Enterococcus phage IME-EF4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; Enterococcus virus EF4

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5URL2|V5URL2_9CAUD Uncharacterized protein OS=Enterococcus phage IME-EF4 OX=1432658 GN=IME-EF4_17 PE=4 SV=1
MM1 pKa = 6.97TVKK4 pKa = 9.88TNYY7 pKa = 9.87YY8 pKa = 9.42ICLTNNEE15 pKa = 4.58LFTLYY20 pKa = 10.13TEE22 pKa = 4.3EE23 pKa = 5.74PILVMYY29 pKa = 9.04EE30 pKa = 3.86KK31 pKa = 10.61AVEE34 pKa = 3.98NDD36 pKa = 3.07EE37 pKa = 5.09KK38 pKa = 11.13LLKK41 pKa = 10.46LEE43 pKa = 4.21KK44 pKa = 10.36PEE46 pKa = 4.73AIEE49 pKa = 5.19IDD51 pKa = 4.12GEE53 pKa = 4.31MQDD56 pKa = 3.57TFITIPLDD64 pKa = 3.76SILYY68 pKa = 8.73VLEE71 pKa = 4.49DD72 pKa = 3.8AKK74 pKa = 11.25CC75 pKa = 3.22

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5UPT2|V5UPT2_9CAUD Transcriptional regulator OS=Enterococcus phage IME-EF4 OX=1432658 GN=IME-EF4_13 PE=4 SV=1
MM1 pKa = 7.58TKK3 pKa = 9.38TKK5 pKa = 9.92KK6 pKa = 10.25QYY8 pKa = 10.59IIINSEE14 pKa = 3.99TKK16 pKa = 9.61RR17 pKa = 11.84GYY19 pKa = 10.88AEE21 pKa = 4.65RR22 pKa = 11.84EE23 pKa = 3.97TFMEE27 pKa = 4.16AVEE30 pKa = 4.19VAKK33 pKa = 10.62RR34 pKa = 11.84KK35 pKa = 10.23VNGQVITRR43 pKa = 11.84KK44 pKa = 9.97DD45 pKa = 3.0WEE47 pKa = 4.24AMKK50 pKa = 10.62KK51 pKa = 10.07

Molecular weight: 6.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

12114

51

1410

201.9

22.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.869 ± 0.382

0.603 ± 0.113

6.158 ± 0.235

8.222 ± 0.451

4.07 ± 0.206

6.298 ± 0.597

1.469 ± 0.158

6.885 ± 0.209

8.833 ± 0.396

8.172 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.873 ± 0.178

6.464 ± 0.279

2.873 ± 0.191

3.797 ± 0.148

3.5 ± 0.247

5.374 ± 0.255

6.332 ± 0.351

6.777 ± 0.233

1.271 ± 0.105

4.16 ± 0.327
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski