Amino acid dipepetide frequency for Enterococcus phage IME_EF3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.713AlaAla: 0.713 ± 0.34
0.317AlaCys: 0.317 ± 0.187
3.647AlaAsp: 3.647 ± 0.47
3.409AlaGlu: 3.409 ± 0.466
2.14AlaPhe: 2.14 ± 0.392
2.854AlaGly: 2.854 ± 0.581
1.11AlaHis: 1.11 ± 0.302
4.44AlaIle: 4.44 ± 0.966
6.184AlaLys: 6.184 ± 0.712
4.836AlaLeu: 4.836 ± 0.736
2.378AlaMet: 2.378 ± 0.576
3.647AlaAsn: 3.647 ± 0.549
2.14AlaPro: 2.14 ± 0.288
1.506AlaGln: 1.506 ± 0.299
1.586AlaArg: 1.586 ± 0.327
3.092AlaSer: 3.092 ± 0.588
4.043AlaThr: 4.043 ± 0.478
4.677AlaVal: 4.677 ± 0.544
0.476AlaTrp: 0.476 ± 0.215
2.616AlaTyr: 2.616 ± 0.485
0.0AlaXaa: 0.0 ± 0.0
Cys
0.396CysAla: 0.396 ± 0.2
0.0CysCys: 0.0 ± 0.0
0.634CysAsp: 0.634 ± 0.264
0.872CysGlu: 0.872 ± 0.295
0.0CysPhe: 0.0 ± 0.0
0.317CysGly: 0.317 ± 0.142
0.317CysHis: 0.317 ± 0.189
0.555CysIle: 0.555 ± 0.266
0.872CysLys: 0.872 ± 0.302
0.396CysLeu: 0.396 ± 0.203
0.476CysMet: 0.476 ± 0.208
0.793CysAsn: 0.793 ± 0.304
0.0CysPro: 0.0 ± 0.0
0.159CysGln: 0.159 ± 0.143
0.317CysArg: 0.317 ± 0.172
0.555CysSer: 0.555 ± 0.198
0.159CysThr: 0.159 ± 0.112
0.396CysVal: 0.396 ± 0.192
0.079CysTrp: 0.079 ± 0.077
0.079CysTyr: 0.079 ± 0.094
0.0CysXaa: 0.0 ± 0.0
Asp
2.854AspAla: 2.854 ± 0.517
0.396AspCys: 0.396 ± 0.219
2.22AspAsp: 2.22 ± 0.511
4.677AspGlu: 4.677 ± 0.635
2.933AspPhe: 2.933 ± 0.559
5.708AspGly: 5.708 ± 0.757
0.634AspHis: 0.634 ± 0.206
5.074AspIle: 5.074 ± 0.715
6.659AspLys: 6.659 ± 0.802
5.787AspLeu: 5.787 ± 0.77
1.982AspMet: 1.982 ± 0.322
4.519AspAsn: 4.519 ± 0.706
2.14AspPro: 2.14 ± 0.399
1.348AspGln: 1.348 ± 0.315
2.22AspArg: 2.22 ± 0.421
3.25AspSer: 3.25 ± 0.467
3.885AspThr: 3.885 ± 0.672
3.726AspVal: 3.726 ± 0.658
0.872AspTrp: 0.872 ± 0.268
3.171AspTyr: 3.171 ± 0.794
0.0AspXaa: 0.0 ± 0.0
Glu
4.677GluAla: 4.677 ± 0.766
0.793GluCys: 0.793 ± 0.329
4.677GluAsp: 4.677 ± 0.912
7.293GluGlu: 7.293 ± 1.51
3.25GluPhe: 3.25 ± 0.545
3.885GluGly: 3.885 ± 0.557
1.586GluHis: 1.586 ± 0.442
4.202GluIle: 4.202 ± 0.558
5.866GluLys: 5.866 ± 0.668
8.879GluLeu: 8.879 ± 0.803
2.616GluMet: 2.616 ± 0.509
4.043GluAsn: 4.043 ± 0.524
2.616GluPro: 2.616 ± 0.74
3.726GluGln: 3.726 ± 0.685
3.25GluArg: 3.25 ± 0.537
3.964GluSer: 3.964 ± 0.567
3.964GluThr: 3.964 ± 0.481
6.501GluVal: 6.501 ± 0.662
1.427GluTrp: 1.427 ± 0.336
3.567GluTyr: 3.567 ± 0.676
0.0GluXaa: 0.0 ± 0.0
Phe
1.348PheAla: 1.348 ± 0.302
0.317PheCys: 0.317 ± 0.154
3.013PheAsp: 3.013 ± 0.521
2.854PheGlu: 2.854 ± 0.56
0.951PhePhe: 0.951 ± 0.357
2.616PheGly: 2.616 ± 0.498
0.476PheHis: 0.476 ± 0.207
3.885PheIle: 3.885 ± 0.603
4.281PheLys: 4.281 ± 0.561
2.378PheLeu: 2.378 ± 0.467
1.11PheMet: 1.11 ± 0.299
3.33PheAsn: 3.33 ± 0.493
0.713PhePro: 0.713 ± 0.226
1.744PheGln: 1.744 ± 0.376
1.586PheArg: 1.586 ± 0.326
2.22PheSer: 2.22 ± 0.456
3.567PheThr: 3.567 ± 0.695
2.458PheVal: 2.458 ± 0.549
0.476PheTrp: 0.476 ± 0.171
1.348PheTyr: 1.348 ± 0.374
0.0PheXaa: 0.0 ± 0.0
Gly
4.598GlyAla: 4.598 ± 1.211
0.396GlyCys: 0.396 ± 0.247
3.171GlyAsp: 3.171 ± 0.505
3.409GlyGlu: 3.409 ± 0.445
3.33GlyPhe: 3.33 ± 0.408
4.44GlyGly: 4.44 ± 1.049
0.476GlyHis: 0.476 ± 0.178
5.312GlyIle: 5.312 ± 0.903
5.708GlyLys: 5.708 ± 0.707
5.549GlyLeu: 5.549 ± 0.671
1.823GlyMet: 1.823 ± 0.366
3.726GlyAsn: 3.726 ± 0.649
0.476GlyPro: 0.476 ± 0.217
2.061GlyGln: 2.061 ± 0.35
2.458GlyArg: 2.458 ± 0.369
3.409GlySer: 3.409 ± 0.583
3.092GlyThr: 3.092 ± 0.61
4.598GlyVal: 4.598 ± 0.621
1.189GlyTrp: 1.189 ± 0.251
3.171GlyTyr: 3.171 ± 0.608
0.0GlyXaa: 0.0 ± 0.0
His
0.634HisAla: 0.634 ± 0.192
0.317HisCys: 0.317 ± 0.162
0.713HisAsp: 0.713 ± 0.195
0.634HisGlu: 0.634 ± 0.246
0.634HisPhe: 0.634 ± 0.261
1.031HisGly: 1.031 ± 0.345
0.476HisHis: 0.476 ± 0.219
0.793HisIle: 0.793 ± 0.218
1.665HisLys: 1.665 ± 0.384
0.793HisLeu: 0.793 ± 0.22
0.238HisMet: 0.238 ± 0.13
1.586HisAsn: 1.586 ± 0.364
0.159HisPro: 0.159 ± 0.116
0.555HisGln: 0.555 ± 0.2
1.189HisArg: 1.189 ± 0.486
0.872HisSer: 0.872 ± 0.336
0.793HisThr: 0.793 ± 0.341
1.11HisVal: 1.11 ± 0.339
0.0HisTrp: 0.0 ± 0.0
0.951HisTyr: 0.951 ± 0.261
0.0HisXaa: 0.0 ± 0.0
Ile
3.964IleAla: 3.964 ± 0.498
0.476IleCys: 0.476 ± 0.217
5.232IleAsp: 5.232 ± 0.502
6.58IleGlu: 6.58 ± 1.086
2.458IlePhe: 2.458 ± 0.386
3.805IleGly: 3.805 ± 0.798
0.713IleHis: 0.713 ± 0.237
3.885IleIle: 3.885 ± 0.481
6.342IleLys: 6.342 ± 0.714
4.836IleLeu: 4.836 ± 0.635
1.506IleMet: 1.506 ± 0.353
4.202IleAsn: 4.202 ± 0.572
3.013IlePro: 3.013 ± 0.448
2.695IleGln: 2.695 ± 0.492
1.744IleArg: 1.744 ± 0.278
3.964IleSer: 3.964 ± 0.511
4.598IleThr: 4.598 ± 0.559
4.202IleVal: 4.202 ± 0.47
0.793IleTrp: 0.793 ± 0.254
1.903IleTyr: 1.903 ± 0.43
0.0IleXaa: 0.0 ± 0.0
Lys
6.818LysAla: 6.818 ± 0.957
0.872LysCys: 0.872 ± 0.393
5.708LysAsp: 5.708 ± 0.66
8.245LysGlu: 8.245 ± 0.922
3.33LysPhe: 3.33 ± 0.451
5.629LysGly: 5.629 ± 0.714
1.189LysHis: 1.189 ± 0.335
4.757LysIle: 4.757 ± 0.621
6.739LysLys: 6.739 ± 0.774
7.69LysLeu: 7.69 ± 0.801
3.171LysMet: 3.171 ± 0.449
5.47LysAsn: 5.47 ± 0.746
2.854LysPro: 2.854 ± 0.604
3.805LysGln: 3.805 ± 0.477
3.805LysArg: 3.805 ± 0.561
3.726LysSer: 3.726 ± 0.684
5.391LysThr: 5.391 ± 0.564
5.787LysVal: 5.787 ± 0.608
1.268LysTrp: 1.268 ± 0.302
3.488LysTyr: 3.488 ± 0.506
0.0LysXaa: 0.0 ± 0.0
Leu
4.122LeuAla: 4.122 ± 0.587
0.555LeuCys: 0.555 ± 0.222
6.659LeuAsp: 6.659 ± 0.675
8.324LeuGlu: 8.324 ± 0.954
3.25LeuPhe: 3.25 ± 0.451
4.836LeuGly: 4.836 ± 0.671
0.951LeuHis: 0.951 ± 0.298
4.757LeuIle: 4.757 ± 0.638
7.69LeuLys: 7.69 ± 0.968
7.373LeuLeu: 7.373 ± 0.829
1.823LeuMet: 1.823 ± 0.314
5.549LeuAsn: 5.549 ± 0.68
3.092LeuPro: 3.092 ± 0.501
4.202LeuGln: 4.202 ± 0.644
2.695LeuArg: 2.695 ± 0.538
4.122LeuSer: 4.122 ± 0.493
4.043LeuThr: 4.043 ± 0.52
5.549LeuVal: 5.549 ± 0.75
1.11LeuTrp: 1.11 ± 0.271
2.775LeuTyr: 2.775 ± 0.609
0.0LeuXaa: 0.0 ± 0.0
Met
1.268MetAla: 1.268 ± 0.336
0.317MetCys: 0.317 ± 0.193
1.665MetAsp: 1.665 ± 0.368
2.378MetGlu: 2.378 ± 0.429
1.11MetPhe: 1.11 ± 0.333
1.903MetGly: 1.903 ± 0.427
0.317MetHis: 0.317 ± 0.182
1.665MetIle: 1.665 ± 0.352
2.775MetLys: 2.775 ± 0.513
1.744MetLeu: 1.744 ± 0.392
0.396MetMet: 0.396 ± 0.188
1.665MetAsn: 1.665 ± 0.362
0.555MetPro: 0.555 ± 0.223
1.189MetGln: 1.189 ± 0.258
1.665MetArg: 1.665 ± 0.358
1.903MetSer: 1.903 ± 0.374
1.823MetThr: 1.823 ± 0.447
1.744MetVal: 1.744 ± 0.47
0.396MetTrp: 0.396 ± 0.221
1.823MetTyr: 1.823 ± 0.432
0.0MetXaa: 0.0 ± 0.0
Asn
4.757AsnAla: 4.757 ± 0.694
0.317AsnCys: 0.317 ± 0.199
3.805AsnAsp: 3.805 ± 0.568
5.549AsnGlu: 5.549 ± 0.574
1.823AsnPhe: 1.823 ± 0.42
6.263AsnGly: 6.263 ± 0.806
0.793AsnHis: 0.793 ± 0.254
4.202AsnIle: 4.202 ± 0.638
5.629AsnLys: 5.629 ± 0.747
4.757AsnLeu: 4.757 ± 0.627
1.427AsnMet: 1.427 ± 0.297
3.488AsnAsn: 3.488 ± 0.525
1.823AsnPro: 1.823 ± 0.401
1.744AsnGln: 1.744 ± 0.332
1.823AsnArg: 1.823 ± 0.436
3.171AsnSer: 3.171 ± 0.514
4.836AsnThr: 4.836 ± 0.73
3.647AsnVal: 3.647 ± 0.484
0.872AsnTrp: 0.872 ± 0.308
3.25AsnTyr: 3.25 ± 0.711
0.0AsnXaa: 0.0 ± 0.0
Pro
1.427ProAla: 1.427 ± 0.342
0.079ProCys: 0.079 ± 0.081
2.299ProAsp: 2.299 ± 0.366
3.726ProGlu: 3.726 ± 0.58
1.189ProPhe: 1.189 ± 0.237
0.159ProGly: 0.159 ± 0.102
0.159ProHis: 0.159 ± 0.11
1.823ProIle: 1.823 ± 0.356
2.061ProLys: 2.061 ± 0.629
2.854ProLeu: 2.854 ± 0.437
0.634ProMet: 0.634 ± 0.258
2.22ProAsn: 2.22 ± 0.439
0.476ProPro: 0.476 ± 0.204
1.348ProGln: 1.348 ± 0.3
1.11ProArg: 1.11 ± 0.336
1.982ProSer: 1.982 ± 0.357
1.903ProThr: 1.903 ± 0.373
1.982ProVal: 1.982 ± 0.301
0.238ProTrp: 0.238 ± 0.124
1.903ProTyr: 1.903 ± 0.482
0.0ProXaa: 0.0 ± 0.0
Gln
2.378GlnAla: 2.378 ± 0.48
0.317GlnCys: 0.317 ± 0.153
2.061GlnAsp: 2.061 ± 0.354
2.695GlnGlu: 2.695 ± 0.465
1.586GlnPhe: 1.586 ± 0.339
1.903GlnGly: 1.903 ± 0.353
0.476GlnHis: 0.476 ± 0.196
2.775GlnIle: 2.775 ± 0.375
2.061GlnLys: 2.061 ± 0.422
3.092GlnLeu: 3.092 ± 0.533
1.11GlnMet: 1.11 ± 0.262
1.506GlnAsn: 1.506 ± 0.296
1.506GlnPro: 1.506 ± 0.363
2.061GlnGln: 2.061 ± 0.436
2.14GlnArg: 2.14 ± 0.538
2.616GlnSer: 2.616 ± 0.508
1.903GlnThr: 1.903 ± 0.36
3.013GlnVal: 3.013 ± 0.452
0.634GlnTrp: 0.634 ± 0.252
2.22GlnTyr: 2.22 ± 0.362
0.0GlnXaa: 0.0 ± 0.0
Arg
1.665ArgAla: 1.665 ± 0.4
0.476ArgCys: 0.476 ± 0.265
2.616ArgAsp: 2.616 ± 0.493
1.982ArgGlu: 1.982 ± 0.493
1.823ArgPhe: 1.823 ± 0.379
1.744ArgGly: 1.744 ± 0.445
0.951ArgHis: 0.951 ± 0.382
2.537ArgIle: 2.537 ± 0.458
3.33ArgLys: 3.33 ± 0.606
3.171ArgLeu: 3.171 ± 0.509
1.268ArgMet: 1.268 ± 0.313
3.013ArgAsn: 3.013 ± 0.456
0.872ArgPro: 0.872 ± 0.238
1.586ArgGln: 1.586 ± 0.416
1.427ArgArg: 1.427 ± 0.371
1.427ArgSer: 1.427 ± 0.339
1.823ArgThr: 1.823 ± 0.374
2.458ArgVal: 2.458 ± 0.476
0.317ArgTrp: 0.317 ± 0.186
1.427ArgTyr: 1.427 ± 0.354
0.0ArgXaa: 0.0 ± 0.0
Ser
2.933SerAla: 2.933 ± 0.582
0.0SerCys: 0.0 ± 0.0
3.33SerAsp: 3.33 ± 0.473
3.964SerGlu: 3.964 ± 0.483
3.013SerPhe: 3.013 ± 0.422
4.44SerGly: 4.44 ± 0.903
1.665SerHis: 1.665 ± 0.353
3.488SerIle: 3.488 ± 0.559
4.836SerLys: 4.836 ± 0.924
3.33SerLeu: 3.33 ± 0.511
1.348SerMet: 1.348 ± 0.351
3.409SerAsn: 3.409 ± 0.482
0.872SerPro: 0.872 ± 0.207
2.458SerGln: 2.458 ± 0.452
1.268SerArg: 1.268 ± 0.297
2.14SerSer: 2.14 ± 0.432
4.043SerThr: 4.043 ± 0.731
3.33SerVal: 3.33 ± 0.651
0.793SerTrp: 0.793 ± 0.237
2.537SerTyr: 2.537 ± 0.537
0.0SerXaa: 0.0 ± 0.0
Thr
3.25ThrAla: 3.25 ± 0.46
0.238ThrCys: 0.238 ± 0.155
3.409ThrAsp: 3.409 ± 0.604
3.964ThrGlu: 3.964 ± 0.569
2.14ThrPhe: 2.14 ± 0.396
4.44ThrGly: 4.44 ± 0.656
1.268ThrHis: 1.268 ± 0.302
4.915ThrIle: 4.915 ± 0.636
6.421ThrLys: 6.421 ± 0.746
5.549ThrLeu: 5.549 ± 0.822
1.744ThrMet: 1.744 ± 0.334
3.092ThrAsn: 3.092 ± 0.517
2.854ThrPro: 2.854 ± 0.573
2.14ThrGln: 2.14 ± 0.377
1.823ThrArg: 1.823 ± 0.398
2.458ThrSer: 2.458 ± 0.429
4.202ThrThr: 4.202 ± 0.742
4.36ThrVal: 4.36 ± 0.437
0.634ThrTrp: 0.634 ± 0.213
2.22ThrTyr: 2.22 ± 0.57
0.0ThrXaa: 0.0 ± 0.0
Val
5.47ValAla: 5.47 ± 0.719
0.317ValCys: 0.317 ± 0.149
5.312ValAsp: 5.312 ± 0.611
4.757ValGlu: 4.757 ± 0.729
3.171ValPhe: 3.171 ± 0.459
4.36ValGly: 4.36 ± 0.734
1.031ValHis: 1.031 ± 0.288
3.885ValIle: 3.885 ± 0.549
5.47ValLys: 5.47 ± 1.096
5.232ValLeu: 5.232 ± 0.577
2.061ValMet: 2.061 ± 0.306
3.964ValAsn: 3.964 ± 0.603
1.903ValPro: 1.903 ± 0.346
2.22ValGln: 2.22 ± 0.517
2.299ValArg: 2.299 ± 0.453
5.866ValSer: 5.866 ± 0.716
3.567ValThr: 3.567 ± 0.7
4.122ValVal: 4.122 ± 0.443
0.634ValTrp: 0.634 ± 0.235
2.14ValTyr: 2.14 ± 0.444
0.0ValXaa: 0.0 ± 0.0
Trp
0.396TrpAla: 0.396 ± 0.164
0.159TrpCys: 0.159 ± 0.109
0.555TrpAsp: 0.555 ± 0.21
1.348TrpGlu: 1.348 ± 0.26
0.793TrpPhe: 0.793 ± 0.288
0.555TrpGly: 0.555 ± 0.244
0.159TrpHis: 0.159 ± 0.106
0.713TrpIle: 0.713 ± 0.26
0.951TrpLys: 0.951 ± 0.276
1.348TrpLeu: 1.348 ± 0.326
0.159TrpMet: 0.159 ± 0.124
0.951TrpAsn: 0.951 ± 0.345
0.0TrpPro: 0.0 ± 0.0
0.396TrpGln: 0.396 ± 0.183
0.555TrpArg: 0.555 ± 0.244
0.634TrpSer: 0.634 ± 0.193
0.951TrpThr: 0.951 ± 0.266
1.427TrpVal: 1.427 ± 0.281
0.396TrpTrp: 0.396 ± 0.215
0.317TrpTyr: 0.317 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.14TyrAla: 2.14 ± 0.431
0.713TyrCys: 0.713 ± 0.226
3.33TyrAsp: 3.33 ± 0.537
4.122TyrGlu: 4.122 ± 0.634
1.744TyrPhe: 1.744 ± 0.318
1.506TyrGly: 1.506 ± 0.383
0.396TyrHis: 0.396 ± 0.13
3.25TyrIle: 3.25 ± 0.645
4.281TyrLys: 4.281 ± 0.578
3.885TyrLeu: 3.885 ± 0.736
0.951TyrMet: 0.951 ± 0.271
3.805TyrAsn: 3.805 ± 0.579
1.506TyrPro: 1.506 ± 0.405
1.11TyrGln: 1.11 ± 0.36
0.951TyrArg: 0.951 ± 0.301
1.823TyrSer: 1.823 ± 0.479
2.537TyrThr: 2.537 ± 0.556
2.695TyrVal: 2.695 ± 0.527
0.159TyrTrp: 0.159 ± 0.125
2.061TyrTyr: 2.061 ± 0.509
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (12615 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski