Chrysosporum ovalisporum
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2851 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A654S6I3|A0A654S6I3_9CYAN AAA ATPase central domain protein OS=Chrysosporum ovalisporum OX=75695 GN=apha_00368 PE=4 SV=1
MM1 pKa = 7.69 VLDD4 pKa = 4.43 SFNRR8 pKa = 11.84 WLIPLVVTTVSLYY21 pKa = 11.06 SNPAKK26 pKa = 10.64 ALAQTVYY33 pKa = 9.59 PFSGEE38 pKa = 4.11 YY39 pKa = 7.06 EE40 pKa = 4.02 TTIIIEE46 pKa = 4.67 PISDD50 pKa = 3.31 NLAQVSEE57 pKa = 4.39 RR58 pKa = 11.84 AVSDD62 pKa = 3.48 NAPYY66 pKa = 11.01 NLGLYY71 pKa = 9.8 EE72 pKa = 4.27 GLTYY76 pKa = 11.03 SFTDD80 pKa = 3.23 EE81 pKa = 4.27 NGNVAFNIDD90 pKa = 3.4 PTVFGMEE97 pKa = 5.29 GYY99 pKa = 9.05 PLGYY103 pKa = 9.77 ISFGNGANTIFGNADD118 pKa = 3.0 ANASIDD124 pKa = 4.3 FEE126 pKa = 4.36 NLTAKK131 pKa = 10.64 GSGILNITGGTGMFKK146 pKa = 10.65 NATGTLTFIQEE157 pKa = 4.22 DD158 pKa = 4.73 TVNLGPTTILNGVALVNGYY177 pKa = 8.11 ITVPQSVPEE186 pKa = 4.12 PTNITPLIGLALTGVGFFRR205 pKa = 11.84 YY206 pKa = 9.73 LFPNN210 pKa = 3.79
Molecular weight: 22.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.85
IPC2_protein 3.948
IPC_protein 3.859
Toseland 3.668
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.579
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.113
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.973
Patrickios 0.362
IPC_peptide 3.808
IPC2_peptide 3.948
IPC2.peptide.svr19 3.861
Protein with the highest isoelectric point:
>tr|A0A654SLM1|A0A654SLM1_9CYAN Thiosulphate-binding protein OS=Chrysosporum ovalisporum OX=75695 GN=apha_01072 PE=4 SV=1
MM1 pKa = 7.25 TIEE4 pKa = 4.39 RR5 pKa = 11.84 VPKK8 pKa = 9.43 VHH10 pKa = 6.36 YY11 pKa = 9.18 PKK13 pKa = 10.8 ADD15 pKa = 2.8 IGRR18 pKa = 11.84 RR19 pKa = 11.84 GMALGLDD26 pKa = 4.28 FLGVWLITCILGSSQIGIQLVQILIFICCWLILRR60 pKa = 11.84 VLVVYY65 pKa = 10.54 NNQGQSLGRR74 pKa = 11.84 WAFDD78 pKa = 3.57 LKK80 pKa = 10.56 ILEE83 pKa = 4.71 VIHH86 pKa = 7.0 GEE88 pKa = 3.58 ITGRR92 pKa = 11.84 IPTLRR97 pKa = 11.84 SLFKK101 pKa = 10.53 RR102 pKa = 11.84 EE103 pKa = 3.97 AIIGFGTLLVSIALSNIRR121 pKa = 11.84 ANPTAILLLLPLAIDD136 pKa = 4.37 CGAALSDD143 pKa = 4.5 KK144 pKa = 10.79 RR145 pKa = 11.84 MQQALHH151 pKa = 6.68 DD152 pKa = 4.63 RR153 pKa = 11.84 YY154 pKa = 10.65 AGTFIVSSRR163 pKa = 11.84 RR164 pKa = 11.84 GYY166 pKa = 11.0 SLDD169 pKa = 3.27 IKK171 pKa = 10.46 IKK173 pKa = 10.39 RR174 pKa = 11.84 LLEE177 pKa = 3.68 KK178 pKa = 9.31 MRR180 pKa = 11.84 RR181 pKa = 11.84 SVRR184 pKa = 11.84 RR185 pKa = 3.33
Molecular weight: 20.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.604
IPC_protein 10.335
Toseland 10.599
ProMoST 10.452
Dawson 10.701
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.935
Grimsley 10.76
Solomon 10.804
Lehninger 10.774
Nozaki 10.613
DTASelect 10.409
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.643
Patrickios 10.657
IPC_peptide 10.818
IPC2_peptide 9.56
IPC2.peptide.svr19 8.649
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2851
0
2851
968037
15
1795
339.5
37.83
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.013 ± 0.043
1.019 ± 0.013
4.637 ± 0.034
6.101 ± 0.04
3.899 ± 0.029
6.84 ± 0.04
2.076 ± 0.021
6.863 ± 0.037
4.773 ± 0.037
10.996 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.967 ± 0.018
4.35 ± 0.027
4.869 ± 0.03
5.431 ± 0.038
5.051 ± 0.034
6.182 ± 0.031
5.675 ± 0.03
6.803 ± 0.04
1.434 ± 0.023
3.02 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here