Lactococcus phage PLgT-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A182BQA9|A0A182BQA9_9CAUD Uncharacterized protein OS=Lactococcus phage PLgT-1 OX=1815955 GN=PlgT1_42 PE=4 SV=1
MM1 pKa = 7.45TKK3 pKa = 10.21FEE5 pKa = 4.61EE6 pKa = 4.32EE7 pKa = 3.71FNYY10 pKa = 10.85LIEE13 pKa = 4.47LSGKK17 pKa = 8.6VLIGQVDD24 pKa = 3.87AEE26 pKa = 4.12AFEE29 pKa = 4.57KK30 pKa = 10.87NRR32 pKa = 11.84IAFFEE37 pKa = 4.36RR38 pKa = 11.84YY39 pKa = 7.64EE40 pKa = 4.05TDD42 pKa = 2.96QQQALPVVPEE52 pKa = 5.18CVAEE56 pKa = 4.18WIEE59 pKa = 4.02YY60 pKa = 8.77VKK62 pKa = 10.72KK63 pKa = 10.79DD64 pKa = 3.65EE65 pKa = 4.85LFNALGLLDD74 pKa = 4.71NDD76 pKa = 4.39NMPKK80 pKa = 10.55DD81 pKa = 3.44VDD83 pKa = 3.13EE84 pKa = 4.34WLFLQSNDD92 pKa = 4.43DD93 pKa = 4.31NINLILRR100 pKa = 11.84AWLDD104 pKa = 3.67GYY106 pKa = 7.82QVKK109 pKa = 10.12KK110 pKa = 9.95PQLFYY115 pKa = 11.27LRR117 pKa = 11.84DD118 pKa = 3.52EE119 pKa = 4.34LTGQFLAKK127 pKa = 10.49DD128 pKa = 3.7NQFKK132 pKa = 11.22DD133 pKa = 2.9KK134 pKa = 10.86DD135 pKa = 3.83RR136 pKa = 11.84YY137 pKa = 10.22FFWTGADD144 pKa = 3.81PLTHH148 pKa = 7.11SIGTAWKK155 pKa = 8.74LTFTQEE161 pKa = 4.56EE162 pKa = 4.36IDD164 pKa = 3.79SMEE167 pKa = 4.23TGSYY171 pKa = 8.7EE172 pKa = 4.03QIEE175 pKa = 4.34AEE177 pKa = 4.26EE178 pKa = 4.1

Molecular weight: 20.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172LN91|A0A172LN91_9CAUD Uncharacterized protein OS=Lactococcus phage PLgT-1 OX=1815955 GN=PlgT1_15 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.12RR3 pKa = 11.84NKK5 pKa = 9.36GRR7 pKa = 11.84EE8 pKa = 4.14WLGGPALVILVIMAPFAVILFVQIITAIYY37 pKa = 9.86LYY39 pKa = 10.95LL40 pKa = 5.5

Molecular weight: 4.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

11594

40

1593

175.7

19.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.004 ± 0.434

0.474 ± 0.097

6.27 ± 0.234

7.098 ± 0.437

3.916 ± 0.205

6.434 ± 0.529

1.268 ± 0.155

7.021 ± 0.313

9.263 ± 0.425

8.021 ± 0.278

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.708 ± 0.174

6.236 ± 0.298

2.769 ± 0.127

4.149 ± 0.262

3.579 ± 0.23

6.736 ± 0.448

5.727 ± 0.381

5.986 ± 0.311

1.354 ± 0.166

3.985 ± 0.319
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski