Leucobacter chromiiresistens
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2423 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A147ERP8|A0A147ERP8_9MICO Inner-membrane translocator OS=Leucobacter chromiiresistens OX=1079994 GN=NS354_01550 PE=4 SV=1
AA1 pKa = 7.62 AAQAVFDD8 pKa = 4.62 RR9 pKa = 11.84 VNADD13 pKa = 2.98 GGIDD17 pKa = 3.6 GRR19 pKa = 11.84 QIEE22 pKa = 4.89 YY23 pKa = 8.1 TTVDD27 pKa = 3.93 DD28 pKa = 5.25 KK29 pKa = 11.91 GDD31 pKa = 3.93 PASATAAARR40 pKa = 11.84 QLVGSDD46 pKa = 3.24 EE47 pKa = 4.38 VVALVGGASLIEE59 pKa = 4.09 CEE61 pKa = 4.66 INQEE65 pKa = 4.1 YY66 pKa = 9.34 YY67 pKa = 10.44 AQEE70 pKa = 4.26 NVLSMPGIGVDD81 pKa = 3.66 TGCFDD86 pKa = 4.21 SPNIAPANLGPFNDD100 pKa = 3.7 MTLTLLNGSEE110 pKa = 4.17 NLGLDD115 pKa = 4.57 DD116 pKa = 4.09 ICVLLEE122 pKa = 3.83 IAGSTKK128 pKa = 9.4 PAYY131 pKa = 10.17 LAGVEE136 pKa = 4.09 KK137 pKa = 8.66 WTEE140 pKa = 3.72 ITGEE144 pKa = 4.12 EE145 pKa = 4.41 PLLVDD150 pKa = 5.28 DD151 pKa = 4.65 SLPYY155 pKa = 10.49 GGSDD159 pKa = 3.23 YY160 pKa = 11.14 TPYY163 pKa = 10.17 IVKK166 pKa = 10.28 AKK168 pKa = 10.48 NAGCSAIAVNGIEE181 pKa = 4.51 PDD183 pKa = 3.82 AIGQVKK189 pKa = 9.47 AAQAQGWDD197 pKa = 3.32 DD198 pKa = 3.54 VTFLFLTSVYY208 pKa = 10.7 SEE210 pKa = 4.39 NFASALDD217 pKa = 3.5 WTGAGVHH224 pKa = 5.68 VPAEE228 pKa = 4.43 FYY230 pKa = 10.62 PFTEE234 pKa = 4.6 DD235 pKa = 4.48 NEE237 pKa = 4.43 INADD241 pKa = 3.28 WKK243 pKa = 11.41 SLMEE247 pKa = 4.66 EE248 pKa = 3.68 NDD250 pKa = 3.1 IALTSFSQGGYY261 pKa = 9.66 LAATHH266 pKa = 5.91 LVEE269 pKa = 4.56 VLSGMEE275 pKa = 3.95 GEE277 pKa = 4.29 ITRR280 pKa = 11.84 EE281 pKa = 3.87 TVAEE285 pKa = 4.34 ALHH288 pKa = 6.71 GMDD291 pKa = 5.32 PIEE294 pKa = 4.72 NPMVAAPYY302 pKa = 10.56 QFDD305 pKa = 3.22 QVAAQEE311 pKa = 4.19 YY312 pKa = 8.57 TPGGWPVVLEE322 pKa = 4.21 SGTRR326 pKa = 11.84 AWKK329 pKa = 10.33 QSADD333 pKa = 3.24 DD334 pKa = 3.78 WLIPSSS340 pKa = 3.62
Molecular weight: 36.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.541
ProMoST 3.897
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.643
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.872
Patrickios 0.922
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.782
Protein with the highest isoelectric point:
>tr|A0A147ERN6|A0A147ERN6_9MICO Nitrilase OS=Leucobacter chromiiresistens OX=1079994 GN=NS354_00710 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.5 VHH17 pKa = 5.46 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIVAARR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.45 GRR40 pKa = 11.84 SKK42 pKa = 10.13 LTAA45 pKa = 4.04
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2423
0
2423
786420
27
3966
324.6
34.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.173 ± 0.078
0.51 ± 0.01
5.908 ± 0.04
6.206 ± 0.051
3.203 ± 0.036
9.155 ± 0.046
1.989 ± 0.025
4.613 ± 0.039
1.75 ± 0.034
10.023 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.778 ± 0.022
1.876 ± 0.028
5.29 ± 0.035
2.903 ± 0.024
7.549 ± 0.056
5.63 ± 0.037
5.574 ± 0.043
8.626 ± 0.04
1.343 ± 0.019
1.903 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here