Pseudomonas phage vB_PaeS-Yazdi-M

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Septimatrevirus; unclassified Septimatrevirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S6MLM7|A0A6S6MLM7_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeS-Yazdi-M OX=2746928 PE=4 SV=1
MM1 pKa = 7.36GAYY4 pKa = 8.0TVTTEE9 pKa = 4.8FEE11 pKa = 4.38MNDD14 pKa = 3.18GRR16 pKa = 11.84ILSCEE21 pKa = 4.05YY22 pKa = 10.69GVSFTPGNYY31 pKa = 9.59SGLPEE36 pKa = 3.95NCYY39 pKa = 9.96PDD41 pKa = 3.68EE42 pKa = 5.41SEE44 pKa = 4.73AGEE47 pKa = 3.85PTYY50 pKa = 10.98YY51 pKa = 9.7IDD53 pKa = 5.4GEE55 pKa = 4.3EE56 pKa = 3.99VDD58 pKa = 5.38YY59 pKa = 11.6KK60 pKa = 11.02DD61 pKa = 5.12LPKK64 pKa = 11.02GLDD67 pKa = 3.34KK68 pKa = 10.99IADD71 pKa = 3.8KK72 pKa = 10.79LYY74 pKa = 9.4EE75 pKa = 4.62AGPGEE80 pKa = 4.26YY81 pKa = 9.83GYY83 pKa = 10.97SEE85 pKa = 4.61TEE87 pKa = 3.67PDD89 pKa = 3.47YY90 pKa = 11.36DD91 pKa = 4.2GPDD94 pKa = 3.71YY95 pKa = 11.4EE96 pKa = 5.96PDD98 pKa = 3.39DD99 pKa = 4.41YY100 pKa = 11.83YY101 pKa = 11.96

Molecular weight: 11.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S6MED1|A0A6S6MED1_9CAUD Uncharacterized protein OS=Pseudomonas phage vB_PaeS-Yazdi-M OX=2746928 PE=4 SV=1
MM1 pKa = 7.19YY2 pKa = 10.81ALILIACTVVAVAIHH17 pKa = 6.47FGLIRR22 pKa = 11.84PVMHH26 pKa = 6.99AIEE29 pKa = 4.23RR30 pKa = 11.84QRR32 pKa = 11.84EE33 pKa = 3.89EE34 pKa = 3.8SRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84AALEE42 pKa = 4.08AGLAAAARR50 pKa = 11.84LQAFNARR57 pKa = 11.84KK58 pKa = 9.01PSNHH62 pKa = 6.63RR63 pKa = 11.84GAKK66 pKa = 9.87LNAEE70 pKa = 4.74RR71 pKa = 11.84EE72 pKa = 4.47WQSQEE77 pKa = 3.5

Molecular weight: 8.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

12681

37

1275

230.6

25.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.576 ± 0.871

1.001 ± 0.168

5.197 ± 0.255

6.246 ± 0.306

3.896 ± 0.222

7.673 ± 0.331

1.27 ± 0.172

5.473 ± 0.197

5.544 ± 0.306

7.428 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.122

4.716 ± 0.241

5.165 ± 0.448

4.337 ± 0.691

5.149 ± 0.252

5.567 ± 0.313

6.017 ± 0.345

6.821 ± 0.237

1.404 ± 0.128

3.288 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski