Streptococcus satellite phage Javan343

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLE1|A0A4D5ZLE1_9VIRU DNA primase OS=Streptococcus satellite phage Javan343 OX=2558651 GN=JavanS343_0008 PE=4 SV=1
MM1 pKa = 7.86PSFDD5 pKa = 4.12EE6 pKa = 5.84LINSDD11 pKa = 4.51QEE13 pKa = 4.85FEE15 pKa = 4.71DD16 pKa = 4.36SPISLKK22 pKa = 11.05LKK24 pKa = 10.62DD25 pKa = 3.51ILKK28 pKa = 9.01QLKK31 pKa = 10.28VNTEE35 pKa = 3.84EE36 pKa = 5.24LEE38 pKa = 4.07

Molecular weight: 4.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZKQ3|A0A4D5ZKQ3_9VIRU HTH DNA-binding protein OS=Streptococcus satellite phage Javan343 OX=2558651 GN=JavanS343_0003 PE=4 SV=1
MM1 pKa = 7.65TEE3 pKa = 3.66PAYY6 pKa = 10.74DD7 pKa = 3.54GMKK10 pKa = 10.03LAKK13 pKa = 9.57EE14 pKa = 4.55DD15 pKa = 3.43KK16 pKa = 10.45TKK18 pKa = 11.04DD19 pKa = 3.18RR20 pKa = 11.84RR21 pKa = 11.84PKK23 pKa = 10.42LKK25 pKa = 9.87QLKK28 pKa = 8.74EE29 pKa = 3.84RR30 pKa = 11.84KK31 pKa = 8.27QLKK34 pKa = 9.73KK35 pKa = 10.38LRR37 pKa = 11.84KK38 pKa = 8.98KK39 pKa = 10.02RR40 pKa = 11.84RR41 pKa = 11.84KK42 pKa = 9.88GSS44 pKa = 3.23

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2186

38

536

156.1

17.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.81 ± 0.369

0.457 ± 0.168

5.947 ± 0.578

8.371 ± 0.946

3.568 ± 0.513

5.489 ± 0.528

1.189 ± 0.265

6.679 ± 0.567

10.247 ± 0.84

10.567 ± 0.941

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.194

4.3 ± 0.38

3.431 ± 0.636

4.392 ± 0.394

4.941 ± 0.454

5.947 ± 0.513

5.764 ± 0.599

5.124 ± 0.557

0.823 ± 0.149

4.666 ± 0.552
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski