Treponema pallidum (strain Nichols)

Taxonomy: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Spirochaetales; Treponemataceae; Treponema; Treponema pallidum; Treponema pallidum subsp. pallidum

Average proteome isoelectric point is 7.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1027 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O83962|O83962_TREPA Protease IV (SppA) OS=Treponema pallidum (strain Nichols) OX=243276 GN=TP_0997 PE=4 SV=1
MM1 pKa = 6.89GTYY4 pKa = 8.91MCDD7 pKa = 3.14LCGWGYY13 pKa = 10.59NPEE16 pKa = 4.04VGDD19 pKa = 4.09ADD21 pKa = 3.79GGIPAGTAFEE31 pKa = 4.32NLPDD35 pKa = 3.34HH36 pKa = 6.76WEE38 pKa = 4.33CPLCGVDD45 pKa = 2.96KK46 pKa = 10.93TSFVKK51 pKa = 10.71VV52 pKa = 3.37

Molecular weight: 5.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O83367|O83367_TREPA Uncharacterized protein OS=Treponema pallidum (strain Nichols) OX=243276 GN=TP_0347 PE=4 SV=1
MM1 pKa = 7.63SYY3 pKa = 10.43SCSAEE8 pKa = 4.07STSVCSVHH16 pKa = 6.76FSVFRR21 pKa = 11.84IPGATCRR28 pKa = 11.84CDD30 pKa = 3.58VFCAEE35 pKa = 4.78HH36 pKa = 7.07VFFCARR42 pKa = 11.84SQLKK46 pKa = 10.15RR47 pKa = 11.84IVRR50 pKa = 11.84ALCVNGHH57 pKa = 4.91TAKK60 pKa = 10.67FSRR63 pKa = 11.84PLHH66 pKa = 5.11VRR68 pKa = 11.84DD69 pKa = 3.09RR70 pKa = 11.84VSFEE74 pKa = 3.84WVRR77 pKa = 11.84SVPPALIPEE86 pKa = 4.68NISLSILFEE95 pKa = 4.38NEE97 pKa = 4.32DD98 pKa = 3.58IIAVNKK104 pKa = 7.63AQGMIVHH111 pKa = 7.34PGAGHH116 pKa = 4.79WTGTLVQALSFYY128 pKa = 10.25RR129 pKa = 11.84VYY131 pKa = 10.56RR132 pKa = 11.84ARR134 pKa = 11.84FEE136 pKa = 5.42DD137 pKa = 3.8EE138 pKa = 4.73FSRR141 pKa = 11.84QFQKK145 pKa = 11.01GFPDD149 pKa = 4.22FFSTLRR155 pKa = 11.84QGIVHH160 pKa = 7.31RR161 pKa = 11.84LDD163 pKa = 3.82KK164 pKa = 10.49DD165 pKa = 3.56TSGVLLTSRR174 pKa = 11.84NMHH177 pKa = 5.44AHH179 pKa = 5.89EE180 pKa = 4.27ALVRR184 pKa = 11.84SFKK187 pKa = 10.35KK188 pKa = 9.21RR189 pKa = 11.84QVRR192 pKa = 11.84KK193 pKa = 10.21VYY195 pKa = 10.26LALLQGVPARR205 pKa = 11.84GVGVIEE211 pKa = 3.98TTIVRR216 pKa = 11.84DD217 pKa = 3.35RR218 pKa = 11.84RR219 pKa = 11.84RR220 pKa = 11.84RR221 pKa = 11.84TRR223 pKa = 11.84FVASEE228 pKa = 4.17DD229 pKa = 3.77FSKK232 pKa = 11.1GKK234 pKa = 9.35YY235 pKa = 9.45ARR237 pKa = 11.84TRR239 pKa = 11.84YY240 pKa = 10.07KK241 pKa = 10.76VMKK244 pKa = 10.03ICGACAFVQFLLDD257 pKa = 3.09TGRR260 pKa = 11.84THH262 pKa = 7.41QIRR265 pKa = 11.84VHH267 pKa = 6.06ARR269 pKa = 11.84YY270 pKa = 9.46LGCPVVGDD278 pKa = 3.73PLYY281 pKa = 10.96GSRR284 pKa = 11.84NICGIPTTLMLHH296 pKa = 6.71AYY298 pKa = 9.31AVRR301 pKa = 11.84FVLPRR306 pKa = 11.84TKK308 pKa = 10.59KK309 pKa = 10.45RR310 pKa = 11.84ITLVAPIPLRR320 pKa = 11.84FVRR323 pKa = 11.84LIHH326 pKa = 6.22RR327 pKa = 11.84LSVRR331 pKa = 3.29

Molecular weight: 37.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1027

0

1027

349599

30

1533

340.4

37.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.124 ± 0.093

1.91 ± 0.041

4.528 ± 0.05

5.981 ± 0.082

4.452 ± 0.061

6.957 ± 0.073

2.757 ± 0.05

4.907 ± 0.065

3.979 ± 0.064

10.152 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.092 ± 0.036

2.48 ± 0.036

4.203 ± 0.057

3.845 ± 0.046

7.431 ± 0.077

6.622 ± 0.068

5.299 ± 0.049

8.26 ± 0.081

0.977 ± 0.025

3.033 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski