Amino acid dipepetide frequency for Serratia sp. DD3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.443AlaAla: 9.443 ± 0.115
1.132AlaCys: 1.132 ± 0.027
4.848AlaAsp: 4.848 ± 0.058
5.746AlaGlu: 5.746 ± 0.076
3.412AlaPhe: 3.412 ± 0.053
7.271AlaGly: 7.271 ± 0.096
1.834AlaHis: 1.834 ± 0.039
5.904AlaIle: 5.904 ± 0.078
3.882AlaLys: 3.882 ± 0.063
11.27AlaLeu: 11.27 ± 0.113
2.707AlaMet: 2.707 ± 0.05
3.485AlaAsn: 3.485 ± 0.071
3.443AlaPro: 3.443 ± 0.058
4.561AlaGln: 4.561 ± 0.071
4.736AlaArg: 4.736 ± 0.072
5.134AlaSer: 5.134 ± 0.071
4.753AlaThr: 4.753 ± 0.071
6.39AlaVal: 6.39 ± 0.08
1.256AlaTrp: 1.256 ± 0.033
2.276AlaTyr: 2.276 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.939CysAla: 0.939 ± 0.027
0.194CysCys: 0.194 ± 0.013
0.596CysAsp: 0.596 ± 0.024
0.567CysGlu: 0.567 ± 0.021
0.468CysPhe: 0.468 ± 0.022
1.041CysGly: 1.041 ± 0.03
0.361CysHis: 0.361 ± 0.018
0.599CysIle: 0.599 ± 0.023
0.333CysLys: 0.333 ± 0.016
1.128CysLeu: 1.128 ± 0.029
0.236CysMet: 0.236 ± 0.012
0.359CysAsn: 0.359 ± 0.016
0.523CysPro: 0.523 ± 0.023
0.617CysGln: 0.617 ± 0.024
0.64CysArg: 0.64 ± 0.023
0.712CysSer: 0.712 ± 0.023
0.535CysThr: 0.535 ± 0.02
0.73CysVal: 0.73 ± 0.024
0.181CysTrp: 0.181 ± 0.012
0.355CysTyr: 0.355 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
4.457AspAla: 4.457 ± 0.052
0.551AspCys: 0.551 ± 0.021
2.651AspAsp: 2.651 ± 0.056
3.127AspGlu: 3.127 ± 0.054
2.103AspPhe: 2.103 ± 0.039
3.61AspGly: 3.61 ± 0.062
1.064AspHis: 1.064 ± 0.031
3.529AspIle: 3.529 ± 0.053
2.441AspLys: 2.441 ± 0.05
4.769AspLeu: 4.769 ± 0.069
1.278AspMet: 1.278 ± 0.034
2.309AspAsn: 2.309 ± 0.055
2.246AspPro: 2.246 ± 0.045
1.965AspGln: 1.965 ± 0.044
2.439AspArg: 2.439 ± 0.044
3.027AspSer: 3.027 ± 0.052
2.48AspThr: 2.48 ± 0.047
3.388AspVal: 3.388 ± 0.055
0.816AspTrp: 0.816 ± 0.029
1.911AspTyr: 1.911 ± 0.041
0.0AspXaa: 0.0 ± 0.0
Glu
4.785GluAla: 4.785 ± 0.071
0.486GluCys: 0.486 ± 0.022
2.153GluAsp: 2.153 ± 0.042
2.976GluGlu: 2.976 ± 0.055
1.83GluPhe: 1.83 ± 0.039
3.316GluGly: 3.316 ± 0.053
1.373GluHis: 1.373 ± 0.033
3.23GluIle: 3.23 ± 0.054
2.938GluLys: 2.938 ± 0.059
6.214GluLeu: 6.214 ± 0.081
1.655GluMet: 1.655 ± 0.036
2.306GluAsn: 2.306 ± 0.044
2.029GluPro: 2.029 ± 0.038
4.059GluGln: 4.059 ± 0.062
3.495GluArg: 3.495 ± 0.06
2.88GluSer: 2.88 ± 0.05
2.661GluThr: 2.661 ± 0.05
3.758GluVal: 3.758 ± 0.061
0.754GluTrp: 0.754 ± 0.023
1.493GluTyr: 1.493 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.552PheAla: 3.552 ± 0.059
0.549PheCys: 0.549 ± 0.022
2.345PheAsp: 2.345 ± 0.042
1.79PheGlu: 1.79 ± 0.039
1.665PhePhe: 1.665 ± 0.045
2.998PheGly: 2.998 ± 0.051
0.778PheHis: 0.778 ± 0.028
2.596PheIle: 2.596 ± 0.054
1.398PheLys: 1.398 ± 0.037
3.327PheLeu: 3.327 ± 0.072
0.989PheMet: 0.989 ± 0.03
1.95PheAsn: 1.95 ± 0.044
1.472PhePro: 1.472 ± 0.034
1.197PheGln: 1.197 ± 0.032
1.764PheArg: 1.764 ± 0.036
3.105PheSer: 3.105 ± 0.048
2.432PheThr: 2.432 ± 0.054
2.39PheVal: 2.39 ± 0.046
0.562PheTrp: 0.562 ± 0.023
1.264PheTyr: 1.264 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
6.004GlyAla: 6.004 ± 0.098
1.022GlyCys: 1.022 ± 0.036
3.591GlyAsp: 3.591 ± 0.067
4.306GlyGlu: 4.306 ± 0.056
3.115GlyPhe: 3.115 ± 0.057
5.448GlyGly: 5.448 ± 0.088
1.695GlyHis: 1.695 ± 0.037
4.986GlyIle: 4.986 ± 0.067
3.915GlyLys: 3.915 ± 0.064
7.394GlyLeu: 7.394 ± 0.086
2.226GlyMet: 2.226 ± 0.048
3.029GlyAsn: 3.029 ± 0.08
2.032GlyPro: 2.032 ± 0.037
3.199GlyGln: 3.199 ± 0.056
3.671GlyArg: 3.671 ± 0.052
4.567GlySer: 4.567 ± 0.098
3.957GlyThr: 3.957 ± 0.119
5.554GlyVal: 5.554 ± 0.074
1.22GlyTrp: 1.22 ± 0.032
2.608GlyTyr: 2.608 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
1.805HisAla: 1.805 ± 0.039
0.377HisCys: 0.377 ± 0.019
1.071HisAsp: 1.071 ± 0.031
1.017HisGlu: 1.017 ± 0.029
1.035HisPhe: 1.035 ± 0.031
1.764HisGly: 1.764 ± 0.039
0.875HisHis: 0.875 ± 0.034
1.351HisIle: 1.351 ± 0.035
0.752HisLys: 0.752 ± 0.024
2.453HisLeu: 2.453 ± 0.041
0.5HisMet: 0.5 ± 0.021
0.854HisAsn: 0.854 ± 0.029
1.41HisPro: 1.41 ± 0.034
1.423HisGln: 1.423 ± 0.036
1.279HisArg: 1.279 ± 0.036
1.369HisSer: 1.369 ± 0.029
1.107HisThr: 1.107 ± 0.031
1.151HisVal: 1.151 ± 0.031
0.43HisTrp: 0.43 ± 0.02
0.974HisTyr: 0.974 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.216IleAla: 6.216 ± 0.086
0.707IleCys: 0.707 ± 0.023
3.711IleAsp: 3.711 ± 0.055
3.566IleGlu: 3.566 ± 0.052
2.067IlePhe: 2.067 ± 0.049
4.761IleGly: 4.761 ± 0.065
1.171IleHis: 1.171 ± 0.03
3.418IleIle: 3.418 ± 0.059
2.685IleLys: 2.685 ± 0.049
5.173IleLeu: 5.173 ± 0.074
1.28IleMet: 1.28 ± 0.032
2.89IleAsn: 2.89 ± 0.058
2.813IlePro: 2.813 ± 0.054
2.041IleGln: 2.041 ± 0.043
2.826IleArg: 2.826 ± 0.049
4.128IleSer: 4.128 ± 0.066
3.809IleThr: 3.809 ± 0.059
3.808IleVal: 3.808 ± 0.06
0.653IleTrp: 0.653 ± 0.023
1.677IleTyr: 1.677 ± 0.041
0.0IleXaa: 0.0 ± 0.0
Lys
3.889LysAla: 3.889 ± 0.067
0.275LysCys: 0.275 ± 0.017
1.88LysAsp: 1.88 ± 0.046
2.342LysGlu: 2.342 ± 0.053
1.212LysPhe: 1.212 ± 0.037
2.746LysGly: 2.746 ± 0.052
0.892LysHis: 0.892 ± 0.026
2.455LysIle: 2.455 ± 0.054
2.174LysLys: 2.174 ± 0.051
4.508LysLeu: 4.508 ± 0.062
1.226LysMet: 1.226 ± 0.035
1.795LysAsn: 1.795 ± 0.043
2.125LysPro: 2.125 ± 0.044
2.432LysGln: 2.432 ± 0.041
2.425LysArg: 2.425 ± 0.046
2.322LysSer: 2.322 ± 0.046
2.412LysThr: 2.412 ± 0.043
3.116LysVal: 3.116 ± 0.067
0.436LysTrp: 0.436 ± 0.019
1.102LysTyr: 1.102 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
11.722LeuAla: 11.722 ± 0.119
1.264LeuCys: 1.264 ± 0.031
5.476LeuAsp: 5.476 ± 0.073
5.471LeuGlu: 5.471 ± 0.082
4.44LeuPhe: 4.44 ± 0.059
7.455LeuGly: 7.455 ± 0.09
2.373LeuHis: 2.373 ± 0.043
5.887LeuIle: 5.887 ± 0.074
4.456LeuLys: 4.456 ± 0.064
13.016LeuLeu: 13.016 ± 0.174
2.923LeuMet: 2.923 ± 0.049
4.397LeuAsn: 4.397 ± 0.081
5.995LeuPro: 5.995 ± 0.087
5.141LeuGln: 5.141 ± 0.072
5.962LeuArg: 5.962 ± 0.088
7.558LeuSer: 7.558 ± 0.086
6.426LeuThr: 6.426 ± 0.103
7.47LeuVal: 7.47 ± 0.1
1.425LeuTrp: 1.425 ± 0.038
2.759LeuTyr: 2.759 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
2.805MetAla: 2.805 ± 0.045
0.184MetCys: 0.184 ± 0.013
1.153MetAsp: 1.153 ± 0.029
1.177MetGlu: 1.177 ± 0.028
0.842MetPhe: 0.842 ± 0.025
1.888MetGly: 1.888 ± 0.043
0.476MetHis: 0.476 ± 0.019
1.474MetIle: 1.474 ± 0.038
1.274MetLys: 1.274 ± 0.033
3.098MetLeu: 3.098 ± 0.06
0.868MetMet: 0.868 ± 0.03
1.035MetAsn: 1.035 ± 0.03
1.325MetPro: 1.325 ± 0.036
1.238MetGln: 1.238 ± 0.032
1.391MetArg: 1.391 ± 0.034
1.736MetSer: 1.736 ± 0.039
1.649MetThr: 1.649 ± 0.034
1.914MetVal: 1.914 ± 0.043
0.218MetTrp: 0.218 ± 0.013
0.503MetTyr: 0.503 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.498AsnAla: 3.498 ± 0.067
0.369AsnCys: 0.369 ± 0.015
1.999AsnAsp: 1.999 ± 0.047
1.936AsnGlu: 1.936 ± 0.045
1.44AsnPhe: 1.44 ± 0.034
3.218AsnGly: 3.218 ± 0.089
0.972AsnHis: 0.972 ± 0.03
2.625AsnIle: 2.625 ± 0.057
1.662AsnLys: 1.662 ± 0.041
3.775AsnLeu: 3.775 ± 0.065
0.979AsnMet: 0.979 ± 0.028
2.001AsnAsn: 2.001 ± 0.071
2.09AsnPro: 2.09 ± 0.042
2.065AsnGln: 2.065 ± 0.052
2.009AsnArg: 2.009 ± 0.039
2.516AsnSer: 2.516 ± 0.07
2.261AsnThr: 2.261 ± 0.074
2.511AsnVal: 2.511 ± 0.059
0.595AsnTrp: 0.595 ± 0.023
1.289AsnTyr: 1.289 ± 0.032
0.0AsnXaa: 0.0 ± 0.0
Pro
4.42ProAla: 4.42 ± 0.08
0.434ProCys: 0.434 ± 0.019
2.478ProAsp: 2.478 ± 0.045
3.192ProGlu: 3.192 ± 0.06
1.775ProPhe: 1.775 ± 0.038
3.271ProGly: 3.271 ± 0.057
0.995ProHis: 0.995 ± 0.028
2.328ProIle: 2.328 ± 0.042
1.578ProLys: 1.578 ± 0.038
5.412ProLeu: 5.412 ± 0.082
1.088ProMet: 1.088 ± 0.028
1.46ProAsn: 1.46 ± 0.034
1.742ProPro: 1.742 ± 0.04
2.501ProGln: 2.501 ± 0.048
1.866ProArg: 1.866 ± 0.044
2.472ProSer: 2.472 ± 0.042
2.354ProThr: 2.354 ± 0.044
3.581ProVal: 3.581 ± 0.065
0.681ProTrp: 0.681 ± 0.03
1.331ProTyr: 1.331 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
5.245GlnAla: 5.245 ± 0.076
0.443GlnCys: 0.443 ± 0.02
2.006GlnAsp: 2.006 ± 0.039
2.379GlnGlu: 2.379 ± 0.046
1.614GlnPhe: 1.614 ± 0.037
3.784GlnGly: 3.784 ± 0.078
1.452GlnHis: 1.452 ± 0.034
2.586GlnIle: 2.586 ± 0.048
1.748GlnLys: 1.748 ± 0.041
6.136GlnLeu: 6.136 ± 0.085
1.243GlnMet: 1.243 ± 0.034
1.492GlnAsn: 1.492 ± 0.036
2.553GlnPro: 2.553 ± 0.057
4.774GlnGln: 4.774 ± 0.109
3.436GlnArg: 3.436 ± 0.052
2.574GlnSer: 2.574 ± 0.049
2.273GlnThr: 2.273 ± 0.042
3.666GlnVal: 3.666 ± 0.056
0.8GlnTrp: 0.8 ± 0.024
1.332GlnTyr: 1.332 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
4.212ArgAla: 4.212 ± 0.061
0.6ArgCys: 0.6 ± 0.024
2.746ArgAsp: 2.746 ± 0.054
3.265ArgGlu: 3.265 ± 0.063
2.434ArgPhe: 2.434 ± 0.054
3.205ArgGly: 3.205 ± 0.053
1.503ArgHis: 1.503 ± 0.037
3.283ArgIle: 3.283 ± 0.057
2.169ArgLys: 2.169 ± 0.041
6.29ArgLeu: 6.29 ± 0.084
1.366ArgMet: 1.366 ± 0.029
1.918ArgAsn: 1.918 ± 0.038
2.149ArgPro: 2.149 ± 0.04
3.23ArgGln: 3.23 ± 0.06
3.133ArgArg: 3.133 ± 0.065
2.828ArgSer: 2.828 ± 0.054
2.335ArgThr: 2.335 ± 0.045
3.614ArgVal: 3.614 ± 0.061
0.903ArgTrp: 0.903 ± 0.032
2.063ArgTyr: 2.063 ± 0.048
0.0ArgXaa: 0.0 ± 0.0
Ser
5.496SerAla: 5.496 ± 0.071
0.654SerCys: 0.654 ± 0.026
3.055SerAsp: 3.055 ± 0.053
3.239SerGlu: 3.239 ± 0.052
2.347SerPhe: 2.347 ± 0.044
5.381SerGly: 5.381 ± 0.096
1.483SerHis: 1.483 ± 0.035
3.299SerIle: 3.299 ± 0.057
2.289SerLys: 2.289 ± 0.047
7.008SerLeu: 7.008 ± 0.09
1.52SerMet: 1.52 ± 0.035
2.316SerAsn: 2.316 ± 0.057
2.76SerPro: 2.76 ± 0.048
2.931SerGln: 2.931 ± 0.063
3.145SerArg: 3.145 ± 0.052
3.977SerSer: 3.977 ± 0.068
3.127SerThr: 3.127 ± 0.067
4.18SerVal: 4.18 ± 0.075
0.932SerTrp: 0.932 ± 0.03
1.841SerTyr: 1.841 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
5.062ThrAla: 5.062 ± 0.074
0.502ThrCys: 0.502 ± 0.018
2.599ThrAsp: 2.599 ± 0.06
2.737ThrGlu: 2.737 ± 0.048
1.986ThrPhe: 1.986 ± 0.045
4.437ThrGly: 4.437 ± 0.104
1.187ThrHis: 1.187 ± 0.033
2.985ThrIle: 2.985 ± 0.058
1.606ThrLys: 1.606 ± 0.038
7.547ThrLeu: 7.547 ± 0.112
1.047ThrMet: 1.047 ± 0.029
1.71ThrAsn: 1.71 ± 0.054
3.166ThrPro: 3.166 ± 0.055
2.422ThrGln: 2.422 ± 0.051
2.626ThrArg: 2.626 ± 0.048
3.029ThrSer: 3.029 ± 0.059
3.169ThrThr: 3.169 ± 0.071
3.977ThrVal: 3.977 ± 0.079
0.764ThrTrp: 0.764 ± 0.025
1.337ThrTyr: 1.337 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
6.573ValAla: 6.573 ± 0.084
0.759ValCys: 0.759 ± 0.023
3.667ValAsp: 3.667 ± 0.068
3.941ValGlu: 3.941 ± 0.058
2.481ValPhe: 2.481 ± 0.042
4.835ValGly: 4.835 ± 0.075
1.234ValHis: 1.234 ± 0.032
4.659ValIle: 4.659 ± 0.063
3.083ValLys: 3.083 ± 0.056
7.409ValLeu: 7.409 ± 0.084
2.136ValMet: 2.136 ± 0.039
3.004ValAsn: 3.004 ± 0.066
2.98ValPro: 2.98 ± 0.05
2.414ValGln: 2.414 ± 0.049
3.453ValArg: 3.453 ± 0.051
4.42ValSer: 4.42 ± 0.061
4.213ValThr: 4.213 ± 0.068
5.505ValVal: 5.505 ± 0.074
0.837ValTrp: 0.837 ± 0.027
1.709ValTyr: 1.709 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
0.936TrpAla: 0.936 ± 0.027
0.205TrpCys: 0.205 ± 0.012
0.625TrpAsp: 0.625 ± 0.023
0.538TrpGlu: 0.538 ± 0.02
0.62TrpPhe: 0.62 ± 0.024
0.888TrpGly: 0.888 ± 0.028
0.427TrpHis: 0.427 ± 0.019
0.666TrpIle: 0.666 ± 0.028
0.422TrpLys: 0.422 ± 0.021
2.284TrpLeu: 2.284 ± 0.048
0.395TrpMet: 0.395 ± 0.017
0.453TrpAsn: 0.453 ± 0.021
0.623TrpPro: 0.623 ± 0.026
1.284TrpGln: 1.284 ± 0.042
0.95TrpArg: 0.95 ± 0.031
0.804TrpSer: 0.804 ± 0.029
0.473TrpThr: 0.473 ± 0.019
0.968TrpVal: 0.968 ± 0.031
0.246TrpTrp: 0.246 ± 0.013
0.403TrpTyr: 0.403 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.429TyrAla: 2.429 ± 0.05
0.406TyrCys: 0.406 ± 0.019
1.51TyrAsp: 1.51 ± 0.036
1.08TyrGlu: 1.08 ± 0.031
1.239TyrPhe: 1.239 ± 0.032
2.22TyrGly: 2.22 ± 0.05
0.856TyrHis: 0.856 ± 0.025
1.536TyrIle: 1.536 ± 0.036
0.963TyrLys: 0.963 ± 0.031
3.404TyrLeu: 3.404 ± 0.06
0.586TyrMet: 0.586 ± 0.021
1.047TyrAsn: 1.047 ± 0.037
1.484TyrPro: 1.484 ± 0.035
2.038TyrGln: 2.038 ± 0.042
1.999TyrArg: 1.999 ± 0.042
1.82TyrSer: 1.82 ± 0.04
1.468TyrThr: 1.468 ± 0.033
1.641TyrVal: 1.641 ± 0.032
0.499TyrTrp: 0.499 ± 0.023
0.986TyrTyr: 0.986 ± 0.029
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4028 proteins (1306886 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski