Brachypodium distachyon (Purple false brome) (Trachynia distachya)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Pooideae; Stipodae; Brachypodieae; Brachypodium

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44786 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1HEN4|I1HEN4_BRADI Isoform of A0A0Q3IDK8 RanBP2-type domain-containing protein OS=Brachypodium distachyon OX=15368 GN=100836020 PE=3 SV=1
MM1 pKa = 8.05SGMACYY7 pKa = 10.49LLPEE11 pKa = 4.93GGNIFPSDD19 pKa = 3.51EE20 pKa = 4.01EE21 pKa = 4.39LFEE24 pKa = 4.24NQNAEE29 pKa = 4.02QGDD32 pKa = 4.61YY33 pKa = 6.85WTQWNSRR40 pKa = 11.84LSDD43 pKa = 3.96DD44 pKa = 5.23LNTSSIYY51 pKa = 10.11SDD53 pKa = 2.85KK54 pKa = 11.18HH55 pKa = 6.36EE56 pKa = 5.0EE57 pKa = 4.26GSAQCFDD64 pKa = 3.9ADD66 pKa = 3.29EE67 pKa = 4.56HH68 pKa = 6.3QRR70 pKa = 11.84SNQCEE75 pKa = 3.87RR76 pKa = 11.84SSEE79 pKa = 4.06CDD81 pKa = 2.83RR82 pKa = 11.84AASSGCSTGQSEE94 pKa = 4.64EE95 pKa = 4.07QSKK98 pKa = 10.99GPAPLEE104 pKa = 3.84LQQTKK109 pKa = 7.57EE110 pKa = 4.2TNDD113 pKa = 2.9IFLSQFSDD121 pKa = 3.56DD122 pKa = 4.33EE123 pKa = 4.24MRR125 pKa = 11.84MMDD128 pKa = 4.06TPFQALDD135 pKa = 3.47MFPGSMHH142 pKa = 7.23RR143 pKa = 11.84LLSYY147 pKa = 11.23EE148 pKa = 3.94NMLSGVLTDD157 pKa = 3.84STNQVANQDD166 pKa = 3.76RR167 pKa = 11.84NEE169 pKa = 4.2MDD171 pKa = 3.26TMDD174 pKa = 3.44TCGFPLFSHH183 pKa = 7.56DD184 pKa = 5.09LQDD187 pKa = 4.89DD188 pKa = 3.83PSNADD193 pKa = 3.21GSIEE197 pKa = 4.15TLVNPSMDD205 pKa = 3.18KK206 pKa = 11.23SEE208 pKa = 4.0VSTMKK213 pKa = 10.52RR214 pKa = 11.84SWSTADD220 pKa = 3.84IEE222 pKa = 4.65SSSNGEE228 pKa = 3.91GAVLEE233 pKa = 4.16EE234 pKa = 4.68LEE236 pKa = 4.77DD237 pKa = 3.83VVFQVCSPEE246 pKa = 3.58EE247 pKa = 5.13GYY249 pKa = 10.99CFWEE253 pKa = 5.0VYY255 pKa = 10.03FIHH258 pKa = 5.94VTLHH262 pKa = 6.57LYY264 pKa = 9.88MSVSASEE271 pKa = 3.96ADD273 pKa = 4.61KK274 pKa = 11.31EE275 pKa = 4.47DD276 pKa = 3.42ACLPPP281 pKa = 5.54

Molecular weight: 31.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q3FWT4|A0A0Q3FWT4_BRADI Protein kinase domain-containing protein OS=Brachypodium distachyon OX=15368 GN=100837060 PE=3 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84VRR6 pKa = 11.84PAGARR11 pKa = 11.84TVRR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84LRR19 pKa = 11.84AGIRR23 pKa = 11.84TAGARR28 pKa = 11.84TVRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84PRR36 pKa = 11.84AAAGVRR42 pKa = 11.84RR43 pKa = 11.84GPRR46 pKa = 11.84AAAARR51 pKa = 11.84IRR53 pKa = 11.84WRR55 pKa = 11.84PWAAAAAARR64 pKa = 11.84RR65 pKa = 11.84LPSAWRR71 pKa = 11.84LPGAARR77 pKa = 11.84AGRR80 pKa = 11.84LPAGGLPHH88 pKa = 7.1EE89 pKa = 4.89LPRR92 pKa = 11.84AASARR97 pKa = 11.84LHH99 pKa = 6.55GIVPHH104 pKa = 6.0RR105 pKa = 11.84ARR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84RR110 pKa = 11.84RR111 pKa = 11.84PWRR114 pKa = 11.84EE115 pKa = 3.32AQGRR119 pKa = 11.84RR120 pKa = 11.84HH121 pKa = 4.52VRR123 pKa = 11.84RR124 pKa = 11.84RR125 pKa = 11.84QARR128 pKa = 11.84PQVEE132 pKa = 4.56VKK134 pKa = 10.69NRR136 pKa = 11.84IATRR140 pKa = 11.84PSVV143 pKa = 3.07

Molecular weight: 16.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

34230

10556

44786

18330814

23

5373

409.3

45.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.797 ± 0.016

1.988 ± 0.006

5.389 ± 0.008

6.046 ± 0.012

3.719 ± 0.006

7.127 ± 0.014

2.515 ± 0.005

4.453 ± 0.008

5.037 ± 0.01

9.614 ± 0.016

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.005

3.579 ± 0.008

5.583 ± 0.014

3.564 ± 0.009

6.275 ± 0.011

8.575 ± 0.014

4.816 ± 0.007

6.636 ± 0.009

1.299 ± 0.004

2.554 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski