Carnation cryptic virus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; unclassified Partitiviridae

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6C2H2|A0A1W6C2H2_9VIRU Putative RNA-dependent RNA polymerase OS=Carnation cryptic virus 3 OX=1980625 PE=4 SV=1
MM1 pKa = 7.82AEE3 pKa = 3.88NDD5 pKa = 3.5IPAEE9 pKa = 4.6GIPARR14 pKa = 11.84PPPAANPFTGLNRR27 pKa = 11.84DD28 pKa = 3.37EE29 pKa = 5.07LDD31 pKa = 4.32RR32 pKa = 11.84IVEE35 pKa = 4.07QPEE38 pKa = 3.64EE39 pKa = 4.22DD40 pKa = 3.56NQIGLFRR47 pKa = 11.84DD48 pKa = 3.31KK49 pKa = 10.79ARR51 pKa = 11.84SYY53 pKa = 10.86RR54 pKa = 11.84RR55 pKa = 11.84YY56 pKa = 9.72VDD58 pKa = 4.49LRR60 pKa = 11.84TSPLSTRR67 pKa = 11.84LINLYY72 pKa = 10.92SNLFTTHH79 pKa = 5.21WNVFKK84 pKa = 9.99HH85 pKa = 5.02TMSGEE90 pKa = 4.04APAGFASKK98 pKa = 10.55AIYY101 pKa = 9.87LATIYY106 pKa = 10.57VSAWIWDD113 pKa = 3.42LHH115 pKa = 6.48VSIRR119 pKa = 11.84EE120 pKa = 3.98SVRR123 pKa = 11.84RR124 pKa = 11.84ISGTAFNQLFQQDD137 pKa = 4.39AYY139 pKa = 10.24HH140 pKa = 6.09QADD143 pKa = 4.12RR144 pKa = 11.84YY145 pKa = 11.32DD146 pKa = 3.88PFLQHH151 pKa = 7.02LNTIIRR157 pKa = 11.84PTPIHH162 pKa = 6.03QALEE166 pKa = 4.01DD167 pKa = 3.53TLYY170 pKa = 10.68IPVVSRR176 pKa = 11.84NIDD179 pKa = 3.04WANANNNVFGLPSHH193 pKa = 7.02AYY195 pKa = 10.05DD196 pKa = 5.21DD197 pKa = 5.09NILNAILDD205 pKa = 3.88VMDD208 pKa = 4.78DD209 pKa = 5.13RR210 pKa = 11.84RR211 pKa = 11.84NQWSTIPISTNVLGRR226 pKa = 11.84PGWLLDD232 pKa = 3.48YY233 pKa = 10.96RR234 pKa = 11.84LGQAYY239 pKa = 9.88AWFPMEE245 pKa = 4.76ANYY248 pKa = 10.89SQVDD252 pKa = 4.87LIAPHH257 pKa = 7.05ILGTPCTPRR266 pKa = 11.84LGPRR270 pKa = 11.84DD271 pKa = 3.57KK272 pKa = 11.36DD273 pKa = 3.01DD274 pKa = 3.47WQVFRR279 pKa = 11.84YY280 pKa = 9.17NAAPPPGVRR289 pKa = 11.84LNPHH293 pKa = 6.01DD294 pKa = 3.91FRR296 pKa = 11.84RR297 pKa = 11.84ATEE300 pKa = 3.75RR301 pKa = 11.84SFYY304 pKa = 11.11GSAEE308 pKa = 3.79YY309 pKa = 8.48RR310 pKa = 11.84TIEE313 pKa = 4.19HH314 pKa = 6.59AVHH317 pKa = 6.03VFDD320 pKa = 4.28WQATIPPPDD329 pKa = 3.88AATSAASQPGKK340 pKa = 10.24RR341 pKa = 11.84KK342 pKa = 10.05QGDD345 pKa = 3.58SSTSAMALMEE355 pKa = 4.22TDD357 pKa = 4.2KK358 pKa = 11.31DD359 pKa = 3.81PPQPFVPPPPVDD371 pKa = 3.73LNRR374 pKa = 11.84YY375 pKa = 8.91RR376 pKa = 11.84LIDD379 pKa = 3.37WCYY382 pKa = 8.17HH383 pKa = 4.69ARR385 pKa = 11.84VIDD388 pKa = 4.71GEE390 pKa = 4.17DD391 pKa = 3.08QQTRR395 pKa = 11.84NRR397 pKa = 11.84ALRR400 pKa = 11.84NFIFQAGHH408 pKa = 5.57QPTT411 pKa = 3.65

Molecular weight: 46.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6C2H2|A0A1W6C2H2_9VIRU Putative RNA-dependent RNA polymerase OS=Carnation cryptic virus 3 OX=1980625 PE=4 SV=1
MM1 pKa = 7.42HH2 pKa = 7.65NINNYY7 pKa = 9.3EE8 pKa = 3.86FTDD11 pKa = 3.85FTEE14 pKa = 4.54EE15 pKa = 3.98VEE17 pKa = 4.44EE18 pKa = 4.19TGRR21 pKa = 11.84THH23 pKa = 5.53HH24 pKa = 6.07HH25 pKa = 4.55VVRR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 3.98PEE32 pKa = 3.6VTYY35 pKa = 10.65EE36 pKa = 4.8DD37 pKa = 4.3RR38 pKa = 11.84FASQVLRR45 pKa = 11.84HH46 pKa = 6.47KK47 pKa = 11.28YY48 pKa = 8.74PVLYY52 pKa = 10.61EE53 pKa = 4.13NIISGWSRR61 pKa = 11.84SYY63 pKa = 11.44YY64 pKa = 10.63SGAEE68 pKa = 3.84HH69 pKa = 6.65MKK71 pKa = 10.69SIMQYY76 pKa = 8.11ATPNIKK82 pKa = 10.32LEE84 pKa = 4.04RR85 pKa = 11.84LHH87 pKa = 7.64DD88 pKa = 4.21GVYY91 pKa = 10.43HH92 pKa = 6.61KK93 pKa = 10.89AIANVRR99 pKa = 11.84EE100 pKa = 4.36SLRR103 pKa = 11.84SLPTVRR109 pKa = 11.84AFNVLNEE116 pKa = 4.13LDD118 pKa = 4.06LVPFEE123 pKa = 4.8SSSSAGYY130 pKa = 10.52DD131 pKa = 3.31YY132 pKa = 11.35TGVKK136 pKa = 10.37GPMYY140 pKa = 10.64GEE142 pKa = 3.72NHH144 pKa = 5.37EE145 pKa = 4.21RR146 pKa = 11.84AIRR149 pKa = 11.84RR150 pKa = 11.84AKK152 pKa = 8.59ATLWSAIALDD162 pKa = 4.25GEE164 pKa = 5.11GIDD167 pKa = 3.73HH168 pKa = 7.61VIRR171 pKa = 11.84TYY173 pKa = 11.14VPDD176 pKa = 3.19VGYY179 pKa = 8.69TRR181 pKa = 11.84TQLTDD186 pKa = 3.16LRR188 pKa = 11.84EE189 pKa = 4.0KK190 pKa = 10.37MKK192 pKa = 10.7VRR194 pKa = 11.84GVWGRR199 pKa = 11.84AFHH202 pKa = 6.64YY203 pKa = 10.18ILLEE207 pKa = 4.14GVVAAPLLEE216 pKa = 4.66AFSSADD222 pKa = 3.39TFFHH226 pKa = 6.59IGKK229 pKa = 9.29DD230 pKa = 3.42PTVSVPYY237 pKa = 10.21LLSTIKK243 pKa = 9.62STAEE247 pKa = 3.72WITAIDD253 pKa = 3.74WQSFDD258 pKa = 3.23ATVSRR263 pKa = 11.84FEE265 pKa = 4.11INAAFDD271 pKa = 4.0IIEE274 pKa = 5.38DD275 pKa = 3.41IVSFPNFEE283 pKa = 3.96TEE285 pKa = 3.82QAFEE289 pKa = 3.95ISRR292 pKa = 11.84QLFIHH297 pKa = 6.57KK298 pKa = 9.19MLAAPDD304 pKa = 3.77GKK306 pKa = 10.41IYY308 pKa = 9.8WIHH311 pKa = 6.86KK312 pKa = 7.48GIPSGSYY319 pKa = 7.41FTSTIGSLVNRR330 pKa = 11.84LRR332 pKa = 11.84IEE334 pKa = 4.12YY335 pKa = 8.48MWILKK340 pKa = 9.84FDD342 pKa = 4.63RR343 pKa = 11.84GPKK346 pKa = 8.95ICYY349 pKa = 7.43TQGDD353 pKa = 4.13DD354 pKa = 5.01SLIGDD359 pKa = 4.94DD360 pKa = 4.6EE361 pKa = 5.16LFSPMDD367 pKa = 3.19MTFLVKK373 pKa = 10.17PYY375 pKa = 9.83NWLINVTKK383 pKa = 10.85SMASKK388 pKa = 10.21IPEE391 pKa = 3.85AVTFLGRR398 pKa = 11.84TSIGGLNQRR407 pKa = 11.84DD408 pKa = 4.02LKK410 pKa = 10.89RR411 pKa = 11.84CLRR414 pKa = 11.84LLILPEE420 pKa = 4.1YY421 pKa = 9.34PVTSGDD427 pKa = 2.93ISAYY431 pKa = 8.23RR432 pKa = 11.84AKK434 pKa = 10.66AIWYY438 pKa = 8.85DD439 pKa = 3.33SGLTSEE445 pKa = 4.63VLGEE449 pKa = 3.67IARR452 pKa = 11.84ALRR455 pKa = 11.84RR456 pKa = 11.84KK457 pKa = 10.02YY458 pKa = 10.86GLAEE462 pKa = 3.81KK463 pKa = 10.69SKK465 pKa = 9.88VPKK468 pKa = 10.6YY469 pKa = 10.21LIPWKK474 pKa = 10.7GG475 pKa = 2.94

Molecular weight: 54.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

886

411

475

443.0

50.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.126 ± 0.919

0.451 ± 0.024

6.433 ± 0.912

5.418 ± 1.192

4.289 ± 0.103

5.418 ± 0.7

3.047 ± 0.078

7.111 ± 0.529

3.612 ± 1.287

7.901 ± 0.405

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.806 ± 0.233

4.402 ± 0.969

6.546 ± 1.656

3.16 ± 1.15

7.336 ± 0.631

6.546 ± 0.805

5.869 ± 0.184

5.53 ± 0.612

2.257 ± 0.118

4.74 ± 0.407
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski