Streptococcus phage Javan178

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AS32|A0A4D6AS32_9CAUD Uncharacterized protein OS=Streptococcus phage Javan178 OX=2548025 GN=Javan178_0006 PE=4 SV=1
MM1 pKa = 7.47NKK3 pKa = 9.3EE4 pKa = 3.89QLIKK8 pKa = 9.79TIKK11 pKa = 7.85EE12 pKa = 3.63TDD14 pKa = 3.35YY15 pKa = 11.35NFYY18 pKa = 10.92AVRR21 pKa = 11.84GWDD24 pKa = 3.15EE25 pKa = 4.31GYY27 pKa = 10.73KK28 pKa = 9.88IGEE31 pKa = 4.22VLPEE35 pKa = 4.11SVKK38 pKa = 9.88WDD40 pKa = 3.68YY41 pKa = 11.29EE42 pKa = 4.11RR43 pKa = 11.84DD44 pKa = 3.5MPTDD48 pKa = 3.2IEE50 pKa = 4.43VGGTCTTIPDD60 pKa = 3.95SQHH63 pKa = 7.11SDD65 pKa = 2.99FKK67 pKa = 11.19DD68 pKa = 3.65YY69 pKa = 11.75YY70 pKa = 8.21EE71 pKa = 4.58TEE73 pKa = 4.02EE74 pKa = 4.05EE75 pKa = 4.01MIEE78 pKa = 4.33AIEE81 pKa = 4.17KK82 pKa = 9.76AVKK85 pKa = 9.77INKK88 pKa = 9.44SNYY91 pKa = 9.14CYY93 pKa = 10.87SKK95 pKa = 10.8LYY97 pKa = 10.9LVGSYY102 pKa = 10.19NRR104 pKa = 11.84NPYY107 pKa = 8.92NAYY110 pKa = 9.82EE111 pKa = 4.14ADD113 pKa = 3.75EE114 pKa = 4.64NEE116 pKa = 5.2AILADD121 pKa = 3.57AVVLMEE127 pKa = 4.57LL128 pKa = 4.06

Molecular weight: 14.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6ATE0|A0A4D6ATE0_9CAUD Hyaluronidase OS=Streptococcus phage Javan178 OX=2548025 GN=Javan178_0049 PE=4 SV=1
MM1 pKa = 7.41AKK3 pKa = 9.65IVVEE7 pKa = 3.79LGGIKK12 pKa = 10.21RR13 pKa = 11.84KK14 pKa = 10.05VSPQALAKK22 pKa = 10.41GKK24 pKa = 10.36LVMNNQVMITMNPYY38 pKa = 9.17VPYY41 pKa = 10.04RR42 pKa = 11.84DD43 pKa = 3.26GSLRR47 pKa = 11.84GSSRR51 pKa = 11.84ADD53 pKa = 3.34SIGVTWSGPHH63 pKa = 6.66ARR65 pKa = 11.84PQFYY69 pKa = 10.38GGAYY73 pKa = 9.91NKK75 pKa = 10.37YY76 pKa = 10.46KK77 pKa = 10.58SFKK80 pKa = 8.64FKK82 pKa = 10.8KK83 pKa = 8.45YY84 pKa = 5.33TTPGTGKK91 pKa = 10.19RR92 pKa = 11.84WDD94 pKa = 3.49KK95 pKa = 10.88RR96 pKa = 11.84ALANATIVKK105 pKa = 9.99DD106 pKa = 3.47WEE108 pKa = 4.06QSLLRR113 pKa = 11.84GMGFRR118 pKa = 4.66

Molecular weight: 13.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

11901

42

1086

201.7

22.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.604 ± 0.72

0.714 ± 0.11

6.941 ± 0.368

7.31 ± 0.347

3.84 ± 0.222

6.218 ± 0.302

1.504 ± 0.146

7.025 ± 0.289

8.856 ± 0.34

8.134 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.201 ± 0.152

5.277 ± 0.2

2.748 ± 0.246

4.21 ± 0.259

4.487 ± 0.281

5.806 ± 0.365

5.731 ± 0.207

6.1 ± 0.247

1.202 ± 0.121

4.092 ± 0.336
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski