Wallerfield virus

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses; Negevirus

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W5VNC4|W5VNC4_9VIRU Uncharacterized protein OS=Wallerfield virus OX=1457165 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84SIYY5 pKa = 10.52CFLLFALTMATGKK18 pKa = 10.19EE19 pKa = 3.93SRR21 pKa = 11.84DD22 pKa = 3.47YY23 pKa = 11.08FLASMGRR30 pKa = 11.84NLKK33 pKa = 10.55YY34 pKa = 9.67FTEE37 pKa = 4.48LDD39 pKa = 3.53NIITHH44 pKa = 6.82KK45 pKa = 10.75LLSFDD50 pKa = 4.03FEE52 pKa = 4.81YY53 pKa = 11.05NIYY56 pKa = 10.4GKK58 pKa = 10.24YY59 pKa = 9.94VEE61 pKa = 5.71DD62 pKa = 5.27DD63 pKa = 3.18IKK65 pKa = 11.29VYY67 pKa = 10.19HH68 pKa = 6.61ANAWSKK74 pKa = 10.67FIGSHH79 pKa = 6.38CPPNHH84 pKa = 6.46HH85 pKa = 6.47KK86 pKa = 10.87VSLQHH91 pKa = 6.72HH92 pKa = 6.93IFSEE96 pKa = 5.1LYY98 pKa = 9.46VCIHH102 pKa = 6.12VPTVLKK108 pKa = 10.71NPHH111 pKa = 5.74SVYY114 pKa = 10.58KK115 pKa = 10.48INKK118 pKa = 8.28NYY120 pKa = 10.89NEE122 pKa = 4.21VLVSCYY128 pKa = 10.58LPGGDD133 pKa = 3.72EE134 pKa = 4.17FAIEE138 pKa = 4.66TIRR141 pKa = 11.84VDD143 pKa = 4.98IPDD146 pKa = 3.82LDD148 pKa = 3.49KK149 pKa = 11.24FRR151 pKa = 11.84IIEE154 pKa = 4.1FNGKK158 pKa = 8.97NFLTSQYY165 pKa = 9.85CISDD169 pKa = 3.81RR170 pKa = 11.84YY171 pKa = 11.11GLTSDD176 pKa = 3.66HH177 pKa = 7.15KK178 pKa = 11.12EE179 pKa = 4.0VLQVNYY185 pKa = 9.4TVTDD189 pKa = 3.53NTISFQFPKK198 pKa = 10.76GEE200 pKa = 4.11CRR202 pKa = 11.84KK203 pKa = 10.27DD204 pKa = 3.33SQLIWDD210 pKa = 4.78IDD212 pKa = 4.02SLHH215 pKa = 6.88PGYY218 pKa = 10.69YY219 pKa = 10.07LPFVINITASTNMHH233 pKa = 6.35KK234 pKa = 10.6CLTYY238 pKa = 10.7YY239 pKa = 7.3YY240 pKa = 9.67TPYY243 pKa = 9.9DD244 pKa = 3.73TEE246 pKa = 5.51LDD248 pKa = 3.88TNVTVLPLTPTEE260 pKa = 3.98HH261 pKa = 7.07LIWAVDD267 pKa = 3.47VSPIIVDD274 pKa = 3.3ARR276 pKa = 11.84NLTRR280 pKa = 11.84VWSSINYY287 pKa = 7.77YY288 pKa = 9.61PPPSDD293 pKa = 3.28TVIHH297 pKa = 6.57IGSAITNSPFNFISSAILAILRR319 pKa = 11.84PIIDD323 pKa = 3.85ILLDD327 pKa = 3.35SLIYY331 pKa = 10.22ILSTLMEE338 pKa = 4.3IFEE341 pKa = 4.33SSEE344 pKa = 3.31FLEE347 pKa = 4.23IVNRR351 pKa = 11.84LFDD354 pKa = 4.45FVYY357 pKa = 11.08LLIKK361 pKa = 10.68NIFEE365 pKa = 4.91FIVKK369 pKa = 9.87VIYY372 pKa = 9.6PRR374 pKa = 11.84LLDD377 pKa = 3.44IVLSFSTKK385 pKa = 9.94CKK387 pKa = 9.69FLFVVFIITYY397 pKa = 9.22IKK399 pKa = 9.0TGKK402 pKa = 10.27FFVTCIITFFVNMCMRR418 pKa = 11.84EE419 pKa = 3.96RR420 pKa = 11.84EE421 pKa = 4.23VV422 pKa = 3.15

Molecular weight: 49.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W5VNC4|W5VNC4_9VIRU Uncharacterized protein OS=Wallerfield virus OX=1457165 PE=4 SV=1
MM1 pKa = 7.68ALQRR5 pKa = 11.84TGTVVKK11 pKa = 9.67PRR13 pKa = 11.84AVAARR18 pKa = 11.84TVSAPRR24 pKa = 11.84TATVKK29 pKa = 10.92AKK31 pKa = 9.02LTRR34 pKa = 11.84LRR36 pKa = 11.84EE37 pKa = 4.08SANLDD42 pKa = 3.2FSKK45 pKa = 10.9FFDD48 pKa = 3.85YY49 pKa = 9.26ATNILTDD56 pKa = 3.34TTFMVFFVLSAYY68 pKa = 10.86LCFNYY73 pKa = 7.54TTKK76 pKa = 9.84GTSSMLVAFVDD87 pKa = 3.55NFIKK91 pKa = 10.6HH92 pKa = 6.03FSSFSDD98 pKa = 3.49SKK100 pKa = 11.32CSILGMLLVGIPFAPAILTVNPKK123 pKa = 9.97NRR125 pKa = 11.84FGAILCTVLYY135 pKa = 10.36YY136 pKa = 10.57VFIPEE141 pKa = 4.11RR142 pKa = 11.84TVYY145 pKa = 10.33EE146 pKa = 4.24YY147 pKa = 10.79LVHH150 pKa = 6.61GALVYY155 pKa = 10.76LILRR159 pKa = 11.84TSNKK163 pKa = 7.4QFKK166 pKa = 10.45LIGVGLLFLSYY177 pKa = 11.09VMQFAIPLPSGDD189 pKa = 4.03GYY191 pKa = 11.14SCNVTKK197 pKa = 10.88SEE199 pKa = 4.0

Molecular weight: 22.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2883

199

2262

961.0

110.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.787 ± 0.636

2.567 ± 0.143

6.035 ± 0.597

5.168 ± 0.639

5.793 ± 0.871

2.983 ± 0.339

3.087 ± 0.336

8.221 ± 0.878

6.07 ± 0.348

8.845 ± 0.672

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.16

5.862 ± 0.41

4.266 ± 0.071

2.393 ± 0.505

4.405 ± 0.361

7.666 ± 0.097

6.382 ± 0.568

7.215 ± 0.434

0.451 ± 0.149

5.619 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski