLTHH103 pKa = 7.8 RR104 pKa = 11.84 IGKK107 pKa = 8.54 RR108 pKa = 11.84 FCVKK112 pKa = 9.96 SVYY115 pKa = 10.52 VLGKK119 pKa = 9.41 IWMDD123 pKa = 3.35 EE124 pKa = 4.04 NIKK127 pKa = 9.32 TKK129 pKa = 10.6 NHH131 pKa = 5.78 TNSVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.84 RR143 pKa = 11.84 RR144 pKa = 11.84 PVDD147 pKa = 3.13 KK148 pKa = 10.28 PQDD151 pKa = 3.56 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.57 NSHH172 pKa = 6.59 RR173 pKa = 11.84 DD174 pKa = 3.15 RR175 pKa = 11.84 YY176 pKa = 9.17 QVLRR180 pKa = 11.84 KK181 pKa = 8.43 WHH183 pKa = 5.58 ATVTGGQYY191 pKa = 10.85 ASKK194 pKa = 10.24 EE195 pKa = 3.73 QALVKK200 pKa = 10.68 KK201 pKa = 9.39 FVRR204 pKa = 11.84 VNNYY208 pKa = 7.82 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.1 YY219 pKa = 8.23 EE220 pKa = 4.02 NHH222 pKa = 6.47 TEE224 pKa = 3.99 NALMLYY230 pKa = 7.52 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.48 FYY251 pKa = 11.32 DD252 pKa = 3.71 SVTHH256 pKa = 6.75
Molecular weight: 29.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.251
IPC2_protein 9.531
IPC_protein 9.692
Toseland 10.145
ProMoST 9.853
Dawson 10.35
Bjellqvist 10.043
Wikipedia 10.526
Rodwell 10.73
Grimsley 10.423
Solomon 10.379
Lehninger 10.335
Nozaki 10.16
DTASelect 10.028
Thurlkill 10.204
EMBOSS 10.555
Sillero 10.262
Patrickios 10.306
IPC_peptide 10.365
IPC2_peptide 8.858
IPC2.peptide.svr19 8.472
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1642
85
363
205.3
23.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.568 ± 0.753
2.375 ± 0.456
5.359 ± 0.423
4.324 ± 0.549
4.141 ± 0.395
5.116 ± 0.469
3.167 ± 0.554
5.725 ± 0.574
5.725 ± 0.553
6.638 ± 0.615
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.314 ± 0.467
5.725 ± 0.45
5.664 ± 0.451
4.75 ± 0.515
7.004 ± 0.758
9.318 ± 1.103
5.664 ± 0.62
6.638 ± 0.748
1.157 ± 0.13
4.629 ± 0.601
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here