Amino acid dipepetide frequency for Salmonella phage SE19

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.37AlaAla: 9.37 ± 1.504
0.618AlaCys: 0.618 ± 0.141
4.291AlaAsp: 4.291 ± 0.341
5.698AlaGlu: 5.698 ± 0.463
2.986AlaPhe: 2.986 ± 0.33
5.526AlaGly: 5.526 ± 0.511
1.133AlaHis: 1.133 ± 0.205
4.702AlaIle: 4.702 ± 0.374
7.174AlaLys: 7.174 ± 0.525
6.693AlaLeu: 6.693 ± 0.521
2.059AlaMet: 2.059 ± 0.345
3.741AlaAsn: 3.741 ± 0.39
2.643AlaPro: 2.643 ± 0.334
3.398AlaGln: 3.398 ± 0.448
3.295AlaArg: 3.295 ± 0.309
4.668AlaSer: 4.668 ± 0.62
4.325AlaThr: 4.325 ± 0.591
4.771AlaVal: 4.771 ± 0.375
0.824AlaTrp: 0.824 ± 0.19
2.54AlaTyr: 2.54 ± 0.319
0.0AlaXaa: 0.0 ± 0.0
Cys
0.412CysAla: 0.412 ± 0.102
0.206CysCys: 0.206 ± 0.09
0.618CysAsp: 0.618 ± 0.132
0.652CysGlu: 0.652 ± 0.15
0.515CysPhe: 0.515 ± 0.148
0.961CysGly: 0.961 ± 0.189
0.172CysHis: 0.172 ± 0.064
0.618CysIle: 0.618 ± 0.12
0.824CysLys: 0.824 ± 0.168
0.858CysLeu: 0.858 ± 0.16
0.172CysMet: 0.172 ± 0.068
0.481CysAsn: 0.481 ± 0.142
0.412CysPro: 0.412 ± 0.113
0.309CysGln: 0.309 ± 0.127
0.446CysArg: 0.446 ± 0.133
0.686CysSer: 0.686 ± 0.158
0.481CysThr: 0.481 ± 0.122
0.584CysVal: 0.584 ± 0.147
0.069CysTrp: 0.069 ± 0.045
0.275CysTyr: 0.275 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
4.668AspAla: 4.668 ± 0.44
0.481AspCys: 0.481 ± 0.149
3.192AspAsp: 3.192 ± 0.437
5.011AspGlu: 5.011 ± 0.446
2.883AspPhe: 2.883 ± 0.31
3.604AspGly: 3.604 ± 0.377
0.995AspHis: 0.995 ± 0.178
4.599AspIle: 4.599 ± 0.437
4.462AspLys: 4.462 ± 0.385
5.286AspLeu: 5.286 ± 0.402
2.368AspMet: 2.368 ± 0.269
2.574AspAsn: 2.574 ± 0.296
3.158AspPro: 3.158 ± 0.3
1.682AspGln: 1.682 ± 0.254
2.643AspArg: 2.643 ± 0.314
3.776AspSer: 3.776 ± 0.424
4.119AspThr: 4.119 ± 0.393
4.188AspVal: 4.188 ± 0.383
0.892AspTrp: 0.892 ± 0.186
2.986AspTyr: 2.986 ± 0.372
0.0AspXaa: 0.0 ± 0.0
Glu
5.869GluAla: 5.869 ± 0.405
0.686GluCys: 0.686 ± 0.176
3.982GluAsp: 3.982 ± 0.357
5.492GluGlu: 5.492 ± 0.535
2.712GluPhe: 2.712 ± 0.3
3.707GluGly: 3.707 ± 0.33
1.682GluHis: 1.682 ± 0.224
5.252GluIle: 5.252 ± 0.367
4.977GluLys: 4.977 ± 0.471
7.139GluLeu: 7.139 ± 0.483
2.506GluMet: 2.506 ± 0.354
2.437GluAsn: 2.437 ± 0.276
1.648GluPro: 1.648 ± 0.261
3.123GluGln: 3.123 ± 0.454
3.295GluArg: 3.295 ± 0.386
3.501GluSer: 3.501 ± 0.251
4.222GluThr: 4.222 ± 0.42
4.222GluVal: 4.222 ± 0.386
0.927GluTrp: 0.927 ± 0.201
2.883GluTyr: 2.883 ± 0.297
0.0GluXaa: 0.0 ± 0.0
Phe
2.609PheAla: 2.609 ± 0.257
0.412PheCys: 0.412 ± 0.125
2.849PheAsp: 2.849 ± 0.34
2.78PheGlu: 2.78 ± 0.352
1.476PhePhe: 1.476 ± 0.221
2.952PheGly: 2.952 ± 0.354
1.201PheHis: 1.201 ± 0.247
2.712PheIle: 2.712 ± 0.338
3.021PheLys: 3.021 ± 0.287
2.918PheLeu: 2.918 ± 0.315
0.995PheMet: 0.995 ± 0.191
2.849PheAsn: 2.849 ± 0.3
1.751PhePro: 1.751 ± 0.259
1.03PheGln: 1.03 ± 0.162
1.922PheArg: 1.922 ± 0.306
2.712PheSer: 2.712 ± 0.303
2.471PheThr: 2.471 ± 0.242
2.677PheVal: 2.677 ± 0.268
0.481PheTrp: 0.481 ± 0.126
1.51PheTyr: 1.51 ± 0.195
0.0PheXaa: 0.0 ± 0.0
Gly
4.599GlyAla: 4.599 ± 0.576
0.927GlyCys: 0.927 ± 0.201
3.844GlyAsp: 3.844 ± 0.445
5.011GlyGlu: 5.011 ± 0.371
2.918GlyPhe: 2.918 ± 0.298
3.57GlyGly: 3.57 ± 0.403
1.304GlyHis: 1.304 ± 0.257
4.462GlyIle: 4.462 ± 0.395
5.08GlyLys: 5.08 ± 0.37
4.496GlyLeu: 4.496 ± 0.434
1.888GlyMet: 1.888 ± 0.228
3.844GlyAsn: 3.844 ± 0.353
0.858GlyPro: 0.858 ± 0.15
2.677GlyGln: 2.677 ± 0.301
2.952GlyArg: 2.952 ± 0.287
3.879GlySer: 3.879 ± 0.464
3.501GlyThr: 3.501 ± 0.39
4.805GlyVal: 4.805 ± 0.366
0.927GlyTrp: 0.927 ± 0.171
3.158GlyTyr: 3.158 ± 0.287
0.0GlyXaa: 0.0 ± 0.0
His
1.098HisAla: 1.098 ± 0.204
0.275HisCys: 0.275 ± 0.085
1.373HisAsp: 1.373 ± 0.236
0.995HisGlu: 0.995 ± 0.169
0.721HisPhe: 0.721 ± 0.165
1.201HisGly: 1.201 ± 0.204
0.652HisHis: 0.652 ± 0.16
1.613HisIle: 1.613 ± 0.216
1.098HisLys: 1.098 ± 0.196
1.648HisLeu: 1.648 ± 0.233
0.343HisMet: 0.343 ± 0.14
0.892HisAsn: 0.892 ± 0.224
0.927HisPro: 0.927 ± 0.204
0.412HisGln: 0.412 ± 0.131
1.03HisArg: 1.03 ± 0.178
1.339HisSer: 1.339 ± 0.214
0.755HisThr: 0.755 ± 0.177
0.652HisVal: 0.652 ± 0.152
0.172HisTrp: 0.172 ± 0.082
0.858HisTyr: 0.858 ± 0.171
0.0HisXaa: 0.0 ± 0.0
Ile
5.252IleAla: 5.252 ± 0.395
0.652IleCys: 0.652 ± 0.167
5.183IleAsp: 5.183 ± 0.449
4.222IleGlu: 4.222 ± 0.387
2.3IlePhe: 2.3 ± 0.298
4.188IleGly: 4.188 ± 0.409
1.133IleHis: 1.133 ± 0.164
3.776IleIle: 3.776 ± 0.299
5.046IleLys: 5.046 ± 0.456
4.84IleLeu: 4.84 ± 0.381
1.922IleMet: 1.922 ± 0.264
3.844IleAsn: 3.844 ± 0.403
2.609IlePro: 2.609 ± 0.334
2.197IleGln: 2.197 ± 0.315
2.609IleArg: 2.609 ± 0.234
3.947IleSer: 3.947 ± 0.31
4.565IleThr: 4.565 ± 0.443
3.913IleVal: 3.913 ± 0.365
0.789IleTrp: 0.789 ± 0.175
2.162IleTyr: 2.162 ± 0.275
0.0IleXaa: 0.0 ± 0.0
Lys
6.762LysAla: 6.762 ± 0.571
0.412LysCys: 0.412 ± 0.146
4.84LysAsp: 4.84 ± 0.426
5.046LysGlu: 5.046 ± 0.414
3.432LysPhe: 3.432 ± 0.312
3.501LysGly: 3.501 ± 0.35
1.236LysHis: 1.236 ± 0.239
3.501LysIle: 3.501 ± 0.408
4.153LysLys: 4.153 ± 0.546
6.968LysLeu: 6.968 ± 0.526
2.643LysMet: 2.643 ± 0.336
3.295LysAsn: 3.295 ± 0.372
2.746LysPro: 2.746 ± 0.399
3.123LysGln: 3.123 ± 0.324
3.398LysArg: 3.398 ± 0.292
4.119LysSer: 4.119 ± 0.393
3.982LysThr: 3.982 ± 0.448
4.874LysVal: 4.874 ± 0.417
0.652LysTrp: 0.652 ± 0.148
3.089LysTyr: 3.089 ± 0.26
0.0LysXaa: 0.0 ± 0.0
Leu
7.036LeuAla: 7.036 ± 0.536
0.721LeuCys: 0.721 ± 0.14
6.796LeuAsp: 6.796 ± 0.429
7.208LeuGlu: 7.208 ± 0.52
2.849LeuPhe: 2.849 ± 0.319
5.286LeuGly: 5.286 ± 0.574
1.545LeuHis: 1.545 ± 0.224
4.634LeuIle: 4.634 ± 0.437
5.938LeuLys: 5.938 ± 0.453
6.144LeuLeu: 6.144 ± 0.586
1.888LeuMet: 1.888 ± 0.268
5.011LeuAsn: 5.011 ± 0.397
3.432LeuPro: 3.432 ± 0.36
3.158LeuGln: 3.158 ± 0.333
3.776LeuArg: 3.776 ± 0.31
5.114LeuSer: 5.114 ± 0.444
4.428LeuThr: 4.428 ± 0.341
5.252LeuVal: 5.252 ± 0.483
0.652LeuTrp: 0.652 ± 0.162
2.746LeuTyr: 2.746 ± 0.282
0.0LeuXaa: 0.0 ± 0.0
Met
1.922MetAla: 1.922 ± 0.319
0.309MetCys: 0.309 ± 0.101
1.27MetAsp: 1.27 ± 0.203
2.197MetGlu: 2.197 ± 0.299
0.892MetPhe: 0.892 ± 0.208
1.648MetGly: 1.648 ± 0.209
0.515MetHis: 0.515 ± 0.142
2.059MetIle: 2.059 ± 0.289
2.506MetLys: 2.506 ± 0.333
2.368MetLeu: 2.368 ± 0.34
0.549MetMet: 0.549 ± 0.128
1.236MetAsn: 1.236 ± 0.203
0.858MetPro: 0.858 ± 0.157
1.098MetGln: 1.098 ± 0.211
1.098MetArg: 1.098 ± 0.208
2.265MetSer: 2.265 ± 0.273
1.922MetThr: 1.922 ± 0.244
1.167MetVal: 1.167 ± 0.208
0.309MetTrp: 0.309 ± 0.101
0.961MetTyr: 0.961 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
3.879AsnAla: 3.879 ± 0.467
0.549AsnCys: 0.549 ± 0.165
2.883AsnAsp: 2.883 ± 0.337
2.712AsnGlu: 2.712 ± 0.309
2.094AsnPhe: 2.094 ± 0.261
4.359AsnGly: 4.359 ± 0.533
0.858AsnHis: 0.858 ± 0.184
3.947AsnIle: 3.947 ± 0.43
3.638AsnLys: 3.638 ± 0.341
4.702AsnLeu: 4.702 ± 0.375
1.442AsnMet: 1.442 ± 0.214
2.334AsnAsn: 2.334 ± 0.28
2.334AsnPro: 2.334 ± 0.281
1.442AsnGln: 1.442 ± 0.199
2.128AsnArg: 2.128 ± 0.255
3.501AsnSer: 3.501 ± 0.321
2.918AsnThr: 2.918 ± 0.293
3.535AsnVal: 3.535 ± 0.379
0.686AsnTrp: 0.686 ± 0.156
1.613AsnTyr: 1.613 ± 0.24
0.0AsnXaa: 0.0 ± 0.0
Pro
2.3ProAla: 2.3 ± 0.305
0.343ProCys: 0.343 ± 0.117
2.3ProAsp: 2.3 ± 0.314
3.329ProGlu: 3.329 ± 0.364
1.751ProPhe: 1.751 ± 0.256
2.059ProGly: 2.059 ± 0.261
0.446ProHis: 0.446 ± 0.13
1.991ProIle: 1.991 ± 0.335
2.059ProLys: 2.059 ± 0.261
2.506ProLeu: 2.506 ± 0.242
0.584ProMet: 0.584 ± 0.142
2.403ProAsn: 2.403 ± 0.339
1.407ProPro: 1.407 ± 0.245
1.064ProGln: 1.064 ± 0.204
1.545ProArg: 1.545 ± 0.207
2.094ProSer: 2.094 ± 0.273
2.265ProThr: 2.265 ± 0.282
2.609ProVal: 2.609 ± 0.263
0.584ProTrp: 0.584 ± 0.137
1.51ProTyr: 1.51 ± 0.196
0.0ProXaa: 0.0 ± 0.0
Gln
3.123GlnAla: 3.123 ± 0.495
0.446GlnCys: 0.446 ± 0.129
1.956GlnAsp: 1.956 ± 0.295
2.403GlnGlu: 2.403 ± 0.388
1.819GlnPhe: 1.819 ± 0.243
1.956GlnGly: 1.956 ± 0.212
0.618GlnHis: 0.618 ± 0.151
2.128GlnIle: 2.128 ± 0.258
2.643GlnLys: 2.643 ± 0.398
3.776GlnLeu: 3.776 ± 0.407
0.824GlnMet: 0.824 ± 0.144
1.442GlnAsn: 1.442 ± 0.2
0.481GlnPro: 0.481 ± 0.16
1.785GlnGln: 1.785 ± 0.301
1.751GlnArg: 1.751 ± 0.236
2.471GlnSer: 2.471 ± 0.274
1.991GlnThr: 1.991 ± 0.249
2.952GlnVal: 2.952 ± 0.293
0.515GlnTrp: 0.515 ± 0.164
1.476GlnTyr: 1.476 ± 0.227
0.0GlnXaa: 0.0 ± 0.0
Arg
3.295ArgAla: 3.295 ± 0.339
0.206ArgCys: 0.206 ± 0.08
2.746ArgAsp: 2.746 ± 0.282
3.089ArgGlu: 3.089 ± 0.345
1.682ArgPhe: 1.682 ± 0.267
3.501ArgGly: 3.501 ± 0.32
0.515ArgHis: 0.515 ± 0.133
3.089ArgIle: 3.089 ± 0.335
2.746ArgLys: 2.746 ± 0.405
3.741ArgLeu: 3.741 ± 0.34
1.407ArgMet: 1.407 ± 0.229
2.403ArgAsn: 2.403 ± 0.293
1.27ArgPro: 1.27 ± 0.21
1.613ArgGln: 1.613 ± 0.237
2.437ArgArg: 2.437 ± 0.354
2.368ArgSer: 2.368 ± 0.241
2.574ArgThr: 2.574 ± 0.269
3.398ArgVal: 3.398 ± 0.315
0.412ArgTrp: 0.412 ± 0.112
1.785ArgTyr: 1.785 ± 0.309
0.0ArgXaa: 0.0 ± 0.0
Ser
4.84SerAla: 4.84 ± 0.818
0.618SerCys: 0.618 ± 0.164
3.261SerAsp: 3.261 ± 0.336
3.741SerGlu: 3.741 ± 0.44
3.089SerPhe: 3.089 ± 0.307
4.462SerGly: 4.462 ± 0.409
0.858SerHis: 0.858 ± 0.156
4.599SerIle: 4.599 ± 0.495
4.359SerLys: 4.359 ± 0.41
5.595SerLeu: 5.595 ± 0.402
1.407SerMet: 1.407 ± 0.203
3.123SerAsn: 3.123 ± 0.327
1.991SerPro: 1.991 ± 0.295
1.51SerGln: 1.51 ± 0.232
2.643SerArg: 2.643 ± 0.313
4.359SerSer: 4.359 ± 0.42
3.673SerThr: 3.673 ± 0.312
4.222SerVal: 4.222 ± 0.334
1.201SerTrp: 1.201 ± 0.196
2.54SerTyr: 2.54 ± 0.305
0.0SerXaa: 0.0 ± 0.0
Thr
4.805ThrAla: 4.805 ± 0.564
0.515ThrCys: 0.515 ± 0.122
3.158ThrAsp: 3.158 ± 0.322
3.329ThrGlu: 3.329 ± 0.33
2.403ThrPhe: 2.403 ± 0.298
5.046ThrGly: 5.046 ± 0.433
0.961ThrHis: 0.961 ± 0.166
3.947ThrIle: 3.947 ± 0.396
3.879ThrLys: 3.879 ± 0.389
4.565ThrLeu: 4.565 ± 0.387
1.27ThrMet: 1.27 ± 0.21
3.913ThrAsn: 3.913 ± 0.431
2.54ThrPro: 2.54 ± 0.251
2.334ThrGln: 2.334 ± 0.294
2.574ThrArg: 2.574 ± 0.313
3.844ThrSer: 3.844 ± 0.361
2.918ThrThr: 2.918 ± 0.313
4.393ThrVal: 4.393 ± 0.474
0.549ThrTrp: 0.549 ± 0.144
2.059ThrTyr: 2.059 ± 0.256
0.0ThrXaa: 0.0 ± 0.0
Val
5.252ValAla: 5.252 ± 0.439
0.584ValCys: 0.584 ± 0.155
4.84ValAsp: 4.84 ± 0.437
4.462ValGlu: 4.462 ± 0.355
2.368ValPhe: 2.368 ± 0.27
4.016ValGly: 4.016 ± 0.374
0.892ValHis: 0.892 ± 0.181
4.462ValIle: 4.462 ± 0.405
4.805ValLys: 4.805 ± 0.337
4.977ValLeu: 4.977 ± 0.377
1.579ValMet: 1.579 ± 0.186
3.055ValAsn: 3.055 ± 0.373
2.712ValPro: 2.712 ± 0.302
2.506ValGln: 2.506 ± 0.307
2.643ValArg: 2.643 ± 0.266
4.085ValSer: 4.085 ± 0.364
4.702ValThr: 4.702 ± 0.47
4.188ValVal: 4.188 ± 0.441
0.481ValTrp: 0.481 ± 0.145
2.677ValTyr: 2.677 ± 0.272
0.0ValXaa: 0.0 ± 0.0
Trp
0.515TrpAla: 0.515 ± 0.124
0.172TrpCys: 0.172 ± 0.084
0.961TrpAsp: 0.961 ± 0.196
0.892TrpGlu: 0.892 ± 0.157
0.515TrpPhe: 0.515 ± 0.144
0.652TrpGly: 0.652 ± 0.121
0.172TrpHis: 0.172 ± 0.07
0.686TrpIle: 0.686 ± 0.156
0.858TrpLys: 0.858 ± 0.16
1.442TrpLeu: 1.442 ± 0.25
0.343TrpMet: 0.343 ± 0.118
0.515TrpAsn: 0.515 ± 0.135
0.172TrpPro: 0.172 ± 0.082
0.584TrpGln: 0.584 ± 0.137
0.549TrpArg: 0.549 ± 0.144
0.824TrpSer: 0.824 ± 0.222
0.652TrpThr: 0.652 ± 0.144
0.686TrpVal: 0.686 ± 0.134
0.206TrpTrp: 0.206 ± 0.107
0.378TrpTyr: 0.378 ± 0.102
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.849TyrAla: 2.849 ± 0.309
0.618TyrCys: 0.618 ± 0.128
3.226TyrAsp: 3.226 ± 0.328
2.025TyrGlu: 2.025 ± 0.258
1.922TyrPhe: 1.922 ± 0.298
2.506TyrGly: 2.506 ± 0.337
1.167TyrHis: 1.167 ± 0.168
2.368TyrIle: 2.368 ± 0.26
2.506TyrLys: 2.506 ± 0.333
2.952TyrLeu: 2.952 ± 0.338
0.927TyrMet: 0.927 ± 0.173
2.162TyrAsn: 2.162 ± 0.244
1.201TyrPro: 1.201 ± 0.198
1.51TyrGln: 1.51 ± 0.256
1.545TyrArg: 1.545 ± 0.239
2.54TyrSer: 2.54 ± 0.271
2.54TyrThr: 2.54 ± 0.263
2.197TyrVal: 2.197 ± 0.311
0.446TyrTrp: 0.446 ± 0.124
1.304TyrTyr: 1.304 ± 0.265
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 144 proteins (29135 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski