Aeromonas phage 32

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A219YBZ7|A0A219YBZ7_9CAUD Uncharacterized protein OS=Aeromonas phage 32 OX=1932899 PE=4 SV=1
MM1 pKa = 7.74KK2 pKa = 10.13DD3 pKa = 3.08VTVIAGVAVAPGQVVCGVKK22 pKa = 10.36GPSIITPEE30 pKa = 3.89VPTFGEE36 pKa = 4.64YY37 pKa = 9.3WSHH40 pKa = 5.31TAMNGLPEE48 pKa = 4.22MTWDD52 pKa = 3.51AFQARR57 pKa = 11.84MMNQYY62 pKa = 10.91GLTILYY68 pKa = 10.34RR69 pKa = 11.84SMQDD73 pKa = 3.26DD74 pKa = 4.46PEE76 pKa = 4.37VDD78 pKa = 3.16WEE80 pKa = 4.5AEE82 pKa = 4.14LEE84 pKa = 4.1LSEE87 pKa = 4.97WKK89 pKa = 9.27PQNPFISDD97 pKa = 3.4KK98 pKa = 11.45AFLLVLGEE106 pKa = 4.81DD107 pKa = 3.67EE108 pKa = 5.43DD109 pKa = 5.34GPFAVWGLPQQ119 pKa = 4.03

Molecular weight: 13.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A219YBW5|A0A219YBW5_9CAUD Uncharacterized protein OS=Aeromonas phage 32 OX=1932899 PE=4 SV=1
MM1 pKa = 6.71TKK3 pKa = 9.97KK4 pKa = 10.48VKK6 pKa = 10.48RR7 pKa = 11.84NLFQMIDD14 pKa = 3.13RR15 pKa = 11.84SFSRR19 pKa = 11.84VTGRR23 pKa = 11.84EE24 pKa = 3.32HH25 pKa = 6.22QYY27 pKa = 10.66CVKK30 pKa = 10.78LSFKK34 pKa = 10.16PKK36 pKa = 8.45GWAGQPHH43 pKa = 6.03TWVVLPGMTYY53 pKa = 10.18RR54 pKa = 11.84PHH56 pKa = 6.88SQEE59 pKa = 3.91FYY61 pKa = 9.92RR62 pKa = 11.84SLRR65 pKa = 11.84KK66 pKa = 9.74HH67 pKa = 5.29YY68 pKa = 10.52GEE70 pKa = 4.54RR71 pKa = 11.84FINGLRR77 pKa = 11.84KK78 pKa = 10.04LGINPNNGQITIDD91 pKa = 3.61SITYY95 pKa = 10.25LGRR98 pKa = 11.84HH99 pKa = 5.32

Molecular weight: 11.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

14409

42

868

173.6

19.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.653 ± 0.516

1.534 ± 0.186

5.316 ± 0.208

6.35 ± 0.327

3.04 ± 0.131

7.613 ± 0.38

2.401 ± 0.178

4.941 ± 0.198

6.156 ± 0.265

8.509 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.317 ± 0.151

3.421 ± 0.217

4.351 ± 0.267

3.727 ± 0.191

5.962 ± 0.31

5.566 ± 0.213

6.329 ± 0.245

6.878 ± 0.275

1.631 ± 0.107

2.304 ± 0.136
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski