Erwinia phage vB_EamM_Bosolaphorus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Agricanvirus; unclassified Agricanvirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 320 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5BHK1|A0A2H5BHK1_9CAUD Putative membrane protein OS=Erwinia phage vB_EamM_Bosolaphorus OX=2060126 GN=BOSOLAPHORUS_18 PE=4 SV=1
MM1 pKa = 7.88PANQPIHH8 pKa = 6.9GEE10 pKa = 3.64GDD12 pKa = 3.87YY13 pKa = 11.37VVDD16 pKa = 3.86VALSSAEE23 pKa = 3.95LVYY26 pKa = 11.18GLINHH31 pKa = 7.29DD32 pKa = 4.08NNTDD36 pKa = 3.64YY37 pKa = 11.49TSLTLRR43 pKa = 11.84LGTPTVNTNAQHH55 pKa = 6.82DD56 pKa = 4.1RR57 pKa = 11.84NTRR60 pKa = 11.84IFAQTRR66 pKa = 11.84PGAPKK71 pKa = 9.98QGSTYY76 pKa = 10.23FYY78 pKa = 10.5YY79 pKa = 10.82DD80 pKa = 4.35RR81 pKa = 11.84ISLAYY86 pKa = 9.97YY87 pKa = 8.48LQNYY91 pKa = 7.84PDD93 pKa = 4.73LEE95 pKa = 4.14VDD97 pKa = 3.19ITDD100 pKa = 5.12DD101 pKa = 3.61MLTMRR106 pKa = 11.84DD107 pKa = 4.52LIPWMNQTFGLGLVAEE123 pKa = 4.99DD124 pKa = 4.06VRR126 pKa = 11.84ADD128 pKa = 4.73LIDD131 pKa = 3.63RR132 pKa = 11.84TPNAEE137 pKa = 3.64NRR139 pKa = 11.84FAMSAASMAWQDD151 pKa = 4.61SIVLKK156 pKa = 10.8INIPPVGIATLTGDD170 pKa = 3.88SVFDD174 pKa = 4.67AIEE177 pKa = 4.16VVSEE181 pKa = 3.94HH182 pKa = 6.55PRR184 pKa = 11.84TYY186 pKa = 10.41GQLYY190 pKa = 9.5GYY192 pKa = 10.23QVDD195 pKa = 4.09TSGMAALLTGLPIGKK210 pKa = 9.67LSNLNGEE217 pKa = 4.78NLTQALSTATSDD229 pKa = 3.16YY230 pKa = 10.04WAFDD234 pKa = 3.15ATPVVRR240 pKa = 11.84NLYY243 pKa = 9.73GATVRR248 pKa = 11.84FNGRR252 pKa = 11.84STDD255 pKa = 3.51QAVYY259 pKa = 7.86PTKK262 pKa = 10.12PDD264 pKa = 3.15NAYY267 pKa = 9.39MVIVDD272 pKa = 3.85IVEE275 pKa = 4.3SEE277 pKa = 4.14CLEE280 pKa = 4.13FEE282 pKa = 4.66GPLVLYY288 pKa = 7.29YY289 pKa = 10.48TRR291 pKa = 5.02

Molecular weight: 32.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5BHV2|A0A2H5BHV2_9CAUD Dihydrofolate reductase OS=Erwinia phage vB_EamM_Bosolaphorus OX=2060126 GN=BOSOLAPHORUS_123 PE=4 SV=1
MM1 pKa = 7.94RR2 pKa = 11.84IDD4 pKa = 3.78TLSCYY9 pKa = 10.12DD10 pKa = 3.35GRR12 pKa = 11.84IPRR15 pKa = 11.84PALGVRR21 pKa = 11.84SMSIAVNRR29 pKa = 11.84HH30 pKa = 3.67HH31 pKa = 7.7AKK33 pKa = 10.14RR34 pKa = 11.84LKK36 pKa = 10.49AKK38 pKa = 10.08RR39 pKa = 11.84KK40 pKa = 9.51HH41 pKa = 4.77YY42 pKa = 9.96WNVGGKK48 pKa = 8.88RR49 pKa = 11.84QNNKK53 pKa = 9.13AVGIIFHH60 pKa = 6.24TPCKK64 pKa = 9.99CSCTMCGNQRR74 pKa = 11.84KK75 pKa = 8.25HH76 pKa = 5.28WGRR79 pKa = 11.84PIQEE83 pKa = 4.08VKK85 pKa = 9.84QAARR89 pKa = 11.84FSDD92 pKa = 3.79TVTDD96 pKa = 3.51II97 pKa = 4.19

Molecular weight: 11.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

320

0

320

84490

41

2234

264.0

29.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.202 ± 0.167

0.882 ± 0.046

6.24 ± 0.152

5.751 ± 0.134

3.586 ± 0.066

6.806 ± 0.305

2.159 ± 0.085

5.912 ± 0.122

5.292 ± 0.115

8.873 ± 0.126

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.979 ± 0.086

5.119 ± 0.093

4.306 ± 0.092

4.077 ± 0.125

4.999 ± 0.117

5.927 ± 0.119

6.569 ± 0.126

7.226 ± 0.124

1.207 ± 0.046

3.888 ± 0.099
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski