Staphylococcus phage SA97

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Dubowvirus; Staphylococcus virus SA97

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F6N3K8|A0A0F6N3K8_9CAUD Capsid protein OS=Staphylococcus phage SA97 OX=1498171 GN=SA97_015 PE=4 SV=1
MM1 pKa = 6.89QQQAYY6 pKa = 9.38INATIDD12 pKa = 2.95IRR14 pKa = 11.84IPTEE18 pKa = 3.56VEE20 pKa = 3.88YY21 pKa = 11.22KK22 pKa = 10.82HH23 pKa = 6.75FGDD26 pKa = 4.47VDD28 pKa = 3.64NEE30 pKa = 3.66KK31 pKa = 10.93DD32 pKa = 3.32ALADD36 pKa = 3.51YY37 pKa = 10.65LYY39 pKa = 11.0NNPNEE44 pKa = 3.8ILEE47 pKa = 3.94YY48 pKa = 11.1DD49 pKa = 3.47NLKK52 pKa = 9.73IRR54 pKa = 11.84NVNIEE59 pKa = 3.84VEE61 pKa = 4.2

Molecular weight: 7.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F6N3E5|A0A0F6N3E5_9CAUD Transcriptional activator RinA OS=Staphylococcus phage SA97 OX=1498171 GN=SA97_009 PE=4 SV=1
MM1 pKa = 7.57SIVKK5 pKa = 10.22INGKK9 pKa = 8.42PYY11 pKa = 10.87KK12 pKa = 9.54FTEE15 pKa = 4.42HH16 pKa = 6.09EE17 pKa = 4.0NEE19 pKa = 4.33LIKK22 pKa = 11.15KK23 pKa = 9.98NGLTPGMVAKK33 pKa = 10.32RR34 pKa = 11.84VRR36 pKa = 11.84GGWALLEE43 pKa = 4.48ALHH46 pKa = 6.68APYY49 pKa = 11.09GMRR52 pKa = 11.84LAEE55 pKa = 4.12YY56 pKa = 10.62KK57 pKa = 10.65EE58 pKa = 4.08IVLSKK63 pKa = 10.44IMEE66 pKa = 4.38RR67 pKa = 11.84EE68 pKa = 4.03SKK70 pKa = 9.36EE71 pKa = 4.1RR72 pKa = 11.84EE73 pKa = 3.7MARR76 pKa = 11.84QRR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 8.97EE81 pKa = 3.76AEE83 pKa = 3.84LRR85 pKa = 11.84RR86 pKa = 11.84KK87 pKa = 9.75KK88 pKa = 10.2PHH90 pKa = 6.56LFNVPQVHH98 pKa = 5.79PRR100 pKa = 11.84GRR102 pKa = 11.84YY103 pKa = 8.85ACYY106 pKa = 10.52LMEE109 pKa = 5.36NDD111 pKa = 4.09IFVKK115 pKa = 10.41VKK117 pKa = 10.3KK118 pKa = 10.51

Molecular weight: 13.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12004

50

1155

222.3

25.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.84 ± 0.404

0.367 ± 0.068

6.348 ± 0.381

7.581 ± 0.601

4.29 ± 0.26

5.781 ± 0.249

1.666 ± 0.166

7.506 ± 0.341

9.272 ± 0.402

7.389 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.163

6.814 ± 0.303

2.691 ± 0.248

3.732 ± 0.198

4.124 ± 0.298

5.831 ± 0.325

6.498 ± 0.278

6.198 ± 0.311

1.208 ± 0.175

4.39 ± 0.358
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski