Apis mellifera associated microvirus 40
Average proteome isoelectric point is 7.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3S8UU45|A0A3S8UU45_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 40 OX=2494770 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 FDD4 pKa = 3.17 EE5 pKa = 5.12 HH6 pKa = 8.13 GRR8 pKa = 11.84 EE9 pKa = 4.34 LPDD12 pKa = 3.68 DD13 pKa = 3.89 TPIAKK18 pKa = 9.32 PVRR21 pKa = 11.84 FNRR24 pKa = 11.84 PGSTLDD30 pKa = 3.51 EE31 pKa = 4.08 IRR33 pKa = 11.84 RR34 pKa = 11.84 NLDD37 pKa = 2.84 MANRR41 pKa = 11.84 LAQEE45 pKa = 4.04 QGLEE49 pKa = 4.27 TFDD52 pKa = 4.26 EE53 pKa = 4.92 ADD55 pKa = 4.05 DD56 pKa = 3.97 FQIGDD61 pKa = 4.18 DD62 pKa = 4.14 FEE64 pKa = 6.16 EE65 pKa = 3.93 NHH67 pKa = 6.36 PWAEE71 pKa = 4.41 QIDD74 pKa = 3.9 NMNRR78 pKa = 11.84 EE79 pKa = 4.15 FEE81 pKa = 4.35 EE82 pKa = 3.52 IRR84 pKa = 11.84 NKK86 pKa = 9.78 VRR88 pKa = 11.84 EE89 pKa = 3.86 ARR91 pKa = 11.84 LRR93 pKa = 11.84 GEE95 pKa = 4.07 IEE97 pKa = 3.93 VTEE100 pKa = 4.43 DD101 pKa = 3.12 GRR103 pKa = 11.84 WYY105 pKa = 10.51 SKK107 pKa = 10.66 PKK109 pKa = 9.91 AAEE112 pKa = 3.81 PSRR115 pKa = 11.84 GVPEE119 pKa = 4.47 APQPSVSNAQLSQPANGAPPNQPSPSAPNNPNKK152 pKa = 10.23 PP153 pKa = 3.47
Molecular weight: 17.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.823
IPC2_protein 4.685
IPC_protein 4.584
Toseland 4.444
ProMoST 4.685
Dawson 4.52
Bjellqvist 4.673
Wikipedia 4.38
Rodwell 4.431
Grimsley 4.355
Solomon 4.52
Lehninger 4.482
Nozaki 4.635
DTASelect 4.762
Thurlkill 4.431
EMBOSS 4.406
Sillero 4.698
Patrickios 4.406
IPC_peptide 4.533
IPC2_peptide 4.685
IPC2.peptide.svr19 4.654
Protein with the highest isoelectric point:
>tr|A0A3S8UU45|A0A3S8UU45_9VIRU Uncharacterized protein OS=Apis mellifera associated microvirus 40 OX=2494770 PE=4 SV=1
MM1 pKa = 7.72 RR2 pKa = 11.84 FSGLPARR9 pKa = 11.84 VVAAPLKK16 pKa = 9.19 KK17 pKa = 8.89 TATARR22 pKa = 11.84 SKK24 pKa = 10.76 GLRR27 pKa = 11.84 RR28 pKa = 11.84 TSMGALLFSAPSRR41 pKa = 11.84 VAVCVQRR48 pKa = 11.84 GVRR51 pKa = 11.84 KK52 pKa = 9.14 EE53 pKa = 3.89 VLHH56 pKa = 5.58 ATGNVGRR63 pKa = 11.84 NRR65 pKa = 11.84 PGRR68 pKa = 11.84 PGPFSKK74 pKa = 10.54 VRR76 pKa = 11.84 CKK78 pKa = 10.77
Molecular weight: 8.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.269
IPC2_protein 10.57
IPC_protein 12.135
Toseland 12.31
ProMoST 12.808
Dawson 12.31
Bjellqvist 12.31
Wikipedia 12.793
Rodwell 12.047
Grimsley 12.354
Solomon 12.808
Lehninger 12.705
Nozaki 12.31
DTASelect 12.31
Thurlkill 12.31
EMBOSS 12.808
Sillero 12.31
Patrickios 11.769
IPC_peptide 12.808
IPC2_peptide 11.798
IPC2.peptide.svr19 9.188
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1351
78
493
270.2
29.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.215 ± 0.878
0.814 ± 0.455
4.589 ± 0.769
6.144 ± 0.994
3.183 ± 0.673
8.956 ± 0.588
2.369 ± 0.357
3.701 ± 0.513
3.553 ± 0.527
8.364 ± 0.918
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.147 ± 0.256
3.331 ± 0.878
6.662 ± 1.134
4.663 ± 0.307
8.808 ± 0.877
6.736 ± 1.157
5.477 ± 0.642
5.699 ± 0.618
1.628 ± 0.285
2.961 ± 0.604
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here