Thioalkalivibrio nitratireducens (strain DSM 14787 / UNIQEM 213 / ALEN2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Thioalkalivibrio; Thioalkalivibrio nitratireducens

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3764 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0DQJ7|L0DQJ7_THIND PilT protein-like protein OS=Thioalkalivibrio nitratireducens (strain DSM 14787 / UNIQEM 213 / ALEN2) OX=1255043 GN=TVNIR_0024 PE=4 SV=1
MM1 pKa = 7.82AEE3 pKa = 4.12TMTASADD10 pKa = 3.49ADD12 pKa = 3.68FEE14 pKa = 4.61MPSPLIFTDD23 pKa = 3.89AAATKK28 pKa = 9.79VKK30 pKa = 10.76SLIDD34 pKa = 3.55EE35 pKa = 4.5EE36 pKa = 4.73EE37 pKa = 4.06NPNLKK42 pKa = 10.38LRR44 pKa = 11.84VFVSGGGCSGFQYY57 pKa = 10.95GFTFDD62 pKa = 3.61EE63 pKa = 4.74TVGDD67 pKa = 3.72GDD69 pKa = 3.94TVVEE73 pKa = 4.21NGGVTLLIDD82 pKa = 3.79PMSFQYY88 pKa = 10.95LAGAEE93 pKa = 3.77IDD95 pKa = 3.76YY96 pKa = 10.78TEE98 pKa = 4.44GLEE101 pKa = 4.19GAQFVIRR108 pKa = 11.84NPNATTTCGCGSSFSPP124 pKa = 4.57

Molecular weight: 13.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0E488|L0E488_THIND Uncharacterized protein OS=Thioalkalivibrio nitratireducens (strain DSM 14787 / UNIQEM 213 / ALEN2) OX=1255043 GN=TVNIR_3862 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LHH11 pKa = 6.59RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84SGRR28 pKa = 11.84RR29 pKa = 11.84VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.69GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LCPP44 pKa = 3.7

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3764

0

3764

1108119

37

2395

294.4

32.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.711 ± 0.062

0.939 ± 0.02

5.83 ± 0.034

6.364 ± 0.041

3.42 ± 0.028

8.483 ± 0.042

2.54 ± 0.022

4.312 ± 0.032

1.977 ± 0.03

11.168 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.201 ± 0.019

2.197 ± 0.02

5.695 ± 0.034

3.469 ± 0.025

8.995 ± 0.052

4.708 ± 0.031

4.777 ± 0.027

7.45 ± 0.039

1.521 ± 0.02

2.243 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski