Streptococcus phage P5652
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A286QP55|A0A286QP55_9CAUD Primase OS=Streptococcus phage P5652 OX=1971420 GN=P5652_35 PE=4 SV=1
MM1 pKa = 7.13 VNWVDD6 pKa = 4.58 ANGNDD11 pKa = 3.63 IPDD14 pKa = 4.12 GADD17 pKa = 3.05 QEE19 pKa = 5.1 FKK21 pKa = 11.28 AGMFFSFAGDD31 pKa = 3.56 EE32 pKa = 4.15 NNITDD37 pKa = 3.82 TGEE40 pKa = 3.65 GGYY43 pKa = 10.61 YY44 pKa = 9.76 GGYY47 pKa = 8.0 YY48 pKa = 9.41 YY49 pKa = 10.76 RR50 pKa = 11.84 RR51 pKa = 11.84 FEE53 pKa = 4.06 FGQFGTVWLSCWNKK67 pKa = 10.66 DD68 pKa = 3.89 DD69 pKa = 5.14 LVNYY73 pKa = 7.38 YY74 pKa = 8.26 QQ75 pKa = 4.22
Molecular weight: 8.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.835
IPC2_protein 3.821
IPC_protein 3.745
Toseland 3.541
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.732
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.681
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.617
EMBOSS 3.732
Sillero 3.872
Patrickios 0.223
IPC_peptide 3.719
IPC2_peptide 3.834
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A286QNG4|A0A286QNG4_9CAUD Distal tail protein OS=Streptococcus phage P5652 OX=1971420 GN=P5652_16 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.56 KK3 pKa = 10.13 LFNWIWSRR11 pKa = 11.84 KK12 pKa = 5.23 QTEE15 pKa = 4.26 TPTWTFEE22 pKa = 4.31 NNASEE27 pKa = 4.32 PSRR30 pKa = 11.84 DD31 pKa = 3.5 RR32 pKa = 11.84 YY33 pKa = 10.39 NRR35 pKa = 11.84 AHH37 pKa = 7.03 GLGKK41 pKa = 8.42 TLII44 pKa = 4.16
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.157
IPC2_protein 9.443
IPC_protein 9.619
Toseland 10.467
ProMoST 9.984
Dawson 10.555
Bjellqvist 10.16
Wikipedia 10.672
Rodwell 11.052
Grimsley 10.584
Solomon 10.613
Lehninger 10.613
Nozaki 10.409
DTASelect 10.16
Thurlkill 10.438
EMBOSS 10.833
Sillero 10.467
Patrickios 10.906
IPC_peptide 10.628
IPC2_peptide 8.609
IPC2.peptide.svr19 8.61
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
43
0
43
10312
38
1591
239.8
27.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.429 ± 0.529
0.533 ± 0.137
6.72 ± 0.289
6.284 ± 0.46
4.228 ± 0.241
6.885 ± 0.523
1.513 ± 0.1
6.555 ± 0.289
8.029 ± 0.499
7.981 ± 0.358
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.434 ± 0.211
6.497 ± 0.301
3.035 ± 0.178
4.024 ± 0.287
4.257 ± 0.345
6.158 ± 0.247
6.585 ± 0.28
6.206 ± 0.247
1.59 ± 0.179
4.054 ± 0.295
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here