Opuntia virus 2

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus; unclassified Tobamovirus

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2U8U8G9|A0A2U8U8G9_9VIRU Movement protein OS=Opuntia virus 2 OX=2200716 GN=MP PE=3 SV=2
MM1 pKa = 7.87PYY3 pKa = 10.24VGVSTRR9 pKa = 11.84SLALLTPSFVRR20 pKa = 11.84YY21 pKa = 10.08VDD23 pKa = 3.67VLSLIQSILGSQSFQTQSGRR43 pKa = 11.84DD44 pKa = 3.43QLRR47 pKa = 11.84SQLAGLLTAPASRR60 pKa = 11.84DD61 pKa = 3.09VRR63 pKa = 11.84FPEE66 pKa = 3.69EE67 pKa = 4.0TVFVRR72 pKa = 11.84VFDD75 pKa = 4.47SVIEE79 pKa = 3.97PLWLSVLAATDD90 pKa = 3.21TRR92 pKa = 11.84NRR94 pKa = 11.84IIEE97 pKa = 4.12EE98 pKa = 4.01QMVQGPTTPEE108 pKa = 3.48VLNATKK114 pKa = 10.38RR115 pKa = 11.84VDD117 pKa = 3.71DD118 pKa = 3.79ATVACRR124 pKa = 11.84TALSKK129 pKa = 10.71LRR131 pKa = 11.84SGISDD136 pKa = 3.58NLDD139 pKa = 3.34TVCYY143 pKa = 10.5DD144 pKa = 3.2RR145 pKa = 11.84TKK147 pKa = 11.05FEE149 pKa = 4.99LEE151 pKa = 4.68LGSAWLQPGGAPGGGNPAGSGARR174 pKa = 11.84TGTSTT179 pKa = 4.32

Molecular weight: 19.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2U8U8C4|A0A2U8U8C4_9VIRU Isoform of A0A2U8U8C2 128 kDa hypothetical replicase OS=Opuntia virus 2 OX=2200716 GN=RdRp PE=4 SV=2
MM1 pKa = 7.95AIDD4 pKa = 4.37TYY6 pKa = 11.74SKK8 pKa = 10.76LLEE11 pKa = 4.11PKK13 pKa = 10.34RR14 pKa = 11.84FIKK17 pKa = 10.47PGFWYY22 pKa = 7.55CTPLGKK28 pKa = 10.25SKK30 pKa = 11.18SPLKK34 pKa = 10.43FCNICVTDD42 pKa = 3.59IVKK45 pKa = 10.31VKK47 pKa = 10.67SVGSFSFCLSVYY59 pKa = 9.02KK60 pKa = 9.27TVRR63 pKa = 11.84PDD65 pKa = 2.84HH66 pKa = 7.05RR67 pKa = 11.84SFKK70 pKa = 9.95FARR73 pKa = 11.84LVGVTISGVWHH84 pKa = 6.78FDD86 pKa = 2.72KK87 pKa = 10.91GLPGSAVIALIDD99 pKa = 4.49SRR101 pKa = 11.84ITDD104 pKa = 3.49VNLSILSKK112 pKa = 10.42VVVSASQAGEE122 pKa = 3.92FQFTFSPNFSITMNDD137 pKa = 3.58LKK139 pKa = 10.91RR140 pKa = 11.84NPIEE144 pKa = 5.56LIVDD148 pKa = 4.16LQGLPLCDD156 pKa = 3.32GFEE159 pKa = 4.2PLSLEE164 pKa = 4.26LGFLFYY170 pKa = 11.17YY171 pKa = 10.33SDD173 pKa = 3.84SALSSSLGAKK183 pKa = 9.62IKK185 pKa = 10.51LLQSEE190 pKa = 4.91TNVEE194 pKa = 4.11SGDD197 pKa = 3.8IPDD200 pKa = 3.86HH201 pKa = 6.41VFDD204 pKa = 4.33EE205 pKa = 4.94VVSEE209 pKa = 4.07VDD211 pKa = 3.83LNSVIPKK218 pKa = 9.94ARR220 pKa = 11.84NLRR223 pKa = 11.84NALSVSSKK231 pKa = 10.59SSSALEE237 pKa = 3.74RR238 pKa = 11.84AVVSNKK244 pKa = 8.61FAKK247 pKa = 9.81GAKK250 pKa = 9.07ANKK253 pKa = 9.49RR254 pKa = 3.56

Molecular weight: 27.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

1

4

3131

179

1598

782.8

87.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.557 ± 0.361

2.236 ± 0.181

6.292 ± 0.219

5.078 ± 0.239

5.717 ± 0.331

5.206 ± 0.42

2.587 ± 0.492

4.919 ± 0.184

5.685 ± 0.48

9.997 ± 0.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.98 ± 0.299

4.056 ± 0.241

4.503 ± 0.169

3.513 ± 0.279

5.43 ± 0.281

9.358 ± 0.721

5.206 ± 0.472

8.879 ± 0.375

1.054 ± 0.055

2.715 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski